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The map label for this gene is araF [H]

Identifier: 13475901

GI number: 13475901

Start: 5845524

End: 5846507

Strand: Reverse

Name: araF [H]

Synonym: mll7082

Alternate gene names: 13475901

Gene position: 5846507-5845524 (Counterclockwise)

Preceding gene: 13475902

Following gene: 13475900

Centisome position: 83.09

GC content: 61.08

Gene sequence:

>984_bases
ATGAAAACGACGACAAAGGCTCTGGCAACAGTGGCGATATTGGCCGCCGGACTGATGGCTGGCGCGGCAAGCGCCGACGA
CATCAAGATCGGCTACATCAACAAGATGGGCGAGCATCCATGGTTCGTCGCCGAAGTCGGCGGCGCCAAGAAAGCGGCCG
AGGCGGCCGGCGCCACCTTCGTGTCGCAAGACGTCCAGTTCGACTCCAATCTGACGATCACCACCTTCGACACGATGGTC
GGTGACGGCGTCAAGGGCATCGCAATCGTCGTTCCCGACAAGGCGCTGGGACCGGTCATCGCCGAGAAGGCCAAGGCGGC
GAAAATCCCCCTGGTGGCGGTCGATGACGATATCTACTACCAGGACGGCACCCAGGTTCCCTATGTCGGCATGAATGCCT
ATAATATCGGTGCGCGGGTGGGCGAAGAACTGGCCAAGCTCTACAAGGCCGATGGCTGGGCCGGCAAGGAGGTGCGTATC
GCCTCGATCGAGGACCGCAAGGCCGACACCTGCATGCAGCGCAACAAGGGTGCCGAGGAAGCGTTCCTGAAGGCAGTGCC
CGATTTCAAGCCGGCAAACATCCTTCGCGTCCCCTATGACAACACGATGGTCAACGCCATCGACGTCATGACCACCACAT
TGACGGCCAATCCCGGCGTCACCAACTGGATCTTCTATTCCTGCAACGATGACGGCGTGTTGGGTGCGGCGCGCGCGCTG
GAAAACGCCGGCTATGCCGCGGACCAGGGCATGGGCATCGGCATTGACGGCAGCCGCGCTTGCGAGGCCTTCGGATCAGG
AAAGCCCTCGGCATTCCGTGGTACGATGTGGCTCAATTCGGCAAACCATGGCGCGCTGGCGGTGAAGATCCTGCTGGCCT
CGATCAAGGACGGAACGAAGCTGCCCGTTCAGTCCTTCACGGACCCGGAATACATCACCGCCGGCAATTTTGCATCGGAC
TACAAGGCCAAGCTCTGCCACTGA

Upstream 100 bases:

>100_bases
GCTTCGGACGAAGCGCCGTTCATCAATGCCGAGACGATCACCATCGATGGTGGACGCTCGGTTCTCTACCATGACTGACA
ACCAAGCAGGGAGGATCCAC

Downstream 100 bases:

>100_bases
GCCGGACCTGCGTCATGCGCCTCCAGGGCGCATGACGCTCTTCAACTGCTCGGATCGATCCTTGGGAGTGAGCTTTGACG
CAATCGGACTTCTTCTCGCT

Product: L-arabinose-binding protein

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKTTTKALATVAILAAGLMAGAASADDIKIGYINKMGEHPWFVAEVGGAKKAAEAAGATFVSQDVQFDSNLTITTFDTMV
GDGVKGIAIVVPDKALGPVIAEKAKAAKIPLVAVDDDIYYQDGTQVPYVGMNAYNIGARVGEELAKLYKADGWAGKEVRI
ASIEDRKADTCMQRNKGAEEAFLKAVPDFKPANILRVPYDNTMVNAIDVMTTTLTANPGVTNWIFYSCNDDGVLGAARAL
ENAGYAADQGMGIGIDGSRACEAFGSGKPSAFRGTMWLNSANHGALAVKILLASIKDGTKLPVQSFTDPEYITAGNFASD
YKAKLCH

Sequences:

>Translated_327_residues
MKTTTKALATVAILAAGLMAGAASADDIKIGYINKMGEHPWFVAEVGGAKKAAEAAGATFVSQDVQFDSNLTITTFDTMV
GDGVKGIAIVVPDKALGPVIAEKAKAAKIPLVAVDDDIYYQDGTQVPYVGMNAYNIGARVGEELAKLYKADGWAGKEVRI
ASIEDRKADTCMQRNKGAEEAFLKAVPDFKPANILRVPYDNTMVNAIDVMTTTLTANPGVTNWIFYSCNDDGVLGAARAL
ENAGYAADQGMGIGIDGSRACEAFGSGKPSAFRGTMWLNSANHGALAVKILLASIKDGTKLPVQSFTDPEYITAGNFASD
YKAKLCH
>Mature_327_residues
MKTTTKALATVAILAAGLMAGAASADDIKIGYINKMGEHPWFVAEVGGAKKAAEAAGATFVSQDVQFDSNLTITTFDTMV
GDGVKGIAIVVPDKALGPVIAEKAKAAKIPLVAVDDDIYYQDGTQVPYVGMNAYNIGARVGEELAKLYKADGWAGKEVRI
ASIEDRKADTCMQRNKGAEEAFLKAVPDFKPANILRVPYDNTMVNAIDVMTTTLTANPGVTNWIFYSCNDDGVLGAARAL
ENAGYAADQGMGIGIDGSRACEAFGSGKPSAFRGTMWLNSANHGALAVKILLASIKDGTKLPVQSFTDPEYITAGNFASD
YKAKLCH

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=329, Percent_Identity=32.5227963525836, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34449; Mature: 34449

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTTKALATVAILAAGLMAGAASADDIKIGYINKMGEHPWFVAEVGGAKKAAEAAGATF
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHCCEE
VSQDVQFDSNLTITTFDTMVGDGVKGIAIVVPDKALGPVIAEKAKAAKIPLVAVDDDIYY
EECCCEECCCEEEEEEHHHHCCCCCEEEEEECCHHHCHHHHHHHHHHCCCEEEECCCEEE
QDGTQVPYVGMNAYNIGARVGEELAKLYKADGWAGKEVRIASIEDRKADTCMQRNKGAEE
CCCCCCCEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCHHH
AFLKAVPDFKPANILRVPYDNTMVNAIDVMTTTLTANPGVTNWIFYSCNDDGVLGAARAL
HHHHHCCCCCCCCEEECCCCCCHHHHEEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHH
ENAGYAADQGMGIGIDGSRACEAFGSGKPSAFRGTMWLNSANHGALAVKILLASIKDGTK
HHCCCCCCCCCEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCHHEEEEHHHHHCCCCC
LPVQSFTDPEYITAGNFASDYKAKLCH
CCCCCCCCCCEEECCCCCCCCHHHCCC
>Mature Secondary Structure
MKTTTKALATVAILAAGLMAGAASADDIKIGYINKMGEHPWFVAEVGGAKKAAEAAGATF
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHCCEE
VSQDVQFDSNLTITTFDTMVGDGVKGIAIVVPDKALGPVIAEKAKAAKIPLVAVDDDIYY
EECCCEECCCEEEEEEHHHHCCCCCEEEEEECCHHHCHHHHHHHHHHCCCEEEECCCEEE
QDGTQVPYVGMNAYNIGARVGEELAKLYKADGWAGKEVRIASIEDRKADTCMQRNKGAEE
CCCCCCCEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCHHH
AFLKAVPDFKPANILRVPYDNTMVNAIDVMTTTLTANPGVTNWIFYSCNDDGVLGAARAL
HHHHHCCCCCCCCEEECCCCCCHHHHEEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHH
ENAGYAADQGMGIGIDGSRACEAFGSGKPSAFRGTMWLNSANHGALAVKILLASIKDGTK
HHCCCCCCCCCEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCHHEEEEHHHHHCCCCC
LPVQSFTDPEYITAGNFASDYKAKLCH
CCCCCCCCCCEEECCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]