Click here to switch to the map view.

The map label for this gene is potD-A [H]

Identifier: 13475793

GI number: 13475793

Start: 5723382

End: 5724464

Strand: Direct

Name: potD-A [H]

Synonym: mlr6964

Alternate gene names: 13475793

Gene position: 5723382-5724464 (Clockwise)

Preceding gene: 13475792

Following gene: 13475794

Centisome position: 81.34

GC content: 53.37

Gene sequence:

>1083_bases
GTGCTGAAGCGTTGGAACACCGGACTATCGAGCCAGAAACCAAGCGAGAGCCAAATGAAACTCACCGCAACGTCCGCCGC
GTTAATTCTGGGCCTGAGCACCACCGTCGCCCTGGCTGAAGGCAATCTCAACATCTACAATTGGGGCGAGTACACCAGCC
CCGAATTGATCGACAAATTCTCAAAGACCTACAACATCCATGTGACGCAGACGGATTTCGATTCCAACGACACCGCACTT
GCCAAGGTGCGTCAGGGTTCCTCCGGGTTCGACGTCGTGGTTCCCTCGCAGAGCTTCATCCCCACCTATATTCAAGAAGG
GCTGCTTGCCGAGACAAATCCGGGGCAGATGGAAAATGCCAAGAACCTCGAAGAGAGGTGGCGGAATCCCGCTTTCGACC
CAGGACGAAAATATTCCGTGCCGTGGCTTTGGTATACCAGCGGCGTAAGCGTCAATACGTCCGTCTTCAAAGGCGACATA
AATACCTGGAAGGTGATTCTGGATCCGCCGGCTGAATTGAAGGGAAAGATCAATATCGTTCCCGAGATGAACGATATCAT
GTTCGCCGCGATCAAGTTCGAAGGCGGAACATGGTGCACAAGCGATAAGGCCTTGCTCACCAAGGTCCGCGACCGTCTCC
TGGAGGCCAAGAAGAGCTGGCTGTCGATCGACTATTCCGGAACACTGAAAATGGCCTCCGGAGATGTTTCGGCTTCGCTT
GACTGGAGTGGTTCCGCGTTGAAGCGCCGTACTCAAAATCACAGCATCGCCTATGGCCTTCCCAAAGAGGGCTTCACCTA
CGGCTCTGACAATGTGGTGGTTCTGAAGGATGCTCCAAATCTGGAGAACGCCAAGCTTTTTCAAAACTTCATTATGGCGC
CCGAAAACGCGGCATTGAATTCGACCTTTGCCAAATATGGCGCGGCTATCATCGGTGCTGAAAAATACTACTCGGACGAT
ATGAAGGGTGCGCCGGAGCTGACCATTCCCGACGATATGAAATCAAAGGGTGAGCTTCTGACGCTTTGCGACCCGAAGAT
CACGCAGCTCTACAGCCGTATCTGGCAGGACGTCCAGAAGTAG

Upstream 100 bases:

>100_bases
CCGCCTGCCTTCCTTTTAGGCAGGGCAGATGGCTAGACGACAACCAACCAGCCTGAACATTTTTGAAGCCGGGCCTGAAG
CTCCATCGGATGCCCTTCAG

Downstream 100 bases:

>100_bases
CTGCTTCTATCGGTCCGCTGGCGATGTTTGCGCTGCATTCGACGACAGCGTTTCCTATCGGCGGCATAACTCCTGAGTGA
TTCATTTTGGGGACACTATG

Product: ABC transporter, binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MLKRWNTGLSSQKPSESQMKLTATSAALILGLSTTVALAEGNLNIYNWGEYTSPELIDKFSKTYNIHVTQTDFDSNDTAL
AKVRQGSSGFDVVVPSQSFIPTYIQEGLLAETNPGQMENAKNLEERWRNPAFDPGRKYSVPWLWYTSGVSVNTSVFKGDI
NTWKVILDPPAELKGKINIVPEMNDIMFAAIKFEGGTWCTSDKALLTKVRDRLLEAKKSWLSIDYSGTLKMASGDVSASL
DWSGSALKRRTQNHSIAYGLPKEGFTYGSDNVVVLKDAPNLENAKLFQNFIMAPENAALNSTFAKYGAAIIGAEKYYSDD
MKGAPELTIPDDMKSKGELLTLCDPKITQLYSRIWQDVQK

Sequences:

>Translated_360_residues
MLKRWNTGLSSQKPSESQMKLTATSAALILGLSTTVALAEGNLNIYNWGEYTSPELIDKFSKTYNIHVTQTDFDSNDTAL
AKVRQGSSGFDVVVPSQSFIPTYIQEGLLAETNPGQMENAKNLEERWRNPAFDPGRKYSVPWLWYTSGVSVNTSVFKGDI
NTWKVILDPPAELKGKINIVPEMNDIMFAAIKFEGGTWCTSDKALLTKVRDRLLEAKKSWLSIDYSGTLKMASGDVSASL
DWSGSALKRRTQNHSIAYGLPKEGFTYGSDNVVVLKDAPNLENAKLFQNFIMAPENAALNSTFAKYGAAIIGAEKYYSDD
MKGAPELTIPDDMKSKGELLTLCDPKITQLYSRIWQDVQK
>Mature_360_residues
MLKRWNTGLSSQKPSESQMKLTATSAALILGLSTTVALAEGNLNIYNWGEYTSPELIDKFSKTYNIHVTQTDFDSNDTAL
AKVRQGSSGFDVVVPSQSFIPTYIQEGLLAETNPGQMENAKNLEERWRNPAFDPGRKYSVPWLWYTSGVSVNTSVFKGDI
NTWKVILDPPAELKGKINIVPEMNDIMFAAIKFEGGTWCTSDKALLTKVRDRLLEAKKSWLSIDYSGTLKMASGDVSASL
DWSGSALKRRTQNHSIAYGLPKEGFTYGSDNVVVLKDAPNLENAKLFQNFIMAPENAALNSTFAKYGAAIIGAEKYYSDD
MKGAPELTIPDDMKSKGELLTLCDPKITQLYSRIWQDVQK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=365, Percent_Identity=27.6712328767123, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787367, Length=294, Percent_Identity=26.8707482993197, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39918; Mature: 39918

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRWNTGLSSQKPSESQMKLTATSAALILGLSTTVALAEGNLNIYNWGEYTSPELIDKF
CCCCCCCCCCCCCCCCCCEEEEEHHEEEEEEECEEEEEECCCEEEEECCCCCCHHHHHHH
SKTYNIHVTQTDFDSNDTALAKVRQGSSGFDVVVPSQSFIPTYIQEGLLAETNPGQMENA
HCEEEEEEEECCCCCCCCEEHHHHCCCCCCEEEECCCCCCHHHHHCCCEECCCCCCCCCH
KNLEERWRNPAFDPGRKYSVPWLWYTSGVSVNTSVFKGDINTWKVILDPPAELKGKINIV
HHHHHHHCCCCCCCCCCCCCCEEEEECCCEEECCEEECCCCEEEEEECCCHHCCCEEEEE
PEMNDIMFAAIKFEGGTWCTSDKALLTKVRDRLLEAKKSWLSIDYSGTLKMASGDVSASL
ECCCCEEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEE
DWSGSALKRRTQNHSIAYGLPKEGFTYGSDNVVVLKDAPNLENAKLFQNFIMAPENAALN
CCCCHHHHHHHCCCCEEECCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHH
STFAKYGAAIIGAEKYYSDDMKGAPELTIPDDMKSKGELLTLCDPKITQLYSRIWQDVQK
HHHHHHCCEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKRWNTGLSSQKPSESQMKLTATSAALILGLSTTVALAEGNLNIYNWGEYTSPELIDKF
CCCCCCCCCCCCCCCCCCEEEEEHHEEEEEEECEEEEEECCCEEEEECCCCCCHHHHHHH
SKTYNIHVTQTDFDSNDTALAKVRQGSSGFDVVVPSQSFIPTYIQEGLLAETNPGQMENA
HCEEEEEEEECCCCCCCCEEHHHHCCCCCCEEEECCCCCCHHHHHCCCEECCCCCCCCCH
KNLEERWRNPAFDPGRKYSVPWLWYTSGVSVNTSVFKGDINTWKVILDPPAELKGKINIV
HHHHHHHCCCCCCCCCCCCCCEEEEECCCEEECCEEECCCCEEEEEECCCHHCCCEEEEE
PEMNDIMFAAIKFEGGTWCTSDKALLTKVRDRLLEAKKSWLSIDYSGTLKMASGDVSASL
ECCCCEEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEE
DWSGSALKRRTQNHSIAYGLPKEGFTYGSDNVVVLKDAPNLENAKLFQNFIMAPENAALN
CCCCHHHHHHHCCCCEEECCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHH
STFAKYGAAIIGAEKYYSDDMKGAPELTIPDDMKSKGELLTLCDPKITQLYSRIWQDVQK
HHHHHHCCEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]