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The map label for this gene is intZ [H]

Identifier: 13475785

GI number: 13475785

Start: 5714223

End: 5715485

Strand: Direct

Name: intZ [H]

Synonym: mlr6952

Alternate gene names: 13475785

Gene position: 5714223-5715485 (Clockwise)

Preceding gene: 13475784

Following gene: 13475788

Centisome position: 81.21

GC content: 58.83

Gene sequence:

>1263_bases
ATGGGTATGTTGACGGACGTCGCCCTTAAGGCACTGAAACCGAAAGAAAAAATCTACAAGATCGCTGACCGTGACGGCAT
ATACGTTCGCGTTATGCCCAGTGGCGCGATCTCGTTTCGGCTGGATTACAGGCTCAATGGACGCCGTGAGACGATCTATC
TAGGCAGATATGGCAGAGACGGGATCTCGCTCGCTTTGGCCCGAGAGAAGTGCCTCGACGCGAAGCGCGCCATTGGCGAG
GGGCGATCGCCGGCGATCGAGAAGCAGCGTGACAAGCGTCGCCTGAAAGAGGCTAAGAGTTTTGGCGAGTTCGGGGAGAA
GTGGCTCACCGCGGCGCCGATGGCCGACAGCACGCGCGCCATGCGGCGCGCCATCTTCGAGCGGGAGCTTCTTCCGGTCT
GGCGCAATCGCCTGCTGACGGAGATTACGCCGGATGACCTGCGGACCCATTGCGGCGCTATTGTTGAGCGTGGCGCTCCG
GCTACCGCCATCCACGTTCGAGACATCCTGAAGCAAATCTACGGCTTCGCGATTTTGCACGGGGAAAAGGTGGCCAATCC
GGCTGATGAAGTTGGGCCCGCGTCAATTGCTACTTTCGTGCCGAAGGACCGCTCGCTTTCGCCCTCAGAAATTCGCGTGA
TGCTGAAGCAACTGGAGCACGTTGCCACAATTCCCACTATCCGTTTGGGAATGCGGCTTTATCTGCTCACCATGGTGCGC
AAGAGCGAGCTGCAGGACGCGGTGTGGGACGAGGTCGATTTCGAGAATGCTGTCTGGACGATCCCGAAGGAGCGCATGAA
GCGATCAAAGGCGCACAACGTCTACCTGTCACGCCAAGCGCTCGACATCATGATTGCCCTGAAGACCTGTGCGGGAAATT
CCAGATACCTTCTACCATCGCGGTACGATGCGGACGCCCCGATGTCGCGCGCCACGTTTAATCGGATCACCTACGCAGTC
GTTGAACGGGCGAAGGCGAACGGGCTGCCTCTGGAGCCGTTTACGGTTCATGACCTGCGGCGAACGGGCTCGACCCTGTT
GAATGAGCTCGGCTTCAACAGCGATTGGATCGAGAAATGTCTGGCGCACGAGGACGGGCGCTCGTCGCGAGGTGTCTACA
ATAAGGCTGAGTATGAAGTTCAACGCCGCCACATGATGCAGGAATGGTCGGATATCGTCGACGCCTGGGTCGGTGGCAAG
AAACATGTGCCGACACTCATTCCGCCGTCGATGCCGATCCTGGCGCCCGATCCGGCGCTTTAA

Upstream 100 bases:

>100_bases
GCTCGTGACGGTACTTTTTTGTGCCTTAAGGCATTGAAGAAAAAAGGAATTTCCTGCCACCGGCCCTCGAAATTGTACTG
ACGGTACTTTTCAGGAGGCA

Downstream 100 bases:

>100_bases
CTGGGCGAGCCCATGCTGGCGGAATGCTAGGAGAATTGGCCTGAGCGAGGGCTGGAAGGCCGCGGTGGGTTATAGAGCAG
TCAAAACAGCTCGAATATTG

Product: CP4-like integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MGMLTDVALKALKPKEKIYKIADRDGIYVRVMPSGAISFRLDYRLNGRRETIYLGRYGRDGISLALAREKCLDAKRAIGE
GRSPAIEKQRDKRRLKEAKSFGEFGEKWLTAAPMADSTRAMRRAIFERELLPVWRNRLLTEITPDDLRTHCGAIVERGAP
ATAIHVRDILKQIYGFAILHGEKVANPADEVGPASIATFVPKDRSLSPSEIRVMLKQLEHVATIPTIRLGMRLYLLTMVR
KSELQDAVWDEVDFENAVWTIPKERMKRSKAHNVYLSRQALDIMIALKTCAGNSRYLLPSRYDADAPMSRATFNRITYAV
VERAKANGLPLEPFTVHDLRRTGSTLLNELGFNSDWIEKCLAHEDGRSSRGVYNKAEYEVQRRHMMQEWSDIVDAWVGGK
KHVPTLIPPSMPILAPDPAL

Sequences:

>Translated_420_residues
MGMLTDVALKALKPKEKIYKIADRDGIYVRVMPSGAISFRLDYRLNGRRETIYLGRYGRDGISLALAREKCLDAKRAIGE
GRSPAIEKQRDKRRLKEAKSFGEFGEKWLTAAPMADSTRAMRRAIFERELLPVWRNRLLTEITPDDLRTHCGAIVERGAP
ATAIHVRDILKQIYGFAILHGEKVANPADEVGPASIATFVPKDRSLSPSEIRVMLKQLEHVATIPTIRLGMRLYLLTMVR
KSELQDAVWDEVDFENAVWTIPKERMKRSKAHNVYLSRQALDIMIALKTCAGNSRYLLPSRYDADAPMSRATFNRITYAV
VERAKANGLPLEPFTVHDLRRTGSTLLNELGFNSDWIEKCLAHEDGRSSRGVYNKAEYEVQRRHMMQEWSDIVDAWVGGK
KHVPTLIPPSMPILAPDPAL
>Mature_419_residues
GMLTDVALKALKPKEKIYKIADRDGIYVRVMPSGAISFRLDYRLNGRRETIYLGRYGRDGISLALAREKCLDAKRAIGEG
RSPAIEKQRDKRRLKEAKSFGEFGEKWLTAAPMADSTRAMRRAIFERELLPVWRNRLLTEITPDDLRTHCGAIVERGAPA
TAIHVRDILKQIYGFAILHGEKVANPADEVGPASIATFVPKDRSLSPSEIRVMLKQLEHVATIPTIRLGMRLYLLTMVRK
SELQDAVWDEVDFENAVWTIPKERMKRSKAHNVYLSRQALDIMIALKTCAGNSRYLLPSRYDADAPMSRATFNRITYAVV
ERAKANGLPLEPFTVHDLRRTGSTLLNELGFNSDWIEKCLAHEDGRSSRGVYNKAEYEVQRRHMMQEWSDIVDAWVGGKK
HVPTLIPPSMPILAPDPAL

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=403, Percent_Identity=31.2655086848635, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788974, Length=407, Percent_Identity=31.6953316953317, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788690, Length=394, Percent_Identity=28.1725888324873, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47593; Mature: 47462

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMLTDVALKALKPKEKIYKIADRDGIYVRVMPSGAISFRLDYRLNGRRETIYLGRYGRD
CCCHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCEEEEEEECCCCC
GISLALAREKCLDAKRAIGEGRSPAIEKQRDKRRLKEAKSFGEFGEKWLTAAPMADSTRA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MRRAIFERELLPVWRNRLLTEITPDDLRTHCGAIVERGAPATAIHVRDILKQIYGFAILH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEHCC
GEKVANPADEVGPASIATFVPKDRSLSPSEIRVMLKQLEHVATIPTIRLGMRLYLLTMVR
CCCCCCCHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KSELQDAVWDEVDFENAVWTIPKERMKRSKAHNVYLSRQALDIMIALKTCAGNSRYLLPS
HHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCEEECCC
RYDADAPMSRATFNRITYAVVERAKANGLPLEPFTVHDLRRTGSTLLNELGFNSDWIEKC
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LAHEDGRSSRGVYNKAEYEVQRRHMMQEWSDIVDAWVGGKKHVPTLIPPSMPILAPDPAL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GMLTDVALKALKPKEKIYKIADRDGIYVRVMPSGAISFRLDYRLNGRRETIYLGRYGRD
CCHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCEEEEEEECCCCC
GISLALAREKCLDAKRAIGEGRSPAIEKQRDKRRLKEAKSFGEFGEKWLTAAPMADSTRA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MRRAIFERELLPVWRNRLLTEITPDDLRTHCGAIVERGAPATAIHVRDILKQIYGFAILH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEHCC
GEKVANPADEVGPASIATFVPKDRSLSPSEIRVMLKQLEHVATIPTIRLGMRLYLLTMVR
CCCCCCCHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KSELQDAVWDEVDFENAVWTIPKERMKRSKAHNVYLSRQALDIMIALKTCAGNSRYLLPS
HHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCEEECCC
RYDADAPMSRATFNRITYAVVERAKANGLPLEPFTVHDLRRTGSTLLNELGFNSDWIEKC
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LAHEDGRSSRGVYNKAEYEVQRRHMMQEWSDIVDAWVGGKKHVPTLIPPSMPILAPDPAL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]