Click here to switch to the map view.

The map label for this gene is pncA [C]

Identifier: 13475713

GI number: 13475713

Start: 5642872

End: 5643540

Strand: Direct

Name: pncA [C]

Synonym: mlr6855

Alternate gene names: 13475713

Gene position: 5642872-5643540 (Clockwise)

Preceding gene: 13475712

Following gene: 13475714

Centisome position: 80.2

GC content: 59.79

Gene sequence:

>669_bases
GTGTCCTGGGCAGCAATGGGAGAAGTCCATGAGAAGACACCACCAGCCGGATATTCTTTGATGTCGGTGCCGGGCTTGGC
ATATGGGCCGTTGGGTCACGGCTGCATGCATGTCTGCGTGGATATGCAGCGGCTGTTCGCGGAACCGTCGCCGTGGGCGA
CGCCCTGGATCACGCGTGTCCTCCCTCAGATTAAGAGGCTCGTTGAAAGACGGGCAGAACAGACGGTCTTCACGCGCTTC
CTTCCCGCGCAGAGGGCGGGGCAGGGTGTCGGGGCATGGAAGCGCTACTATGAGCGCTGGTCTTCGATGGCGATCGACAA
TATCGGCCCGGAAATGGTCGAGCTGTTGCCGTCATTGGCTGCTTGCTGTCCGCCGGCGGCGGTCATCGACAAGCATATTT
ATTCGCCCTGGTTTGAAGGCCGTCTGCGAGCCTTTCTGATGGAACACGAGATCGATACGCTGGTCATCACCGGCGGTGAA
ACCGACGTGTGCGTACTGGCCACGGTGCTCGGCGCCATCGATTTCGGCTACCGTGTCGTTCTCGTCACGGACGCGCTGTG
CAGTTCTTCCGATGCAACGCATGACGCCCTGCTCACTGTCTATCACCAACGTTTCGCGCAGCAGGTCGAGACGGTCGAGA
TGGAGACGGTTCTTTCGAACTGGACTTGA

Upstream 100 bases:

>100_bases
GTAGGTCGGGCTGAGCGGCGATCCACAGGCTGAACGCGTCGACCAATCGCGTTGGTTGCCGCCGGTCCACCAGGAACGAG
TCCTGGCTTATGGCGTTCCC

Downstream 100 bases:

>100_bases
ATGTCACCAGGCGTCGAGGAGGCAGAAGCATCAGGTGCTTCTTCGCGCGGGCTGGGATACCTATCTGTCGAGATAAGACA
GAGATCGGAACAGGCAACGG

Product: hypothetical protein

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: Isochorismatase Family Protein; Isochorismatase; Cysteine Hydrolase Family Protein; Hydrolase

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSWAAMGEVHEKTPPAGYSLMSVPGLAYGPLGHGCMHVCVDMQRLFAEPSPWATPWITRVLPQIKRLVERRAEQTVFTRF
LPAQRAGQGVGAWKRYYERWSSMAIDNIGPEMVELLPSLAACCPPAAVIDKHIYSPWFEGRLRAFLMEHEIDTLVITGGE
TDVCVLATVLGAIDFGYRVVLVTDALCSSSDATHDALLTVYHQRFAQQVETVEMETVLSNWT

Sequences:

>Translated_222_residues
MSWAAMGEVHEKTPPAGYSLMSVPGLAYGPLGHGCMHVCVDMQRLFAEPSPWATPWITRVLPQIKRLVERRAEQTVFTRF
LPAQRAGQGVGAWKRYYERWSSMAIDNIGPEMVELLPSLAACCPPAAVIDKHIYSPWFEGRLRAFLMEHEIDTLVITGGE
TDVCVLATVLGAIDFGYRVVLVTDALCSSSDATHDALLTVYHQRFAQQVETVEMETVLSNWT
>Mature_221_residues
SWAAMGEVHEKTPPAGYSLMSVPGLAYGPLGHGCMHVCVDMQRLFAEPSPWATPWITRVLPQIKRLVERRAEQTVFTRFL
PAQRAGQGVGAWKRYYERWSSMAIDNIGPEMVELLPSLAACCPPAAVIDKHIYSPWFEGRLRAFLMEHEIDTLVITGGET
DVCVLATVLGAIDFGYRVVLVTDALCSSSDATHDALLTVYHQRFAQQVETVEMETVLSNWT

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.- [C]

Molecular weight: Translated: 24727; Mature: 24596

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWAAMGEVHEKTPPAGYSLMSVPGLAYGPLGHGCMHVCVDMQRLFAEPSPWATPWITRV
CCCHHHCHHHHCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LPQIKRLVERRAEQTVFTRFLPAQRAGQGVGAWKRYYERWSSMAIDNIGPEMVELLPSLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ACCPPAAVIDKHIYSPWFEGRLRAFLMEHEIDTLVITGGETDVCVLATVLGAIDFGYRVV
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCEEE
LVTDALCSSSDATHDALLTVYHQRFAQQVETVEMETVLSNWT
EEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SWAAMGEVHEKTPPAGYSLMSVPGLAYGPLGHGCMHVCVDMQRLFAEPSPWATPWITRV
CCHHHCHHHHCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LPQIKRLVERRAEQTVFTRFLPAQRAGQGVGAWKRYYERWSSMAIDNIGPEMVELLPSLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ACCPPAAVIDKHIYSPWFEGRLRAFLMEHEIDTLVITGGETDVCVLATVLGAIDFGYRVV
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCEEE
LVTDALCSSSDATHDALLTVYHQRFAQQVETVEMETVLSNWT
EEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA