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The map label for this gene is estB [H]

Identifier: 13475683

GI number: 13475683

Start: 5606779

End: 5607906

Strand: Direct

Name: estB [H]

Synonym: mlr6813

Alternate gene names: 13475683

Gene position: 5606779-5607906 (Clockwise)

Preceding gene: 13475682

Following gene: 13475684

Centisome position: 79.69

GC content: 66.84

Gene sequence:

>1128_bases
ATGAACATGGATGGTATCTGCGATCCGCGCTTTGGTGCCGTGCGCGCGGCATTCGCCGAGTGTTTCGCCCAAGGGCTGGA
ACATGGCGGGGGCGTATCCGTCGTCGTCCACGGCAAGACCGTCGTTGACCTCTGGGGCGGTCATGCCGATGCCGCGCGAA
CCCGTCCGTGGCGGCAGGACACGCTGATCAATGTCTGGTCCTCCACCAAGGGCGTCGTGGCGCTGGCGATCGCAATGTTG
GTCGAGCGCGGCAGGCTGGACTATGCCGCACCCATTGCCCGCTACTGGCCTGAATTCGCGGCCGGCGGCAAGGAACACAT
CACGCTCGACCAGGTGATGTCGCACCAGGCCGGGCTGAATGGTCTTGCCGTGCCGATGGACGAAGCGGGCCTGTTCGCGT
GGACGCCTTTTGTGGACGCGCTTGCGGCCATGCCGCCACTCTGGGAGCCCGGCAGCCGCTGCATCTATCACGCCCTTACC
TACGGCCACCTTGCTGGCGAAGTGCTGCGCCGCGTCGACGGGCGAAGCATCGGCCGCTTCATCGGCGAAGAGATCGCGGG
CCCGCTTGGAGCTGACTTTCATGTCGGTCTGCCGGATCGTGAGGATTCCAGGGTCGCCGAGATGATCGAAGGCCCCGGCG
CTTCGGACTGGGTCGATTTTGTCCGCGCCTCGCCCTTTCCCCACGCAAGCGACAATCCGGCGCCCCGTGCATTGGCCCCC
AACGACCGCGTCTGGCGCGCCGCCGAGGTGCCGGGCGGCAACGGCCAGTCGACGGCGCATGCACTGGCCCGTATTTACGG
CATGATGGCAGCCGGTGGCGTCTGGGAGGGCCATCCACTGATCGGGCGCGCGGCAATCGAAGCGGCGGCGCGTCAGCGTG
TTCGCGGCACGGATGAAAGCTTCGCCGCACCGGCCGCCTTCGCCGCCGGCTATCAGATGGAGGATCCGGTCTATGCCGGC
CGCGCGTCGCCGCAAACCTTCGGCCACACCGGCTGGGGCGGCGCCATCGGCTTTGCCGATCCCGGTGCCGGCATCGGTTT
CGGCTACGTCACCAACCGCATGCTGGGTTTTGACGACATGGACCCACGCCGCAAGATGTTGATCGACGCCGTCTACGACT
CGCTCTGA

Upstream 100 bases:

>100_bases
GATCTTGAATGTCGGCAATTCGCTGGACTGCTGGGTTCCCGCCGAGCCAGAATGGTCGACGGCGGCATTCCCGTCATATC
AGCCTGGTGAGAGTTCACGC

Downstream 100 bases:

>100_bases
CTGAATGGCCTAAAAGCCAGCCGGGTGGTTCCGCAGGCTGGAAACGGTGCGCGCCACTGCCGAGCTCCGCAACGGACAGC
GTTCGCACGTGCGTTCGGCT

Product: esterase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNMDGICDPRFGAVRAAFAECFAQGLEHGGGVSVVVHGKTVVDLWGGHADAARTRPWRQDTLINVWSSTKGVVALAIAML
VERGRLDYAAPIARYWPEFAAGGKEHITLDQVMSHQAGLNGLAVPMDEAGLFAWTPFVDALAAMPPLWEPGSRCIYHALT
YGHLAGEVLRRVDGRSIGRFIGEEIAGPLGADFHVGLPDREDSRVAEMIEGPGASDWVDFVRASPFPHASDNPAPRALAP
NDRVWRAAEVPGGNGQSTAHALARIYGMMAAGGVWEGHPLIGRAAIEAAARQRVRGTDESFAAPAAFAAGYQMEDPVYAG
RASPQTFGHTGWGGAIGFADPGAGIGFGYVTNRMLGFDDMDPRRKMLIDAVYDSL

Sequences:

>Translated_375_residues
MNMDGICDPRFGAVRAAFAECFAQGLEHGGGVSVVVHGKTVVDLWGGHADAARTRPWRQDTLINVWSSTKGVVALAIAML
VERGRLDYAAPIARYWPEFAAGGKEHITLDQVMSHQAGLNGLAVPMDEAGLFAWTPFVDALAAMPPLWEPGSRCIYHALT
YGHLAGEVLRRVDGRSIGRFIGEEIAGPLGADFHVGLPDREDSRVAEMIEGPGASDWVDFVRASPFPHASDNPAPRALAP
NDRVWRAAEVPGGNGQSTAHALARIYGMMAAGGVWEGHPLIGRAAIEAAARQRVRGTDESFAAPAAFAAGYQMEDPVYAG
RASPQTFGHTGWGGAIGFADPGAGIGFGYVTNRMLGFDDMDPRRKMLIDAVYDSL
>Mature_375_residues
MNMDGICDPRFGAVRAAFAECFAQGLEHGGGVSVVVHGKTVVDLWGGHADAARTRPWRQDTLINVWSSTKGVVALAIAML
VERGRLDYAAPIARYWPEFAAGGKEHITLDQVMSHQAGLNGLAVPMDEAGLFAWTPFVDALAAMPPLWEPGSRCIYHALT
YGHLAGEVLRRVDGRSIGRFIGEEIAGPLGADFHVGLPDREDSRVAEMIEGPGASDWVDFVRASPFPHASDNPAPRALAP
NDRVWRAAEVPGGNGQSTAHALARIYGMMAAGGVWEGHPLIGRAAIEAAARQRVRGTDESFAAPAAFAAGYQMEDPVYAG
RASPQTFGHTGWGGAIGFADPGAGIGFGYVTNRMLGFDDMDPRRKMLIDAVYDSL

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538001, Length=400, Percent_Identity=32.5, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71983659, Length=395, Percent_Identity=29.620253164557, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17535191, Length=400, Percent_Identity=31.25, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71983666, Length=304, Percent_Identity=32.2368421052632, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17535177, Length=399, Percent_Identity=28.5714285714286, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17538474, Length=400, Percent_Identity=28.25, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71990590, Length=395, Percent_Identity=25.3164556962025, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 39990; Mature: 39990

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMDGICDPRFGAVRAAFAECFAQGLEHGGGVSVVVHGKTVVDLWGGHADAARTRPWRQD
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCHHHCCCCCCC
TLINVWSSTKGVVALAIAMLVERGRLDYAAPIARYWPEFAAGGKEHITLDQVMSHQAGLN
HHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCEEHHHHHHHHCCCC
GLAVPMDEAGLFAWTPFVDALAAMPPLWEPGSRCIYHALTYGHLAGEVLRRVDGRSIGRF
CCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IGEEIAGPLGADFHVGLPDREDSRVAEMIEGPGASDWVDFVRASPFPHASDNPAPRALAP
HHHHHCCCCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
NDRVWRAAEVPGGNGQSTAHALARIYGMMAAGGVWEGHPLIGRAAIEAAARQRVRGTDES
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
FAAPAAFAAGYQMEDPVYAGRASPQTFGHTGWGGAIGFADPGAGIGFGYVTNRMLGFDDM
HCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DPRRKMLIDAVYDSL
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MNMDGICDPRFGAVRAAFAECFAQGLEHGGGVSVVVHGKTVVDLWGGHADAARTRPWRQD
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCHHHCCCCCCC
TLINVWSSTKGVVALAIAMLVERGRLDYAAPIARYWPEFAAGGKEHITLDQVMSHQAGLN
HHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCEEHHHHHHHHCCCC
GLAVPMDEAGLFAWTPFVDALAAMPPLWEPGSRCIYHALTYGHLAGEVLRRVDGRSIGRF
CCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IGEEIAGPLGADFHVGLPDREDSRVAEMIEGPGASDWVDFVRASPFPHASDNPAPRALAP
HHHHHCCCCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
NDRVWRAAEVPGGNGQSTAHALARIYGMMAAGGVWEGHPLIGRAAIEAAARQRVRGTDES
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
FAAPAAFAAGYQMEDPVYAGRASPQTFGHTGWGGAIGFADPGAGIGFGYVTNRMLGFDDM
HCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DPRRKMLIDAVYDSL
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]