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The map label for this gene is pno [H]

Identifier: 13475661

GI number: 13475661

Start: 5584312

End: 5585874

Strand: Reverse

Name: pno [H]

Synonym: mll6785

Alternate gene names: 13475661

Gene position: 5585874-5584312 (Counterclockwise)

Preceding gene: 13475662

Following gene: 13475659

Centisome position: 79.39

GC content: 64.94

Gene sequence:

>1563_bases
GTGGCAGATGGAGTGAGGATGACCAGGGCCAAGGTTGAGCACGCACCGAATTGCGATATCGTCATCGTCGGCGGCGGGTC
GGCCGGGTCGTTGCTTGCCGCGCGGCTGAGCGAAGATCCGGACAGCCGCGTTCTGCTGATCGAGGCAGGCGAGGAGCCGA
CGGATCCCGATATCTGGAACCCCGCCGCATGGCCGGCGCTACAGGGCCGCAGCTACGATTGGGACTACCGCACGGAGGCG
CAAGCCGGCACCGCGGGCCGGGCGCATCATTGGGCGCGCGGGCGGTTGATCGGCGGCTCGAGTTGCCTGCACGCGATGGG
CTACATGCGCGGTCATCCCTCCGACTTCCAGGCCTGGGTCGATGCGTCAGGGGATCGCCGATGGGGTTGGGATGAACTGC
TGCCGGTTTTCCAGGCCATCGAAGACCATCCACTTGGTGGCGACGGCATTCACGGCAAGGGCGGACCGTTGCCGATCCAT
TTGCCGGCGGACGAAGTCAGTCCGCTTGCTCGTGCCTTCATCGAGGCAGGCGCATCGTTGGGTCTGCCTCGCCTTGAAGG
CCACAACAGCGGAGAGATGATCGGCGTCACCCCGAATTCCTTGAACATTCGCGACGGGCGAAGGGTTACGGCGGCGGACG
CCTGGCTCACAAAGGCGGTTCGCGGCCGCAAGAACCTGACCATCCTTACCGGCTCCCGGGTGCGGCGACTCAAGCTGGAG
GGCAATCAGGTCCGCAGCCTCGAAGTCGTGGGGCGACAGGGTTCGGCCGAAGTGTTTGCGGATCAAATCGTGCTTTGTGC
TGGAGCGCTGGAAAGTCCGGCCTTGCTGATGCGTTCGGGCATCGGTCCGCACGATGTGCTCGATGCGGCGGGCGTCGGCT
GCCTGATCGACATGCCTGACATCGGTCGAAACCTTCAGGACCACCTGCTTGGCGCCGGCAACCTTTACGCGGCCCGCAAG
CCGGTGCCGCCATCGCGCCTCCAACATTCGGAATCGATGGCCTATATGCGGGCTGACAGCTTCACCGCCGCCGGGCAGCC
TGAAATTGTCGTTGGCTGCGGTGTCGCGCCGATCGTGTCGGAAAGCTTCCCGGCCCCCGCCGCCGGCTCGGCCTATTCGC
TGCTGTTCGGGATCACCCATCCGACGAGCCGCGGCAGCGTGCGCATAAGTGGGCCTGAGCTCGGCGATCGGCTGATCATC
GACCCGGCATACCTGCAAACCGGCCGGGACCGCGAGCGGTTTCGCAGAGCACTCGAGGCATCTCGGACCATCGGGCACAG
AGATGAACTCGCCGGCTGGCGAGAGCGCGAACTCCTGCCAGGCACGCCGAACAGTGCGGCCGAGATGGACGACTTCATCG
CGCGATCGGTCATCACACACCATCATCCCTGCGGTACTTGCCGGATGGGCAAGGACCCGGACGCCGTCGTCGATGCAAAT
CTACGGCTCAAGGCGCTCGACAATCTTTTTGTCGTGGACGCGTCGATCATGCCCAACCTCACCGCGGGACCGATTCATGC
CGCGGTCCTCGCGATCGCCGAGACTTTCGCCCGACAGTACTAA

Upstream 100 bases:

>100_bases
ACTCGAAGTCCTTCATGACGTTTCGGATCGGCCGAACGCGGATCCGTGCGCGGGCCTTCTCCGTGTCGGTGTCAGACATG
ACGGCTATCGCAACCTGCAA

Downstream 100 bases:

>100_bases
GCCACCGACGCCGTCGGCTCGGCTTGCAGTACGGCGGGCCGGTGCGCCGCCTTGGCATATTTCTGGGCGATCATGGCACA
GACCATGAGCTGTATCTGGT

Product: dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MADGVRMTRAKVEHAPNCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWNPAAWPALQGRSYDWDYRTEA
QAGTAGRAHHWARGRLIGGSSCLHAMGYMRGHPSDFQAWVDASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIH
LPADEVSPLARAFIEAGASLGLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLE
GNQVRSLEVVGRQGSAEVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVGCLIDMPDIGRNLQDHLLGAGNLYAARK
PVPPSRLQHSESMAYMRADSFTAAGQPEIVVGCGVAPIVSESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLII
DPAYLQTGRDRERFRRALEASRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDAN
LRLKALDNLFVVDASIMPNLTAGPIHAAVLAIAETFARQY

Sequences:

>Translated_520_residues
MADGVRMTRAKVEHAPNCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWNPAAWPALQGRSYDWDYRTEA
QAGTAGRAHHWARGRLIGGSSCLHAMGYMRGHPSDFQAWVDASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIH
LPADEVSPLARAFIEAGASLGLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLE
GNQVRSLEVVGRQGSAEVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVGCLIDMPDIGRNLQDHLLGAGNLYAARK
PVPPSRLQHSESMAYMRADSFTAAGQPEIVVGCGVAPIVSESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLII
DPAYLQTGRDRERFRRALEASRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDAN
LRLKALDNLFVVDASIMPNLTAGPIHAAVLAIAETFARQY
>Mature_519_residues
ADGVRMTRAKVEHAPNCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWNPAAWPALQGRSYDWDYRTEAQ
AGTAGRAHHWARGRLIGGSSCLHAMGYMRGHPSDFQAWVDASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIHL
PADEVSPLARAFIEAGASLGLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLEG
NQVRSLEVVGRQGSAEVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVGCLIDMPDIGRNLQDHLLGAGNLYAARKP
VPPSRLQHSESMAYMRADSFTAAGQPEIVVGCGVAPIVSESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLIID
PAYLQTGRDRERFRRALEASRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDANL
RLKALDNLFVVDASIMPNLTAGPIHAAVLAIAETFARQY

Specific function: Can Catalyze The Oxidation Of Choline To Betaine Aldehyde And Betaine Aldehyde To Glycine Betaine At The Same Rate. [C]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=530, Percent_Identity=35.8490566037736, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1786503, Length=535, Percent_Identity=31.7757009345794, Blast_Score=242, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17532301, Length=545, Percent_Identity=30.2752293577982, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24642042, Length=560, Percent_Identity=30.7142857142857, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI18859993, Length=564, Percent_Identity=30.4964539007092, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24642051, Length=556, Percent_Identity=29.136690647482, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24650267, Length=561, Percent_Identity=30.4812834224599, Blast_Score=195, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24642048, Length=559, Percent_Identity=30.5903398926655, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17137792, Length=558, Percent_Identity=29.3906810035842, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24642059, Length=560, Percent_Identity=31.0714285714286, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24642037, Length=565, Percent_Identity=30.2654867256637, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI18859995, Length=558, Percent_Identity=29.5698924731183, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45551458, Length=558, Percent_Identity=29.0322580645161, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45549471, Length=559, Percent_Identity=29.5169946332737, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24642035, Length=565, Percent_Identity=29.3805309734513, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24642055, Length=314, Percent_Identity=35.3503184713376, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24642039, Length=558, Percent_Identity=28.1362007168459, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24642057, Length=576, Percent_Identity=26.7361111111111, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: =1.1.3.12 [H]

Molecular weight: Translated: 55713; Mature: 55582

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00626 RCC1_2 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGVRMTRAKVEHAPNCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWN
CCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
PAAWPALQGRSYDWDYRTEAQAGTAGRAHHWARGRLIGGSSCLHAMGYMRGHPSDFQAWV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHCCCCCHHHHHC
DASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIHLPADEVSPLARAFIEAGASL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHC
GLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLE
CCCCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEECCCEEEEEEC
GNQVRSLEVVGRQGSAEVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVGCLIDMPD
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCEEEEECCH
IGRNLQDHLLGAGNLYAARKPVPPSRLQHSESMAYMRADSFTAAGQPEIVVGCGVAPIVS
HCCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCHHHHH
ESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLIIDPAYLQTGRDRERFRRALEA
CCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCEEEECHHHHHCCCCHHHHHHHHHH
SRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDAN
HHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC
LRLKALDNLFVVDASIMPNLTAGPIHAAVLAIAETFARQY
EEEEEECCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADGVRMTRAKVEHAPNCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWN
CCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
PAAWPALQGRSYDWDYRTEAQAGTAGRAHHWARGRLIGGSSCLHAMGYMRGHPSDFQAWV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHCCCCCHHHHHC
DASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIHLPADEVSPLARAFIEAGASL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHC
GLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLE
CCCCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEECCCEEEEEEC
GNQVRSLEVVGRQGSAEVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVGCLIDMPD
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCEEEEECCH
IGRNLQDHLLGAGNLYAARKPVPPSRLQHSESMAYMRADSFTAAGQPEIVVGCGVAPIVS
HCCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCHHHHH
ESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLIIDPAYLQTGRDRERFRRALEA
CCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCEEEECHHHHHCCCCHHHHHHHHHH
SRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDAN
HHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC
LRLKALDNLFVVDASIMPNLTAGPIHAAVLAIAETFARQY
EEEEEECCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12092811 [H]