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The map label for this gene is ilvB [H]

Identifier: 13475549

GI number: 13475549

Start: 5444877

End: 5446679

Strand: Direct

Name: ilvB [H]

Synonym: mlr6648

Alternate gene names: 13475549

Gene position: 5444877-5446679 (Clockwise)

Preceding gene: 13475547

Following gene: 229259632

Centisome position: 77.39

GC content: 62.9

Gene sequence:

>1803_bases
ATGAAGCTGACGGGCGGTCAAATCGTTGCAAGGGCACTCAAGGAGTATGGCGTCGAGTACGTGGCTGGCGTTCCCGGCCA
CGGCATCTGGTCGCTGTTTGACGCCTTCCTGGAAGAGGGGTCGACCCTTCCATTCATCCAGGTCATGCATGAGCAGAGCG
CCGTGCATATGGCCGATGGCTTCTTCCGGGCCAGCGGTCGGCCGATGGCCTGCTCCACATCGATCGGTCCTGGCGCGACG
AACACGATCATCGGACTTGCGACCTGCTACGCCGACTCCATTCCCGCCTTTTTTGTCTCCGGCGGCCCGGCGACCCACAT
GAAGGGTCACGGCGTGATGCAGGAGATCGAGCGGCAGAACGAGAACGCTTTCCCTCGCATCACCGAACAGGTCACCAAGC
GCGCCTACAAGGCTGGCCGCGTCGACGAACTGCCCTTCATCATGCACCGCGCCTTCAACACCATGCTGAGCGGCCGTCCC
GGACCCGTCCATGTCGAGGTCCCGATGGACATCCAGGTCGAGGCGGCCGATGTCGAGATTCATCCACTCGATCTTCGCAT
GGCGCGCGGCGTTTCCTACCCGGATCCGAAAGCGGTCGAGCGGGCCGTGAAGCTGCTGCTGTCGGCCGAACGGCCGGTCA
TCGTCGCAGGCGGCGGTGCGATTTCCGCCAATGCGTCGGGAGAGCTCACGAGGCTCGCCGAAAAGCTGGGTGCCGCGGTT
TCCATTACCTGGAACGGCAAGGGCGCCATTTCCGAAGACCATGCGCTGTTCATCGGCGCTGTCGGCCAGACCGGCACGAC
CTGCGGCAACAGCATTACTGCATCGGCCGATGTCATCATGTCGGTCGGCTGCCGCTTCACCGACTGGTCGGCTTCCTCCT
ACGCCAAGGGCGTCAGCTTTTCGATTCCGCCGGGCAAGCTCATCCACATCGATCTCGACCACCACGAGATCGGCAAGAAC
TATCCGACGGAGGTTGGCATCGTGGCCGATGCCAAGGCAACGCTAGAAGCGATGCTGGCCATGATTTCGGAGGCCGAGTC
GAAGAAAGCGGTCATGCGCCGCGACAGGTTTCTGGCCGATGTCCAGAAAGCGAAGGCAGACTGGGAAGCGCTGCTTGCGC
CGCGCCGCGATAGCCGCGAGTCGCCCTTCACGTCGCAGCGGCCGCTTGGTGCGTTACGCACGGTCATGAGTCGCGACGGC
ATCGTCGTTGTCGGGTCGGGCAACACGCAAGGCGCGGTCAAGCAGACCTTTCCGATCTACCAGCCGCGGACCCACCTCAC
CTCCGGCTCATTCTCGCCGATGGGCTGGGCCGTTCCCGCCGCAATGGGCGCCAAGCTGGCAATGCCGGACCGTCAGGTCG
TATCCATCACCGGCGACGGCGATTTCATGATGTCGCTGCCCGAACTCGGTACCGCCGTGATGAACAACATTCCTGTCGTG
TTCCTGGTCCAGAACAACCAGGGCTACATGTCGATCCGTGGCGGTCAGCGCAAGTTCATGGGACGACACATCGCCTCCGA
ATTCAACCGCCACAAGGGTAATGGCGAACCCTACAGCGCCGACATTGCGGCGGTCGCGAGGAACTTCGGCGTCGAGAGCT
GGAAGGTCACGGCCGACGAGGATCTGGAGAAATCGCTGAAGGCCGCTCTCGATTGTGGCGGCCCGGCGCTGGTCGAGGTC
ATAACCTCTCGCGATGCGGCCGGTCCGTTTGCCACCGGCTGGTGGGATTTTCCATCGCCCGCCTACTACGAGAAAGAGCA
GGCGGCTTACACCGAGAAGCGCGTGCTCGAACAGCACATGTAG

Upstream 100 bases:

>100_bases
ATTGTCTGCCTACTTGCGCTATCACCGAGGGCCGGCTTTCAGGGACCCTCATCTTGAGCCCGCTTGAGGCAAAAGAGCAA
AAATCAGAGGGTCGATAGAG

Downstream 100 bases:

>100_bases
CCGTACCTGGGGCGAGCAGCCCTGGCTGCTCGCGCTTCATTCCATTGATTCAGACCGCAATGCGGGGCGTAGGCCCTGCA
AACGAAGAGCCATTGCCATG

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKLTGGQIVARALKEYGVEYVAGVPGHGIWSLFDAFLEEGSTLPFIQVMHEQSAVHMADGFFRASGRPMACSTSIGPGAT
NTIIGLATCYADSIPAFFVSGGPATHMKGHGVMQEIERQNENAFPRITEQVTKRAYKAGRVDELPFIMHRAFNTMLSGRP
GPVHVEVPMDIQVEAADVEIHPLDLRMARGVSYPDPKAVERAVKLLLSAERPVIVAGGGAISANASGELTRLAEKLGAAV
SITWNGKGAISEDHALFIGAVGQTGTTCGNSITASADVIMSVGCRFTDWSASSYAKGVSFSIPPGKLIHIDLDHHEIGKN
YPTEVGIVADAKATLEAMLAMISEAESKKAVMRRDRFLADVQKAKADWEALLAPRRDSRESPFTSQRPLGALRTVMSRDG
IVVVGSGNTQGAVKQTFPIYQPRTHLTSGSFSPMGWAVPAAMGAKLAMPDRQVVSITGDGDFMMSLPELGTAVMNNIPVV
FLVQNNQGYMSIRGGQRKFMGRHIASEFNRHKGNGEPYSADIAAVARNFGVESWKVTADEDLEKSLKAALDCGGPALVEV
ITSRDAAGPFATGWWDFPSPAYYEKEQAAYTEKRVLEQHM

Sequences:

>Translated_600_residues
MKLTGGQIVARALKEYGVEYVAGVPGHGIWSLFDAFLEEGSTLPFIQVMHEQSAVHMADGFFRASGRPMACSTSIGPGAT
NTIIGLATCYADSIPAFFVSGGPATHMKGHGVMQEIERQNENAFPRITEQVTKRAYKAGRVDELPFIMHRAFNTMLSGRP
GPVHVEVPMDIQVEAADVEIHPLDLRMARGVSYPDPKAVERAVKLLLSAERPVIVAGGGAISANASGELTRLAEKLGAAV
SITWNGKGAISEDHALFIGAVGQTGTTCGNSITASADVIMSVGCRFTDWSASSYAKGVSFSIPPGKLIHIDLDHHEIGKN
YPTEVGIVADAKATLEAMLAMISEAESKKAVMRRDRFLADVQKAKADWEALLAPRRDSRESPFTSQRPLGALRTVMSRDG
IVVVGSGNTQGAVKQTFPIYQPRTHLTSGSFSPMGWAVPAAMGAKLAMPDRQVVSITGDGDFMMSLPELGTAVMNNIPVV
FLVQNNQGYMSIRGGQRKFMGRHIASEFNRHKGNGEPYSADIAAVARNFGVESWKVTADEDLEKSLKAALDCGGPALVEV
ITSRDAAGPFATGWWDFPSPAYYEKEQAAYTEKRVLEQHM
>Mature_600_residues
MKLTGGQIVARALKEYGVEYVAGVPGHGIWSLFDAFLEEGSTLPFIQVMHEQSAVHMADGFFRASGRPMACSTSIGPGAT
NTIIGLATCYADSIPAFFVSGGPATHMKGHGVMQEIERQNENAFPRITEQVTKRAYKAGRVDELPFIMHRAFNTMLSGRP
GPVHVEVPMDIQVEAADVEIHPLDLRMARGVSYPDPKAVERAVKLLLSAERPVIVAGGGAISANASGELTRLAEKLGAAV
SITWNGKGAISEDHALFIGAVGQTGTTCGNSITASADVIMSVGCRFTDWSASSYAKGVSFSIPPGKLIHIDLDHHEIGKN
YPTEVGIVADAKATLEAMLAMISEAESKKAVMRRDRFLADVQKAKADWEALLAPRRDSRESPFTSQRPLGALRTVMSRDG
IVVVGSGNTQGAVKQTFPIYQPRTHLTSGSFSPMGWAVPAAMGAKLAMPDRQVVSITGDGDFMMSLPELGTAVMNNIPVV
FLVQNNQGYMSIRGGQRKFMGRHIASEFNRHKGNGEPYSADIAAVARNFGVESWKVTADEDLEKSLKAALDCGGPALVEV
ITSRDAAGPFATGWWDFPSPAYYEKEQAAYTEKRVLEQHM

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=589, Percent_Identity=24.6179966044143, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI21361361, Length=554, Percent_Identity=24.7292418772563, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=31.1030741410488, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=30, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=32.2751322751323, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=23.8938053097345, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788716, Length=576, Percent_Identity=25.1736111111111, Blast_Score=138, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=499, Percent_Identity=25.6513026052104, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=499, Percent_Identity=25.6513026052104, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=601, Percent_Identity=23.2945091514143, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=29.0940766550523, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=502, Percent_Identity=23.5059760956175, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=579, Percent_Identity=23.1433506044905, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323073, Length=508, Percent_Identity=22.8346456692913, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=516, Percent_Identity=22.8682170542636, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=27.0650263620387, Blast_Score=155, Evalue=1e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64528; Mature: 64528

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTGGQIVARALKEYGVEYVAGVPGHGIWSLFDAFLEEGSTLPFIQVMHEQSAVHMADG
CCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHCC
FFRASGRPMACSTSIGPGATNTIIGLATCYADSIPAFFVSGGPATHMKGHGVMQEIERQN
EEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCC
ENAFPRITEQVTKRAYKAGRVDELPFIMHRAFNTMLSGRPGPVHVEVPMDIQVEAADVEI
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCEEE
HPLDLRMARGVSYPDPKAVERAVKLLLSAERPVIVAGGGAISANASGELTRLAEKLGAAV
EEECHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCHHHHHHHHHCCEE
SITWNGKGAISEDHALFIGAVGQTGTTCGNSITASADVIMSVGCRFTDWSASSYAKGVSF
EEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHCCCEE
SIPPGKLIHIDLDHHEIGKNYPTEVGIVADAKATLEAMLAMISEAESKKAVMRRDRFLAD
ECCCCCEEEEECCHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQKAKADWEALLAPRRDSRESPFTSQRPLGALRTVMSRDGIVVVGSGNTQGAVKQTFPIY
HHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCC
QPRTHLTSGSFSPMGWAVPAAMGAKLAMPDRQVVSITGDGDFMMSLPELGTAVMNNIPVV
CCCCCCCCCCCCCCCCHHHHHHCCEEECCCCEEEEEECCCCEEEECHHHHHHHHCCCCEE
FLVQNNQGYMSIRGGQRKFMGRHIASEFNRHKGNGEPYSADIAAVARNFGVESWKVTADE
EEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCH
DLEKSLKAALDCGGPALVEVITSRDAAGPFATGWWDFPSPAYYEKEQAAYTEKRVLEQHM
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLTGGQIVARALKEYGVEYVAGVPGHGIWSLFDAFLEEGSTLPFIQVMHEQSAVHMADG
CCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHCC
FFRASGRPMACSTSIGPGATNTIIGLATCYADSIPAFFVSGGPATHMKGHGVMQEIERQN
EEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCC
ENAFPRITEQVTKRAYKAGRVDELPFIMHRAFNTMLSGRPGPVHVEVPMDIQVEAADVEI
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCEEE
HPLDLRMARGVSYPDPKAVERAVKLLLSAERPVIVAGGGAISANASGELTRLAEKLGAAV
EEECHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCHHHHHHHHHCCEE
SITWNGKGAISEDHALFIGAVGQTGTTCGNSITASADVIMSVGCRFTDWSASSYAKGVSF
EEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHCCCEE
SIPPGKLIHIDLDHHEIGKNYPTEVGIVADAKATLEAMLAMISEAESKKAVMRRDRFLAD
ECCCCCEEEEECCHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQKAKADWEALLAPRRDSRESPFTSQRPLGALRTVMSRDGIVVVGSGNTQGAVKQTFPIY
HHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCC
QPRTHLTSGSFSPMGWAVPAAMGAKLAMPDRQVVSITGDGDFMMSLPELGTAVMNNIPVV
CCCCCCCCCCCCCCCCHHHHHHCCEEECCCCEEEEEECCCCEEEECHHHHHHHHCCCCEE
FLVQNNQGYMSIRGGQRKFMGRHIASEFNRHKGNGEPYSADIAAVARNFGVESWKVTADE
EEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCH
DLEKSLKAALDCGGPALVEVITSRDAAGPFATGWWDFPSPAYYEKEQAAYTEKRVLEQHM
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]