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The map label for this gene is acsA1 [H]

Identifier: 13475461

GI number: 13475461

Start: 5343726

End: 5345684

Strand: Direct

Name: acsA1 [H]

Synonym: mlr6539

Alternate gene names: 13475461

Gene position: 5343726-5345684 (Clockwise)

Preceding gene: 13475459

Following gene: 13475463

Centisome position: 75.95

GC content: 63.65

Gene sequence:

>1959_bases
ATGGCTGCCGAAGTACCGCTCTGGACCCCGACGCAAGACCGGATCGACGCTTCGCCATTGACGGCTTTCCTGAAGGCCGC
GGAGGCGAAAGCCGGCGCCACATTCGCCTCTTATGCCGATCTTCACCGCTGGTCGGTCGAGGATCGCGAGGGGTTCTGGA
GCCTGGTCTGGGATTTCTGCGGCATCGCCGGCGAAAAGGGCGAGCGCCTGCTCGCCGATGGCGACAAGATGCCGGGTGCC
TCCTTCTTTCCCGACGCGACGTTGAATTTCGCCGAGAACCTGTTGAAGAAGACCGGCGCGGGCGAGGCGATCGTCTTCCG
CGGCGAGGACAAGGTCGAGCGGCGGCTGTCCTGGAACCAACTGCATGGCCTGGTTTCGCGCCTGCAGCAGCTTTTCCTGT
CGCTCAAGGTCAAGAAGGGCGACCGCATTGCCGCGATGATGCCCAACATGCCCGAGACAGTCGCTGCCATGCTGGCCGCC
GCCTCCATCGGCGCGGTGTGGTCATCCTGCTCGCCCGACTTCGGCGAGCAGGGCGTGCTCGACCGGTTCGGCCAGATCGA
GCCCGTCATCTTCATCGGGCCTGACGGCTATTGGTACAATGGCAAGGCGATCGAGGTCGCTGACAAGGTCGCCGCGGTCG
CGGCCAAGCTCGGCAGTGTCCGCAAGGTGCTGCTTGTCGATTATCTCGGTACTTCGGCCGATGTGGCGGCAACCATCGAC
AAGGCGGTGGCGATGGAAGAGGCGCTGTCGCCCTTCGCCGCCAAGCCAGTCAGCTTCGAGCCTCTGCCGTTTTCGCATCC
GCTCTACATCCTGTTTTCATCAGGAACCACCGGCATCCCCAAATGCATCGTCCATTCGGCCGGCGGGACGCTGATCCAGC
ATGTCAAGGAACACCGGCTGCATGCCGGCCTCGTCGACGGCGACCGCTTCTTCTACTTCACCACGTGCGGCTGGATGATG
TGGAACTGGCTGGTGTCGGGCCTGGCTTCCGGGGCGACGCTGCTGCTCTACGACGGCTCGCCCTTCTACCCCGACGGCAA
TGTGCTGTTCGACTTCGTCGATGCCGAGAAGATGACCTTCTTCGGCACCTCGGCCAAGTTCATCGATTCCGTGCGCAAGG
CCGGCCTCAAGCCGATCCGCAGCCATGACCTGTCGACCGTGCGGGCGATCTCCTCCACCGGTTCGCCCCTGTCGCCCGAG
GATTTCCGCTTCGTCTACGAAGGCATCAAGAAGGATGTGCATCTCGCCTCGGTTTCCGGCGGCACCGACATCGTCTCCTG
CTTCGTGCTCGGCGTGCCCAACCAGCCGGTGTGGACCGGCGAGATCCAGGGACCAGGCCTCGGCCTGGCCGTCGATGTCT
GGGACGATGACGGCAAGCCGATCCGGCAGGAAAAGGGCGAACTGGTCTGCACCAAGGCGTTTCCGTCGATGCCGATCGGC
TTCTGGAACGATCCCGAAGGCAAGAAATACCAGGCGGCCTATTTCGAGCGTTTCGACAATGTCTGGTGCCATGGCGATTT
CGCTGAGTGGACGGCGCATGGCGGCATGATCATCCATGGCCGCTCGGACGCCACCCTCAATCCGGGCGGGGTGCGCATCG
GCACGGCGGAGATCTACAACCAGGTCGAACAGATGCCGGAAATCCTGGAGGCGCTGTGCATCGGCCAGGATTTCGACAAT
GACGTGCGTGTCGTGCTGTTCGTGCGGCTGGCCGCGGGCGTCGAACTGGACGAGGATCTGGAAAAACGCATCCGCGCCAA
GATCCGCAGCGGTGCCAGCCCCCGCCATGTCCCGGCCAGGATCGTCGCGGTCACGGATATTCCGCGCACCAAGTCGGGCA
AGATCACCGAACTCGCCGTGCGCGACGTCGTGCATGGCCGCGCCATCAAGAACAAGGAAGCGCTCGCCAACCCGGAAGCG
CTGGAGCTGTTCAGGAACCTGCCGCAGCTTGCCGAATGA

Upstream 100 bases:

>100_bases
GCGATGGCATTTGCGCACACCTGGCCGCTTCGGCCCAACAAAATCTTGCTCTGCTGCGCTGCGATATGCATAAGCGATGA
CGACCGTGGGAGGATCGATA

Downstream 100 bases:

>100_bases
CCCCGATCGGCATGGTTAACGAACTATGTTGCGGGAATTTACCTAGATTTCACGAGTGGTACACATTCGTAAATTTTTCG
GCGGTCCGGTAAGGACTTGT

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MAAEVPLWTPTQDRIDASPLTAFLKAAEAKAGATFASYADLHRWSVEDREGFWSLVWDFCGIAGEKGERLLADGDKMPGA
SFFPDATLNFAENLLKKTGAGEAIVFRGEDKVERRLSWNQLHGLVSRLQQLFLSLKVKKGDRIAAMMPNMPETVAAMLAA
ASIGAVWSSCSPDFGEQGVLDRFGQIEPVIFIGPDGYWYNGKAIEVADKVAAVAAKLGSVRKVLLVDYLGTSADVAATID
KAVAMEEALSPFAAKPVSFEPLPFSHPLYILFSSGTTGIPKCIVHSAGGTLIQHVKEHRLHAGLVDGDRFFYFTTCGWMM
WNWLVSGLASGATLLLYDGSPFYPDGNVLFDFVDAEKMTFFGTSAKFIDSVRKAGLKPIRSHDLSTVRAISSTGSPLSPE
DFRFVYEGIKKDVHLASVSGGTDIVSCFVLGVPNQPVWTGEIQGPGLGLAVDVWDDDGKPIRQEKGELVCTKAFPSMPIG
FWNDPEGKKYQAAYFERFDNVWCHGDFAEWTAHGGMIIHGRSDATLNPGGVRIGTAEIYNQVEQMPEILEALCIGQDFDN
DVRVVLFVRLAAGVELDEDLEKRIRAKIRSGASPRHVPARIVAVTDIPRTKSGKITELAVRDVVHGRAIKNKEALANPEA
LELFRNLPQLAE

Sequences:

>Translated_652_residues
MAAEVPLWTPTQDRIDASPLTAFLKAAEAKAGATFASYADLHRWSVEDREGFWSLVWDFCGIAGEKGERLLADGDKMPGA
SFFPDATLNFAENLLKKTGAGEAIVFRGEDKVERRLSWNQLHGLVSRLQQLFLSLKVKKGDRIAAMMPNMPETVAAMLAA
ASIGAVWSSCSPDFGEQGVLDRFGQIEPVIFIGPDGYWYNGKAIEVADKVAAVAAKLGSVRKVLLVDYLGTSADVAATID
KAVAMEEALSPFAAKPVSFEPLPFSHPLYILFSSGTTGIPKCIVHSAGGTLIQHVKEHRLHAGLVDGDRFFYFTTCGWMM
WNWLVSGLASGATLLLYDGSPFYPDGNVLFDFVDAEKMTFFGTSAKFIDSVRKAGLKPIRSHDLSTVRAISSTGSPLSPE
DFRFVYEGIKKDVHLASVSGGTDIVSCFVLGVPNQPVWTGEIQGPGLGLAVDVWDDDGKPIRQEKGELVCTKAFPSMPIG
FWNDPEGKKYQAAYFERFDNVWCHGDFAEWTAHGGMIIHGRSDATLNPGGVRIGTAEIYNQVEQMPEILEALCIGQDFDN
DVRVVLFVRLAAGVELDEDLEKRIRAKIRSGASPRHVPARIVAVTDIPRTKSGKITELAVRDVVHGRAIKNKEALANPEA
LELFRNLPQLAE
>Mature_651_residues
AAEVPLWTPTQDRIDASPLTAFLKAAEAKAGATFASYADLHRWSVEDREGFWSLVWDFCGIAGEKGERLLADGDKMPGAS
FFPDATLNFAENLLKKTGAGEAIVFRGEDKVERRLSWNQLHGLVSRLQQLFLSLKVKKGDRIAAMMPNMPETVAAMLAAA
SIGAVWSSCSPDFGEQGVLDRFGQIEPVIFIGPDGYWYNGKAIEVADKVAAVAAKLGSVRKVLLVDYLGTSADVAATIDK
AVAMEEALSPFAAKPVSFEPLPFSHPLYILFSSGTTGIPKCIVHSAGGTLIQHVKEHRLHAGLVDGDRFFYFTTCGWMMW
NWLVSGLASGATLLLYDGSPFYPDGNVLFDFVDAEKMTFFGTSAKFIDSVRKAGLKPIRSHDLSTVRAISSTGSPLSPED
FRFVYEGIKKDVHLASVSGGTDIVSCFVLGVPNQPVWTGEIQGPGLGLAVDVWDDDGKPIRQEKGELVCTKAFPSMPIGF
WNDPEGKKYQAAYFERFDNVWCHGDFAEWTAHGGMIIHGRSDATLNPGGVRIGTAEIYNQVEQMPEILEALCIGQDFDND
VRVVLFVRLAAGVELDEDLEKRIRAKIRSGASPRHVPARIVAVTDIPRTKSGKITELAVRDVVHGRAIKNKEALANPEAL
ELFRNLPQLAE

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=656, Percent_Identity=42.9878048780488, Blast_Score=551, Evalue=1e-157,
Organism=Homo sapiens, GI8923896, Length=653, Percent_Identity=25.7274119448698, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI253970489, Length=666, Percent_Identity=25.3753753753754, Blast_Score=173, Evalue=6e-43,
Organism=Homo sapiens, GI28416953, Length=621, Percent_Identity=25.2818035426731, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI13375727, Length=617, Percent_Identity=23.8249594813614, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI38505220, Length=496, Percent_Identity=20.9677419354839, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI115511026, Length=528, Percent_Identity=21.4015151515152, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI42544132, Length=550, Percent_Identity=20, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1790505, Length=634, Percent_Identity=27.7602523659306, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1786529, Length=641, Percent_Identity=25.7410296411856, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17569507, Length=631, Percent_Identity=37.4009508716323, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71983001, Length=650, Percent_Identity=26, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71982997, Length=640, Percent_Identity=26.40625, Blast_Score=175, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6319264, Length=674, Percent_Identity=27.0029673590504, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6323182, Length=644, Percent_Identity=25.776397515528, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24667955, Length=660, Percent_Identity=26.3636363636364, Blast_Score=204, Evalue=2e-52,
Organism=Drosophila melanogaster, GI62472339, Length=565, Percent_Identity=26.9026548672566, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24667959, Length=528, Percent_Identity=26.8939393939394, Blast_Score=175, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21355225, Length=641, Percent_Identity=22.7769110764431, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 71155; Mature: 71024

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAEVPLWTPTQDRIDASPLTAFLKAAEAKAGATFASYADLHRWSVEDREGFWSLVWDFC
CCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
GIAGEKGERLLADGDKMPGASFFPDATLNFAENLLKKTGAGEAIVFRGEDKVERRLSWNQ
CCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
LHGLVSRLQQLFLSLKVKKGDRIAAMMPNMPETVAAMLAAASIGAVWSSCSPDFGEQGVL
HHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DRFGQIEPVIFIGPDGYWYNGKAIEVADKVAAVAAKLGSVRKVLLVDYLGTSADVAATID
HHCCCCCEEEEECCCCEEECCEEEHHHHHHHHHHHHHHHHHHEEEEHHCCCCCHHHHHHH
KAVAMEEALSPFAAKPVSFEPLPFSHPLYILFSSGTTGIPKCIVHSAGGTLIQHVKEHRL
HHHHHHHHHCCHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEECCCCHHHHHHHHHHH
HAGLVDGDRFFYFTTCGWMMWNWLVSGLASGATLLLYDGSPFYPDGNVLFDFVDAEKMTF
HCCEECCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCEEEE
FGTSAKFIDSVRKAGLKPIRSHDLSTVRAISSTGSPLSPEDFRFVYEGIKKDVHLASVSG
ECCCHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCC
GTDIVSCFVLGVPNQPVWTGEIQGPGLGLAVDVWDDDGKPIRQEKGELVCTKAFPSMPIG
CHHEEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCHHCCCCEEEEECCCCCCCC
FWNDPEGKKYQAAYFERFDNVWCHGDFAEWTAHGGMIIHGRSDATLNPGGVRIGTAEIYN
CCCCCCCCCCHHHHHHHHCCCEECCCHHHHHCCCCEEEECCCCCEECCCCEEECHHHHHH
QVEQMPEILEALCIGQDFDNDVRVVLFVRLAAGVELDEDLEKRIRAKIRSGASPRHVPAR
HHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
IVAVTDIPRTKSGKITELAVRDVVHGRAIKNKEALANPEALELFRNLPQLAE
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCHHHCC
>Mature Secondary Structure 
AAEVPLWTPTQDRIDASPLTAFLKAAEAKAGATFASYADLHRWSVEDREGFWSLVWDFC
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
GIAGEKGERLLADGDKMPGASFFPDATLNFAENLLKKTGAGEAIVFRGEDKVERRLSWNQ
CCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
LHGLVSRLQQLFLSLKVKKGDRIAAMMPNMPETVAAMLAAASIGAVWSSCSPDFGEQGVL
HHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DRFGQIEPVIFIGPDGYWYNGKAIEVADKVAAVAAKLGSVRKVLLVDYLGTSADVAATID
HHCCCCCEEEEECCCCEEECCEEEHHHHHHHHHHHHHHHHHHEEEEHHCCCCCHHHHHHH
KAVAMEEALSPFAAKPVSFEPLPFSHPLYILFSSGTTGIPKCIVHSAGGTLIQHVKEHRL
HHHHHHHHHCCHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEECCCCHHHHHHHHHHH
HAGLVDGDRFFYFTTCGWMMWNWLVSGLASGATLLLYDGSPFYPDGNVLFDFVDAEKMTF
HCCEECCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCEEEE
FGTSAKFIDSVRKAGLKPIRSHDLSTVRAISSTGSPLSPEDFRFVYEGIKKDVHLASVSG
ECCCHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCC
GTDIVSCFVLGVPNQPVWTGEIQGPGLGLAVDVWDDDGKPIRQEKGELVCTKAFPSMPIG
CHHEEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCHHCCCCEEEEECCCCCCCC
FWNDPEGKKYQAAYFERFDNVWCHGDFAEWTAHGGMIIHGRSDATLNPGGVRIGTAEIYN
CCCCCCCCCCHHHHHHHHCCCEECCCHHHHHCCCCEEEECCCCCEECCCCEEECHHHHHH
QVEQMPEILEALCIGQDFDNDVRVVLFVRLAAGVELDEDLEKRIRAKIRSGASPRHVPAR
HHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
IVAVTDIPRTKSGKITELAVRDVVHGRAIKNKEALANPEALELFRNLPQLAE
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]