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The map label for this gene is melA [H]

Identifier: 13475389

GI number: 13475389

Start: 5264485

End: 5265951

Strand: Direct

Name: melA [H]

Synonym: mlr6443

Alternate gene names: 13475389

Gene position: 5264485-5265951 (Clockwise)

Preceding gene: 13475387

Following gene: 13475390

Centisome position: 74.82

GC content: 60.67

Gene sequence:

>1467_bases
ATGAGTTTCAAAATCGCTATCATCGGTGCCGGCAGCGTCGGATTCACCAAGAAACTGTTCACCGACATCCTGTGCGTGCC
CGAATTCAGGGATATCGAATTCGCACTGACCGACCTCAGCGAACACAATCTCGGGATGATCAAGGCGATTCTCGACAAGG
TCGTCGAGGCGAACGGGTTGCCAACCAGGGTGACGGCGACAACCGATCGCCGCAAGGCGCTCGAAGGCGCGCGCTATATC
ATCAGTTGTGTGCGGGTCGGCGGCCTCGAGGCCTATGCCGACGACATTCGCATTCCGCTGAAATATGGCATCGACCAGTG
CGTCGGCGACACCATCTGCGCAGGCGGCATCCTCTATGGCCAGCGCAACATTCCCGTGATCCTCGACTTCTGCAAGGACA
TACGCGAGGTCGCCGCGACGGGCGCCAAATTCCTCAACTATGCCAACCCGATGGCCATGAACACTTGGGCGGCGATCGAA
TACGGCAAGGTAGACACGGTCGGCCTCTGCCACGGCGTCCAGCATGGCGCCGAGCAGATCGCCGAGGTGCTGGGAGCGAA
GTCCAGGCACGAGCTCGACTATGTCTGTTCCGGCATCAATCACCAGACCTGGTTCATCGATTTGCGGCTGAATGGCCGCA
AGATCGCCAAGGACGAACTGGTCGAAGCGTTCGAGGCGCATCCGATCTATTCGCAGCAGGAAAAGCTGCGCATCGATGTC
TTGAAGCGCTTCGGTGTCTATTCGACCGAGAGCAACGGCCATCTCTCCGAATATCTGCCCTGGTACCGCAAGCGGCCCGA
CGAGATCACCCGCTGGATCGATATGTCGGACTGGATCCATGGCGAGACCGGCGGCTATCTCCGCTACTCCACCGAAACCC
GCAACTGGTTCGAGACGGAGTATCCGCAATTCCTCGAAGCGGCGTCGAAGCCGATCGATCCGGCCAAGCGCTCCAACGAA
CACGCCAGCCACATATTGGAGGCGCTGGAGACCAATCGGGTCTATCGTGGCCACTTCAACGTCAGGAATGATGGCCTGAT
CACCAATCTGCCGCAGGATGCCATCGTCGAGTCGCCCGGCTTCGTCGACCGTTTCGGCATCAACATGGCCGCCGGCATCA
CATTGCCGGAAGCCTGTGCGGCCACCTGCATGTCTTCGATCAACGTCCAGCGCATGTCGGTGCATGCCGCGATATCAGGC
GACATCGATCTCCTGAAGCTCGCCGTGCTGCACGACCCGCTGGTCGGCGCGGTGTCGACGCCGGAGGAAGTCTGGCAGAT
GGTGGACGAAATGGTCGTCGCCCAGGCTCAATGGCTGCCGCAATATGCGCATGCGGTTCCGGCCGCCAAGGAGCGGCTGT
CGAAATCCGCGGTCAAGACCCGCGAATGGGCGGGTGCTGCGCGGCGAAACGTGCGCTCGATCGAGGAATTGCGCGCCGAA
AAGGCCGCGCTCAAACAGGCCGGCTGA

Upstream 100 bases:

>100_bases
GGCCGGAAACCGGCTTGTCCCGCACACATGCCATGGCATGCGGCTGCGACCTACCGCTGTCATTCCCGGCCTGCCATTGG
AATAGGATGAGGTACGGCTG

Downstream 100 bases:

>100_bases
AACCCCGGGCGCAGCGACGGACCTTGGGAGGCAGGTCCATCGCATTGAGCCGTCCAGGCAGAGACGAGCAGCAGAAAACG
TCTGAACATGAGGGAGGAGA

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSFKIAIIGAGSVGFTKKLFTDILCVPEFRDIEFALTDLSEHNLGMIKAILDKVVEANGLPTRVTATTDRRKALEGARYI
ISCVRVGGLEAYADDIRIPLKYGIDQCVGDTICAGGILYGQRNIPVILDFCKDIREVAATGAKFLNYANPMAMNTWAAIE
YGKVDTVGLCHGVQHGAEQIAEVLGAKSRHELDYVCSGINHQTWFIDLRLNGRKIAKDELVEAFEAHPIYSQQEKLRIDV
LKRFGVYSTESNGHLSEYLPWYRKRPDEITRWIDMSDWIHGETGGYLRYSTETRNWFETEYPQFLEAASKPIDPAKRSNE
HASHILEALETNRVYRGHFNVRNDGLITNLPQDAIVESPGFVDRFGINMAAGITLPEACAATCMSSINVQRMSVHAAISG
DIDLLKLAVLHDPLVGAVSTPEEVWQMVDEMVVAQAQWLPQYAHAVPAAKERLSKSAVKTREWAGAARRNVRSIEELRAE
KAALKQAG

Sequences:

>Translated_488_residues
MSFKIAIIGAGSVGFTKKLFTDILCVPEFRDIEFALTDLSEHNLGMIKAILDKVVEANGLPTRVTATTDRRKALEGARYI
ISCVRVGGLEAYADDIRIPLKYGIDQCVGDTICAGGILYGQRNIPVILDFCKDIREVAATGAKFLNYANPMAMNTWAAIE
YGKVDTVGLCHGVQHGAEQIAEVLGAKSRHELDYVCSGINHQTWFIDLRLNGRKIAKDELVEAFEAHPIYSQQEKLRIDV
LKRFGVYSTESNGHLSEYLPWYRKRPDEITRWIDMSDWIHGETGGYLRYSTETRNWFETEYPQFLEAASKPIDPAKRSNE
HASHILEALETNRVYRGHFNVRNDGLITNLPQDAIVESPGFVDRFGINMAAGITLPEACAATCMSSINVQRMSVHAAISG
DIDLLKLAVLHDPLVGAVSTPEEVWQMVDEMVVAQAQWLPQYAHAVPAAKERLSKSAVKTREWAGAARRNVRSIEELRAE
KAALKQAG
>Mature_487_residues
SFKIAIIGAGSVGFTKKLFTDILCVPEFRDIEFALTDLSEHNLGMIKAILDKVVEANGLPTRVTATTDRRKALEGARYII
SCVRVGGLEAYADDIRIPLKYGIDQCVGDTICAGGILYGQRNIPVILDFCKDIREVAATGAKFLNYANPMAMNTWAAIEY
GKVDTVGLCHGVQHGAEQIAEVLGAKSRHELDYVCSGINHQTWFIDLRLNGRKIAKDELVEAFEAHPIYSQQEKLRIDVL
KRFGVYSTESNGHLSEYLPWYRKRPDEITRWIDMSDWIHGETGGYLRYSTETRNWFETEYPQFLEAASKPIDPAKRSNEH
ASHILEALETNRVYRGHFNVRNDGLITNLPQDAIVESPGFVDRFGINMAAGITLPEACAATCMSSINVQRMSVHAAISGD
IDLLKLAVLHDPLVGAVSTPEEVWQMVDEMVVAQAQWLPQYAHAVPAAKERLSKSAVKTREWAGAARRNVRSIEELRAEK
AALKQAG

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=461, Percent_Identity=33.6225596529284, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788029, Length=459, Percent_Identity=26.1437908496732, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 54322; Mature: 54190

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKIAIIGAGSVGFTKKLFTDILCVPEFRDIEFALTDLSEHNLGMIKAILDKVVEANGL
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
PTRVTATTDRRKALEGARYIISCVRVGGLEAYADDIRIPLKYGIDQCVGDTICAGGILYG
CCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHCHHHHHCHHHHCCCEEEC
QRNIPVILDFCKDIREVAATGAKFLNYANPMAMNTWAAIEYGKVDTVGLCHGVQHGAEQI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHH
AEVLGAKSRHELDYVCSGINHQTWFIDLRLNGRKIAKDELVEAFEAHPIYSQQEKLRIDV
HHHHCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
LKRFGVYSTESNGHLSEYLPWYRKRPDEITRWIDMSDWIHGETGGYLRYSTETRNWFETE
HHHCCCCCCCCCCCHHHHCCHHHCCHHHHHHHHCHHHCCCCCCCCEEEEECCCCCCHHCC
YPQFLEAASKPIDPAKRSNEHASHILEALETNRVYRGHFNVRNDGLITNLPQDAIVESPG
CHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHCCCC
FVDRFGINMAAGITLPEACAATCMSSINVQRMSVHAAISGDIDLLKLAVLHDPLVGAVST
CHHHHCCCEEECCCCHHHHHHHHHHHCCHHHEEEEEEECCCHHHHHHHHHHCCCCCCCCC
PEEVWQMVDEMVVAQAQWLPQYAHAVPAAKERLSKSAVKTREWAGAARRNVRSIEELRAE
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAALKQAG
HHHHHCCC
>Mature Secondary Structure 
SFKIAIIGAGSVGFTKKLFTDILCVPEFRDIEFALTDLSEHNLGMIKAILDKVVEANGL
CEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
PTRVTATTDRRKALEGARYIISCVRVGGLEAYADDIRIPLKYGIDQCVGDTICAGGILYG
CCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHCHHHHHCHHHHCCCEEEC
QRNIPVILDFCKDIREVAATGAKFLNYANPMAMNTWAAIEYGKVDTVGLCHGVQHGAEQI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHH
AEVLGAKSRHELDYVCSGINHQTWFIDLRLNGRKIAKDELVEAFEAHPIYSQQEKLRIDV
HHHHCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
LKRFGVYSTESNGHLSEYLPWYRKRPDEITRWIDMSDWIHGETGGYLRYSTETRNWFETE
HHHCCCCCCCCCCCHHHHCCHHHCCHHHHHHHHCHHHCCCCCCCCEEEEECCCCCCHHCC
YPQFLEAASKPIDPAKRSNEHASHILEALETNRVYRGHFNVRNDGLITNLPQDAIVESPG
CHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHCCCC
FVDRFGINMAAGITLPEACAATCMSSINVQRMSVHAAISGDIDLLKLAVLHDPLVGAVST
CHHHHCCCEEECCCCHHHHHHHHHHHCCHHHEEEEEEECCCHHHHHHHHHHCCCCCCCCC
PEEVWQMVDEMVVAQAQWLPQYAHAVPAAKERLSKSAVKTREWAGAARRNVRSIEELRAE
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAALKQAG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9791127; 11481431 [H]