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The map label for this gene is traG [H]

Identifier: 13475349

GI number: 13475349

Start: 5217849

End: 5220056

Strand: Direct

Name: traG [H]

Synonym: mlr6395

Alternate gene names: 13475349

Gene position: 5217849-5220056 (Clockwise)

Preceding gene: 13475348

Following gene: 13475351

Centisome position: 74.16

GC content: 61.05

Gene sequence:

>2208_bases
ATGAGTCCCCTCCGACATAGCAGTCCACGGGAATGCGCGAATATTGATACACCTCGAAAGAGAGCAACGGCGCGCCAAAC
AGCCCCTCATCGCACCAAGTCGTACATAGGGCGCATTCCTGTTCCGGCCGTCCCGGGCGATGCTCGATCCATCTCTCCAG
AACAGCGGAGCAAGCCAGTGACGCCCACGAAGCTTCTGATTGGGCAGATCGCCATTGTGTTCGCCATCGTTCTCCTCGGC
GTGTGGGCTGCCACGCAATGGTGCGCTCATATGCTAGCCTTTCAGGAACAACTCGGCGCGCCATGGTTTGTCGCGGCTGG
CTGGCCGATCTACGAGCCGTGGAAACTGTTGGAATGGTGGTTCCAATTCGATGCCTATGCGCCCGAGGTATTCGACAAGG
CGGGCATGCTTGCGGGCACCAGTGGATTCATGGGCTGTGCCGCTGCGATCGCTGGTTCCCTCTGGCGCGCGCGCCAGCGC
GGCTTGGTCACCACCTATGGCTCCTCTCGATGGGCGGCGACGCGGGAGATCGAGAAAGCAGGGCTGTTTCAGCCGGCAGG
CGTCTTCCTCGGCAAACTGAAGGACCGGTATCTGCGTCATGACGGGCCTGAGCACGTCATGGCCTTTGCGCCGACACGTT
CCGGCAAGGGCGTCGGACTGGTTGTCCCAACCCTTCTCTCCTGGTCCGGCTCGGCCGTCATTCACGACATCAAAGGCGAG
AACTGGCAGCTGAGCTCCGGCTGGCGGTCGAGGTTCTCGCATTGCCTTTTATTCAATCCGACTGATCCGCGATCGGCCCG
CTACAACCCGCTGCTGGAGGTACGCAAAGGTTCGGACGAGATTCGCGATGTCCAAAACATCGCCGACATTCTAGTCGATC
CGGAAGGCGCGCTGGAGCGGCGGAATCATTGGGAGAAGACCAGTCATTCACTCCTGGTCGGCGCTATCTTACACGTCCTC
TACGCCGAAGAGGAAAAGACTCTTGCCCGCGTCGCCACCTTTCTGTCGGACCCGCAACGCTCGTTTGCCGCGACTTTGCG
GCGAATGATGACGACCAACCATCTCGGCGCGGCCAATAAGCCTCAGGTCCATCCCGTCGTGGCCTCGGCGGCACGAGAGG
TGTTGAACAAATCGGAGAACGAACGTTCAGGCGTCCTCTCGACCGCCATGTCTTTTCTCGGCCTCTACCGTGATCCGACC
GTGGCGGCGGTAACATCGGACTGCGACTGGCGCATTGCCGATCTGATGGATGCGGAGTGGCCGATGTCGCTCTATCTGGT
CGTGCCGCCATCGGACATCTCGCGCACCAAGCCCTTGGTGCGACTGATCCTGAACCAGATCGGCAGGCGGCTGACGGAGC
GCCTCGAGGGTGACCCAAAGAAGAGCCGCAAGCATCAACTGCTCATGATGCTGGATGAGTTTCCGGCGCTCGGTCGCCTC
GACTTCTTTGAGACCGCGCTCGCGTTTATGGCCGGTTACGGAATTCGTGCCTACTTGATCGCGCAATCGTTGAACCAGAT
CTCCAAAGCGTATGGCGACAACAACGCCATTCTGGACAACTGCCATTTGCGAATTGCCTTTTCCTCCAATGATGAGCGCA
CGGCCAAGCGAATCTCGGACGCGCTTGGCACGGCCACGGAACTCAGATCCATGCGCAACTATGCGGGCCACCGGCTGGCA
CCTTGGCTCTCGCATGTCATGGTGAGCCGCCAGGAAACCGCCCGCCCGCTGCTGACACCGGGCGAGGTGATGCAATTGCC
GCCCGCGGACGAACTGGTGCTGGTTTCGGGATTGCCCCCCATCCGGGCCAAAAAGCTGCGATACTATGAGGATCACAATT
TCACCGAGCGGGTGCTACCTTCGCCGGTGTTGCGCGACGGACCCTACGCGGACTGCCCGATGTCGCGGCCGGACGATTGG
ACTGGGCAAGTACGCGGCGTCGACCGTCGTCTTGCGTCCGACGATGAAAGCGCTGGCGCCGCGCTTGCGGACGAGGGAGG
TGTTCAGCAGCAGCGCCATCCTGGCCTACCCGAAGAACATACCGCCGTCACCCAGGAACCGGACCAGGACGATCTGTCCA
GACCCGCAGACGATGATAGCGAGGCGATGGCGGACAAGCGCGTCATGGACCGGATCTCCACCGTCGCAGGTGCCTATGGC
ATCAACGAGGGAAGGGGCGATGATAAGGATATCGTCCCCGGCTTCTGA

Upstream 100 bases:

>100_bases
TGTACCTCAGACCGGACTCGATGTTACGAATTTCCTCAGCGGCTTGCCCGCATGCCACCGCCAGCTCCCCGACCACGTGC
TCACGATAGGCACGAACGAT

Downstream 100 bases:

>100_bases
ACGCTTTCCGCAGCTCGGGCGTATCTCAGCGTAGATAACGGCCGTAAGCAATTTTGGGCATCTCGATAATGCTTGTTCCG
CCGCCGGCCCGCCAGCGCCG

Product: conjugal transfer coupling protein TraG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 735; Mature: 734

Protein sequence:

>735_residues
MSPLRHSSPRECANIDTPRKRATARQTAPHRTKSYIGRIPVPAVPGDARSISPEQRSKPVTPTKLLIGQIAIVFAIVLLG
VWAATQWCAHMLAFQEQLGAPWFVAAGWPIYEPWKLLEWWFQFDAYAPEVFDKAGMLAGTSGFMGCAAAIAGSLWRARQR
GLVTTYGSSRWAATREIEKAGLFQPAGVFLGKLKDRYLRHDGPEHVMAFAPTRSGKGVGLVVPTLLSWSGSAVIHDIKGE
NWQLSSGWRSRFSHCLLFNPTDPRSARYNPLLEVRKGSDEIRDVQNIADILVDPEGALERRNHWEKTSHSLLVGAILHVL
YAEEEKTLARVATFLSDPQRSFAATLRRMMTTNHLGAANKPQVHPVVASAAREVLNKSENERSGVLSTAMSFLGLYRDPT
VAAVTSDCDWRIADLMDAEWPMSLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPKKSRKHQLLMMLDEFPALGRL
DFFETALAFMAGYGIRAYLIAQSLNQISKAYGDNNAILDNCHLRIAFSSNDERTAKRISDALGTATELRSMRNYAGHRLA
PWLSHVMVSRQETARPLLTPGEVMQLPPADELVLVSGLPPIRAKKLRYYEDHNFTERVLPSPVLRDGPYADCPMSRPDDW
TGQVRGVDRRLASDDESAGAALADEGGVQQQRHPGLPEEHTAVTQEPDQDDLSRPADDDSEAMADKRVMDRISTVAGAYG
INEGRGDDKDIVPGF

Sequences:

>Translated_735_residues
MSPLRHSSPRECANIDTPRKRATARQTAPHRTKSYIGRIPVPAVPGDARSISPEQRSKPVTPTKLLIGQIAIVFAIVLLG
VWAATQWCAHMLAFQEQLGAPWFVAAGWPIYEPWKLLEWWFQFDAYAPEVFDKAGMLAGTSGFMGCAAAIAGSLWRARQR
GLVTTYGSSRWAATREIEKAGLFQPAGVFLGKLKDRYLRHDGPEHVMAFAPTRSGKGVGLVVPTLLSWSGSAVIHDIKGE
NWQLSSGWRSRFSHCLLFNPTDPRSARYNPLLEVRKGSDEIRDVQNIADILVDPEGALERRNHWEKTSHSLLVGAILHVL
YAEEEKTLARVATFLSDPQRSFAATLRRMMTTNHLGAANKPQVHPVVASAAREVLNKSENERSGVLSTAMSFLGLYRDPT
VAAVTSDCDWRIADLMDAEWPMSLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPKKSRKHQLLMMLDEFPALGRL
DFFETALAFMAGYGIRAYLIAQSLNQISKAYGDNNAILDNCHLRIAFSSNDERTAKRISDALGTATELRSMRNYAGHRLA
PWLSHVMVSRQETARPLLTPGEVMQLPPADELVLVSGLPPIRAKKLRYYEDHNFTERVLPSPVLRDGPYADCPMSRPDDW
TGQVRGVDRRLASDDESAGAALADEGGVQQQRHPGLPEEHTAVTQEPDQDDLSRPADDDSEAMADKRVMDRISTVAGAYG
INEGRGDDKDIVPGF
>Mature_734_residues
SPLRHSSPRECANIDTPRKRATARQTAPHRTKSYIGRIPVPAVPGDARSISPEQRSKPVTPTKLLIGQIAIVFAIVLLGV
WAATQWCAHMLAFQEQLGAPWFVAAGWPIYEPWKLLEWWFQFDAYAPEVFDKAGMLAGTSGFMGCAAAIAGSLWRARQRG
LVTTYGSSRWAATREIEKAGLFQPAGVFLGKLKDRYLRHDGPEHVMAFAPTRSGKGVGLVVPTLLSWSGSAVIHDIKGEN
WQLSSGWRSRFSHCLLFNPTDPRSARYNPLLEVRKGSDEIRDVQNIADILVDPEGALERRNHWEKTSHSLLVGAILHVLY
AEEEKTLARVATFLSDPQRSFAATLRRMMTTNHLGAANKPQVHPVVASAAREVLNKSENERSGVLSTAMSFLGLYRDPTV
AAVTSDCDWRIADLMDAEWPMSLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPKKSRKHQLLMMLDEFPALGRLD
FFETALAFMAGYGIRAYLIAQSLNQISKAYGDNNAILDNCHLRIAFSSNDERTAKRISDALGTATELRSMRNYAGHRLAP
WLSHVMVSRQETARPLLTPGEVMQLPPADELVLVSGLPPIRAKKLRYYEDHNFTERVLPSPVLRDGPYADCPMSRPDDWT
GQVRGVDRRLASDDESAGAALADEGGVQQQRHPGLPEEHTAVTQEPDQDDLSRPADDDSEAMADKRVMDRISTVAGAYGI
NEGRGDDKDIVPGF

Specific function: Required for conjugative transfer of plasmid RP4. Binds tightly and specifically to the relaxase traI. Can also bind to DNA without sequence specificity. May form a pore-like structure that could serve as a channel for DNA transfer [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virD4/traG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003688 [H]

Pfam domain/function: PF02534 TraG [H]

EC number: NA

Molecular weight: Translated: 81572; Mature: 81441

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLRHSSPRECANIDTPRKRATARQTAPHRTKSYIGRIPVPAVPGDARSISPEQRSKPV
CCCCCCCCCCHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCC
TPTKLLIGQIAIVFAIVLLGVWAATQWCAHMLAFQEQLGAPWFVAAGWPIYEPWKLLEWW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
FQFDAYAPEVFDKAGMLAGTSGFMGCAAAIAGSLWRARQRGLVTTYGSSRWAATREIEKA
HHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
GLFQPAGVFLGKLKDRYLRHDGPEHVMAFAPTRSGKGVGLVVPTLLSWSGSAVIHDIKGE
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHCCCCCEEEEECCCC
NWQLSSGWRSRFSHCLLFNPTDPRSARYNPLLEVRKGSDEIRDVQNIADILVDPEGALER
CEEECCCHHHHCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
RNHWEKTSHSLLVGAILHVLYAEEEKTLARVATFLSDPQRSFAATLRRMMTTNHLGAANK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
PQVHPVVASAAREVLNKSENERSGVLSTAMSFLGLYRDPTVAAVTSDCDWRIADLMDAEW
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHCCCCC
PMSLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPKKSRKHQLLMMLDEFPALGRL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCH
DFFETALAFMAGYGIRAYLIAQSLNQISKAYGDNNAILDNCHLRIAFSSNDERTAKRISD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCHHHHHHHHH
ALGTATELRSMRNYAGHRLAPWLSHVMVSRQETARPLLTPGEVMQLPPADELVLVSGLPP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEECCCC
IRAKKLRYYEDHNFTERVLPSPVLRDGPYADCPMSRPDDWTGQVRGVDRRLASDDESAGA
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
ALADEGGVQQQRHPGLPEEHTAVTQEPDQDDLSRPADDDSEAMADKRVMDRISTVAGAYG
CCCCCCCCCHHCCCCCCHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
INEGRGDDKDIVPGF
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPLRHSSPRECANIDTPRKRATARQTAPHRTKSYIGRIPVPAVPGDARSISPEQRSKPV
CCCCCCCCCHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCC
TPTKLLIGQIAIVFAIVLLGVWAATQWCAHMLAFQEQLGAPWFVAAGWPIYEPWKLLEWW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
FQFDAYAPEVFDKAGMLAGTSGFMGCAAAIAGSLWRARQRGLVTTYGSSRWAATREIEKA
HHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
GLFQPAGVFLGKLKDRYLRHDGPEHVMAFAPTRSGKGVGLVVPTLLSWSGSAVIHDIKGE
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHCCCCCEEEEECCCC
NWQLSSGWRSRFSHCLLFNPTDPRSARYNPLLEVRKGSDEIRDVQNIADILVDPEGALER
CEEECCCHHHHCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
RNHWEKTSHSLLVGAILHVLYAEEEKTLARVATFLSDPQRSFAATLRRMMTTNHLGAANK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
PQVHPVVASAAREVLNKSENERSGVLSTAMSFLGLYRDPTVAAVTSDCDWRIADLMDAEW
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHCCCCC
PMSLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPKKSRKHQLLMMLDEFPALGRL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCH
DFFETALAFMAGYGIRAYLIAQSLNQISKAYGDNNAILDNCHLRIAFSSNDERTAKRISD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCHHHHHHHHH
ALGTATELRSMRNYAGHRLAPWLSHVMVSRQETARPLLTPGEVMQLPPADELVLVSGLPP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEECCCC
IRAKKLRYYEDHNFTERVLPSPVLRDGPYADCPMSRPDDWTGQVRGVDRRLASDDESAGA
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
ALADEGGVQQQRHPGLPEEHTAVTQEPDQDDLSRPADDDSEAMADKRVMDRISTVAGAYG
CCCCCCCCCHHCCCCCCHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
INEGRGDDKDIVPGF
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1665997; 8021214; 11976307 [H]