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The map label for this gene is potH [H]

Identifier: 13475227

GI number: 13475227

Start: 5073350

End: 5075152

Strand: Reverse

Name: potH [H]

Synonym: mll6253

Alternate gene names: 13475227

Gene position: 5075152-5073350 (Counterclockwise)

Preceding gene: 13475228

Following gene: 13475226

Centisome position: 72.13

GC content: 51.97

Gene sequence:

>1803_bases
ATGACTATGGCCAAATTTAATCTGGCACTTCGCGGCGCGAAACTAGCGCCGAACACAAGCCGAGAGGCCTTCGTGCTCCT
CGCTCCGGCAACTGTCATTCTAGGCGTGTGTTTCTTCATCCCACTGGCCGGCCTTGTCGCGCTCAGCTTCCTTAGTCCTG
AACCAACGCTGATGCACTACAAGGAATTTCTTGGAAGCTGGACAAGTTGGGTAATACTCGGACGGACATTTTCTCTTGCA
CTGAGCGTTACCAGTATCTGCTTATTGCTTGGCTATCCGCTCGCCTACTGGATCGTTTCGGCCCGCGCCTGGGTCTCAGG
CCTGCTTGTATTTGCAGTAGTCCTGCCTTTTTTTACAAGCTATCTGGTCAGAAGTTACGCTTGGATGGTTCTGCTCGGTC
GATTTGGCATCATCAATCAGTTTTTGATGAAAGTTGGCTTTACCGAGCGCCCGTTGGACCTGATCTACAATCAATTCAGC
GTAGACGTTGCAATGGTCTATATGCTGTTGCCCTTTACGGTGATGGTGCTTTCAAGTGTGATGAGAGGGATCTCGCCGAG
TCTCACAAGAGCGGCATCATCGCTTGGTGCAGCGCCAGGCCAGAATTTCTGGCGAATATTTTTCCCCCTTAGCATGCCCG
GTGTTGTGGCATCGTCTCTGATCGTTTTCGTATTTGCGCTTGGTTTTTATATAACACCGGCATTGCTAGGTGGTCCGCGC
AACGTCACGTTGCCCATGCTGATCGATACCTATGTCAACGGCACCTTGAACTGGCCGCTTGCGGCCGCTGTCGCAACGGT
CCTGCTTCTCATTACGCTCTTCCTTTATACGGTCTCAAACCGCTTCGTGGGTACAGAAAGACTTCTTGGTTCCGGGCATC
TTGTGGCCTCTTCAAAGCCGTCGACCATCGGCCGATCGACAAGTGGATATGTAAAGTTTGTCGATACGCTTAGTTCGGCT
GTGAGAGTGATTTCGTACTTCATCCCGTCATCCTTTTCGAGTGTTTGGGAGAGGGCGGCGATAGGAAGAATCATATTTTA
CTGCGTCGGGCTAGCAGTCGTTGCATTTCTTGTATGCCCGATTTTTATTATTATACCGATATCGTTCAGTTCCTCGCGCT
TCCTCGATTTTCCCCCTCCAGGCCTCTCGCTACAGTGGTATGAAGCATACTTCACCAACGGCTTATGGTTGTCGTCTACC
CGGCAGAGCCTCATTGTTGGTTTCGCGACGGCATTTGTTAGTCTCACAGTCGGAACAGCTGCGGCTTTGGCTCTGGTTCG
CGGGAGCGCCCGCATGCGCCGGATTGGGTATCAACTGCTACTCGCGCCTATGATCGTGCCTTCTATCGTCAGCGCTGTGG
CGGTCTACTTCTTGTTTTCAAAATTGGGGTTGGTCGACACCACGATGGGGTTGGTGCTTGCACATAGCATCGGTGCAACC
CCCTTGGTGGTCCTCATCGTTGCGGCGAGCCTCCAATCTCAGAACATTCGCCTCGAGCACGCAGCTGCGAGCCTGGGCGC
TTCTAGGATCGTCACTATTCGGAAGGTGGTCCTGCCGCTCATTCGACCCACGCTCTTCGTGGCGGGGTTCTTCGCGTTCC
TTCATTCGTTTGATGAAGTGGTCTTGTCGCTTTTTGTCAGCGGGCCGAATACGATGACTTTGCCGATCAAGATGTGGTCT
GGAGTTCGTGAAGACATAACGCCAACCATCGCAGCGGTATCTAGCCTTCTGATTGGCTTCACAGTTATTCTATATGGAAC
GATTGAAGTCGTTCGCCTAAGAAACAAGCGTAAATCAAAGTGA

Upstream 100 bases:

>100_bases
CTCGAGATTCCCGCCTTCTCGTCGATGAAAGCGAACGTCAAGCTGGAAATGCGCCAGAACCCGAACTGCTCATCGGTCAG
GGGATTGAGCGGGTGGTTTC

Downstream 100 bases:

>100_bases
AATTGGTTGTGTATCGAACGTTATCTGCGATCAGAGCGTGTTGCACTTCGTTCGTACCAACTGGACAGCATCATAACCTT
TAGGAGAGCGGTAGCATGGA

Product: ABC transporter permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MTMAKFNLALRGAKLAPNTSREAFVLLAPATVILGVCFFIPLAGLVALSFLSPEPTLMHYKEFLGSWTSWVILGRTFSLA
LSVTSICLLLGYPLAYWIVSARAWVSGLLVFAVVLPFFTSYLVRSYAWMVLLGRFGIINQFLMKVGFTERPLDLIYNQFS
VDVAMVYMLLPFTVMVLSSVMRGISPSLTRAASSLGAAPGQNFWRIFFPLSMPGVVASSLIVFVFALGFYITPALLGGPR
NVTLPMLIDTYVNGTLNWPLAAAVATVLLLITLFLYTVSNRFVGTERLLGSGHLVASSKPSTIGRSTSGYVKFVDTLSSA
VRVISYFIPSSFSSVWERAAIGRIIFYCVGLAVVAFLVCPIFIIIPISFSSSRFLDFPPPGLSLQWYEAYFTNGLWLSST
RQSLIVGFATAFVSLTVGTAAALALVRGSARMRRIGYQLLLAPMIVPSIVSAVAVYFLFSKLGLVDTTMGLVLAHSIGAT
PLVVLIVAASLQSQNIRLEHAAASLGASRIVTIRKVVLPLIRPTLFVAGFFAFLHSFDEVVLSLFVSGPNTMTLPIKMWS
GVREDITPTIAAVSSLLIGFTVILYGTIEVVRLRNKRKSK

Sequences:

>Translated_600_residues
MTMAKFNLALRGAKLAPNTSREAFVLLAPATVILGVCFFIPLAGLVALSFLSPEPTLMHYKEFLGSWTSWVILGRTFSLA
LSVTSICLLLGYPLAYWIVSARAWVSGLLVFAVVLPFFTSYLVRSYAWMVLLGRFGIINQFLMKVGFTERPLDLIYNQFS
VDVAMVYMLLPFTVMVLSSVMRGISPSLTRAASSLGAAPGQNFWRIFFPLSMPGVVASSLIVFVFALGFYITPALLGGPR
NVTLPMLIDTYVNGTLNWPLAAAVATVLLLITLFLYTVSNRFVGTERLLGSGHLVASSKPSTIGRSTSGYVKFVDTLSSA
VRVISYFIPSSFSSVWERAAIGRIIFYCVGLAVVAFLVCPIFIIIPISFSSSRFLDFPPPGLSLQWYEAYFTNGLWLSST
RQSLIVGFATAFVSLTVGTAAALALVRGSARMRRIGYQLLLAPMIVPSIVSAVAVYFLFSKLGLVDTTMGLVLAHSIGAT
PLVVLIVAASLQSQNIRLEHAAASLGASRIVTIRKVVLPLIRPTLFVAGFFAFLHSFDEVVLSLFVSGPNTMTLPIKMWS
GVREDITPTIAAVSSLLIGFTVILYGTIEVVRLRNKRKSK
>Mature_599_residues
TMAKFNLALRGAKLAPNTSREAFVLLAPATVILGVCFFIPLAGLVALSFLSPEPTLMHYKEFLGSWTSWVILGRTFSLAL
SVTSICLLLGYPLAYWIVSARAWVSGLLVFAVVLPFFTSYLVRSYAWMVLLGRFGIINQFLMKVGFTERPLDLIYNQFSV
DVAMVYMLLPFTVMVLSSVMRGISPSLTRAASSLGAAPGQNFWRIFFPLSMPGVVASSLIVFVFALGFYITPALLGGPRN
VTLPMLIDTYVNGTLNWPLAAAVATVLLLITLFLYTVSNRFVGTERLLGSGHLVASSKPSTIGRSTSGYVKFVDTLSSAV
RVISYFIPSSFSSVWERAAIGRIIFYCVGLAVVAFLVCPIFIIIPISFSSSRFLDFPPPGLSLQWYEAYFTNGLWLSSTR
QSLIVGFATAFVSLTVGTAAALALVRGSARMRRIGYQLLLAPMIVPSIVSAVAVYFLFSKLGLVDTTMGLVLAHSIGATP
LVVLIVAASLQSQNIRLEHAAASLGASRIVTIRKVVLPLIRPTLFVAGFFAFLHSFDEVVLSLFVSGPNTMTLPIKMWSG
VREDITPTIAAVSSLLIGFTVILYGTIEVVRLRNKRKSK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=216, Percent_Identity=32.8703703703704, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=31.6602316602317, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1787368, Length=233, Percent_Identity=30.4721030042918, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787713, Length=240, Percent_Identity=29.5833333333333, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1787081, Length=272, Percent_Identity=27.9411764705882, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787714, Length=204, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 65246; Mature: 65114

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMAKFNLALRGAKLAPNTSREAFVLLAPATVILGVCFFIPLAGLVALSFLSPEPTLMHY
CCCCHHHHHEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KEFLGSWTSWVILGRTFSLALSVTSICLLLGYPLAYWIVSARAWVSGLLVFAVVLPFFTS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLVRSYAWMVLLGRFGIINQFLMKVGFTERPLDLIYNQFSVDVAMVYMLLPFTVMVLSSV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRGISPSLTRAASSLGAAPGQNFWRIFFPLSMPGVVASSLIVFVFALGFYITPALLGGPR
HHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NVTLPMLIDTYVNGTLNWPLAAAVATVLLLITLFLYTVSNRFVGTERLLGSGHLVASSKP
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEECCCC
STIGRSTSGYVKFVDTLSSAVRVISYFIPSSFSSVWERAAIGRIIFYCVGLAVVAFLVCP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFIIIPISFSSSRFLDFPPPGLSLQWYEAYFTNGLWLSSTRQSLIVGFATAFVSLTVGTA
HHHHEECCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHH
AALALVRGSARMRRIGYQLLLAPMIVPSIVSAVAVYFLFSKLGLVDTTMGLVLAHSIGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
PLVVLIVAASLQSQNIRLEHAAASLGASRIVTIRKVVLPLIRPTLFVAGFFAFLHSFDEV
HHHHHHHHHHHCCCCEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSLFVSGPNTMTLPIKMWSGVREDITPTIAAVSSLLIGFTVILYGTIEVVRLRNKRKSK
HHHHHHCCCCEEEEEHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TMAKFNLALRGAKLAPNTSREAFVLLAPATVILGVCFFIPLAGLVALSFLSPEPTLMHY
CCCHHHHHEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KEFLGSWTSWVILGRTFSLALSVTSICLLLGYPLAYWIVSARAWVSGLLVFAVVLPFFTS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLVRSYAWMVLLGRFGIINQFLMKVGFTERPLDLIYNQFSVDVAMVYMLLPFTVMVLSSV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRGISPSLTRAASSLGAAPGQNFWRIFFPLSMPGVVASSLIVFVFALGFYITPALLGGPR
HHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NVTLPMLIDTYVNGTLNWPLAAAVATVLLLITLFLYTVSNRFVGTERLLGSGHLVASSKP
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEECCCC
STIGRSTSGYVKFVDTLSSAVRVISYFIPSSFSSVWERAAIGRIIFYCVGLAVVAFLVCP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFIIIPISFSSSRFLDFPPPGLSLQWYEAYFTNGLWLSSTRQSLIVGFATAFVSLTVGTA
HHHHEECCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHH
AALALVRGSARMRRIGYQLLLAPMIVPSIVSAVAVYFLFSKLGLVDTTMGLVLAHSIGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
PLVVLIVAASLQSQNIRLEHAAASLGASRIVTIRKVVLPLIRPTLFVAGFFAFLHSFDEV
HHHHHHHHHHHCCCCEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSLFVSGPNTMTLPIKMWSGVREDITPTIAAVSSLLIGFTVILYGTIEVVRLRNKRKSK
HHHHHHCCCCEEEEEHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]