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The map label for this gene is metE

Identifier: 13475114

GI number: 13475114

Start: 4954697

End: 4957027

Strand: Reverse

Name: metE

Synonym: mll6123

Alternate gene names: 13475114

Gene position: 4957027-4954697 (Counterclockwise)

Preceding gene: 13475115

Following gene: 13475113

Centisome position: 70.45

GC content: 62.16

Gene sequence:

>2331_bases
GTGGCAACGCTCGGCGTGCCGCGTATCGGTCGACGGCGGGAACTCAAATTCGCGCTTGAAAGCTACTGGTCCGGAAAATC
TCCCGCTGCCGACCTGCTCGCGACGGCGAAGGCGCTACGCGCCGCCAGCTGGAGGGAGCAGCACGATCGCGGCGTGTCGA
AAATCCCGTCCAACGATTTCTCGCTTTATGACCATGTGCTCGACACCGTCGCCATGGTCGGCGCCGTGCCGGCACGATAC
ACTTGGACTGACGGCGAGGTCCCACTCGATACCTATTTCGCAATGGCGCGCGGCAACCAAGATCAGGCCGCTGATTGCGG
ACATGCCGGTGACGAGCATGCGGCCAACGGGCATGGCCTTGCCGCGATGGAAATGACCAAATGGTTCGACACCAACTATC
ACTACATTGTGCCGGAACTTACCGACGATCAGAGCTTTGCCCTCTCGTCGGCGAAACCGGTCGATCATTTCCTCGAGGCC
AAGGCGCTCGGCATCCACACGCGCCCGGTCCTGCTTGGACCGGTTACCTTTCTCAAGTTGGCGAAGTCACCTGAGGAAGG
TTTCAACCCTATCGCGCTCCTGCCACGGCTGCTGCCCGTCTACGAGGAGCTCCTGCGGAGGCTGAAGCTCGCGGGAGCCG
ATTGGGTGCAGATCGATGAGCCGGCGCTCGTGGTCGATCTGGTTCCCAACGAACGGGATGCGCTCCAATTCGCCTACTGC
CGGCTGTCAAAAGCAGCACCGGAGTTGAAGATCATGGTCGCTATCTATTTCGGATCGCTGGGAGACAATCTCGAGACCGC
AATTTCTCTGCCCGTGGCGGGACTGCACGTCGATCTCGTGCGCGCTCCAGAGCAGTTGGAGACCGTCGGCCGGCTTGCGC
AGAAGGACCTTGTCCTCTCGCTCGGGCTGATCGACGGCCGCAACGTCTGGCGCGCGAACCTGCCTGCCATACTTGACCGC
ATCAAGCCGATCGTCGCCGGCTGGCCGCTGGACCGAGTGGAGATCGCCCCGTCCTGCTCGATGCTGCATGTGCCGATCGA
CCTGGACATGGAGACGGCCCTCGATGCGGACGTGAAATCGTGGCTCGCCTTCGCGGCGCAAAAAACCGACGAGCTGGTCG
TGCTCGCCCGTGCCCTGGCTGAAGGCCGGGACGCGGTCGCCGCCGAACTGGAGGCGTCGGCTGAGGCTGCCGCCGCTCGC
GCGACATCCGCTAAGGTGCATGATCCGCTCGTCGAAGGTCGGGTCGCCAGCATCAAGGTCGCCATGACGCGCCGGAAGAG
CGCATTCGACGTGCGCTCCAAGCTTCAGGGCGACACGTTTGGCTTGCCATCCTTCCCCACCACGACGATCGGCTCCTTCC
CGCAAACAGCCGAGGTTCGCAAGGCGCGGGCAGCCCATGCCAAGGGCGAGCTGAGTTACGTCGACTATGAGGCCTTCCTG
AAAAAAGAGATAGAAGCCGCCGTTCGCTGGCAAGAAGAGATCGGACTCGACGTGCTGGTCCACGGCGAATTCGAGCGCAA
CGATATGGTGCAGTATTTCGCCGAGCAGCTCCGCGGCTTTGCCTTCACGCAGCATGGGTGGGTGCAGAGCTATGGCTCGC
GCTTTGTGCGACCCCCGATCATCATTGGCGATGTGTCGCGACAAAATCCGATGACACTACACTGGTGGCGATACGCCCAG
TCGCTGACGCCAAAGCCGGTGAAGGGCATGCTTACCGGCCCGGTGACGATCCTCAACTGGTCCTTCGTCCGAGACGACCT
TCCGCGCTCAGCCGTCTGCCGCCAAGTCGCGCTCGCCATTCGCGATGAAGTGAGCGACCTCGAAAAGGCCGGCGCCAAGA
TGATCCAGATCGACGAAGCCGCACTGCGCGAGGGCTTGCCGCTGCGCGAGGCCGACTGGCAGGCCTATCTCGACTGGGCC
GTCGAATGCTTCCGGCTGGCTTCAACGGCGGTTGGCGATGCGACCCAGATTCACACCCATATGTGCTACTCCGAGTTCGG
CGACATCGTGGACGCGATTGCTGCAATGGATGCCGATGTGATCTCCATTGAAACCTCGCGCTCGCAGTTGGAACTGCTCG
ATACGCTGCGAACCTTCAAGTATCCGAACGAGATCGGTCCCGGCGTCTATGACATTCACTCGCCGCGGGTCCCGGAAGTG
GGCGAAATTTCCAACCTCATCATGCTTGCGCGCAAGCGATTGTCGGATGGCCAACTGTGGGTGAATCCCGACTGCGGTCT
CAAAACGCGCAGGTGGGAGGAGGTCCGCCCTGCCCTGGTGAACATGGTGGCTGCGGCACGCGCAATTCGGCAAAAAGCGC
AGACGGCGTAG

Upstream 100 bases:

>100_bases
GTGGCCGCGGTAGCCTGGCTTCGTGCCGCCTGCTCGCGCGTTCCTTGTCCCCACGCCCCAACCATCGGGGTATGGCATGA
CTGTCTCTCAACAAATTCCA

Downstream 100 bases:

>100_bases
GGCTCGGTGCCACTCGCCGGCGGTGAGCGGCAACGCCACACACCGGGCATCCTTGGGGGAGTCTATCCGGCGCGCCCAAG
GCAATCGAGCTGCCCGTGAC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MATLGVPRIGRRRELKFALESYWSGKSPAADLLATAKALRAASWREQHDRGVSKIPSNDFSLYDHVLDTVAMVGAVPARY
TWTDGEVPLDTYFAMARGNQDQAADCGHAGDEHAANGHGLAAMEMTKWFDTNYHYIVPELTDDQSFALSSAKPVDHFLEA
KALGIHTRPVLLGPVTFLKLAKSPEEGFNPIALLPRLLPVYEELLRRLKLAGADWVQIDEPALVVDLVPNERDALQFAYC
RLSKAAPELKIMVAIYFGSLGDNLETAISLPVAGLHVDLVRAPEQLETVGRLAQKDLVLSLGLIDGRNVWRANLPAILDR
IKPIVAGWPLDRVEIAPSCSMLHVPIDLDMETALDADVKSWLAFAAQKTDELVVLARALAEGRDAVAAELEASAEAAAAR
ATSAKVHDPLVEGRVASIKVAMTRRKSAFDVRSKLQGDTFGLPSFPTTTIGSFPQTAEVRKARAAHAKGELSYVDYEAFL
KKEIEAAVRWQEEIGLDVLVHGEFERNDMVQYFAEQLRGFAFTQHGWVQSYGSRFVRPPIIIGDVSRQNPMTLHWWRYAQ
SLTPKPVKGMLTGPVTILNWSFVRDDLPRSAVCRQVALAIRDEVSDLEKAGAKMIQIDEAALREGLPLREADWQAYLDWA
VECFRLASTAVGDATQIHTHMCYSEFGDIVDAIAAMDADVISIETSRSQLELLDTLRTFKYPNEIGPGVYDIHSPRVPEV
GEISNLIMLARKRLSDGQLWVNPDCGLKTRRWEEVRPALVNMVAAARAIRQKAQTA

Sequences:

>Translated_776_residues
MATLGVPRIGRRRELKFALESYWSGKSPAADLLATAKALRAASWREQHDRGVSKIPSNDFSLYDHVLDTVAMVGAVPARY
TWTDGEVPLDTYFAMARGNQDQAADCGHAGDEHAANGHGLAAMEMTKWFDTNYHYIVPELTDDQSFALSSAKPVDHFLEA
KALGIHTRPVLLGPVTFLKLAKSPEEGFNPIALLPRLLPVYEELLRRLKLAGADWVQIDEPALVVDLVPNERDALQFAYC
RLSKAAPELKIMVAIYFGSLGDNLETAISLPVAGLHVDLVRAPEQLETVGRLAQKDLVLSLGLIDGRNVWRANLPAILDR
IKPIVAGWPLDRVEIAPSCSMLHVPIDLDMETALDADVKSWLAFAAQKTDELVVLARALAEGRDAVAAELEASAEAAAAR
ATSAKVHDPLVEGRVASIKVAMTRRKSAFDVRSKLQGDTFGLPSFPTTTIGSFPQTAEVRKARAAHAKGELSYVDYEAFL
KKEIEAAVRWQEEIGLDVLVHGEFERNDMVQYFAEQLRGFAFTQHGWVQSYGSRFVRPPIIIGDVSRQNPMTLHWWRYAQ
SLTPKPVKGMLTGPVTILNWSFVRDDLPRSAVCRQVALAIRDEVSDLEKAGAKMIQIDEAALREGLPLREADWQAYLDWA
VECFRLASTAVGDATQIHTHMCYSEFGDIVDAIAAMDADVISIETSRSQLELLDTLRTFKYPNEIGPGVYDIHSPRVPEV
GEISNLIMLARKRLSDGQLWVNPDCGLKTRRWEEVRPALVNMVAAARAIRQKAQTA
>Mature_775_residues
ATLGVPRIGRRRELKFALESYWSGKSPAADLLATAKALRAASWREQHDRGVSKIPSNDFSLYDHVLDTVAMVGAVPARYT
WTDGEVPLDTYFAMARGNQDQAADCGHAGDEHAANGHGLAAMEMTKWFDTNYHYIVPELTDDQSFALSSAKPVDHFLEAK
ALGIHTRPVLLGPVTFLKLAKSPEEGFNPIALLPRLLPVYEELLRRLKLAGADWVQIDEPALVVDLVPNERDALQFAYCR
LSKAAPELKIMVAIYFGSLGDNLETAISLPVAGLHVDLVRAPEQLETVGRLAQKDLVLSLGLIDGRNVWRANLPAILDRI
KPIVAGWPLDRVEIAPSCSMLHVPIDLDMETALDADVKSWLAFAAQKTDELVVLARALAEGRDAVAAELEASAEAAAARA
TSAKVHDPLVEGRVASIKVAMTRRKSAFDVRSKLQGDTFGLPSFPTTTIGSFPQTAEVRKARAAHAKGELSYVDYEAFLK
KEIEAAVRWQEEIGLDVLVHGEFERNDMVQYFAEQLRGFAFTQHGWVQSYGSRFVRPPIIIGDVSRQNPMTLHWWRYAQS
LTPKPVKGMLTGPVTILNWSFVRDDLPRSAVCRQVALAIRDEVSDLEKAGAKMIQIDEAALREGLPLREADWQAYLDWAV
ECFRLASTAVGDATQIHTHMCYSEFGDIVDAIAAMDADVISIETSRSQLELLDTLRTFKYPNEIGPGVYDIHSPRVPEVG
EISNLIMLARKRLSDGQLWVNPDCGLKTRRWEEVRPALVNMVAAARAIRQKAQTA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=769, Percent_Identity=53.3159947984395, Blast_Score=785, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=778, Percent_Identity=49.7429305912596, Blast_Score=741, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_RHILO (Q98A73)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106678.1
- ProteinModelPortal:   Q98A73
- SMR:   Q98A73
- GeneID:   1229333
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll6123
- NMPDR:   fig|266835.1.peg.4779
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85820; Mature: 85689

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLGVPRIGRRRELKFALESYWSGKSPAADLLATAKALRAASWREQHDRGVSKIPSNDF
CCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCH
SLYDHVLDTVAMVGAVPARYTWTDGEVPLDTYFAMARGNQDQAADCGHAGDEHAANGHGL
HHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
AAMEMTKWFDTNYHYIVPELTDDQSFALSSAKPVDHFLEAKALGIHTRPVLLGPVTFLKL
EHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHH
AKSPEEGFNPIALLPRLLPVYEELLRRLKLAGADWVQIDEPALVVDLVPNERDALQFAYC
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHH
RLSKAAPELKIMVAIYFGSLGDNLETAISLPVAGLHVDLVRAPEQLETVGRLAQKDLVLS
HHHHCCCCEEEEEEEEECCCCCCCHHHHCCCCCCEEEHHHCCCHHHHHHHHHHHHHHEEE
LGLIDGRNVWRANLPAILDRIKPIVAGWPLDRVEIAPSCSMLHVPIDLDMETALDADVKS
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCHHHHHHHHHHH
WLAFAAQKTDELVVLARALAEGRDAVAAELEASAEAAAARATSAKVHDPLVEGRVASIKV
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCHHHCCCHHEEEE
AMTRRKSAFDVRSKLQGDTFGLPSFPTTTIGSFPQTAEVRKARAAHAKGELSYVDYEAFL
EHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECHHHHH
KKEIEAAVRWQEEIGLDVLVHGEFERNDMVQYFAEQLRGFAFTQHGWVQSYGSRFVRPPI
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCHHHHHHCCCCCCCCE
IIGDVSRQNPMTLHWWRYAQSLTPKPVKGMLTGPVTILNWSFVRDDLPRSAVCRQVALAI
EEECCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHHHH
RDEVSDLEKAGAKMIQIDEAALREGLPLREADWQAYLDWAVECFRLASTAVGDATQIHTH
HHHHHHHHHCCCEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MCYSEFGDIVDAIAAMDADVISIETSRSQLELLDTLRTFKYPNEIGPGVYDIHSPRVPEV
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCHHCCCCEEECCCCCCCCH
GEISNLIMLARKRLSDGQLWVNPDCGLKTRRWEEVRPALVNMVAAARAIRQKAQTA
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATLGVPRIGRRRELKFALESYWSGKSPAADLLATAKALRAASWREQHDRGVSKIPSNDF
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCH
SLYDHVLDTVAMVGAVPARYTWTDGEVPLDTYFAMARGNQDQAADCGHAGDEHAANGHGL
HHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
AAMEMTKWFDTNYHYIVPELTDDQSFALSSAKPVDHFLEAKALGIHTRPVLLGPVTFLKL
EHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHH
AKSPEEGFNPIALLPRLLPVYEELLRRLKLAGADWVQIDEPALVVDLVPNERDALQFAYC
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHH
RLSKAAPELKIMVAIYFGSLGDNLETAISLPVAGLHVDLVRAPEQLETVGRLAQKDLVLS
HHHHCCCCEEEEEEEEECCCCCCCHHHHCCCCCCEEEHHHCCCHHHHHHHHHHHHHHEEE
LGLIDGRNVWRANLPAILDRIKPIVAGWPLDRVEIAPSCSMLHVPIDLDMETALDADVKS
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCHHHHHHHHHHH
WLAFAAQKTDELVVLARALAEGRDAVAAELEASAEAAAARATSAKVHDPLVEGRVASIKV
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCHHHCCCHHEEEE
AMTRRKSAFDVRSKLQGDTFGLPSFPTTTIGSFPQTAEVRKARAAHAKGELSYVDYEAFL
EHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECHHHHH
KKEIEAAVRWQEEIGLDVLVHGEFERNDMVQYFAEQLRGFAFTQHGWVQSYGSRFVRPPI
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCHHHHHHCCCCCCCCE
IIGDVSRQNPMTLHWWRYAQSLTPKPVKGMLTGPVTILNWSFVRDDLPRSAVCRQVALAI
EEECCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHHHH
RDEVSDLEKAGAKMIQIDEAALREGLPLREADWQAYLDWAVECFRLASTAVGDATQIHTH
HHHHHHHHHCCCEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MCYSEFGDIVDAIAAMDADVISIETSRSQLELLDTLRTFKYPNEIGPGVYDIHSPRVPEV
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCHHCCCCEEECCCCCCCCH
GEISNLIMLARKRLSDGQLWVNPDCGLKTRRWEEVRPALVNMVAAARAIRQKAQTA
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968