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The map label for this gene is nac [H]

Identifier: 13475091

GI number: 13475091

Start: 4934227

End: 4935177

Strand: Direct

Name: nac [H]

Synonym: mlr6097

Alternate gene names: 13475091

Gene position: 4934227-4935177 (Clockwise)

Preceding gene: 13475090

Following gene: 13475092

Centisome position: 70.13

GC content: 58.57

Gene sequence:

>951_bases
ATGCCCCGGGTGGATTTCTTCATCGTGGACATCAGGCAGTTAAGATACTTTGTCGGCGTTGTCGAAGCCGGCAGTTTCAC
GAAGGCAGCTGCGACCCTCAATGTTGCTCAAAGCGCTTTAAGCCTGCATGTGCGTCAACTGGAGGAAGGATTCGGGACAC
AGCTGCTGGTTCGCGAGAGGACCGGTGTTAGTGTCACGGCGTCTGGAGACAAGCTGCTTGAGCGCGCGCGGAACATCCTT
AAGGAAATTCGACTCACCGAGGCGGAATTGACCAACACAGCCGCTTCCCCTGCCGGGGAGGTGACCATTGGCATTCCGTC
CGGAGCCGCTCGCGTCCTTAGCGGCCCCCTGCTTGAATCAGTGAGAGCCGAACTGCCGAGGGTCTCACTAAAGATGATCG
AGGGTATGACGGGACCGCTGGAGGAGTGGATGGCTGCTGGACGCTTCAATCTGGCAGTTCTCTACCGGACCGCGGACAGT
GTGGGGCGAATGACAGTCCTGGCGCGCGAAGACCTCTGTCTGGTGGTGCCGTCAGGTGAGCCGCCTTTCGAAGAGTCGAT
ATCTCTGGCGGACCTGCATGCGTTCCCGTTGGCGGTTCCCATGCGCAATAACAACGTCAGGCGTTCGGTGGCTGACGTCG
TCGCACAACACGGCTGCGCGCTGGATGTCAAGTTTGAAGTGGACTCGCTCTCAACCATCATCAACATGGTCATAGAGGGA
AAAGCCCATTCTATTCTCGCCCCCTCGGCCGTTCAAAAAGAGGCATCTCAAGGGCTGGTTAGGACGGTCAAAATCGTCGA
TCCGGTGATCACCCGTTCCGTCGTGCTTGCCGTAAATCCGAAAGATGAGCGTTCTGCGGCCGTCTCCGCGGTCCGCAAGC
TCATCCCCAAGGTCGCCAGAGAATTAATTGAGAGTCGGGCCTGGGCAGCTGCGGCACCTGACGCGGCCTGA

Upstream 100 bases:

>100_bases
TCAATCCTGAATCCCTTAAATCGCGACACGCTTTAGTGGGACAGTTTTGTGGCTTGCCTGCCATCTCGTTTCGAGATAGA
TATAAGAGCACTGGGGAAAC

Downstream 100 bases:

>100_bases
CAATCCATTCGACCTGTATGGCAACGCCACGACCTCTTGCGGCCATCAGGATCAGATCGATCTGGATTCCAGATGGACTT
CGGCAATATTCGATATTTGA

Product: nitrogen assimilation control protein

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPRVDFFIVDIRQLRYFVGVVEAGSFTKAAATLNVAQSALSLHVRQLEEGFGTQLLVRERTGVSVTASGDKLLERARNIL
KEIRLTEAELTNTAASPAGEVTIGIPSGAARVLSGPLLESVRAELPRVSLKMIEGMTGPLEEWMAAGRFNLAVLYRTADS
VGRMTVLAREDLCLVVPSGEPPFEESISLADLHAFPLAVPMRNNNVRRSVADVVAQHGCALDVKFEVDSLSTIINMVIEG
KAHSILAPSAVQKEASQGLVRTVKIVDPVITRSVVLAVNPKDERSAAVSAVRKLIPKVARELIESRAWAAAAPDAA

Sequences:

>Translated_316_residues
MPRVDFFIVDIRQLRYFVGVVEAGSFTKAAATLNVAQSALSLHVRQLEEGFGTQLLVRERTGVSVTASGDKLLERARNIL
KEIRLTEAELTNTAASPAGEVTIGIPSGAARVLSGPLLESVRAELPRVSLKMIEGMTGPLEEWMAAGRFNLAVLYRTADS
VGRMTVLAREDLCLVVPSGEPPFEESISLADLHAFPLAVPMRNNNVRRSVADVVAQHGCALDVKFEVDSLSTIINMVIEG
KAHSILAPSAVQKEASQGLVRTVKIVDPVITRSVVLAVNPKDERSAAVSAVRKLIPKVARELIESRAWAAAAPDAA
>Mature_315_residues
PRVDFFIVDIRQLRYFVGVVEAGSFTKAAATLNVAQSALSLHVRQLEEGFGTQLLVRERTGVSVTASGDKLLERARNILK
EIRLTEAELTNTAASPAGEVTIGIPSGAARVLSGPLLESVRAELPRVSLKMIEGMTGPLEEWMAAGRFNLAVLYRTADSV
GRMTVLAREDLCLVVPSGEPPFEESISLADLHAFPLAVPMRNNNVRRSVADVVAQHGCALDVKFEVDSLSTIINMVIEGK
AHSILAPSAVQKEASQGLVRTVKIVDPVITRSVVLAVNPKDERSAAVSAVRKLIPKVARELIESRAWAAAAPDAA

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=303, Percent_Identity=29.042904290429, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI157672245, Length=281, Percent_Identity=26.3345195729537, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790399, Length=274, Percent_Identity=25.1824817518248, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787601, Length=298, Percent_Identity=23.1543624161074, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787879, Length=294, Percent_Identity=28.2312925170068, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789506, Length=292, Percent_Identity=22.2602739726027, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI2367136, Length=130, Percent_Identity=32.3076923076923, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=125, Percent_Identity=34.4, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787806, Length=235, Percent_Identity=25.531914893617, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=269, Percent_Identity=25.2788104089219, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789204, Length=255, Percent_Identity=24.3137254901961, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788887, Length=189, Percent_Identity=30.6878306878307, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=168, Percent_Identity=27.9761904761905, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787589, Length=140, Percent_Identity=34.2857142857143, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33918; Mature: 33786

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRVDFFIVDIRQLRYFVGVVEAGSFTKAAATLNVAQSALSLHVRQLEEGFGTQLLVRER
CCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TGVSVTASGDKLLERARNILKEIRLTEAELTNTAASPAGEVTIGIPSGAARVLSGPLLES
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHHHHH
VRAELPRVSLKMIEGMTGPLEEWMAAGRFNLAVLYRTADSVGRMTVLAREDLCLVVPSGE
HHHHCCHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCCCCEEEEEECCEEEEECCCC
PPFEESISLADLHAFPLAVPMRNNNVRRSVADVVAQHGCALDVKFEVDSLSTIINMVIEG
CCHHHCCCHHHHHHCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCC
KAHSILAPSAVQKEASQGLVRTVKIVDPVITRSVVLAVNPKDERSAAVSAVRKLIPKVAR
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
ELIESRAWAAAAPDAA
HHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PRVDFFIVDIRQLRYFVGVVEAGSFTKAAATLNVAQSALSLHVRQLEEGFGTQLLVRER
CCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TGVSVTASGDKLLERARNILKEIRLTEAELTNTAASPAGEVTIGIPSGAARVLSGPLLES
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHHHHH
VRAELPRVSLKMIEGMTGPLEEWMAAGRFNLAVLYRTADSVGRMTVLAREDLCLVVPSGE
HHHHCCHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCCCCEEEEEECCEEEEECCCC
PPFEESISLADLHAFPLAVPMRNNNVRRSVADVVAQHGCALDVKFEVDSLSTIINMVIEG
CCHHHCCCHHHHHHCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCC
KAHSILAPSAVQKEASQGLVRTVKIVDPVITRSVVLAVNPKDERSAAVSAVRKLIPKVAR
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
ELIESRAWAAAAPDAA
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]