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The map label for this gene is appA [H]

Identifier: 13475076

GI number: 13475076

Start: 4918667

End: 4920385

Strand: Direct

Name: appA [H]

Synonym: mlr6082

Alternate gene names: 13475076

Gene position: 4918667-4920385 (Clockwise)

Preceding gene: 13475075

Following gene: 13475077

Centisome position: 69.91

GC content: 58.46

Gene sequence:

>1719_bases
ATGCGATTAGACACATATAATTTGAGGGGAGAAACTATGAACAAGTGGCCACTCAGTCGCCGCGCCGTAGCGAAAGCAAT
GCTTTTGGCATCGGCTGCACCTTTGGTCATGAATTTGGAGGCGCTTGCCGGCTCATCTTGCGCCGATGGCATCGTGCGTA
TCGCCGGAATTGCCGCACCCGACACGATGAATCCCTTTGCCACTTGGAGTTCGTCCTGGCCCATGTACTTCACCTATGAC
GCTCTGGTCGGCGTGGACGCCCAGCGCCACGACGATCGGAGGGGATTTGCCAAATCATGGTCCGTTGCCAAAGACAACCT
CACGTGGACATTCAAGATTTGGCCTAGCATGAAGTGGTCTGACGGCCAGCCTGCGACGGCGCGGGACGCTGCGTTCACTT
ATAACTATTTGCGGGGATCCATCGGCAAGCCGGACGAACTGAACGTTGGCTATAATAATACGGGTGGCTTGGAGAACATC
GAGTCCATTGCGGCACTGGATGACGAGACCCTGCAAATCGTGACCAAGAGCCGCACTCGTTGGCCTGTCGACAACTACAC
ATTGATGGTTCCCGAGCACATCTGGAAGGACGTATCTTACGCCGAAGCCCGCAGCACCTTCCAAAACAAGCCGCCGCTGG
TGGGAACCGGGCCTATGATCGTTTCAGAATTTCAACCCGGACAGTTTGCCAGTCTCACGCCTAACAAGTACTTCCGCGCG
GGGCGACCGGCGACGGCCGGCATGACCTACCGCTTCTTCAATTCTGCCGATCCTATCGCTCAGGGGTTGAAGAGCGGCGA
TCTTGATTACGGAATGAGCTTAACTGCTGCCCAGTGGGAGGAGCTGTCGAATGATCCTGCCATCGCTGTAGGAGAATCGC
GCATCGAGCAGCGGAATTATCTGGCCTTCAATACGCTCTCCGGGAAGGGTGCAGGCAGCACCAAGGCGCTCCAGGATCCG
GCGTTTCGCGATGCGATCGGCTACGCGATCGACCAGAAGACCATCGTCGATCGAGCCTTCCGCGGCCACGCCGACCCTGG
CGTCGGACTGGCCATGCCGGTGACGGCCGATTACTACTCCGAACTGAGTGAGATCAGGCGCCATTTCGATCTAGCCGAGG
CAGGGCGGCGACTTGATGCCGCCGGCTATCGGGATACCAACGGCGACGGTCTCCGTGAGGACCGGGATGGAAACCCGTTC
CAGCTGGAACTGATCACAGGGACCACGTCGGGTGTTCTTGAAATACCGACCGCGGCCATACAGCTTATCGCGGGCTGGCT
AGAGCAAATTGGCATACCTGTCACCGTAACTCAGCTCGACTCCGGCGCGCTCGCGGCCCGCACTGCAGCTCCGGCGGATG
GCGGCGGCGGGTGGGATCTGCTCGTCAGCGCAGGCTGGCTGGGTCCATCGGCTCACGACCTGCTCAGCTTGGGCAACAGT
AAGCTCATCGGCACCAGCAACACATCTTACTGGAAGAACGACAAGTTCGACAAGCTGCTGTCGGAAGCCGACGTCACCGT
TGACCTCAAGAAGAGTCGGGAATTAGTCGATCAAGCGTCGCGCTTGATTTACATCGAAGCGCCCTACATCATGCTCTGTT
ACCCCTTCCTGCTCGAAGCCCATCGAAAGGACTGCTTCCAAGGTTGGGGGACGAACGACGCGGTGTCGATGTGGGGCTAT
TTCCCGTTTGATCGCCTGAAGCCGGCGCCAACGCCGTGA

Upstream 100 bases:

>100_bases
GTGGGATCAAGCAACGGCAACTCCAATGGAAGCGCTACAGGGAAACCGCCGCTTTTTGGTTCAGGGATGACACGCGTGTC
GCGTCGCCAGCCTGTTCCAA

Downstream 100 bases:

>100_bases
ACGGAGGCGGGACGAGCCGGCATATTGGACGCATCATCATAAGGGCGATTGTGACGCTTCTGATGGTGGCGATACTTAAC
TTCATCCTGTTCCGAGTCAT

Product: ABC transporter, binding protein component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRLDTYNLRGETMNKWPLSRRAVAKAMLLASAAPLVMNLEALAGSSCADGIVRIAGIAAPDTMNPFATWSSSWPMYFTYD
ALVGVDAQRHDDRRGFAKSWSVAKDNLTWTFKIWPSMKWSDGQPATARDAAFTYNYLRGSIGKPDELNVGYNNTGGLENI
ESIAALDDETLQIVTKSRTRWPVDNYTLMVPEHIWKDVSYAEARSTFQNKPPLVGTGPMIVSEFQPGQFASLTPNKYFRA
GRPATAGMTYRFFNSADPIAQGLKSGDLDYGMSLTAAQWEELSNDPAIAVGESRIEQRNYLAFNTLSGKGAGSTKALQDP
AFRDAIGYAIDQKTIVDRAFRGHADPGVGLAMPVTADYYSELSEIRRHFDLAEAGRRLDAAGYRDTNGDGLREDRDGNPF
QLELITGTTSGVLEIPTAAIQLIAGWLEQIGIPVTVTQLDSGALAARTAAPADGGGGWDLLVSAGWLGPSAHDLLSLGNS
KLIGTSNTSYWKNDKFDKLLSEADVTVDLKKSRELVDQASRLIYIEAPYIMLCYPFLLEAHRKDCFQGWGTNDAVSMWGY
FPFDRLKPAPTP

Sequences:

>Translated_572_residues
MRLDTYNLRGETMNKWPLSRRAVAKAMLLASAAPLVMNLEALAGSSCADGIVRIAGIAAPDTMNPFATWSSSWPMYFTYD
ALVGVDAQRHDDRRGFAKSWSVAKDNLTWTFKIWPSMKWSDGQPATARDAAFTYNYLRGSIGKPDELNVGYNNTGGLENI
ESIAALDDETLQIVTKSRTRWPVDNYTLMVPEHIWKDVSYAEARSTFQNKPPLVGTGPMIVSEFQPGQFASLTPNKYFRA
GRPATAGMTYRFFNSADPIAQGLKSGDLDYGMSLTAAQWEELSNDPAIAVGESRIEQRNYLAFNTLSGKGAGSTKALQDP
AFRDAIGYAIDQKTIVDRAFRGHADPGVGLAMPVTADYYSELSEIRRHFDLAEAGRRLDAAGYRDTNGDGLREDRDGNPF
QLELITGTTSGVLEIPTAAIQLIAGWLEQIGIPVTVTQLDSGALAARTAAPADGGGGWDLLVSAGWLGPSAHDLLSLGNS
KLIGTSNTSYWKNDKFDKLLSEADVTVDLKKSRELVDQASRLIYIEAPYIMLCYPFLLEAHRKDCFQGWGTNDAVSMWGY
FPFDRLKPAPTP
>Mature_572_residues
MRLDTYNLRGETMNKWPLSRRAVAKAMLLASAAPLVMNLEALAGSSCADGIVRIAGIAAPDTMNPFATWSSSWPMYFTYD
ALVGVDAQRHDDRRGFAKSWSVAKDNLTWTFKIWPSMKWSDGQPATARDAAFTYNYLRGSIGKPDELNVGYNNTGGLENI
ESIAALDDETLQIVTKSRTRWPVDNYTLMVPEHIWKDVSYAEARSTFQNKPPLVGTGPMIVSEFQPGQFASLTPNKYFRA
GRPATAGMTYRFFNSADPIAQGLKSGDLDYGMSLTAAQWEELSNDPAIAVGESRIEQRNYLAFNTLSGKGAGSTKALQDP
AFRDAIGYAIDQKTIVDRAFRGHADPGVGLAMPVTADYYSELSEIRRHFDLAEAGRRLDAAGYRDTNGDGLREDRDGNPF
QLELITGTTSGVLEIPTAAIQLIAGWLEQIGIPVTVTQLDSGALAARTAAPADGGGGWDLLVSAGWLGPSAHDLLSLGNS
KLIGTSNTSYWKNDKFDKLLSEADVTVDLKKSRELVDQASRLIYIEAPYIMLCYPFLLEAHRKDCFQGWGTNDAVSMWGY
FPFDRLKPAPTP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=481, Percent_Identity=23.4927234927235, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1787052, Length=481, Percent_Identity=24.3243243243243, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787551, Length=489, Percent_Identity=22.9038854805726, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62699; Mature: 62699

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDTYNLRGETMNKWPLSRRAVAKAMLLASAAPLVMNLEALAGSSCADGIVRIAGIAAP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEHHHHCCCCHHHHHEEEEEECCC
DTMNPFATWSSSWPMYFTYDALVGVDAQRHDDRRGFAKSWSVAKDNLTWTFKIWPSMKWS
CCCCCCCCCCCCCCEEEEEHHHCCCCCHHCCCCCCHHHCCCEECCCCEEEEEECCCCCCC
DGQPATARDAAFTYNYLRGSIGKPDELNVGYNNTGGLENIESIAALDDETLQIVTKSRTR
CCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCC
WPVDNYTLMVPEHIWKDVSYAEARSTFQNKPPLVGTGPMIVSEFQPGQFASLTPNKYFRA
CCCCCEEEEECHHHHHCCHHHHHHHHHCCCCCCEECCCEEEECCCCCCEECCCCHHHHCC
GRPATAGMTYRFFNSADPIAQGLKSGDLDYGMSLTAAQWEELSNDPAIAVGESRIEQRNY
CCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCEECHHHHHHCCCCCCEEECHHHHHHCCE
LAFNTLSGKGAGSTKALQDPAFRDAIGYAIDQKTIVDRAFRGHADPGVGLAMPVTADYYS
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEECCCHHHHH
ELSEIRRHFDLAEAGRRLDAAGYRDTNGDGLREDRDGNPFQLELITGTTSGVLEIPTAAI
HHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCHHHH
QLIAGWLEQIGIPVTVTQLDSGALAARTAAPADGGGGWDLLVSAGWLGPSAHDLLSLGNS
HHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCC
KLIGTSNTSYWKNDKFDKLLSEADVTVDLKKSRELVDQASRLIYIEAPYIMLCYPFLLEA
EEEECCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
HRKDCFQGWGTNDAVSMWGYFPFDRLKPAPTP
HHHHHHHCCCCCCHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure
MRLDTYNLRGETMNKWPLSRRAVAKAMLLASAAPLVMNLEALAGSSCADGIVRIAGIAAP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEHHHHCCCCHHHHHEEEEEECCC
DTMNPFATWSSSWPMYFTYDALVGVDAQRHDDRRGFAKSWSVAKDNLTWTFKIWPSMKWS
CCCCCCCCCCCCCCEEEEEHHHCCCCCHHCCCCCCHHHCCCEECCCCEEEEEECCCCCCC
DGQPATARDAAFTYNYLRGSIGKPDELNVGYNNTGGLENIESIAALDDETLQIVTKSRTR
CCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCC
WPVDNYTLMVPEHIWKDVSYAEARSTFQNKPPLVGTGPMIVSEFQPGQFASLTPNKYFRA
CCCCCEEEEECHHHHHCCHHHHHHHHHCCCCCCEECCCEEEECCCCCCEECCCCHHHHCC
GRPATAGMTYRFFNSADPIAQGLKSGDLDYGMSLTAAQWEELSNDPAIAVGESRIEQRNY
CCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCEECHHHHHHCCCCCCEEECHHHHHHCCE
LAFNTLSGKGAGSTKALQDPAFRDAIGYAIDQKTIVDRAFRGHADPGVGLAMPVTADYYS
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEECCCHHHHH
ELSEIRRHFDLAEAGRRLDAAGYRDTNGDGLREDRDGNPFQLELITGTTSGVLEIPTAAI
HHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCHHHH
QLIAGWLEQIGIPVTVTQLDSGALAARTAAPADGGGGWDLLVSAGWLGPSAHDLLSLGNS
HHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCC
KLIGTSNTSYWKNDKFDKLLSEADVTVDLKKSRELVDQASRLIYIEAPYIMLCYPFLLEA
EEEECCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
HRKDCFQGWGTNDAVSMWGYFPFDRLKPAPTP
HHHHHHHCCCCCCHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]