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The map label for this gene is nifA [H]

Identifier: 13474861

GI number: 13474861

Start: 4710342

End: 4712165

Strand: Reverse

Name: nifA [H]

Synonym: mll5837

Alternate gene names: 13474861

Gene position: 4712165-4710342 (Counterclockwise)

Preceding gene: 13474862

Following gene: 13474860

Centisome position: 66.97

GC content: 59.16

Gene sequence:

>1824_bases
ATGGATGATCACATGCACGATACTCGCAACTCCGAGCAAAGTGCCAGGCAGATCTCGTCACGCTGCATATCGCTTCCAAA
AATGAAAAGCCCTAATTCAAAGTCCGAGATGAATAACGTGGTCCCTATTGTGCCGTTGCACGAGAGGGCGCCGGACATCT
TTGAAACGTCGATAGCGCTCACCGCTCCCTCTGGGCTGGAAGCCACGTTGGCCAACGTCGTCGACCTCCTGCCATCGTTG
GTGCAGGTGCGGCACGGCATCATCTCGCTCTGCGACGGTGCCGGCATACCGGACATGACCGTCGGCGCCGGCTGGAGCGA
AGGCAGCGATGAGCGCTACCGCAAGCATCTGCCACGGGCGGCGATCGACCAGATCATAACGACGGGCATGGCGCTCGTCG
CCGAGAACATAGCGTTGGATCCGGCTTTCAACGCCGCCGACAAGGAGGTGCTCGGGGCGTCCGACAACATGAAAGTGTCG
TTCATCGGCGTACCCATTCGCGTCGATGCGAACGTCGTGGGCACGCTGACCATCGACCGCATCCTGGACAACAAATCAGG
CTCCCTCCTCGATTACGACCTGCGCCTCCTTACCACAATCGCTAATCTGGTTGGACAGACAGTGAAGCTGCATCGCCTGT
TCGCGTCCGACCGCGAGCGACTGATGGCCGAAAAAATCCGGATGCAAAAGCAATTCGGCGAACTCAAGCAGCCTGGGCAT
GAGGGCAAAAGGGCTCATGTCAAGGGGATCATTGGCGACAGCCCGGCGCTGCGCGGGCTGCTTGAGAAGGTCGCGTTAGT
AGCCAGGTCCAACAGCACCGTTTTGCTGCGCGGGGAATCGGGGACCGGCAAGGAGCTGGTCGCCAAGGCCGTTCACGAGA
TGTCGGGACGCGCCAAGCGGCCGTTCATCAAGCTCAATTGCGCAGCGCTCCCTGAGACAGTCCTGGAATCCGAACTGTTC
GGTCACGAAAAGGGTGCCTTTACCAGTGCATTCAACTCGCGCAAGGGGCGCTTTGAGCTCGCCGACAAGGGAACGTTGTT
TCTGGACGAGATCGGTGAGATTTCGGCCTCGTTCCAGGCAAAGCTGCTGCGCGTGCTGCAGGAGCAGGAGTTCGAGCGCG
TCGGCAGCAACCAGACGATTAAGGTCGACGTTCGCGTGATTGCCGCCACGAACAGGAACCTGGAAGACGCGGTTGCAAGG
AACGAGTTTCGCGCCGACCTTTATTATCGCATCAGCGTTGTTCCCTTGCTGGTGCCGCCATTGCGCGAAAGGCGCGGTGA
TATTCCGCTCCTTGCTGCTGAGTTTCTCAAGAATTTCAACAGCGAGAACGGCCGTACGATGGTCTTCGATGCGAGTGCGA
CTGAAGTGCTGATGAACTGTGCTTTTCCCGGAAATGTCCGCGAGCTTGAAAATTGCGTGCAGCGGACAGCGACCCTGGCA
GCGGGAGCGTCAATCGGCAGAAACGACTTTGACTGCTGCCATGGCCGATGCCTTTCCGCGATGCTATGGAAGCACGCATC
GAAGGAGACTGCGCCGAAATCGGAGCCGATCGCACCATCGCCACTGAACCCGGCCATGCAATCGTCTGGTGCTTTTGCTT
CGGCCGCCATTGGGGTCCCGCATGACGACAGCGAGCAGGCACTGCCAGCGCCTGTTCGGCTAGGCCTCGTAAGCGACGCG
AAGATGACCGATCGCGAGCGTCTCATCGCGGCCATGGAAAGATCCGGCTGGGTACAGGCAAAGGCAGCGCGCCTGCTTGG
ACTGACGCCGCGCCAGATTGGTTACGCGCTGAGGAAATACGGTATCGAGATCAAGAATCTCTGA

Upstream 100 bases:

>100_bases
GGCCTGCGGAGCGAAGACGCCGACGAATGCGATTCGCCAAGGACATCCACGCGCTGGACAATAGGCGCACTTGCCTCGGG
CATCGATTTCATGGCGCGAG

Downstream 100 bases:

>100_bases
ACCTGCTGTCGGATTTCAAGCGGGGCCACGAGATAGCGCGCGAGGCGAATAGCTGGGACAAATTAGCGTTGATTGGTACG
GCTCGGACCTCCGTCGGTGA

Product: NIFA, nif-specific regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MDDHMHDTRNSEQSARQISSRCISLPKMKSPNSKSEMNNVVPIVPLHERAPDIFETSIALTAPSGLEATLANVVDLLPSL
VQVRHGIISLCDGAGIPDMTVGAGWSEGSDERYRKHLPRAAIDQIITTGMALVAENIALDPAFNAADKEVLGASDNMKVS
FIGVPIRVDANVVGTLTIDRILDNKSGSLLDYDLRLLTTIANLVGQTVKLHRLFASDRERLMAEKIRMQKQFGELKQPGH
EGKRAHVKGIIGDSPALRGLLEKVALVARSNSTVLLRGESGTGKELVAKAVHEMSGRAKRPFIKLNCAALPETVLESELF
GHEKGAFTSAFNSRKGRFELADKGTLFLDEIGEISASFQAKLLRVLQEQEFERVGSNQTIKVDVRVIAATNRNLEDAVAR
NEFRADLYYRISVVPLLVPPLRERRGDIPLLAAEFLKNFNSENGRTMVFDASATEVLMNCAFPGNVRELENCVQRTATLA
AGASIGRNDFDCCHGRCLSAMLWKHASKETAPKSEPIAPSPLNPAMQSSGAFASAAIGVPHDDSEQALPAPVRLGLVSDA
KMTDRERLIAAMERSGWVQAKAARLLGLTPRQIGYALRKYGIEIKNL

Sequences:

>Translated_607_residues
MDDHMHDTRNSEQSARQISSRCISLPKMKSPNSKSEMNNVVPIVPLHERAPDIFETSIALTAPSGLEATLANVVDLLPSL
VQVRHGIISLCDGAGIPDMTVGAGWSEGSDERYRKHLPRAAIDQIITTGMALVAENIALDPAFNAADKEVLGASDNMKVS
FIGVPIRVDANVVGTLTIDRILDNKSGSLLDYDLRLLTTIANLVGQTVKLHRLFASDRERLMAEKIRMQKQFGELKQPGH
EGKRAHVKGIIGDSPALRGLLEKVALVARSNSTVLLRGESGTGKELVAKAVHEMSGRAKRPFIKLNCAALPETVLESELF
GHEKGAFTSAFNSRKGRFELADKGTLFLDEIGEISASFQAKLLRVLQEQEFERVGSNQTIKVDVRVIAATNRNLEDAVAR
NEFRADLYYRISVVPLLVPPLRERRGDIPLLAAEFLKNFNSENGRTMVFDASATEVLMNCAFPGNVRELENCVQRTATLA
AGASIGRNDFDCCHGRCLSAMLWKHASKETAPKSEPIAPSPLNPAMQSSGAFASAAIGVPHDDSEQALPAPVRLGLVSDA
KMTDRERLIAAMERSGWVQAKAARLLGLTPRQIGYALRKYGIEIKNL
>Mature_607_residues
MDDHMHDTRNSEQSARQISSRCISLPKMKSPNSKSEMNNVVPIVPLHERAPDIFETSIALTAPSGLEATLANVVDLLPSL
VQVRHGIISLCDGAGIPDMTVGAGWSEGSDERYRKHLPRAAIDQIITTGMALVAENIALDPAFNAADKEVLGASDNMKVS
FIGVPIRVDANVVGTLTIDRILDNKSGSLLDYDLRLLTTIANLVGQTVKLHRLFASDRERLMAEKIRMQKQFGELKQPGH
EGKRAHVKGIIGDSPALRGLLEKVALVARSNSTVLLRGESGTGKELVAKAVHEMSGRAKRPFIKLNCAALPETVLESELF
GHEKGAFTSAFNSRKGRFELADKGTLFLDEIGEISASFQAKLLRVLQEQEFERVGSNQTIKVDVRVIAATNRNLEDAVAR
NEFRADLYYRISVVPLLVPPLRERRGDIPLLAAEFLKNFNSENGRTMVFDASATEVLMNCAFPGNVRELENCVQRTATLA
AGASIGRNDFDCCHGRCLSAMLWKHASKETAPKSEPIAPSPLNPAMQSSGAFASAAIGVPHDDSEQALPAPVRLGLVSDA
KMTDRERLIAAMERSGWVQAKAARLLGLTPRQIGYALRKYGIEIKNL

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=355, Percent_Identity=42.2535211267606, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1789087, Length=298, Percent_Identity=50, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1790437, Length=346, Percent_Identity=44.5086705202312, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI87082117, Length=243, Percent_Identity=53.9094650205761, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082152, Length=238, Percent_Identity=55.0420168067227, Blast_Score=256, Evalue=4e-69,
Organism=Escherichia coli, GI1790299, Length=379, Percent_Identity=40.1055408970976, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=50, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1789233, Length=231, Percent_Identity=46.3203463203463, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=46.7213114754098, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081872, Length=355, Percent_Identity=37.4647887323944, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787583, Length=241, Percent_Identity=42.7385892116183, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789828, Length=252, Percent_Identity=37.6984126984127, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI87081858, Length=225, Percent_Identity=36.4444444444444, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 66147; Mature: 66147

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDHMHDTRNSEQSARQISSRCISLPKMKSPNSKSEMNNVVPIVPLHERAPDIFETSIAL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEECCCCCCCHHHCCEEE
TAPSGLEATLANVVDLLPSLVQVRHGIISLCDGAGIPDMTVGAGWSEGSDERYRKHLPRA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHCCHH
AIDQIITTGMALVAENIALDPAFNAADKEVLGASDNMKVSFIGVPIRVDANVVGTLTIDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEEEEEECCCEEEEEEHHH
ILDNKSGSLLDYDLRLLTTIANLVGQTVKLHRLFASDRERLMAEKIRMQKQFGELKQPGH
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGKRAHVKGIIGDSPALRGLLEKVALVARSNSTVLLRGESGTGKELVAKAVHEMSGRAKR
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
PFIKLNCAALPETVLESELFGHEKGAFTSAFNSRKGRFELADKGTLFLDEIGEISASFQA
CEEEEEHHHCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHHH
KLLRVLQEQEFERVGSNQTIKVDVRVIAATNRNLEDAVARNEFRADLYYRISVVPLLVPP
HHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCEEEEEEEHHHHCCC
LRERRGDIPLLAAEFLKNFNSENGRTMVFDASATEVLMNCAFPGNVRELENCVQRTATLA
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AGASIGRNDFDCCHGRCLSAMLWKHASKETAPKSEPIAPSPLNPAMQSSGAFASAAIGVP
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCC
HDDSEQALPAPVRLGLVSDAKMTDRERLIAAMERSGWVQAKAARLLGLTPRQIGYALRKY
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
GIEIKNL
CCEECCC
>Mature Secondary Structure
MDDHMHDTRNSEQSARQISSRCISLPKMKSPNSKSEMNNVVPIVPLHERAPDIFETSIAL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEECCCCCCCHHHCCEEE
TAPSGLEATLANVVDLLPSLVQVRHGIISLCDGAGIPDMTVGAGWSEGSDERYRKHLPRA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHCCHH
AIDQIITTGMALVAENIALDPAFNAADKEVLGASDNMKVSFIGVPIRVDANVVGTLTIDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEEEEEECCCEEEEEEHHH
ILDNKSGSLLDYDLRLLTTIANLVGQTVKLHRLFASDRERLMAEKIRMQKQFGELKQPGH
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGKRAHVKGIIGDSPALRGLLEKVALVARSNSTVLLRGESGTGKELVAKAVHEMSGRAKR
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
PFIKLNCAALPETVLESELFGHEKGAFTSAFNSRKGRFELADKGTLFLDEIGEISASFQA
CEEEEEHHHCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHHH
KLLRVLQEQEFERVGSNQTIKVDVRVIAATNRNLEDAVARNEFRADLYYRISVVPLLVPP
HHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCEEEEEEEHHHHCCC
LRERRGDIPLLAAEFLKNFNSENGRTMVFDASATEVLMNCAFPGNVRELENCVQRTATLA
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AGASIGRNDFDCCHGRCLSAMLWKHASKETAPKSEPIAPSPLNPAMQSSGAFASAAIGVP
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCC
HDDSEQALPAPVRLGLVSDAKMTDRERLIAAMERSGWVQAKAARLLGLTPRQIGYALRKY
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
GIEIKNL
CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3313281; 12597275; 3357773; 2792368 [H]