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The map label for this gene is nadB

Identifier: 13474858

GI number: 13474858

Start: 4706229

End: 4707770

Strand: Reverse

Name: nadB

Synonym: mll5834

Alternate gene names: 13474858

Gene position: 4707770-4706229 (Counterclockwise)

Preceding gene: 13474859

Following gene: 13474857

Centisome position: 66.91

GC content: 64.07

Gene sequence:

>1542_bases
ATGACGGCGGACATCCATCACATTGGCGGAGCGCCGGTCATTATCGGCGCTGGTATCGCCGGGCTCATGACGGCGCTACA
CTTGGCGCCGCAACCAGTCGTCCTTCTGTCAAGGACCTCGCTCGGAACCGAAGCCTCGAGCATTCTAGCCCAGGGCGGGC
TCGCAGCGAGTCTGGGGGAGGACGACAGCCCCGACCTGCACCTCGCCGATACGCTTGCTGCCGGCGAAGGGCTTTGTGAC
GAACAAATGGCCAGGCGGGTGGTAGAGGCCGCACCTCAAGCCGTCGAGAATCTCATTCGTCTTGGTACTCCTTTCGACCG
CTCGCTAGACGGGAGGCTGCAGCTGGGCCTGGAAGCTGCGCATTCGCGTCGCCGCATCGTGCACGCCGCCGGCGACGCAA
CCGGCCGCGAGTTGTTTCGGGCGCTACTCGGCGTGGCGCGGCGAACCCGTTCGATCACGATCATGGAAGGCATGACAGCG
CTTCGCTTGGTTGTCGCAGAGGGCAGCATTGTTGGCTTGCTGACTGTCCTGCATGGCGCCGTGTTCGCATTGCCCACACG
CCGCGTGGTGCTGGCGACCGGTGGTATAGGCGGCCTGTTTTGCGATACAACCAATCCGCTCTCCTCATTCGGGCACGGCC
TGGCGCTTGCAGCCTGTGCTGGAGCGGAACTAGCCGATCTCGAATTCGTGCAATTCCACCCGACGGCCCTCGACATTGCG
CGCCGGCCAATGCCGCTTGTCAGCGAAGCAGTGCGTGGCGAAGGCGCGGTGCTGATCGATGAGCACGGCCATCGCCTCCT
GGCAGACACGCCCGGGGCAGAACTCGCATCGCGCGATGTGGTCGCGCGGGCGATCTCCGATCAGCTCGCAGCCGGGCATG
GCGTCTATCTCGATGCCCGACATTGTCTCGGGCAGAAATTCGCAAGACGCTTTCCGGCAATCGACGCTATTTGCAAGCAT
GCCGGCATCGATCCGGCGGTGGAACCCATTCCCGTACGGCCCGCTGCTCACTATCACATGGGCGGCGTGGCCGTTGACGC
CGAGGGGCGAACTTCCGTTAGCGGCCTGTGGGCCTGTGGCGAAGTCGCATGCACAGGACTGCATGGCGCCAACCGGCTTG
CCAGCAACTCGCTGACCGAAGCGGTTGCAACCGCCGCCTGGGTGGCTGAAAGCGTCGCGGGTACCTCTGCGGGTTGGCAG
TGGCCTAGGCTTCCGGCAACTGTTCCCATCCGGCCCGACCCTTCACCTATTCGCACGATTGTGTCCAATGCGCTTGGCAT
TGTTCGGAATGGGAAATCATTGTGCGACACGGTCGCGACACTGCTGCCGATCTCTGTTTGCGAGACGGCCGCGGCCGATC
CGGCCCTGGTGGCATTGTTGATGGCGATCGCCGCCCTTCGGCGCGAGGAGAGCCGCGGCTCCCACTTTCGATCCGATTTC
CCCGGGCGCGATGCCGCCGCCCTCCCCTCACGATTGACGCTCTGCACAGCTTTTGAGAATGCCGTCACGCTCAGATGGCA
AGCATCCGAACGGAGCCGTTGA

Upstream 100 bases:

>100_bases
CCAACATCCGGACCGCGCTCGAGGAGAACCGCCACGTCGTCACGATCGATCCTCATGTCGCTGAACGGGCCCGCTGGGCT
GTGGAACGCATGCTCTTTGT

Downstream 100 bases:

>100_bases
CATGACTTCACTTGCACCACTTCCCCAGATCATCATGGAGCCCATCGTGCGTTCTGCCCTGCTTGAAGACCTGGGCAGAG
CCGGCGACATCACGAGCGAT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTADIHHIGGAPVIIGAGIAGLMTALHLAPQPVVLLSRTSLGTEASSILAQGGLAASLGEDDSPDLHLADTLAAGEGLCD
EQMARRVVEAAPQAVENLIRLGTPFDRSLDGRLQLGLEAAHSRRRIVHAAGDATGRELFRALLGVARRTRSITIMEGMTA
LRLVVAEGSIVGLLTVLHGAVFALPTRRVVLATGGIGGLFCDTTNPLSSFGHGLALAACAGAELADLEFVQFHPTALDIA
RRPMPLVSEAVRGEGAVLIDEHGHRLLADTPGAELASRDVVARAISDQLAAGHGVYLDARHCLGQKFARRFPAIDAICKH
AGIDPAVEPIPVRPAAHYHMGGVAVDAEGRTSVSGLWACGEVACTGLHGANRLASNSLTEAVATAAWVAESVAGTSAGWQ
WPRLPATVPIRPDPSPIRTIVSNALGIVRNGKSLCDTVATLLPISVCETAAADPALVALLMAIAALRREESRGSHFRSDF
PGRDAAALPSRLTLCTAFENAVTLRWQASERSR

Sequences:

>Translated_513_residues
MTADIHHIGGAPVIIGAGIAGLMTALHLAPQPVVLLSRTSLGTEASSILAQGGLAASLGEDDSPDLHLADTLAAGEGLCD
EQMARRVVEAAPQAVENLIRLGTPFDRSLDGRLQLGLEAAHSRRRIVHAAGDATGRELFRALLGVARRTRSITIMEGMTA
LRLVVAEGSIVGLLTVLHGAVFALPTRRVVLATGGIGGLFCDTTNPLSSFGHGLALAACAGAELADLEFVQFHPTALDIA
RRPMPLVSEAVRGEGAVLIDEHGHRLLADTPGAELASRDVVARAISDQLAAGHGVYLDARHCLGQKFARRFPAIDAICKH
AGIDPAVEPIPVRPAAHYHMGGVAVDAEGRTSVSGLWACGEVACTGLHGANRLASNSLTEAVATAAWVAESVAGTSAGWQ
WPRLPATVPIRPDPSPIRTIVSNALGIVRNGKSLCDTVATLLPISVCETAAADPALVALLMAIAALRREESRGSHFRSDF
PGRDAAALPSRLTLCTAFENAVTLRWQASERSR
>Mature_512_residues
TADIHHIGGAPVIIGAGIAGLMTALHLAPQPVVLLSRTSLGTEASSILAQGGLAASLGEDDSPDLHLADTLAAGEGLCDE
QMARRVVEAAPQAVENLIRLGTPFDRSLDGRLQLGLEAAHSRRRIVHAAGDATGRELFRALLGVARRTRSITIMEGMTAL
RLVVAEGSIVGLLTVLHGAVFALPTRRVVLATGGIGGLFCDTTNPLSSFGHGLALAACAGAELADLEFVQFHPTALDIAR
RPMPLVSEAVRGEGAVLIDEHGHRLLADTPGAELASRDVVARAISDQLAAGHGVYLDARHCLGQKFARRFPAIDAICKHA
GIDPAVEPIPVRPAAHYHMGGVAVDAEGRTSVSGLWACGEVACTGLHGANRLASNSLTEAVATAAWVAESVAGTSAGWQW
PRLPATVPIRPDPSPIRTIVSNALGIVRNGKSLCDTVATLLPISVCETAAADPALVALLMAIAALRREESRGSHFRSDFP
GRDAAALPSRLTLCTAFENAVTLRWQASERSR

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=518, Percent_Identity=31.0810810810811, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI1788928, Length=529, Percent_Identity=37.9962192816635, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1790597, Length=362, Percent_Identity=35.0828729281768, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786942, Length=528, Percent_Identity=30.3030303030303, Blast_Score=178, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=511, Percent_Identity=32.2896281800391, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17505833, Length=512, Percent_Identity=30.2734375, Blast_Score=181, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322701, Length=520, Percent_Identity=28.6538461538462, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322416, Length=520, Percent_Identity=30.9615384615385, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6322511, Length=477, Percent_Identity=25.7861635220126, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320788, Length=482, Percent_Identity=25.3112033195021, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137288, Length=511, Percent_Identity=30.9197651663405, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24655642, Length=511, Percent_Identity=30.9197651663405, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24655647, Length=511, Percent_Identity=30.9197651663405, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24663005, Length=512, Percent_Identity=28.90625, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_RHILO (Q98AV8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106428.1
- ProteinModelPortal:   Q98AV8
- GeneID:   1229087
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5834
- NMPDR:   fig|266835.1.peg.4531
- HOGENOM:   HBG293998
- OMA:   RSITIME
- ProtClustDB:   PRK07512
- BRENDA:   1.4.3.16
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00551

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 53470; Mature: 53339

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: ACT_SITE 233-233 ACT_SITE 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADIHHIGGAPVIIGAGIAGLMTALHLAPQPVVLLSRTSLGTEASSILAQGGLAASLGE
CCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCEECCCC
DDSPDLHLADTLAAGEGLCDEQMARRVVEAAPQAVENLIRLGTPFDRSLDGRLQLGLEAA
CCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHH
HSRRRIVHAAGDATGRELFRALLGVARRTRSITIMEGMTALRLVVAEGSIVGLLTVLHGA
HHHHHEEEECCCCCHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFALPTRRVVLATGGIGGLFCDTTNPLSSFGHGLALAACAGAELADLEFVQFHPTALDIA
HHHCCCCEEEEEECCCCEEEECCCCHHHHHCCHHHHHHHCCCCCCCCEEEEECCHHHHHH
RRPMPLVSEAVRGEGAVLIDEHGHRLLADTPGAELASRDVVARAISDQLAAGHGVYLDAR
CCCCHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHH
HCLGQKFARRFPAIDAICKHAGIDPAVEPIPVRPAAHYHMGGVAVDAEGRTSVSGLWACG
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEECCCCCCCCHHHHHHH
EVACTGLHGANRLASNSLTEAVATAAWVAESVAGTSAGWQWPRLPATVPIRPDPSPIRTI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHH
VSNALGIVRNGKSLCDTVATLLPISVCETAAADPALVALLMAIAALRREESRGSHFRSDF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGRDAAALPSRLTLCTAFENAVTLRWQASERSR
CCCCHHHCCHHHHHHEECCCEEEEEEECCCCCC
>Mature Secondary Structure 
TADIHHIGGAPVIIGAGIAGLMTALHLAPQPVVLLSRTSLGTEASSILAQGGLAASLGE
CCCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCEECCCC
DDSPDLHLADTLAAGEGLCDEQMARRVVEAAPQAVENLIRLGTPFDRSLDGRLQLGLEAA
CCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHH
HSRRRIVHAAGDATGRELFRALLGVARRTRSITIMEGMTALRLVVAEGSIVGLLTVLHGA
HHHHHEEEECCCCCHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFALPTRRVVLATGGIGGLFCDTTNPLSSFGHGLALAACAGAELADLEFVQFHPTALDIA
HHHCCCCEEEEEECCCCEEEECCCCHHHHHCCHHHHHHHCCCCCCCCEEEEECCHHHHHH
RRPMPLVSEAVRGEGAVLIDEHGHRLLADTPGAELASRDVVARAISDQLAAGHGVYLDAR
CCCCHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHH
HCLGQKFARRFPAIDAICKHAGIDPAVEPIPVRPAAHYHMGGVAVDAEGRTSVSGLWACG
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEECCCCCCCCHHHHHHH
EVACTGLHGANRLASNSLTEAVATAAWVAESVAGTSAGWQWPRLPATVPIRPDPSPIRTI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHH
VSNALGIVRNGKSLCDTVATLLPISVCETAAADPALVALLMAIAALRREESRGSHFRSDF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGRDAAALPSRLTLCTAFENAVTLRWQASERSR
CCCCHHHCCHHHHHHEECCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968