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The map label for this gene is paaF [C]

Identifier: 13474694

GI number: 13474694

Start: 4530890

End: 4531642

Strand: Direct

Name: paaF [C]

Synonym: mlr5639

Alternate gene names: 13474694

Gene position: 4530890-4531642 (Clockwise)

Preceding gene: 13474693

Following gene: 13474696

Centisome position: 64.4

GC content: 65.6

Gene sequence:

>753_bases
ATGGCCATGAACCCGCACCCGCAACCGGAACTGCGCAACCACACGCTGATCGTCACCGTGTCGTCGAATGACGGCCGGCC
GGTGCTCGACAGAAGGGCCTATGAAAGCCTTGCCAGGACATTCCACGAAGCCGCCGACAATGACGAGGTGCGTGTCGTCG
TGCTGCGCGGCCTGGCAGGCTGCTTCTGCCTTGGCGGCGACTTTTCCGAATTCCTCGACGCCACCAAGCATCAGAAGCTG
ATCGCCGCCGTCACCGATATGTTCCGCACGCTGGCGACGTTCCCCAAGCCCATCCTGGCCTGCGTCGACGGCGATGCCGT
CGGCGTCGGCTGCACCATCTTGTTCCACTGCGACATGGTGATCGCCTCGAATGAAAGCACGTTCCGGGTGCCGTTCGTCG
ATTTCGGCCTTGTGCCGGATGCGGCGACCAGCATCCTGGCGCCGCAGAAGCTCGGTTATGCCGGCGCCTTCCGCTTCTTC
TGTCTCGGCGACACGCTCCACGCCGAGGACGCGAGGGCACTCGGCCTCGTCGCCGAGATCGTGCATGACGGCGTAGAGGA
GGCGACACTCGGCCGCGCCAGGCAGCTCGCCAAGAAGCCGGTTGCCGCCCTGCTGCAGACCCGCGGCCTGCTCAAGGGCA
ACACCGGTGCGCTGTGCGACCGCATCGACCAGGAGATATCGCTGTTCCAGCAGGCATTGCAGGACGACACCACGCTGCGG
CGCCTGCAGCGGATAGCCCGGCTCGCGGCCTAA

Upstream 100 bases:

>100_bases
CGCTGGACGTGGTGCGTTCGCGCCGCAATCACCATGCTCCCGTCCTGGCGCGGGGATATCCCGACATGTTCGGCCAAAGG
AGGGCGTCATGAGGTTGGAG

Downstream 100 bases:

>100_bases
GCAACCGCGCCGCGCTGCCGATGGCCGCGCGGCGCGAGAAGGCCTAGCCTTCCTTGCCGAGGAATGACGGCCCTTCGCCG
ATGATCTTCTTGTCTTCCTT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MAMNPHPQPELRNHTLIVTVSSNDGRPVLDRRAYESLARTFHEAADNDEVRVVVLRGLAGCFCLGGDFSEFLDATKHQKL
IAAVTDMFRTLATFPKPILACVDGDAVGVGCTILFHCDMVIASNESTFRVPFVDFGLVPDAATSILAPQKLGYAGAFRFF
CLGDTLHAEDARALGLVAEIVHDGVEEATLGRARQLAKKPVAALLQTRGLLKGNTGALCDRIDQEISLFQQALQDDTTLR
RLQRIARLAA

Sequences:

>Translated_250_residues
MAMNPHPQPELRNHTLIVTVSSNDGRPVLDRRAYESLARTFHEAADNDEVRVVVLRGLAGCFCLGGDFSEFLDATKHQKL
IAAVTDMFRTLATFPKPILACVDGDAVGVGCTILFHCDMVIASNESTFRVPFVDFGLVPDAATSILAPQKLGYAGAFRFF
CLGDTLHAEDARALGLVAEIVHDGVEEATLGRARQLAKKPVAALLQTRGLLKGNTGALCDRIDQEISLFQQALQDDTTLR
RLQRIARLAA
>Mature_249_residues
AMNPHPQPELRNHTLIVTVSSNDGRPVLDRRAYESLARTFHEAADNDEVRVVVLRGLAGCFCLGGDFSEFLDATKHQKLI
AAVTDMFRTLATFPKPILACVDGDAVGVGCTILFHCDMVIASNESTFRVPFVDFGLVPDAATSILAPQKLGYAGAFRFFC
LGDTLHAEDARALGLVAEIVHDGVEEATLGRARQLAKKPVAALLQTRGLLKGNTGALCDRIDQEISLFQQALQDDTTLRR
LQRIARLAA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI221136756, Length=245, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI221136753, Length=245, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI221307494, Length=245, Percent_Identity=28.1632653061224, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4757966, Length=207, Percent_Identity=27.0531400966184, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538581, Length=207, Percent_Identity=27.0531400966184, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757968, Length=207, Percent_Identity=27.0531400966184, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI49355787, Length=207, Percent_Identity=27.0531400966184, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI25453481, Length=207, Percent_Identity=27.0531400966184, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI51538573, Length=207, Percent_Identity=27.0531400966184, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI116235487, Length=200, Percent_Identity=26.5, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI31542718, Length=196, Percent_Identity=25.5102040816327, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI194097323, Length=152, Percent_Identity=29.6052631578947, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=223, Percent_Identity=26.9058295964126, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787660, Length=186, Percent_Identity=31.7204301075269, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=35.195530726257, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=159, Percent_Identity=27.0440251572327, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=217, Percent_Identity=26.2672811059908, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=221, Percent_Identity=25.3393665158371, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=178, Percent_Identity=26.9662921348315, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324754, Length=134, Percent_Identity=30.5970149253731, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24654903, Length=209, Percent_Identity=29.1866028708134, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20129971, Length=165, Percent_Identity=26.0606060606061, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653477, Length=165, Percent_Identity=26.0606060606061, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667722, Length=227, Percent_Identity=26.8722466960352, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27160; Mature: 27028

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMNPHPQPELRNHTLIVTVSSNDGRPVLDRRAYESLARTFHEAADNDEVRVVVLRGLAG
CCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
CFCLGGDFSEFLDATKHQKLIAAVTDMFRTLATFPKPILACVDGDAVGVGCTILFHCDMV
HEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCHHHEEEEEEECEE
IASNESTFRVPFVDFGLVPDAATSILAPQKLGYAGAFRFFCLGDTLHAEDARALGLVAEI
EECCCCEEEECCCCCCCCCHHHHHHHCHHHCCHHHHHHEEEECCCCCCCHHHHHHHHHHH
VHDGVEEATLGRARQLAKKPVAALLQTRGLLKGNTGALCDRIDQEISLFQQALQDDTTLR
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
RLQRIARLAA
HHHHHHHHCC
>Mature Secondary Structure 
AMNPHPQPELRNHTLIVTVSSNDGRPVLDRRAYESLARTFHEAADNDEVRVVVLRGLAG
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
CFCLGGDFSEFLDATKHQKLIAAVTDMFRTLATFPKPILACVDGDAVGVGCTILFHCDMV
HEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCHHHEEEEEEECEE
IASNESTFRVPFVDFGLVPDAATSILAPQKLGYAGAFRFFCLGDTLHAEDARALGLVAEI
EECCCCEEEECCCCCCCCCHHHHHHHCHHHCCHHHHHHEEEECCCCCCCHHHHHHHHHHH
VHDGVEEATLGRARQLAKKPVAALLQTRGLLKGNTGALCDRIDQEISLFQQALQDDTTLR
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
RLQRIARLAA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA