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The map label for this gene is artJ [H]

Identifier: 13474691

GI number: 13474691

Start: 4528301

End: 4529062

Strand: Reverse

Name: artJ [H]

Synonym: mll5636

Alternate gene names: 13474691

Gene position: 4529062-4528301 (Counterclockwise)

Preceding gene: 13474695

Following gene: 13474689

Centisome position: 64.37

GC content: 61.55

Gene sequence:

>762_bases
GTGCCGGTATTATGGGACGCCAAGGAACGGTTGCCGAAGCCAGACCTTTCAGCGCTGCCCCGGCTGCGCTTCCTGACGAC
CACGGATTTTCCGCCCTTCAATTTCCTCGATGGCACGGGAAAGCTGTCCGGTTTCCACATCGACCTGGCGCGCGCCATCT
GTGCCGAACTCGGCATTGCCGACAAATGCCAGATCCAGGCATTGCCCTGGGGCGAGCTTGAAGGGGCACTCGAGAAAGGC
GAAGGCGAAGCGATCATCGCCGGCATCGCCGCGACGCCGCAATCGCGCCAGACATACGCCTTCTCGCGCTCGTATCTGCA
GTTTCCGGCGCGCTTCATCACGCCGAAGAACAAGGCGTTTGCCGAACCGATCTTCGACAAGCTGCGCAGCAAGCGCGTCG
GCGTTCTCGCCGGCTCGGCGCATGAGCGCATGCTGCGCGACTATTTCAACACGGTCCAAATCGTCTCCTTCGACGGACCG
GAGACCCTCTACAGCGATCTCAAGGCGGGCAAGATCGATGCCGCCTTCGGTGACGGCATGCGTTTCGCCTTCTGGCTGGG
CGGCTCAGATGCAGCCGGGTGCTGCCGTTTTGCCGGCGGTCCTTATCTGGCGCCCGAATATCTGGGCACGGGCATGGCTA
TCGCCACAAGGGCGGACAATCCGGCGCTGGCCGCGGCACTAGACTATGCGCTGCAGGAGATTTCGATGAAGGGCACCTTC
GCCGAATTCTACCTGCGCTATTTCCCGGTAAGTTTTTTCTGA

Upstream 100 bases:

>100_bases
GAGTTCTATCGTGGCGTTCCAATGGAGGTTCCTGGTCCTGGCGTCGCTGCTGATCGCGGCGCTGCCATCGGCTCCAGGGG
CGCGCGCGGCCGAACCGCAG

Downstream 100 bases:

>100_bases
TCCGGTCCCGGTCAGCTTTTTCTGATTCCAGAGCACGGTCTAGGGTTTGTTGAGATTCAGGTCAGGCCGAGCCGACAATG
GTGGGTTCTGAGAACCGTAC

Product: ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPVLWDAKERLPKPDLSALPRLRFLTTTDFPPFNFLDGTGKLSGFHIDLARAICAELGIADKCQIQALPWGELEGALEKG
EGEAIIAGIAATPQSRQTYAFSRSYLQFPARFITPKNKAFAEPIFDKLRSKRVGVLAGSAHERMLRDYFNTVQIVSFDGP
ETLYSDLKAGKIDAAFGDGMRFAFWLGGSDAAGCCRFAGGPYLAPEYLGTGMAIATRADNPALAAALDYALQEISMKGTF
AEFYLRYFPVSFF

Sequences:

>Translated_253_residues
MPVLWDAKERLPKPDLSALPRLRFLTTTDFPPFNFLDGTGKLSGFHIDLARAICAELGIADKCQIQALPWGELEGALEKG
EGEAIIAGIAATPQSRQTYAFSRSYLQFPARFITPKNKAFAEPIFDKLRSKRVGVLAGSAHERMLRDYFNTVQIVSFDGP
ETLYSDLKAGKIDAAFGDGMRFAFWLGGSDAAGCCRFAGGPYLAPEYLGTGMAIATRADNPALAAALDYALQEISMKGTF
AEFYLRYFPVSFF
>Mature_252_residues
PVLWDAKERLPKPDLSALPRLRFLTTTDFPPFNFLDGTGKLSGFHIDLARAICAELGIADKCQIQALPWGELEGALEKGE
GEAIIAGIAATPQSRQTYAFSRSYLQFPARFITPKNKAFAEPIFDKLRSKRVGVLAGSAHERMLRDYFNTVQIVSFDGPE
TLYSDLKAGKIDAAFGDGMRFAFWLGGSDAAGCCRFAGGPYLAPEYLGTGMAIATRADNPALAAALDYALQEISMKGTFA
EFYLRYFPVSFF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=235, Percent_Identity=31.4893617021277, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=29.3103448275862, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788649, Length=240, Percent_Identity=30.8333333333333, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=30.0847457627119, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788228, Length=219, Percent_Identity=25.1141552511416, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27738; Mature: 27607

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLWDAKERLPKPDLSALPRLRFLTTTDFPPFNFLDGTGKLSGFHIDLARAICAELGIA
CCCEECHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCC
DKCQIQALPWGELEGALEKGEGEAIIAGIAATPQSRQTYAFSRSYLQFPARFITPKNKAF
CCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHCCCCCHHH
AEPIFDKLRSKRVGVLAGSAHERMLRDYFNTVQIVSFDGPETLYSDLKAGKIDAAFGDGM
HHHHHHHHHHCCCEEEECCHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCCEEEECCCCC
RFAFWLGGSDAAGCCRFAGGPYLAPEYLGTGMAIATRADNPALAAALDYALQEISMKGTF
EEEEEECCCCCCCHHHCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHH
AEFYLRYFPVSFF
HHHHHHHHCCCCC
>Mature Secondary Structure 
PVLWDAKERLPKPDLSALPRLRFLTTTDFPPFNFLDGTGKLSGFHIDLARAICAELGIA
CCEECHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCC
DKCQIQALPWGELEGALEKGEGEAIIAGIAATPQSRQTYAFSRSYLQFPARFITPKNKAF
CCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHCCCCCHHH
AEPIFDKLRSKRVGVLAGSAHERMLRDYFNTVQIVSFDGPETLYSDLKAGKIDAAFGDGM
HHHHHHHHHHCCCEEEECCHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCCEEEECCCCC
RFAFWLGGSDAAGCCRFAGGPYLAPEYLGTGMAIATRADNPALAAALDYALQEISMKGTF
EEEEEECCCCCCCHHHCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHH
AEFYLRYFPVSFF
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]