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The map label for this gene is fadA [H]

Identifier: 13474684

GI number: 13474684

Start: 4520271

End: 4521479

Strand: Direct

Name: fadA [H]

Synonym: mlr5628

Alternate gene names: 13474684

Gene position: 4520271-4521479 (Clockwise)

Preceding gene: 13474683

Following gene: 13474685

Centisome position: 64.24

GC content: 65.18

Gene sequence:

>1209_bases
ATGGCCGAAGCTTATGTCTATGACGCCGTGCGCACGCCGCGCGGCAAGGGCAAGAAGGACGGATCGCTGCACGAAGTGCC
GGCGGTGCGGCTCGCCGCCAAGACGCTGGAAGCGCTGCGCGACCGCAATGGGCTGGACACAGGTACCGTCGACGACATCA
TCTTCGGCTGCGTCGATCCGGTCGGCGAGGCAGGTTCGGTCATTCCGCGCGCCGCCGCTTTCGAGGCAGGCTATGACACC
AAGGCGCCCGGCATGCAGATCTCGCGCTTCTGCGCCTCGGGTCTCGACGCCATCAATTTCGGCGCCGCCAAGATCGCGCA
GGGCGCCGACGAGATCGTCATTGCCGGTGGCGTCGAATCCATGTCGCGCGTCGGCATGGGCGCCTCCGGCGGCGCCTGGT
TCATGGATCCTTCGGTTGGCCTGCCCGGCTGGTTCGTGCCGCAAGGCATCTCGGCCGACCTGATCGCCACCAAATACGGT
TTCTCCCGTGATGACGTCGACGCTTATGCGGTCGAGAGCCAGAAGCGCGCCGCCAAATCCTGGGCCGACGGCCGCTTCAA
GAATTCGGTCATTCCGATCAAGGACCAGAACGGCCTGACCATTCTGGACCATGACGAGCACATGCGGCCTTCGACCGACA
TGCAGTCTCTGGCTTCGCTCAACCCGTCCTTCGTCATGCCCGGCGAAATGGGCGGCTTCGATGCGGTCGCGGTGCAGAAG
CATCCGGAGGTGGAAGAGGTCAACCACGTCCACCACGCCGGCAACTCGTCCGGCATCGTCGACGGCGCGGCGGCCGTGCT
GCTCGGCTCGAAGAAGGCGGGGAAAGCCATGGGGCTGAAGCCCCGCGCGCGCATCCGCACCTTCGCCAATATCGGCTCCG
AGCCGGTGCTGATGCTGACTGGCCCGGTCGACGTCACCGAGAAGCTGTTGAAGCGCGCCAAGATGAAGCTTTCGGACATC
GACCTGTTCGAGCTCAACGAGGCCTTCGCCTCGGTGGTGCTGCGCTACATGCAGGCCTTCGACATCCCGCACGACAAGAT
CAACGTCAATGGCGGCGCCATCGCCATGGGCCATCCGCTCGGCGCCACAGGCGCGATGATTTTCGGCACGGTGCTGGACG
AACTCGAGCGCCGCGATCTGAACACCGCGCTGGTCACGCTGTGCATCGGCGCCGGCATGGGCACCGCAACGATCATCGAA
CGCGTCTGA

Upstream 100 bases:

>100_bases
CTCGCGTCCAGCGCATCTACGGCGGCACCAACGAGATCATGAAATTGCTGATCGGCCGCTCGCTCTGAAAGCGCCGTCGG
CAAACCCAAGGAGCACGAAA

Downstream 100 bases:

>100_bases
CGGGGAGAGAACAATGAGCTACATCAATTTCACCCTCGACACCGACGCCGACGGCATTGCGCTCGTCACATGGAACATGC
CGGATCGCTCGATGAACGTG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAEAYVYDAVRTPRGKGKKDGSLHEVPAVRLAAKTLEALRDRNGLDTGTVDDIIFGCVDPVGEAGSVIPRAAAFEAGYDT
KAPGMQISRFCASGLDAINFGAAKIAQGADEIVIAGGVESMSRVGMGASGGAWFMDPSVGLPGWFVPQGISADLIATKYG
FSRDDVDAYAVESQKRAAKSWADGRFKNSVIPIKDQNGLTILDHDEHMRPSTDMQSLASLNPSFVMPGEMGGFDAVAVQK
HPEVEEVNHVHHAGNSSGIVDGAAAVLLGSKKAGKAMGLKPRARIRTFANIGSEPVLMLTGPVDVTEKLLKRAKMKLSDI
DLFELNEAFASVVLRYMQAFDIPHDKINVNGGAIAMGHPLGATGAMIFGTVLDELERRDLNTALVTLCIGAGMGTATIIE
RV

Sequences:

>Translated_402_residues
MAEAYVYDAVRTPRGKGKKDGSLHEVPAVRLAAKTLEALRDRNGLDTGTVDDIIFGCVDPVGEAGSVIPRAAAFEAGYDT
KAPGMQISRFCASGLDAINFGAAKIAQGADEIVIAGGVESMSRVGMGASGGAWFMDPSVGLPGWFVPQGISADLIATKYG
FSRDDVDAYAVESQKRAAKSWADGRFKNSVIPIKDQNGLTILDHDEHMRPSTDMQSLASLNPSFVMPGEMGGFDAVAVQK
HPEVEEVNHVHHAGNSSGIVDGAAAVLLGSKKAGKAMGLKPRARIRTFANIGSEPVLMLTGPVDVTEKLLKRAKMKLSDI
DLFELNEAFASVVLRYMQAFDIPHDKINVNGGAIAMGHPLGATGAMIFGTVLDELERRDLNTALVTLCIGAGMGTATIIE
RV
>Mature_401_residues
AEAYVYDAVRTPRGKGKKDGSLHEVPAVRLAAKTLEALRDRNGLDTGTVDDIIFGCVDPVGEAGSVIPRAAAFEAGYDTK
APGMQISRFCASGLDAINFGAAKIAQGADEIVIAGGVESMSRVGMGASGGAWFMDPSVGLPGWFVPQGISADLIATKYGF
SRDDVDAYAVESQKRAAKSWADGRFKNSVIPIKDQNGLTILDHDEHMRPSTDMQSLASLNPSFVMPGEMGGFDAVAVQKH
PEVEEVNHVHHAGNSSGIVDGAAAVLLGSKKAGKAMGLKPRARIRTFANIGSEPVLMLTGPVDVTEKLLKRAKMKLSDID
LFELNEAFASVVLRYMQAFDIPHDKINVNGGAIAMGHPLGATGAMIFGTVLDELERRDLNTALVTLCIGAGMGTATIIER
V

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=409, Percent_Identity=33.7408312958435, Blast_Score=201, Evalue=7e-52,
Organism=Homo sapiens, GI167614485, Length=415, Percent_Identity=33.4939759036145, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=32.9145728643216, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI4557237, Length=416, Percent_Identity=31.4903846153846, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=30.1123595505618, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=47.1910112359551, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787663, Length=429, Percent_Identity=39.1608391608392, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI48994986, Length=412, Percent_Identity=37.621359223301, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082165, Length=415, Percent_Identity=37.3493975903614, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788554, Length=418, Percent_Identity=33.9712918660287, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1788683, Length=431, Percent_Identity=28.538283062645, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI133906874, Length=412, Percent_Identity=32.7669902912621, Blast_Score=200, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI25147385, Length=412, Percent_Identity=30.0970873786408, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17535921, Length=415, Percent_Identity=29.1566265060241, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=450, Percent_Identity=29.1111111111111, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17535917, Length=142, Percent_Identity=32.3943661971831, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322031, Length=360, Percent_Identity=33.6111111111111, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6325229, Length=413, Percent_Identity=30.9927360774818, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24655093, Length=415, Percent_Identity=35.421686746988, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17648125, Length=413, Percent_Identity=35.3510895883777, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24640423, Length=416, Percent_Identity=31.7307692307692, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17137578, Length=444, Percent_Identity=30.8558558558559, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42467; Mature: 42336

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYVYDAVRTPRGKGKKDGSLHEVPAVRLAAKTLEALRDRNGLDTGTVDDIIFGCVDP
CCCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
VGEAGSVIPRAAAFEAGYDTKAPGMQISRFCASGLDAINFGAAKIAQGADEIVIAGGVES
CCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCEEEECCHHH
MSRVGMGASGGAWFMDPSVGLPGWFVPQGISADLIATKYGFSRDDVDAYAVESQKRAAKS
HHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
WADGRFKNSVIPIKDQNGLTILDHDEHMRPSTDMQSLASLNPSFVMPGEMGGFDAVAVQK
HCCCCCCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCEECCCCCCCCCEEEECC
HPEVEEVNHVHHAGNSSGIVDGAAAVLLGSKKAGKAMGLKPRARIRTFANIGSEPVLMLT
CCCHHHHHHHHHCCCCCCCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
GPVDVTEKLLKRAKMKLSDIDLFELNEAFASVVLRYMQAFDIPHDKINVNGGAIAMGHPL
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCC
GATGAMIFGTVLDELERRDLNTALVTLCIGAGMGTATIIERV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
AEAYVYDAVRTPRGKGKKDGSLHEVPAVRLAAKTLEALRDRNGLDTGTVDDIIFGCVDP
CCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
VGEAGSVIPRAAAFEAGYDTKAPGMQISRFCASGLDAINFGAAKIAQGADEIVIAGGVES
CCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCEEEECCHHH
MSRVGMGASGGAWFMDPSVGLPGWFVPQGISADLIATKYGFSRDDVDAYAVESQKRAAKS
HHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
WADGRFKNSVIPIKDQNGLTILDHDEHMRPSTDMQSLASLNPSFVMPGEMGGFDAVAVQK
HCCCCCCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCEECCCCCCCCCEEEECC
HPEVEEVNHVHHAGNSSGIVDGAAAVLLGSKKAGKAMGLKPRARIRTFANIGSEPVLMLT
CCCHHHHHHHHHCCCCCCCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
GPVDVTEKLLKRAKMKLSDIDLFELNEAFASVVLRYMQAFDIPHDKINVNGGAIAMGHPL
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCC
GATGAMIFGTVLDELERRDLNTALVTLCIGAGMGTATIIERV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]