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The map label for this gene is gspE [C]

Identifier: 13474663

GI number: 13474663

Start: 4492110

End: 4493618

Strand: Direct

Name: gspE [C]

Synonym: mlr5602

Alternate gene names: 13474663

Gene position: 4492110-4493618 (Clockwise)

Preceding gene: 13474662

Following gene: 13474664

Centisome position: 63.84

GC content: 63.15

Gene sequence:

>1509_bases
ATGTTCGGTAAAAGAGGCAACGACGACGGCAACCGGTTCACGCCGGAATTCCGCCAGCCGGCACCGGCGCCGGCTGTGCC
GCCGCCCGCGGGTTCGACGGACACGGCGGTTCTTGCCCGGCCCGCGGCACCGATGCAGCCAAGCGCGGCTGTGGCGCCGC
CGGCTCGCCGCGCCGTCGAGGCGCCGCCCATCGCGCCGGAGGCGAGGCGACCCCAGCGCGAAAAGAGCGAAACCTATTAC
GACACCAAGAGCCAGGTTTTCTCGGCGCTCATCGACACGATCGACCTCTCGCAGCTCGCCAAGCTCGACCCGGAGACCGC
GCGCGAGGAAATCCGCGACATCGTCAACGACATCATCGCGATCAAGAATTTCGCGATGTCGATCGCCGAGCAGGAGGAAT
TGCTCGAGGACATCTGCAACGACGTGCTTGGTTATGGGCCGCTCGAGCCATTGCTGGCCCGCGACGACATCGCCGATATC
ATGGTCAACGGCTCCAAGAACGTCTACATCGAAGTCAACGGCAAGGTCGAGCAGACCGGCATTCGCTTTCGTGACAATCA
GCAGCTTCTCAACATCTGCCAGCGCATTGTCAGTCAGGTTGGCCGCCGTGTCGATGAATCCAGCCCGATCTGCGACGCCC
GCCTTCCTGACGGCTCCCGCGTCAACGTCATCGCTCCGCCGCTCGCCATCGACGGCACCGCACTTACCATCCGCAAATTC
AAGAAGGACAAGCTGACGCTCGATCAGCTGGTCAAGTTCGGTGCTATCTCGCCGCAGGGCGCCGAAGTGCTCAAGATCAT
CGGTCGTGTTCGCTGCAACATCGTCATCTCGGGCGGCACCGGCTCCGGCAAGACGACGCTGCTCAACTGCCTGACCAACT
ACATCGACCGCGACGAGCGCGTCATCACCTGCGAGGACTCGGCCGAACTGCAACTGCAGCAGCCGCATGTCGTGCGTCTG
GAAACCCGTCCGCCCAACCTCGAAGGCGAGGGCGAGGTGACGATGCGCGACCTGGTCAAGAACTGCCTGCGCATGCGGCC
CGAGCGCATCATCGTTGGCGAGGTGCGCGGACCCGAAGTGTTCGATCTTCTGCAGGCGATGAACACCGGCCATGACGGCT
CGATGGGAACGATCCACTCGAACAGCCCGCGCGAATGCCTGAACCGTATCGAATCCATGATCGCCATGGGCGGTTATTCG
CTGCCGCAGAAAACCGTGCGTGAAATCGTCGTCGGTTCCATCGACGTGATCATCCAGGCGGCGCGTCTGCGCGACGGATC
GCGCCGTATCACTCACATCACCGAGGTGATCGGGATGGAAGGCGACGTCATCATCACGCAGGATATCGTCCTCTACAACA
TCAAGGGCGAGGACGCTAACGGCAGGCTGCTGGGCGAGCATGTCTCGACCGGCATCGGCCGCCCGCATTTCTGGGACCGG
GCTCGCTACTACGGCGAGGAGCAGCGCCTCGCCGTTGCGCTAGAGGCAATGGAGAAGCGCGCTGACTGA

Upstream 100 bases:

>100_bases
TGCCCATGTGCTGACCGGGCGAAGCGAAATCCGCATGAAAAAGAAGGCTGGCCTGGGCAGCCTGCTCGGCAAGCTCTCGC
GCAACAAGAAGTAGTCGATC

Downstream 100 bases:

>100_bases
TGCGTTGAGGGTTCTGGTCGATGTTTGGAATTGACGGCACCGTATTGGCGTTTGTCGTACTCGCCGGCTTCAGTGCCGGC
GCGGTCGCCTATGCATTCCT

Product: secretory protein kinase, cpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MFGKRGNDDGNRFTPEFRQPAPAPAVPPPAGSTDTAVLARPAAPMQPSAAVAPPARRAVEAPPIAPEARRPQREKSETYY
DTKSQVFSALIDTIDLSQLAKLDPETAREEIRDIVNDIIAIKNFAMSIAEQEELLEDICNDVLGYGPLEPLLARDDIADI
MVNGSKNVYIEVNGKVEQTGIRFRDNQQLLNICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKF
KKDKLTLDQLVKFGAISPQGAEVLKIIGRVRCNIVISGGTGSGKTTLLNCLTNYIDRDERVITCEDSAELQLQQPHVVRL
ETRPPNLEGEGEVTMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHSNSPRECLNRIESMIAMGGYS
LPQKTVREIVVGSIDVIIQAARLRDGSRRITHITEVIGMEGDVIITQDIVLYNIKGEDANGRLLGEHVSTGIGRPHFWDR
ARYYGEEQRLAVALEAMEKRAD

Sequences:

>Translated_502_residues
MFGKRGNDDGNRFTPEFRQPAPAPAVPPPAGSTDTAVLARPAAPMQPSAAVAPPARRAVEAPPIAPEARRPQREKSETYY
DTKSQVFSALIDTIDLSQLAKLDPETAREEIRDIVNDIIAIKNFAMSIAEQEELLEDICNDVLGYGPLEPLLARDDIADI
MVNGSKNVYIEVNGKVEQTGIRFRDNQQLLNICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKF
KKDKLTLDQLVKFGAISPQGAEVLKIIGRVRCNIVISGGTGSGKTTLLNCLTNYIDRDERVITCEDSAELQLQQPHVVRL
ETRPPNLEGEGEVTMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHSNSPRECLNRIESMIAMGGYS
LPQKTVREIVVGSIDVIIQAARLRDGSRRITHITEVIGMEGDVIITQDIVLYNIKGEDANGRLLGEHVSTGIGRPHFWDR
ARYYGEEQRLAVALEAMEKRAD
>Mature_502_residues
MFGKRGNDDGNRFTPEFRQPAPAPAVPPPAGSTDTAVLARPAAPMQPSAAVAPPARRAVEAPPIAPEARRPQREKSETYY
DTKSQVFSALIDTIDLSQLAKLDPETAREEIRDIVNDIIAIKNFAMSIAEQEELLEDICNDVLGYGPLEPLLARDDIADI
MVNGSKNVYIEVNGKVEQTGIRFRDNQQLLNICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKF
KKDKLTLDQLVKFGAISPQGAEVLKIIGRVRCNIVISGGTGSGKTTLLNCLTNYIDRDERVITCEDSAELQLQQPHVVRL
ETRPPNLEGEGEVTMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHSNSPRECLNRIESMIAMGGYS
LPQKTVREIVVGSIDVIIQAARLRDGSRRITHITEVIGMEGDVIITQDIVLYNIKGEDANGRLLGEHVSTGIGRPHFWDR
ARYYGEEQRLAVALEAMEKRAD

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55321; Mature: 55321

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKRGNDDGNRFTPEFRQPAPAPAVPPPAGSTDTAVLARPAAPMQPSAAVAPPARRAVE
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCC
APPIAPEARRPQREKSETYYDTKSQVFSALIDTIDLSQLAKLDPETAREEIRDIVNDIIA
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IKNFAMSIAEQEELLEDICNDVLGYGPLEPLLARDDIADIMVNGSKNVYIEVNGKVEQTG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHEECCCCEEEEEECCEEEECC
IRFRDNQQLLNICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKF
CEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEECCCEEEHHHH
KKDKLTLDQLVKFGAISPQGAEVLKIIGRVRCNIVISGGTGSGKTTLLNCLTNYIDRDER
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCCCE
VITCEDSAELQLQQPHVVRLETRPPNLEGEGEVTMRDLVKNCLRMRPERIIVGEVRGPEV
EEEECCCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHH
FDLLQAMNTGHDGSMGTIHSNSPRECLNRIESMIAMGGYSLPQKTVREIVVGSIDVIIQA
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHH
ARLRDGSRRITHITEVIGMEGDVIITQDIVLYNIKGEDANGRLLGEHVSTGIGRPHFWDR
HHHCCCCHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCCCEEHHHHHCCCCCCCHHHH
ARYYGEEQRLAVALEAMEKRAD
HHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFGKRGNDDGNRFTPEFRQPAPAPAVPPPAGSTDTAVLARPAAPMQPSAAVAPPARRAVE
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCC
APPIAPEARRPQREKSETYYDTKSQVFSALIDTIDLSQLAKLDPETAREEIRDIVNDIIA
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IKNFAMSIAEQEELLEDICNDVLGYGPLEPLLARDDIADIMVNGSKNVYIEVNGKVEQTG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHEECCCCEEEEEECCEEEECC
IRFRDNQQLLNICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKF
CEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEECCCEEEHHHH
KKDKLTLDQLVKFGAISPQGAEVLKIIGRVRCNIVISGGTGSGKTTLLNCLTNYIDRDER
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCCCE
VITCEDSAELQLQQPHVVRLETRPPNLEGEGEVTMRDLVKNCLRMRPERIIVGEVRGPEV
EEEECCCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHH
FDLLQAMNTGHDGSMGTIHSNSPRECLNRIESMIAMGGYSLPQKTVREIVVGSIDVIIQA
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHH
ARLRDGSRRITHITEVIGMEGDVIITQDIVLYNIKGEDANGRLLGEHVSTGIGRPHFWDR
HHHCCCCHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCCCEEHHHHHCCCCCCCHHHH
ARYYGEEQRLAVALEAMEKRAD
HHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]