BacMap

Definition	Mesorhizobium loti MAFF303099 chromosome, complete genome.
Accession	NC_002678
Length	7,036,071

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The map label for this gene is fadB1 [H]

Identifier: 13474650

GI number: 13474650

Start: 4480246

End: 4481019

Strand: Reverse

Name: fadB1 [H]

Synonym: mll5584

Alternate gene names: 13474650

Gene position: 4481019-4480246 (Counterclockwise)

Preceding gene: 13474651

Following gene: 13474649

Centisome position: 63.69

GC content: 62.79

Gene sequence:

>774_bases
ATGGCCTATGAAACGATCATCACCGAGACACGCGGCAAGGTCGGGCTGGTCACGCTGAACCGGCCAAAGGCGCTCAATGC
GCTGAACCTTCAGCTTCTCGCCGAACTGGTCGCGGCCGTGAAGGACTTCGACGCCGATGCCGGCATCGGCGCGGTGGTCA
TTACCGGTTCGGACAAGGCCTTTGCCGCCGGCGCCGACATCAAGGAGATGCAAGCAATCTCCTTTGCGGACGCCTATTTG
CAGGACCTGTTCGTCGGCTGGGAAGACCTGACGCGGGTGCGGAAACCCGTTATCGCCGCGGTGGCCGGCTATGCGCTCGG
CGGCGGGTGCGAGCTGGCGATGATGTGCGACTTTATCATTGCCGCTGACACGGCCAAATTCGGCCAGCCCGAAATCACGC
TCGGCGTCATTCCCGGCATGGGCGGATCGCAACGCCTGACCAGGTTTGTCGGCAAGTCTAAGGCGATGGACATGTGCCTG
ACCGGACGGATGATGGATGCAGCGGAAGCCGAGCGCTCGGGCCTGGTGTCGCGGGTCGTGCCCGCCGCCGAGCTTGTCGA
GGAGGCGGTCAAGGCGGCGGCCAAGATCGCCGATTTCTCGCTGCCCTCGGTGATGATGGCGAAGGAAGCCGTCAACCGTT
CCTATGAGACGACGCTGACCGAGGGATTGCGGTTCGAACGTCGTCTGTTCCACTCGCTTTTCGCGCTCGAAGACCAGAAG
GAAGGCATGGCGGCCTTCGCCGAAAAGCGGAAGCCGAATTTCGCCAATCGGTAG

Upstream 100 bases:

>100_bases
GGCGGACATATCGAGCGCGTCGTGCAGAGCGGACGCTCGACCTTCTATTGCCGGACGTGTCAGAGCTGAGTTAGACCGAC
GCAAGCGAGGAGAAGCAGCC

Downstream 100 bases:

>100_bases
AGCCTTGCCCGCCCGCGACGGAAACCCTGTGGCGCGGGCCAAAAAGAACGCCAGGCAGCGTTGACGCGCCGGAAAAGCTG
AACTATAAGCCGCACCAACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAYETIITETRGKVGLVTLNRPKALNALNLQLLAELVAAVKDFDADAGIGAVVITGSDKAFAAGADIKEMQAISFADAYL
QDLFVGWEDLTRVRKPVIAAVAGYALGGGCELAMMCDFIIAADTAKFGQPEITLGVIPGMGGSQRLTRFVGKSKAMDMCL
TGRMMDAAEAERSGLVSRVVPAAELVEEAVKAAAKIADFSLPSVMMAKEAVNRSYETTLTEGLRFERRLFHSLFALEDQK
EGMAAFAEKRKPNFANR

Sequences:

>Translated_257_residues
MAYETIITETRGKVGLVTLNRPKALNALNLQLLAELVAAVKDFDADAGIGAVVITGSDKAFAAGADIKEMQAISFADAYL
QDLFVGWEDLTRVRKPVIAAVAGYALGGGCELAMMCDFIIAADTAKFGQPEITLGVIPGMGGSQRLTRFVGKSKAMDMCL
TGRMMDAAEAERSGLVSRVVPAAELVEEAVKAAAKIADFSLPSVMMAKEAVNRSYETTLTEGLRFERRLFHSLFALEDQK
EGMAAFAEKRKPNFANR
>Mature_256_residues
AYETIITETRGKVGLVTLNRPKALNALNLQLLAELVAAVKDFDADAGIGAVVITGSDKAFAAGADIKEMQAISFADAYLQ
DLFVGWEDLTRVRKPVIAAVAGYALGGGCELAMMCDFIIAADTAKFGQPEITLGVIPGMGGSQRLTRFVGKSKAMDMCLT
GRMMDAAEAERSGLVSRVVPAAELVEEAVKAAAKIADFSLPSVMMAKEAVNRSYETTLTEGLRFERRLFHSLFALEDQKE
GMAAFAEKRKPNFANR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=59.3023255813954, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=31.9838056680162, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI68989263, Length=232, Percent_Identity=33.1896551724138, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.9548872180451, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=37.2222222222222, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI37594471, Length=217, Percent_Identity=29.4930875576037, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI37594469, Length=217, Percent_Identity=29.4930875576037, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=30.8943089430894, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=27.3972602739726, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=27.3972602739726, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=27.3972602739726, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=35.5555555555556, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI157694520, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI62530384, Length=200, Percent_Identity=30.5, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI25453481, Length=218, Percent_Identity=26.1467889908257, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538573, Length=218, Percent_Identity=26.1467889908257, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757966, Length=207, Percent_Identity=25.6038647342995, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=207, Percent_Identity=25.6038647342995, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=259, Percent_Identity=23.1660231660232, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI49355787, Length=259, Percent_Identity=23.1660231660232, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=197, Percent_Identity=27.4111675126904, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=47.4308300395257, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.9230769230769, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=33.852140077821, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=32.4427480916031, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=30.3030303030303, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=34.010152284264, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=34.0540540540541, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=57.8947368421053, Blast_Score=289, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=52.0491803278688, Blast_Score=262, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17540714, Length=242, Percent_Identity=33.4710743801653, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=33.9920948616601, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=32.7800829875519, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25144276, Length=213, Percent_Identity=36.6197183098592, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17508953, Length=235, Percent_Identity=34.468085106383, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=213, Percent_Identity=36.6197183098592, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=34.0540540540541, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144160, Length=231, Percent_Identity=32.4675324675325, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144157, Length=231, Percent_Identity=32.4675324675325, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=273, Percent_Identity=29.3040293040293, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=237, Percent_Identity=32.9113924050633, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=29.1828793774319, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=250, Percent_Identity=26.4, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=27.8688524590164, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=59.9221789883268, Blast_Score=308, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=59.9221789883268, Blast_Score=308, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=37.007874015748, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=30.8880308880309, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=34.1232227488152, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=29.296875, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19920382, Length=256, Percent_Identity=32.421875, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654903, Length=253, Percent_Identity=24.901185770751, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571729, Length=242, Percent_Identity=28.5123966942149, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28571730, Length=242, Percent_Identity=28.5123966942149, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=34.7593582887701, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=34.7593582887701, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=34.7593582887701, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921018, Length=201, Percent_Identity=29.3532338308458, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583165, Length=197, Percent_Identity=29.4416243654822, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27580; Mature: 27449

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYETIITETRGKVGLVTLNRPKALNALNLQLLAELVAAVKDFDADAGIGAVVITGSDKA
CCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIKEMQAISFADAYLQDLFVGWEDLTRVRKPVIAAVAGYALGGGCELAMMCDFII
EECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEITLGVIPGMGGSQRLTRFVGKSKAMDMCLTGRMMDAAEAERSGLVSRVV
HHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
PAAELVEEAVKAAAKIADFSLPSVMMAKEAVNRSYETTLTEGLRFERRLFHSLFALEDQK
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
EGMAAFAEKRKPNFANR
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYETIITETRGKVGLVTLNRPKALNALNLQLLAELVAAVKDFDADAGIGAVVITGSDKA
CCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIKEMQAISFADAYLQDLFVGWEDLTRVRKPVIAAVAGYALGGGCELAMMCDFII
EECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEITLGVIPGMGGSQRLTRFVGKSKAMDMCLTGRMMDAAEAERSGLVSRVV
HHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
PAAELVEEAVKAAAKIADFSLPSVMMAKEAVNRSYETTLTEGLRFERRLFHSLFALEDQK
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
EGMAAFAEKRKPNFANR
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]