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The map label for this gene is dnaA [H]

Identifier: 13474648

GI number: 13474648

Start: 4477398

End: 4478942

Strand: Reverse

Name: dnaA [H]

Synonym: mll5581

Alternate gene names: 13474648

Gene position: 4478942-4477398 (Counterclockwise)

Preceding gene: 13474649

Following gene: 13474647

Centisome position: 63.66

GC content: 63.95

Gene sequence:

>1545_bases
ATGCAGAGCGGCATCGAAAGGGAGCTTACGGGCGACCTCCCATTTCCTGGAACTTTGATCGGAGCAAACGAGATGGCGGT
CTCCAGCGACGCGGAACAGAAGTTCGACCGGGTCAAGACCCAGTTGAAGGCGCGTCTGGGAGCTGAAGTCTATTCGAGCT
GGTTCGGCCGCATGAAGGTCGCGGAAGCGTCCAAGGGCATTGTCCGCATCTCGGTCCCCACCGCGTTCCTGCGCTCGTGG
ATCAATGGTCATTATCTCGACCTCATCTCCGAACTGTGGAAGCAGGAGGATGCCGATCTCCTCAAGATCGAGATCGTGGT
GCGCACGGCCACCCGTCAGGGGCGCAGCCATGCCGAACCGGAGTTGGCGCCGGCGCGCAAGATGACACGCCAGACGCAGA
CGGCGCTGGCCGCCGGCACTGTAAGCCCCGGCAGGGTGGAACGGCCTCCCGTGCCGCGCCCGGGTGCGGCGGTCGAGAGC
GAGTTCCGCCACAATGTGCTGGGGTCACCGCTTGATCCGCGCTACACGTTCGGCTCCTTCATCGAGGGGCCGTCGAACCG
GGTGGCCTTCGCCGCCGCCAAGGCCGTGGCGGAATCGCAGTCGAGCGCCGTGCGCTTCAATCCGCTTTTCCTGCACGCGA
CCGTCGGGCTCGGCAAGACCCACCTCCTGCAGGCCATCGCGGCGGAATCGCTGAAGCAGAACCCGAAGTCCCGCGTCGTC
TATCTCACCGCGGAGTATTTCATGTGGCGCTTCGCCACGGCGATCCGCGACAACAACGCGCTGACGCTCAAGGAGCAACT
GCGCGACATCGACCTCCTGATCATCGACGACATGCAGTTCCTGCAGGGCAAGTCGATCCAGCATGAATTCTGCCATTTGA
TCAACATGCTGCTCGACAGCGCCAAGCAGGTCGTTGTCGCCGCCGACCGGCCGCCGTCCGAGCTGGAATCGCTCGAGCCG
CGCGTCCGCTCGCGCCTCAATGGCGGCGTCGCGCTTGAAATGTCGGCGCCTGATTTCGCCATGCGCCTCGGCATGCTCAA
ACTGCGCCTGGCCACGGCCAGGATCGACGACGCATCGCTCGACATTTCCGACGAGATCCTCAATCACGTCGCCCGCACCG
TGACCGGCAGCGGACGCGAACTGGAGGGCGCCTTCAATCAGCTGCTGTTCCGGCAGTCGTTCGAGCCGCAGATCACCATC
GATCGTATCGACGAGATCCTCGGCCACATCTATCGCACCGGCGAGCCGAAGCGGGTCCGTATCGAGGATATCCAGCGCAT
CGTGGCGCGCCACTACAATGTGTCGAAGACCGAATTGCTGTCCAATCGGCGCACGCGCACCATCGTCAAGCCGAGGCAGG
TCGCCATGTACCTGTCGAAGGTGATGACGCCGCGCTCCCTGCCCGAAATCGGACGGCGCTTCGGCGGCCGCGACCACACC
ACGGTTCTGCATGCCGTGCGCAAGATCGAGGACCTTTCCGGCAACGACAACACGCTGGCCCAGGAACTCGAGCTGTTGCG
GCGGCTGATCAACGACCAGGCCTGA

Upstream 100 bases:

>100_bases
GCAAAGGGCATCGTAGCGAAGACAAAGTCGCTGGAAACAAGGATGCAGGAGGCGCATCCCCAGCGAAAAAACAGGTACTA
GAAAGACAAGGAACTCGATC

Downstream 100 bases:

>100_bases
CCGGTCCAGAACAGGCAAGCCGGAACTGCGCTAGAATTCAATGGCTTGCCTGCCCAGCGATGAGCCCGAAATGGGCTCGC
GGTATCGGCCGGCGACGATC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MQSGIERELTGDLPFPGTLIGANEMAVSSDAEQKFDRVKTQLKARLGAEVYSSWFGRMKVAEASKGIVRISVPTAFLRSW
INGHYLDLISELWKQEDADLLKIEIVVRTATRQGRSHAEPELAPARKMTRQTQTALAAGTVSPGRVERPPVPRPGAAVES
EFRHNVLGSPLDPRYTFGSFIEGPSNRVAFAAAKAVAESQSSAVRFNPLFLHATVGLGKTHLLQAIAAESLKQNPKSRVV
YLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLIIDDMQFLQGKSIQHEFCHLINMLLDSAKQVVVAADRPPSELESLEP
RVRSRLNGGVALEMSAPDFAMRLGMLKLRLATARIDDASLDISDEILNHVARTVTGSGRELEGAFNQLLFRQSFEPQITI
DRIDEILGHIYRTGEPKRVRIEDIQRIVARHYNVSKTELLSNRRTRTIVKPRQVAMYLSKVMTPRSLPEIGRRFGGRDHT
TVLHAVRKIEDLSGNDNTLAQELELLRRLINDQA

Sequences:

>Translated_514_residues
MQSGIERELTGDLPFPGTLIGANEMAVSSDAEQKFDRVKTQLKARLGAEVYSSWFGRMKVAEASKGIVRISVPTAFLRSW
INGHYLDLISELWKQEDADLLKIEIVVRTATRQGRSHAEPELAPARKMTRQTQTALAAGTVSPGRVERPPVPRPGAAVES
EFRHNVLGSPLDPRYTFGSFIEGPSNRVAFAAAKAVAESQSSAVRFNPLFLHATVGLGKTHLLQAIAAESLKQNPKSRVV
YLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLIIDDMQFLQGKSIQHEFCHLINMLLDSAKQVVVAADRPPSELESLEP
RVRSRLNGGVALEMSAPDFAMRLGMLKLRLATARIDDASLDISDEILNHVARTVTGSGRELEGAFNQLLFRQSFEPQITI
DRIDEILGHIYRTGEPKRVRIEDIQRIVARHYNVSKTELLSNRRTRTIVKPRQVAMYLSKVMTPRSLPEIGRRFGGRDHT
TVLHAVRKIEDLSGNDNTLAQELELLRRLINDQA
>Mature_514_residues
MQSGIERELTGDLPFPGTLIGANEMAVSSDAEQKFDRVKTQLKARLGAEVYSSWFGRMKVAEASKGIVRISVPTAFLRSW
INGHYLDLISELWKQEDADLLKIEIVVRTATRQGRSHAEPELAPARKMTRQTQTALAAGTVSPGRVERPPVPRPGAAVES
EFRHNVLGSPLDPRYTFGSFIEGPSNRVAFAAAKAVAESQSSAVRFNPLFLHATVGLGKTHLLQAIAAESLKQNPKSRVV
YLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLIIDDMQFLQGKSIQHEFCHLINMLLDSAKQVVVAADRPPSELESLEP
RVRSRLNGGVALEMSAPDFAMRLGMLKLRLATARIDDASLDISDEILNHVARTVTGSGRELEGAFNQLLFRQSFEPQITI
DRIDEILGHIYRTGEPKRVRIEDIQRIVARHYNVSKTELLSNRRTRTIVKPRQVAMYLSKVMTPRSLPEIGRRFGGRDHT
TVLHAVRKIEDLSGNDNTLAQELELLRRLINDQA

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=476, Percent_Identity=34.6638655462185, Blast_Score=264, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 57552; Mature: 57552

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS01008 DNAA ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSGIERELTGDLPFPGTLIGANEMAVSSDAEQKFDRVKTQLKARLGAEVYSSWFGRMKV
CCCCCCCCCCCCCCCCCEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEH
AEASKGIVRISVPTAFLRSWINGHYLDLISELWKQEDADLLKIEIVVRTATRQGRSHAEP
HHCCCCEEEEECCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEEHHHCCCCCCCC
ELAPARKMTRQTQTALAAGTVSPGRVERPPVPRPGAAVESEFRHNVLGSPLDPRYTFGSF
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
IEGPSNRVAFAAAKAVAESQSSAVRFNPLFLHATVGLGKTHLLQAIAAESLKQNPKSRVV
CCCCCCCHHHHHHHHHHHCCCCCEEECEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
YLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLIIDDMQFLQGKSIQHEFCHLINMLLDS
EEEHHHHHHHHHHHHHCCCCEEHHHHHCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHC
AKQVVVAADRPPSELESLEPRVRSRLNGGVALEMSAPDFAMRLGMLKLRLATARIDDASL
CCEEEEECCCCHHHHHHHCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
DISDEILNHVARTVTGSGRELEGAFNQLLFRQSFEPQITIDRIDEILGHIYRTGEPKRVR
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCEEE
IEDIQRIVARHYNVSKTELLSNRRTRTIVKPRQVAMYLSKVMTPRSLPEIGRRFGGRDHT
HHHHHHHHHHHCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHH
TVLHAVRKIEDLSGNDNTLAQELELLRRLINDQA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSGIERELTGDLPFPGTLIGANEMAVSSDAEQKFDRVKTQLKARLGAEVYSSWFGRMKV
CCCCCCCCCCCCCCCCCEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEH
AEASKGIVRISVPTAFLRSWINGHYLDLISELWKQEDADLLKIEIVVRTATRQGRSHAEP
HHCCCCEEEEECCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEEHHHCCCCCCCC
ELAPARKMTRQTQTALAAGTVSPGRVERPPVPRPGAAVESEFRHNVLGSPLDPRYTFGSF
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
IEGPSNRVAFAAAKAVAESQSSAVRFNPLFLHATVGLGKTHLLQAIAAESLKQNPKSRVV
CCCCCCCHHHHHHHHHHHCCCCCEEECEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
YLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLIIDDMQFLQGKSIQHEFCHLINMLLDS
EEEHHHHHHHHHHHHHCCCCEEHHHHHCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHC
AKQVVVAADRPPSELESLEPRVRSRLNGGVALEMSAPDFAMRLGMLKLRLATARIDDASL
CCEEEEECCCCHHHHHHHCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
DISDEILNHVARTVTGSGRELEGAFNQLLFRQSFEPQITIDRIDEILGHIYRTGEPKRVR
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCEEE
IEDIQRIVARHYNVSKTELLSNRRTRTIVKPRQVAMYLSKVMTPRSLPEIGRRFGGRDHT
HHHHHHHHHHHCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHH
TVLHAVRKIEDLSGNDNTLAQELELLRRLINDQA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]