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The map label for this gene is prfC [H]

Identifier: 13474465

GI number: 13474465

Start: 4275623

End: 4277215

Strand: Direct

Name: prfC [H]

Synonym: mlr5356

Alternate gene names: 13474465

Gene position: 4275623-4277215 (Clockwise)

Preceding gene: 13474464

Following gene: 13474469

Centisome position: 60.77

GC content: 64.16

Gene sequence:

>1593_bases
ATGCCCGAAACAATACCAGAGGAAGTGGCGCGCCGCCGCACCTTCGCGATCATCGCGCACCCCGACGCCGGCAAGACGAC
GCTCACCGAAAAGCTGCTGCTGTTCGGCGGCGCCATCCAGCTTGCCGGCGAGGTCAAGGCCAAGAAGGACAAGATCCAGA
CCCGGTCCGACTGGATGAAGATCGAGCGCGAGCGCGGCATTTCGGTCGTCACTTCGGTGATGACCTTCGAGTATGAAGAC
AATGTCTTCAACCTGCTCGACACGCCCGGCCACGAGGATTTCGCCGACGATACCTACCGCACGCTGTCGGCCGTCGACTC
GGCCGTCATGGTCATCGACGCCGCCAAGGGCATCGAGCCGCGCACGCTGAAACTGTTCGAAGTCTGCCGGTTGCGCGACA
TCCCGATCATCACTTTCGTCAACAAGATGGACCGCGAAAGCCGCGATCCGTTCGAGATTCTGGACGAGATCGAGCAGAAG
CTGGCGCTGGACACCGCACCCATCACTTGGCCGATCGGCCGCGGCAGGACGTTTTCCGGCACCTATCACCTGGCGCTGAA
CGCGGTGCGCAAGGGCGACGACGAGAAGGAACGCACGCCGGTCAACGGTCCCGATTCCAACCGCGTCGCCGGGCTGCTGC
CGGAGAACGAGCGCGAGGCCTTCATCGAGGAACTGGAGCTCGCGCGCGAAGCCTGCCGGCCGCTTGATATTGATGCCTTC
CGCGAGGGCCATTTGACGCCGGTCTATTTCGGCTCGGCGCTGCGCAACTATGGCGTGCGCGACCTGATCGAGGCGCTCGG
CGCCTTCGGCCCGCCGCCGCGCGCGCAGGAGGCCGACGCCCGCAAGGTCGAGGCGACCGAGGACAAGATGACCTCCTTCG
TCTTCAAGATCCAGGCCAATATGGACCCCAATCACCGCGACCGTATCGCTTTCGTGCGCGTCTGCTCGGGCAAGCTCGAG
CGTGGCATGAAGGCCAAGCTGGTGCGAACCGGCAAGCCGATGAGCCTGTCGGCGCCGCAGTTCTTCTTCGCCCGCACCCG
CGTCACGGCGGATGAAGCCTTTGCCGGCGACGTCGTCGGCATTCCCAACCACGGCACGCTGCGCATCGGTGACACGCTGA
CCGAGGGCGAGGAAATTCTTTTCAGGGGCGTGCCGAACTTCGCGCCGGAAATCCTGCGCCGTGTGCGCCTCGGCGACGCC
ATGAAGGCGAAGAAACTCAAGGAAGCGCTGCACCAGATGGCCGAGGAAGGCGTCGTGCAGCTATTCTCGCCCGAGGATGG
CTCACCGGCCATCGTCGGCGTCGTCGGCGCCCTGCAGCTCGACGTGTTGAAAGAGCGGCTGAACTTCGAATACACACTGC
CGGTCGAATTCGAAATGTCGCGCTTTTCCGTCTGCCGCTGGATCTCGGCCGACGACAAGGCCGAGACGCTTCGCTTCATC
GAGGCGCATCGCGGCGACATCGCCAGGGACCTCGACAACGACCCGGTGTTCCTGGCCCAGCACGCCTTCTCGCTGAACTA
CGAAGCCGAGCGCTGGAAAGCGATCCGCTTCGCCACCATCAAGGACTACCAGGTCCGCGACAAGGCGGCGTGA

Upstream 100 bases:

>100_bases
GGCAAGCCATGGCATCCGGCGGCGTCCGCGCCGCCGGACCGGTCGACAGCCCGGCACTACGCTGCTAGAAGCCCGGCCTG
TCACACGGAAATCCCAGCGA

Downstream 100 bases:

>100_bases
GCTGTGGTTCTTCCTTCTCCCACAAGGGAGAAGGGAAAACAGTGCAGTGCTCACCCTTTCGCCGCCGCTTCGAGCTTGGC
GATGTCGATCTTGCCCATCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MPETIPEEVARRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDKIQTRSDWMKIERERGISVVTSVMTFEYED
NVFNLLDTPGHEDFADDTYRTLSAVDSAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEIEQK
LALDTAPITWPIGRGRTFSGTYHLALNAVRKGDDEKERTPVNGPDSNRVAGLLPENEREAFIEELELAREACRPLDIDAF
REGHLTPVYFGSALRNYGVRDLIEALGAFGPPPRAQEADARKVEATEDKMTSFVFKIQANMDPNHRDRIAFVRVCSGKLE
RGMKAKLVRTGKPMSLSAPQFFFARTRVTADEAFAGDVVGIPNHGTLRIGDTLTEGEEILFRGVPNFAPEILRRVRLGDA
MKAKKLKEALHQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLNFEYTLPVEFEMSRFSVCRWISADDKAETLRFI
EAHRGDIARDLDNDPVFLAQHAFSLNYEAERWKAIRFATIKDYQVRDKAA

Sequences:

>Translated_530_residues
MPETIPEEVARRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDKIQTRSDWMKIERERGISVVTSVMTFEYED
NVFNLLDTPGHEDFADDTYRTLSAVDSAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEIEQK
LALDTAPITWPIGRGRTFSGTYHLALNAVRKGDDEKERTPVNGPDSNRVAGLLPENEREAFIEELELAREACRPLDIDAF
REGHLTPVYFGSALRNYGVRDLIEALGAFGPPPRAQEADARKVEATEDKMTSFVFKIQANMDPNHRDRIAFVRVCSGKLE
RGMKAKLVRTGKPMSLSAPQFFFARTRVTADEAFAGDVVGIPNHGTLRIGDTLTEGEEILFRGVPNFAPEILRRVRLGDA
MKAKKLKEALHQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLNFEYTLPVEFEMSRFSVCRWISADDKAETLRFI
EAHRGDIARDLDNDPVFLAQHAFSLNYEAERWKAIRFATIKDYQVRDKAA
>Mature_529_residues
PETIPEEVARRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDKIQTRSDWMKIERERGISVVTSVMTFEYEDN
VFNLLDTPGHEDFADDTYRTLSAVDSAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEIEQKL
ALDTAPITWPIGRGRTFSGTYHLALNAVRKGDDEKERTPVNGPDSNRVAGLLPENEREAFIEELELAREACRPLDIDAFR
EGHLTPVYFGSALRNYGVRDLIEALGAFGPPPRAQEADARKVEATEDKMTSFVFKIQANMDPNHRDRIAFVRVCSGKLER
GMKAKLVRTGKPMSLSAPQFFFARTRVTADEAFAGDVVGIPNHGTLRIGDTLTEGEEILFRGVPNFAPEILRRVRLGDAM
KAKKLKEALHQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLNFEYTLPVEFEMSRFSVCRWISADDKAETLRFIE
AHRGDIARDLDNDPVFLAQHAFSLNYEAERWKAIRFATIKDYQVRDKAA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=490, Percent_Identity=26.3265306122449, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI19923640, Length=506, Percent_Identity=26.4822134387352, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25306283, Length=399, Percent_Identity=28.8220551378446, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI25306287, Length=296, Percent_Identity=31.4189189189189, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI157426893, Length=151, Percent_Identity=35.7615894039735, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=32.3943661971831, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI310132016, Length=123, Percent_Identity=32.520325203252, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=123, Percent_Identity=32.520325203252, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=123, Percent_Identity=32.520325203252, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4503483, Length=159, Percent_Identity=29.559748427673, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=48.8505747126437, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=28.4848484848485, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=35.031847133758, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=33.5570469798658, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=489, Percent_Identity=26.5848670756646, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=211, Percent_Identity=35.0710900473934, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=144, Percent_Identity=34.7222222222222, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=493, Percent_Identity=29.2089249492901, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=538, Percent_Identity=24.1635687732342, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=167, Percent_Identity=31.1377245508982, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=29.746835443038, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=29.746835443038, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=481, Percent_Identity=26.4033264033264, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI221458488, Length=510, Percent_Identity=27.4509803921569, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=32.5, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.0261437908497, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.0261437908497, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.0261437908497, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=31.25, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59614; Mature: 59482

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETIPEEVARRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDKIQTRSDWMK
CCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYEDNVFNLLDTPGHEDFADDTYRTLSAVDSAVMVIDAAKGIEP
HHHHCCHHHHHHHHEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCC
RTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEIEQKLALDTAPITWPIGRGRTFSG
CHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEECC
TYHLALNAVRKGDDEKERTPVNGPDSNRVAGLLPENEREAFIEELELAREACRPLDIDAF
EEEEEEHHHHCCCCHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHH
REGHLTPVYFGSALRNYGVRDLIEALGAFGPPPRAQEADARKVEATEDKMTSFVFKIQAN
CCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEECC
MDPNHRDRIAFVRVCSGKLERGMKAKLVRTGKPMSLSAPQFFFARTRVTADEAFAGDVVG
CCCCCCHHEEHEEHHHHHHHCCCCEEHEECCCCCCCCCCHHHHHHHCCCCCHHCCCCEEE
IPNHGTLRIGDTLTEGEEILFRGVPNFAPEILRRVRLGDAMKAKKLKEALHQMAEEGVVQ
CCCCCEEEECCCCCCCCHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEE
LFSPEDGSPAIVGVVGALQLDVLKERLNFEYTLPVEFEMSRFSVCRWISADDKAETLRFI
EECCCCCCCEEEHHHHHHHHHHHHHHHCCEEEECEEECCCHHHHHEECCCCCHHHHHHHH
EAHRGDIARDLDNDPVFLAQHAFSLNYEAERWKAIRFATIKDYQVRDKAA
HHHCCCHHHCCCCCCEEEEEHHEECCCCHHHHEEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
PETIPEEVARRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDKIQTRSDWMK
CCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYEDNVFNLLDTPGHEDFADDTYRTLSAVDSAVMVIDAAKGIEP
HHHHCCHHHHHHHHEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCC
RTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEIEQKLALDTAPITWPIGRGRTFSG
CHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEECC
TYHLALNAVRKGDDEKERTPVNGPDSNRVAGLLPENEREAFIEELELAREACRPLDIDAF
EEEEEEHHHHCCCCHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHH
REGHLTPVYFGSALRNYGVRDLIEALGAFGPPPRAQEADARKVEATEDKMTSFVFKIQAN
CCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEECC
MDPNHRDRIAFVRVCSGKLERGMKAKLVRTGKPMSLSAPQFFFARTRVTADEAFAGDVVG
CCCCCCHHEEHEEHHHHHHHCCCCEEHEECCCCCCCCCCHHHHHHHCCCCCHHCCCCEEE
IPNHGTLRIGDTLTEGEEILFRGVPNFAPEILRRVRLGDAMKAKKLKEALHQMAEEGVVQ
CCCCCEEEECCCCCCCCHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEE
LFSPEDGSPAIVGVVGALQLDVLKERLNFEYTLPVEFEMSRFSVCRWISADDKAETLRFI
EECCCCCCCEEEHHHHHHHHHHHHHHHCCEEEECEEECCCHHHHHEECCCCCHHHHHHHH
EAHRGDIARDLDNDPVFLAQHAFSLNYEAERWKAIRFATIKDYQVRDKAA
HHHCCCHHHCCCCCCEEEEEHHEECCCCHHHHEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA