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The map label for this gene is dhaA

Identifier: 13474464

GI number: 13474464

Start: 4274593

End: 4275522

Strand: Direct

Name: dhaA

Synonym: mlr5354

Alternate gene names: 13474464

Gene position: 4274593-4275522 (Clockwise)

Preceding gene: 13474463

Following gene: 13474465

Centisome position: 60.75

GC content: 64.3

Gene sequence:

>930_bases
ATGAGTTCGAAAGCCAACCCGCCTCAGCCGGTCGCCACCGCGCCGAAACGTTCGCAGATTCCCATACTCGATTCGACCAT
GTCCTATGTCGAGGCGGGAGCGTCCGGCCCGACCGTGCTGTTCCTGCATGGCAACCCGACCTCGTCCCATATCTGGCGCA
ACATCATCCCCCATGTCGCGCCGTTCGGCCGCTGCATCGCGCCTGACCTGATCGGCTATGGCCAGTCCGGCAAGCCCGAT
ATCGACTATCGCTTCTTCGACCATGTCCGCTATCTCGACGCCTTCCTCGACGCCCTGGACATCAGGGATGTGCTGTTGGT
TGCGCAGGATTGGGGCACGGCCCTTGCCTTCCATCTCGCGGCAAGGCGGCCGCAGCGCGTCCTCGGCCTCGCCTTCATGG
AATTCATCCGGCCCTTCGAGCGCTGGGAGGATTTTCACCAGCGCCCGCAAGCTCGCGAAATGTTCAAGGCGCTGCGCACG
CCCGGCGTCGGCGAAAAGCTGGTCCTGGAAGACAATGTCTTCGTTGAGAAAGTGCTGCCGGCCTCGGTGTTGCGCGCGAT
GAGCGACGACGAGATGGACGTCTACCGGGCGCCGTTCCCGACGCCGCAATCGCGCAAGCCGGTGCTGCGCCTGCCCCGGG
AAATGCCCATCGAGGGTCAGCCCGCCGACGTTGCCGCCATCAGCGCGCACGACCATCGCGCGCTGCGGCTGTCCACCTAT
CCGAAACTGCTGTTCGCGGGTGATCCCGGGGCGCTGATCGGGCCGCAGGCCGCGCGCGAATTCGCGGCCGGTTTGAAGAA
TTGCAGTTTCATCAATCTCGGGCCTGGTGCGCACTATCTGCAGGAGGATCATGCCGATGCGATAGGCCGCGCTATCGCCA
GCTGGCTTCCGGAGGTCGTTTTGGCGAACCAGACGGACGAGTTGGCCTGA

Upstream 100 bases:

>100_bases
GGCAGGGCGCCATGCTGCGCATGAAGGTCGAGCGCAGCCCCGAACCGCTCGAGCGGTTCAAGAAAATCATCTTCAACACA
GCGTTTGCAAAGGAGACCCC

Downstream 100 bases:

>100_bases
GGCAAGCCATGGCATCCGGCGGCGTCCGCGCCGCCGGACCGGTCGACAGCCCGGCACTACGCTGCTAGAAGCCCGGCCTG
TCACACGGAAATCCCAGCGA

Product: haloalkane dehalogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSSKANPPQPVATAPKRSQIPILDSTMSYVEAGASGPTVLFLHGNPTSSHIWRNIIPHVAPFGRCIAPDLIGYGQSGKPD
IDYRFFDHVRYLDAFLDALDIRDVLLVAQDWGTALAFHLAARRPQRVLGLAFMEFIRPFERWEDFHQRPQAREMFKALRT
PGVGEKLVLEDNVFVEKVLPASVLRAMSDDEMDVYRAPFPTPQSRKPVLRLPREMPIEGQPADVAAISAHDHRALRLSTY
PKLLFAGDPGALIGPQAAREFAAGLKNCSFINLGPGAHYLQEDHADAIGRAIASWLPEVVLANQTDELA

Sequences:

>Translated_309_residues
MSSKANPPQPVATAPKRSQIPILDSTMSYVEAGASGPTVLFLHGNPTSSHIWRNIIPHVAPFGRCIAPDLIGYGQSGKPD
IDYRFFDHVRYLDAFLDALDIRDVLLVAQDWGTALAFHLAARRPQRVLGLAFMEFIRPFERWEDFHQRPQAREMFKALRT
PGVGEKLVLEDNVFVEKVLPASVLRAMSDDEMDVYRAPFPTPQSRKPVLRLPREMPIEGQPADVAAISAHDHRALRLSTY
PKLLFAGDPGALIGPQAAREFAAGLKNCSFINLGPGAHYLQEDHADAIGRAIASWLPEVVLANQTDELA
>Mature_308_residues
SSKANPPQPVATAPKRSQIPILDSTMSYVEAGASGPTVLFLHGNPTSSHIWRNIIPHVAPFGRCIAPDLIGYGQSGKPDI
DYRFFDHVRYLDAFLDALDIRDVLLVAQDWGTALAFHLAARRPQRVLGLAFMEFIRPFERWEDFHQRPQAREMFKALRTP
GVGEKLVLEDNVFVEKVLPASVLRAMSDDEMDVYRAPFPTPQSRKPVLRLPREMPIEGQPADVAAISAHDHRALRLSTYP
KLLFAGDPGALIGPQAAREFAAGLKNCSFINLGPGAHYLQEDHADAIGRAIASWLPEVVLANQTDELA

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily

Homologues:

Organism=Caenorhabditis elegans, GI17562298, Length=291, Percent_Identity=21.6494845360825, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324392, Length=106, Percent_Identity=42.4528301886792, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHAA_RHILO (Q98C03)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106032.1
- ProteinModelPortal:   Q98C03
- SMR:   Q98C03
- GeneID:   1228693
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5354
- NMPDR:   fig|266835.1.peg.4136
- HOGENOM:   HBG449717
- OMA:   SHIWRNI
- ProtClustDB:   PRK03592
- BRENDA:   3.8.1.5
- HAMAP:   MF_01231
- InterPro:   IPR000073
- InterPro:   IPR000639
- PRINTS:   PR00412

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.8.1.5

Molecular weight: Translated: 34202; Mature: 34070

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: ACT_SITE 110-110 ACT_SITE 134-134 ACT_SITE 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKANPPQPVATAPKRSQIPILDSTMSYVEAGASGPTVLFLHGNPTSSHIWRNIIPHVA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
PFGRCIAPDLIGYGQSGKPDIDYRFFDHVRYLDAFLDALDIRDVLLVAQDWGTALAFHLA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRPQRVLGLAFMEFIRPFERWEDFHQRPQAREMFKALRTPGVGEKLVLEDNVFVEKVLP
HCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
ASVLRAMSDDEMDVYRAPFPTPQSRKPVLRLPREMPIEGQPADVAAISAHDHRALRLSTY
HHHHHHHCCCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECC
PKLLFAGDPGALIGPQAAREFAAGLKNCSFINLGPGAHYLQEDHADAIGRAIASWLPEVV
CEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LANQTDELA
HCCCHHCCC
>Mature Secondary Structure 
SSKANPPQPVATAPKRSQIPILDSTMSYVEAGASGPTVLFLHGNPTSSHIWRNIIPHVA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
PFGRCIAPDLIGYGQSGKPDIDYRFFDHVRYLDAFLDALDIRDVLLVAQDWGTALAFHLA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRPQRVLGLAFMEFIRPFERWEDFHQRPQAREMFKALRTPGVGEKLVLEDNVFVEKVLP
HCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
ASVLRAMSDDEMDVYRAPFPTPQSRKPVLRLPREMPIEGQPADVAAISAHDHRALRLSTY
HHHHHHHCCCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECC
PKLLFAGDPGALIGPQAAREFAAGLKNCSFINLGPGAHYLQEDHADAIGRAIASWLPEVV
CEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LANQTDELA
HCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968