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The map label for this gene is gatA [H]

Identifier: 13474461

GI number: 13474461

Start: 4271441

End: 4272814

Strand: Direct

Name: gatA [H]

Synonym: mlr5350

Alternate gene names: 13474461

Gene position: 4271441-4272814 (Clockwise)

Preceding gene: 13474460

Following gene: 13474463

Centisome position: 60.71

GC content: 66.59

Gene sequence:

>1374_bases
ATGAATCCCGTCTTCTCCTCGACCATCGAACTCGCCACCGCCATCGCTCGTCGCAAAATCTCTGCCGTCGAAGCACTCGA
TGCCCATCTGGCGCAGATCGACCGGCACAACGAAGGCGTCAACGCCGTCATCTCGCTCGACAGGGAGGGCGCGTACGAAT
GCGCCAGGAAGGCCGACGCGGCACTCGCGCGCGGCGCCACGCCCGGGCCGCTGCACGGCGTTCCCTTCACGCTGAAGGAC
ATGCACGAGACATCTGGCATGAAAACCACGGTCGGCTTCCCGCCCTTCGCCGACTATGTCGCGAGCCACGACAGCCCGGT
CGTTGCGAGACTGAAGGCGGCCGGCGGTGTGCTGATGGCCAAGACCAATGTCGCCACTATGCTGTCGGACTGGCAGTCGA
ACAATCCGCTGTTCGGGCGCACCAGCAATCCATGGAACCTTGAGCGTACGGCGGGTGGCTCCAGCGGCGGCGCCGCGGCG
GCGGTGGCGGCCGCCATGACTCCGTTCGATGTCGGCACCGACATGCAGGATTCGATCCGGCTGCCGGCCGCCTTCTGCGG
CGTCTACGGCCTGAAGCCGACCGAACACCGCGTCTCGCTGGCCGGCGCCTTCCCAAATCCAGGCGATGCCGCGCGCAGCG
TGCGGCTGATGTCCTGCCTCGGCCCGCTGGCGCGCGGCGTAGACGATCTGTCGCTGATCTATCAAATCATCGCCGGGCCG
GATGGACGAGACACCGATCTGGCGCCGGTGCCGGTCGTGGCCATGCCGAAGCTCAACCTGAAGACATTGCGCATCGCCTT
TGCGCCGTCCTTCCCCGGCTTCCCGGTGGCCGGCGATATCGGCGTTGCGGTCGAAAAGCTCGCAAGGCAGTTGCAAGACG
CTGGTGCGATCGTCGCGGATGCGAAACTGCCCAACCTCGATCTGCGCGACGATCTCGCGCAAGGCGGGGCGCTGATCGGC
GTGATGATGGAGGCCGCACAGCCGGAGCCGCCGGAGCGGCCGACACCGGTCTCGCGCTGGTTCGAAGCGCTCGCCCGCCG
CGACCGATCCATTCTCACCTGGGACCAGTTCTTCGACAATTATGACGCGCTGCTTTGCCCGGTGGCGATGACCACGGCCT
TTCCGCATTGCGAGCCGGGTACACCGATCAAGGTCGACGGCAAGGATCAGGACTACTGGATGCTGCCGGCCTATGGCGCC
GTCTTCAACTACAGCGGCCATCCGGCACTTTCGATGCCGTGCGGAGAAGATCGCGACGGCCTGCCGATCGGCCTGCAACT
CGTCGGCAAACGCTGGTCGGAGGCGCGGCTGCTCGGTGTCGCGGCGGCGATCGAGCCGCTCACCGGCGGCTTTCGCCGTC
CGCCCGGCTACTGA

Upstream 100 bases:

>100_bases
GATCAAACCCAGCCAGCCTTCCAATGCTCAACTCCGCTTGAAGCCGCGCCGCTCTCCGCCATCTTGATCGGATGAGCGCG
GCCAAGCCGGAGCCTTGATC

Downstream 100 bases:

>100_bases
GCAAAGCGCGACAATCCATAGGGGGCAAGCAGCGGGTCGCGCTCGCCGTCGAGGATTTCCCGGGCCGCAAACAGGCCGAC
GGCTGGCGCCAGCGTGATGC

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNPVFSSTIELATAIARRKISAVEALDAHLAQIDRHNEGVNAVISLDREGAYECARKADAALARGATPGPLHGVPFTLKD
MHETSGMKTTVGFPPFADYVASHDSPVVARLKAAGGVLMAKTNVATMLSDWQSNNPLFGRTSNPWNLERTAGGSSGGAAA
AVAAAMTPFDVGTDMQDSIRLPAAFCGVYGLKPTEHRVSLAGAFPNPGDAARSVRLMSCLGPLARGVDDLSLIYQIIAGP
DGRDTDLAPVPVVAMPKLNLKTLRIAFAPSFPGFPVAGDIGVAVEKLARQLQDAGAIVADAKLPNLDLRDDLAQGGALIG
VMMEAAQPEPPERPTPVSRWFEALARRDRSILTWDQFFDNYDALLCPVAMTTAFPHCEPGTPIKVDGKDQDYWMLPAYGA
VFNYSGHPALSMPCGEDRDGLPIGLQLVGKRWSEARLLGVAAAIEPLTGGFRRPPGY

Sequences:

>Translated_457_residues
MNPVFSSTIELATAIARRKISAVEALDAHLAQIDRHNEGVNAVISLDREGAYECARKADAALARGATPGPLHGVPFTLKD
MHETSGMKTTVGFPPFADYVASHDSPVVARLKAAGGVLMAKTNVATMLSDWQSNNPLFGRTSNPWNLERTAGGSSGGAAA
AVAAAMTPFDVGTDMQDSIRLPAAFCGVYGLKPTEHRVSLAGAFPNPGDAARSVRLMSCLGPLARGVDDLSLIYQIIAGP
DGRDTDLAPVPVVAMPKLNLKTLRIAFAPSFPGFPVAGDIGVAVEKLARQLQDAGAIVADAKLPNLDLRDDLAQGGALIG
VMMEAAQPEPPERPTPVSRWFEALARRDRSILTWDQFFDNYDALLCPVAMTTAFPHCEPGTPIKVDGKDQDYWMLPAYGA
VFNYSGHPALSMPCGEDRDGLPIGLQLVGKRWSEARLLGVAAAIEPLTGGFRRPPGY
>Mature_457_residues
MNPVFSSTIELATAIARRKISAVEALDAHLAQIDRHNEGVNAVISLDREGAYECARKADAALARGATPGPLHGVPFTLKD
MHETSGMKTTVGFPPFADYVASHDSPVVARLKAAGGVLMAKTNVATMLSDWQSNNPLFGRTSNPWNLERTAGGSSGGAAA
AVAAAMTPFDVGTDMQDSIRLPAAFCGVYGLKPTEHRVSLAGAFPNPGDAARSVRLMSCLGPLARGVDDLSLIYQIIAGP
DGRDTDLAPVPVVAMPKLNLKTLRIAFAPSFPGFPVAGDIGVAVEKLARQLQDAGAIVADAKLPNLDLRDDLAQGGALIG
VMMEAAQPEPPERPTPVSRWFEALARRDRSILTWDQFFDNYDALLCPVAMTTAFPHCEPGTPIKVDGKDQDYWMLPAYGA
VFNYSGHPALSMPCGEDRDGLPIGLQLVGKRWSEARLLGVAAAIEPLTGGFRRPPGY

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=318, Percent_Identity=30.8176100628931, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=27.037773359841, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI166795287, Length=211, Percent_Identity=34.5971563981043, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=494, Percent_Identity=27.7327935222672, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17556264, Length=229, Percent_Identity=33.6244541484716, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538252, Length=193, Percent_Identity=33.6787564766839, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17556276, Length=303, Percent_Identity=31.3531353135314, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556278, Length=303, Percent_Identity=31.3531353135314, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=274, Percent_Identity=28.1021897810219, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=421, Percent_Identity=24.4655581947743, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=193, Percent_Identity=32.1243523316062, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=28.1725888324873, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320448, Length=234, Percent_Identity=32.4786324786325, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644968, Length=508, Percent_Identity=29.1338582677165, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45550774, Length=499, Percent_Identity=27.2545090180361, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24648435, Length=499, Percent_Identity=27.2545090180361, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24648437, Length=499, Percent_Identity=27.2545090180361, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=27.3267326732673, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=27.3267326732673, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=27.3267326732673, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=27.3267326732673, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI21356731, Length=482, Percent_Identity=27.5933609958506, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=27.3092369477912, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648441, Length=434, Percent_Identity=27.1889400921659, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24648439, Length=434, Percent_Identity=27.1889400921659, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24648113, Length=467, Percent_Identity=28.6937901498929, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 48491; Mature: 48491

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVFSSTIELATAIARRKISAVEALDAHLAQIDRHNEGVNAVISLDREGAYECARKADA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
ALARGATPGPLHGVPFTLKDMHETSGMKTTVGFPPFADYVASHDSPVVARLKAAGGVLMA
HHHCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHCCCCCHHHHHHHCCCEEEE
KTNVATMLSDWQSNNPLFGRTSNPWNLERTAGGSSGGAAAAVAAAMTPFDVGTDMQDSIR
ECHHHHHHHHHCCCCCEECCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHC
LPAAFCGVYGLKPTEHRVSLAGAFPNPGDAARSVRLMSCLGPLARGVDDLSLIYQIIAGP
CCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DGRDTDLAPVPVVAMPKLNLKTLRIAFAPSFPGFPVAGDIGVAVEKLARQLQDAGAIVAD
CCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
AKLPNLDLRDDLAQGGALIGVMMEAAQPEPPERPTPVSRWFEALARRDRSILTWDQFFDN
CCCCCCCHHHHHHCCCCEEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHCC
YDALLCPVAMTTAFPHCEPGTPIKVDGKDQDYWMLPAYGAVFNYSGHPALSMPCGEDRDG
CCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCCEEECCCCCEEECCCCCCCCC
LPIGLQLVGKRWSEARLLGVAAAIEPLTGGFRRPPGY
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNPVFSSTIELATAIARRKISAVEALDAHLAQIDRHNEGVNAVISLDREGAYECARKADA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
ALARGATPGPLHGVPFTLKDMHETSGMKTTVGFPPFADYVASHDSPVVARLKAAGGVLMA
HHHCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHCCCCCHHHHHHHCCCEEEE
KTNVATMLSDWQSNNPLFGRTSNPWNLERTAGGSSGGAAAAVAAAMTPFDVGTDMQDSIR
ECHHHHHHHHHCCCCCEECCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHC
LPAAFCGVYGLKPTEHRVSLAGAFPNPGDAARSVRLMSCLGPLARGVDDLSLIYQIIAGP
CCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DGRDTDLAPVPVVAMPKLNLKTLRIAFAPSFPGFPVAGDIGVAVEKLARQLQDAGAIVAD
CCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
AKLPNLDLRDDLAQGGALIGVMMEAAQPEPPERPTPVSRWFEALARRDRSILTWDQFFDN
CCCCCCCHHHHHHCCCCEEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHCC
YDALLCPVAMTTAFPHCEPGTPIKVDGKDQDYWMLPAYGAVFNYSGHPALSMPCGEDRDG
CCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCCEEECCCCCEEECCCCCCCCC
LPIGLQLVGKRWSEARLLGVAAAIEPLTGGFRRPPGY
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA