Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 13474233

GI number: 13474233

Start: 4017917

End: 4019260

Strand: Direct

Name: folC [H]

Synonym: mlr5076

Alternate gene names: 13474233

Gene position: 4017917-4019260 (Clockwise)

Preceding gene: 13474232

Following gene: 13474234

Centisome position: 57.1

GC content: 66.0

Gene sequence:

>1344_bases
ATGCGCCGGGATTTAGCCATGACAACGCTTGCCGCCGACCGCGAAATCGAAGCCCTGATGGCGCTTCATCCGAAAGGTTT
CGACCTTTCGCTCGACCGCATCACGCGGCTCTTGGAGCGGCTTGGCAATCCGCAGGACCTGCTGCCGCCGGTCATCCACA
TCGCCGGCACCAACGGCAAGGGTTCCTGCGCCGCCTTTTCCCGCGCGCTGCTCGAAGCCGCCGGCCACCTAGTCCATGTC
CACACTTCGCCGCACCTGGTGAACTGGGCCGAGCGCTACCGGCTGGCCGCTGAAGGCGGCGGCAAACTCGTCGACGATGA
GACCTTCGCCGAGGCCATTGCCCGCGTCGCCAAGGCCAATGACGGCCAGAAGATCACCGTCTTCGAGATCCTCACCGCCG
TCACCTTCATCCTGTTTTCCGAACATCCGGCCGAAGCCGCCATCATCGAGGTCGGCCTCGGTGGCCGCTTCGATGCCACC
AATGTTGTCACCAGGCCCGCCGTGACCGTGATCATGCCGGTGTCGATGGACCATGAAGCCTATCTCGGCGACCGGGTCGA
ACTGATCGCCGCCGAAAAAGCCGGCATCATGAAGCGCGGCTGCCCGGTGGTCATCGGCGCGCAGGAAAGCGAGACGGCGC
TGCAGGTGCTGATCGAGACCGCCGAGCGGCTGGAGTGCCCGACCTTCGTCTATGGCCAGGATTTCCTCGCCTTCGAAGAA
AACGGCCGCATGGTCTACCAGGACGAGGATGGGTTGATGGATTTGCCGCCGCCGCGTCTGCCGGGACGGCATCAGTTCGC
CAATGCCGCCGCGGCGATCGCCGCGGTCAAGGCGGCCGGCTTCGAGATCAGCCACCGCGCCGCCGAGAAGGCGATGACCC
ATGTCGCCTGGCCCGGCCGCATGCAGAAATTGGCGCAGGGTCGGCTGACGGATCTGGCGCCGAAGGGCGCCGACATCTGG
CTCGACGGCGGCCACAATCCGGGCGCCGGCGTGGTCGTCGCCGAAGCGCTGGCCGAACAGGAAGAAAAGAACCCGCGGCC
GCTGTTCCTCATTTCGGGCATGATCAACACCAAGGACCAGAGTGGCTATTTCCGCGCCTTCAAGGGCCTCGTCCGGCATG
TCTATACGGTGCCGGTGAGCCTGAGCGATGCCGGCGTGCCGAACGACGAACTGGCGATCCGGGCCACGGAAGCCGGGCTG
ACCGCCGAGCCGGTGAGTTCCGTCGCCAACGCGCTGATGCTGCTGCGCGACACATGGGACGGCCCGCCGCCCCGCATCCT
GATCGGCGGATCACTCTATCTTGCCGGCGCGGTGCTAGCCGAGAATGGCACGCCGCCGACCTGA

Upstream 100 bases:

>100_bases
GCGACATCAACCGCCGCGAACAGCGATTGCGCCATGTCCATGGCGCGCTGTAGCCTTTTGATTGCCATGGCCATTCCGGA
TTGATTCTGGTGCTCGGGCC

Downstream 100 bases:

>100_bases
GCGCTTGATGACCGACGCAGGAAGCCCGTGATGATCAACGTGAAGCAGCTCGCCCATGTCTGTATCTTCGCGCATGATCT
GGAAGCGACGCGAAGCTTCT

Product: folylpolyglutamate synthase (folC)

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRRDLAMTTLAADREIEALMALHPKGFDLSLDRITRLLERLGNPQDLLPPVIHIAGTNGKGSCAAFSRALLEAAGHLVHV
HTSPHLVNWAERYRLAAEGGGKLVDDETFAEAIARVAKANDGQKITVFEILTAVTFILFSEHPAEAAIIEVGLGGRFDAT
NVVTRPAVTVIMPVSMDHEAYLGDRVELIAAEKAGIMKRGCPVVIGAQESETALQVLIETAERLECPTFVYGQDFLAFEE
NGRMVYQDEDGLMDLPPPRLPGRHQFANAAAAIAAVKAAGFEISHRAAEKAMTHVAWPGRMQKLAQGRLTDLAPKGADIW
LDGGHNPGAGVVVAEALAEQEEKNPRPLFLISGMINTKDQSGYFRAFKGLVRHVYTVPVSLSDAGVPNDELAIRATEAGL
TAEPVSSVANALMLLRDTWDGPPPRILIGGSLYLAGAVLAENGTPPT

Sequences:

>Translated_447_residues
MRRDLAMTTLAADREIEALMALHPKGFDLSLDRITRLLERLGNPQDLLPPVIHIAGTNGKGSCAAFSRALLEAAGHLVHV
HTSPHLVNWAERYRLAAEGGGKLVDDETFAEAIARVAKANDGQKITVFEILTAVTFILFSEHPAEAAIIEVGLGGRFDAT
NVVTRPAVTVIMPVSMDHEAYLGDRVELIAAEKAGIMKRGCPVVIGAQESETALQVLIETAERLECPTFVYGQDFLAFEE
NGRMVYQDEDGLMDLPPPRLPGRHQFANAAAAIAAVKAAGFEISHRAAEKAMTHVAWPGRMQKLAQGRLTDLAPKGADIW
LDGGHNPGAGVVVAEALAEQEEKNPRPLFLISGMINTKDQSGYFRAFKGLVRHVYTVPVSLSDAGVPNDELAIRATEAGL
TAEPVSSVANALMLLRDTWDGPPPRILIGGSLYLAGAVLAENGTPPT
>Mature_447_residues
MRRDLAMTTLAADREIEALMALHPKGFDLSLDRITRLLERLGNPQDLLPPVIHIAGTNGKGSCAAFSRALLEAAGHLVHV
HTSPHLVNWAERYRLAAEGGGKLVDDETFAEAIARVAKANDGQKITVFEILTAVTFILFSEHPAEAAIIEVGLGGRFDAT
NVVTRPAVTVIMPVSMDHEAYLGDRVELIAAEKAGIMKRGCPVVIGAQESETALQVLIETAERLECPTFVYGQDFLAFEE
NGRMVYQDEDGLMDLPPPRLPGRHQFANAAAAIAAVKAAGFEISHRAAEKAMTHVAWPGRMQKLAQGRLTDLAPKGADIW
LDGGHNPGAGVVVAEALAEQEEKNPRPLFLISGMINTKDQSGYFRAFKGLVRHVYTVPVSLSDAGVPNDELAIRATEAGL
TAEPVSSVANALMLLRDTWDGPPPRILIGGSLYLAGAVLAENGTPPT

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=329, Percent_Identity=28.8753799392097, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI66932984, Length=329, Percent_Identity=28.8753799392097, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1788654, Length=368, Percent_Identity=32.3369565217391, Blast_Score=138, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17553148, Length=465, Percent_Identity=25.3763440860215, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=465, Percent_Identity=25.3763440860215, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=465, Percent_Identity=25.3763440860215, Blast_Score=121, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=432, Percent_Identity=29.6296296296296, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324815, Length=341, Percent_Identity=26.099706744868, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322718, Length=291, Percent_Identity=24.7422680412371, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24641571, Length=190, Percent_Identity=31.5789473684211, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24581568, Length=240, Percent_Identity=28.3333333333333, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47913; Mature: 47913

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRDLAMTTLAADREIEALMALHPKGFDLSLDRITRLLERLGNPQDLLPPVIHIAGTNGK
CCCCHHHHHHHCCHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCHHCCCCEEEEECCCCC
GSCAAFSRALLEAAGHLVHVHTSPHLVNWAERYRLAAEGGGKLVDDETFAEAIARVAKAN
CHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHCC
DGQKITVFEILTAVTFILFSEHPAEAAIIEVGLGGRFDATNVVTRPAVTVIMPVSMDHEA
CCCEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHH
YLGDRVELIAAEKAGIMKRGCPVVIGAQESETALQVLIETAERLECPTFVYGQDFLAFEE
HCCCCEEEEEEHHCCHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC
NGRMVYQDEDGLMDLPPPRLPGRHQFANAAAAIAAVKAAGFEISHRAAEKAMTHVAWPGR
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
MQKLAQGRLTDLAPKGADIWLDGGHNPGAGVVVAEALAEQEEKNPRPLFLISGMINTKDQ
HHHHHCCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
SGYFRAFKGLVRHVYTVPVSLSDAGVPNDELAIRATEAGLTAEPVSSVANALMLLRDTWD
CCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
GPPPRILIGGSLYLAGAVLAENGTPPT
CCCCEEEECCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MRRDLAMTTLAADREIEALMALHPKGFDLSLDRITRLLERLGNPQDLLPPVIHIAGTNGK
CCCCHHHHHHHCCHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCHHCCCCEEEEECCCCC
GSCAAFSRALLEAAGHLVHVHTSPHLVNWAERYRLAAEGGGKLVDDETFAEAIARVAKAN
CHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHCC
DGQKITVFEILTAVTFILFSEHPAEAAIIEVGLGGRFDATNVVTRPAVTVIMPVSMDHEA
CCCEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHH
YLGDRVELIAAEKAGIMKRGCPVVIGAQESETALQVLIETAERLECPTFVYGQDFLAFEE
HCCCCEEEEEEHHCCHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC
NGRMVYQDEDGLMDLPPPRLPGRHQFANAAAAIAAVKAAGFEISHRAAEKAMTHVAWPGR
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
MQKLAQGRLTDLAPKGADIWLDGGHNPGAGVVVAEALAEQEEKNPRPLFLISGMINTKDQ
HHHHHCCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
SGYFRAFKGLVRHVYTVPVSLSDAGVPNDELAIRATEAGLTAEPVSSVANALMLLRDTWD
CCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
GPPPRILIGGSLYLAGAVLAENGTPPT
CCCCEEEECCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]