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The map label for this gene is pheS

Identifier: 13474218

GI number: 13474218

Start: 4006476

End: 4007573

Strand: Reverse

Name: pheS

Synonym: mll5055

Alternate gene names: 13474218

Gene position: 4007573-4006476 (Counterclockwise)

Preceding gene: 13474219

Following gene: 13474217

Centisome position: 56.96

GC content: 63.57

Gene sequence:

>1098_bases
GTGAACGACGCAACTAGTGGCCTGGATACGCTCGAAAATTCCCTGCTGGCCGATATTGCATCGGCGGCCGATGAGCCGGC
GATCGAGGCCGTGCGCATAGCCGCCTTCGGCAAGAAGGGCACCGTGTCCGAGATGCTGAAAACGCTTGGCGCCATGACCG
CCGAGGAGCGCCAGCTCAAGGGGCCCGTCATCAACGGCCTCAAGAACCGCGTCACCGAGGCGCTGACGGCACGCAAGGCC
GAATTGAAAGACGTGGCGATCGCAGCACGTCTCGCCGCCGAGAAGGTCGACGTGACGCTGCCGGTGCGGCAGTCGCCAGC
CGAGCGCGGCCGCATCCATCCGATCAGCCAGGTCATCGACGAGATCGCCGCGATCTTCGGCGATCTCGGCTTCGCCATCG
CAGAAGGTCCAGACATCGAGACCGACTATTACAATTTCACCGCGCTGAATTTCCCCGAGGGACATCCGGCGCGCGAGATG
CACGACACCTTCTTCTTCCAGCCGGACGAGAAGGGCGAGCGCAAGCTGTTGCGCACGCACACCTCGCCCGTGCAGATCCG
CACCATGGAGAAGCAGAAGCCGCCGATCCGCATCGTCATTCCGGGCAAGACCTACCGCCAGGATTCCGACGCCACGCACT
CGCCGATGTTCCATCAGGTCGAGGGACTGGTGATCGACAAGACAGCCAATGTCGCCAACATGAAGTGGGTGCTGGAGGAA
TTCTGCAAGGCGTTCTTCGAGGTACCCTCGGTCAAGATGCGCTTCCGGCCGTCCTTCTTCCCGTTCACCGAGCCAAGCCT
CGAGGTCGACATACAGTGCGACCGCTCGCGGCCCGGCGAAGTGCGCTTCGGTGAAGGCTCCGACTGGATGGAGATCCTCG
GCTGCGGCATGGTGCATCCCAATGTGCTGCGCGCCGGCGGGCTCGATCCGGACGAGTACCAGGGCTTTGCCTGGGGCATG
GGCATCGACCGCATCGCCATGCTGAAATACGGCATGCCAGACCTGCGCGCCTTCTTCGACGCCGATGTGCGCTGGCTGTC
GCATTACGGCTTCCGGCCGCTCGACATGCCGACGCTGTTCGGCGGCTTGAGCGCATGA

Upstream 100 bases:

>100_bases
GGCACGGCTGGCGCGGGTTCTTTTGTCTCTGATGTCTGAACTTTCAACTTAAAAGTGACTTCCACTTTAAAGTCGACTTC
CAACGTAGCGAGTTTGACCC

Downstream 100 bases:

>100_bases
CAGCGCTCCACATCGGCGGCTGCCGCTGCGGCGCCGTGCGGTTCGAGGCCTCGGCCGAGCCGCATCACGTCAGCTATTGC
CATTGCGGCGATTGCCGGCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNDATSGLDTLENSLLADIASAADEPAIEAVRIAAFGKKGTVSEMLKTLGAMTAEERQLKGPVINGLKNRVTEALTARKA
ELKDVAIAARLAAEKVDVTLPVRQSPAERGRIHPISQVIDEIAAIFGDLGFAIAEGPDIETDYYNFTALNFPEGHPAREM
HDTFFFQPDEKGERKLLRTHTSPVQIRTMEKQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKTANVANMKWVLEE
FCKAFFEVPSVKMRFRPSFFPFTEPSLEVDIQCDRSRPGEVRFGEGSDWMEILGCGMVHPNVLRAGGLDPDEYQGFAWGM
GIDRIAMLKYGMPDLRAFFDADVRWLSHYGFRPLDMPTLFGGLSA

Sequences:

>Translated_365_residues
MNDATSGLDTLENSLLADIASAADEPAIEAVRIAAFGKKGTVSEMLKTLGAMTAEERQLKGPVINGLKNRVTEALTARKA
ELKDVAIAARLAAEKVDVTLPVRQSPAERGRIHPISQVIDEIAAIFGDLGFAIAEGPDIETDYYNFTALNFPEGHPAREM
HDTFFFQPDEKGERKLLRTHTSPVQIRTMEKQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKTANVANMKWVLEE
FCKAFFEVPSVKMRFRPSFFPFTEPSLEVDIQCDRSRPGEVRFGEGSDWMEILGCGMVHPNVLRAGGLDPDEYQGFAWGM
GIDRIAMLKYGMPDLRAFFDADVRWLSHYGFRPLDMPTLFGGLSA
>Mature_365_residues
MNDATSGLDTLENSLLADIASAADEPAIEAVRIAAFGKKGTVSEMLKTLGAMTAEERQLKGPVINGLKNRVTEALTARKA
ELKDVAIAARLAAEKVDVTLPVRQSPAERGRIHPISQVIDEIAAIFGDLGFAIAEGPDIETDYYNFTALNFPEGHPAREM
HDTFFFQPDEKGERKLLRTHTSPVQIRTMEKQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKTANVANMKWVLEE
FCKAFFEVPSVKMRFRPSFFPFTEPSLEVDIQCDRSRPGEVRFGEGSDWMEILGCGMVHPNVLRAGGLDPDEYQGFAWGM
GIDRIAMLKYGMPDLRAFFDADVRWLSHYGFRPLDMPTLFGGLSA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=278, Percent_Identity=30.2158273381295, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI5729820, Length=294, Percent_Identity=24.4897959183673, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=50.8720930232558, Blast_Score=354, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=29.0909090909091, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=29.0909090909091, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=30.0380228136882, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=275, Percent_Identity=28.7272727272727, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=94, Percent_Identity=36.1702127659575, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858079, Length=274, Percent_Identity=30.6569343065693, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137424, Length=300, Percent_Identity=25.3333333333333, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_RHILO (Q98CP9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105786.1
- ProteinModelPortal:   Q98CP9
- GeneID:   1228447
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5055
- NMPDR:   fig|266835.1.peg.3890
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40607; Mature: 40607

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDATSGLDTLENSLLADIASAADEPAIEAVRIAAFGKKGTVSEMLKTLGAMTAEERQLK
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCHHHHHHHHHCCCHHHHHHC
GPVINGLKNRVTEALTARKAELKDVAIAARLAAEKVDVTLPVRQSPAERGRIHPISQVID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCCCHHHHHHH
EIAAIFGDLGFAIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFQPDEKGERKLLRTH
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHEECCCCHHHHHHHHHC
TSPVQIRTMEKQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKTANVANMKWVLEE
CCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHH
FCKAFFEVPSVKMRFRPSFFPFTEPSLEVDIQCDRSRPGEVRFGEGSDWMEILGCGMVHP
HHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHCCCCCC
NVLRAGGLDPDEYQGFAWGMGIDRIAMLKYGMPDLRAFFDADVRWLSHYGFRPLDMPTLF
CHHHCCCCCHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GGLSA
CCCCC
>Mature Secondary Structure
MNDATSGLDTLENSLLADIASAADEPAIEAVRIAAFGKKGTVSEMLKTLGAMTAEERQLK
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCHHHHHHHHHCCCHHHHHHC
GPVINGLKNRVTEALTARKAELKDVAIAARLAAEKVDVTLPVRQSPAERGRIHPISQVID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCCCHHHHHHH
EIAAIFGDLGFAIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFQPDEKGERKLLRTH
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHEECCCCHHHHHHHHHC
TSPVQIRTMEKQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKTANVANMKWVLEE
CCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHH
FCKAFFEVPSVKMRFRPSFFPFTEPSLEVDIQCDRSRPGEVRFGEGSDWMEILGCGMVHP
HHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHCCCCCC
NVLRAGGLDPDEYQGFAWGMGIDRIAMLKYGMPDLRAFFDADVRWLSHYGFRPLDMPTLF
CHHHCCCCCHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GGLSA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968