| Definition | Mesorhizobium loti MAFF303099 chromosome, complete genome. |
|---|---|
| Accession | NC_002678 |
| Length | 7,036,071 |
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The map label for this gene is idnO [C]
Identifier: 13474166
GI number: 13474166
Start: 3957177
End: 3957971
Strand: Reverse
Name: idnO [C]
Synonym: mll4989
Alternate gene names: 13474166
Gene position: 3957971-3957177 (Counterclockwise)
Preceding gene: 13474167
Following gene: 13474165
Centisome position: 56.25
GC content: 64.03
Gene sequence:
>795_bases ATGAGCGCATCTTCCGATCGCAATCATGAGACACGCGCCATCGTCACCGGTGGCGCGCAAGGCATCGGCTTTGCCGTCGC CGAGGCGCTGGCCGACGAGGGCTGCCGGGCATTGGCGCTCATCGGCCGTTCGCAGGAGAAGGGCGACAAGGCCGTCGCCC ACTTCAAAAAGGCCGGCGTCGACGCGATCTTCATCAGCGCCGACGTCTCGAAGGTGGCGGATTGCAAGCGCGCGGTGGCG ACCGCGCTCGCGCATTTCGGCACGCTGAATGCGCTGGTCAACGCCGCCGCCACCTCGGCACGCGGCTCATTGGTCGAAAC CTCGGAAGAGCTTTTCGACCAGATATTCGCAACCAATGTGCGCGGACCTTTCTTCCTGATGCAGGGACTGGTTGCCCATC TGCTGGAGCGCAAGGCGCCGGGCTCGATCGTCAACGTGCTGTCGATGTCGGCACATTGTGGCCAGTCGTTCCTGACGCCC TATTCGACCAGCAAGGGCGCGCTGATGACGCTGACCAAGAACGTCGCCAACGCCTATCGCTTCGACCGCATCCGCTGCAA CGCCGTGCTGCCCGGCTGGATGGATACCGAGGGCGAGGACATCGTGCAAAAGAAATGGCACGACGCCCCGGACGATTGGC TGGCGAAGGCCGAAGCCGCGCAACCGATGGGCCAATTGGTCAAACCGGACCAGCTCGCGCGGCTGATCAGCTACATGGTC AGCCCGCAATCGGGCGTCATGACCGGGTCGTTGGTCGACTACGACCAGAGTGTTGCGGGGTCGTCGCCGGAGTAG
Upstream 100 bases:
>100_bases AAGCTGGCGGATGCGGCGCTGAAGTCGGCGACAACAGGCAAGACCGTTCGTCTCGACAAGTAAGAACCGGAATCCAGGCC CAAGCCACAGGAGCAAAACG
Downstream 100 bases:
>100_bases GGCCGACGGTCTGGCGGAAATGCTCCTCACTTCGTCATCCACGGGCGGGGCAAGAAGCGAAGCGACGCAGCGCAGACCCG AGGATCCGCCGCACTCCACG
Product: short chain dehydrogenase
Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]
Alternate protein names: NA
Number of amino acids: Translated: 264; Mature: 263
Protein sequence:
>264_residues MSASSDRNHETRAIVTGGAQGIGFAVAEALADEGCRALALIGRSQEKGDKAVAHFKKAGVDAIFISADVSKVADCKRAVA TALAHFGTLNALVNAAATSARGSLVETSEELFDQIFATNVRGPFFLMQGLVAHLLERKAPGSIVNVLSMSAHCGQSFLTP YSTSKGALMTLTKNVANAYRFDRIRCNAVLPGWMDTEGEDIVQKKWHDAPDDWLAKAEAAQPMGQLVKPDQLARLISYMV SPQSGVMTGSLVDYDQSVAGSSPE
Sequences:
>Translated_264_residues MSASSDRNHETRAIVTGGAQGIGFAVAEALADEGCRALALIGRSQEKGDKAVAHFKKAGVDAIFISADVSKVADCKRAVA TALAHFGTLNALVNAAATSARGSLVETSEELFDQIFATNVRGPFFLMQGLVAHLLERKAPGSIVNVLSMSAHCGQSFLTP YSTSKGALMTLTKNVANAYRFDRIRCNAVLPGWMDTEGEDIVQKKWHDAPDDWLAKAEAAQPMGQLVKPDQLARLISYMV SPQSGVMTGSLVDYDQSVAGSSPE >Mature_263_residues SASSDRNHETRAIVTGGAQGIGFAVAEALADEGCRALALIGRSQEKGDKAVAHFKKAGVDAIFISADVSKVADCKRAVAT ALAHFGTLNALVNAAATSARGSLVETSEELFDQIFATNVRGPFFLMQGLVAHLLERKAPGSIVNVLSMSAHCGQSFLTPY STSKGALMTLTKNVANAYRFDRIRCNAVLPGWMDTEGEDIVQKKWHDAPDDWLAKAEAAQPMGQLVKPDQLARLISYMVS PQSGVMTGSLVDYDQSVAGSSPE
Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=262, Percent_Identity=30.5343511450382, Blast_Score=109, Evalue=2e-24, Organism=Homo sapiens, GI5031737, Length=238, Percent_Identity=31.5126050420168, Blast_Score=107, Evalue=1e-23, Organism=Homo sapiens, GI33667109, Length=192, Percent_Identity=34.375, Blast_Score=101, Evalue=5e-22, Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=30.078125, Blast_Score=91, Evalue=9e-19, Organism=Homo sapiens, GI40254992, Length=241, Percent_Identity=31.9502074688797, Blast_Score=91, Evalue=2e-18, Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=31.0483870967742, Blast_Score=88, Evalue=8e-18, Organism=Homo sapiens, GI126723750, Length=265, Percent_Identity=27.5471698113208, Blast_Score=88, Evalue=9e-18, Organism=Homo sapiens, GI126723191, Length=179, Percent_Identity=34.0782122905028, Blast_Score=87, Evalue=2e-17, Organism=Homo sapiens, GI4758504, Length=196, Percent_Identity=30.6122448979592, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI66933014, Length=171, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI10190704, Length=245, Percent_Identity=26.9387755102041, Blast_Score=72, Evalue=7e-13, Organism=Homo sapiens, GI59889578, Length=181, Percent_Identity=28.1767955801105, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI19923817, Length=249, Percent_Identity=24.0963855421687, Blast_Score=68, Evalue=6e-12, Organism=Homo sapiens, GI83715985, Length=176, Percent_Identity=31.25, Blast_Score=68, Evalue=8e-12, Organism=Homo sapiens, GI4504505, Length=186, Percent_Identity=28.494623655914, Blast_Score=66, Evalue=3e-11, Organism=Escherichia coli, GI1790717, Length=242, Percent_Identity=28.5123966942149, Blast_Score=101, Evalue=6e-23, Organism=Escherichia coli, GI1789208, Length=243, Percent_Identity=26.7489711934156, Blast_Score=88, Evalue=5e-19, Organism=Escherichia coli, GI1786812, Length=234, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=2e-18, Organism=Escherichia coli, GI87082100, Length=243, Percent_Identity=29.6296296296296, Blast_Score=85, Evalue=4e-18, Organism=Escherichia coli, GI1787335, Length=237, Percent_Identity=24.8945147679325, Blast_Score=78, Evalue=5e-16, Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=29.2, Blast_Score=74, Evalue=7e-15, Organism=Escherichia coli, GI1788459, Length=255, Percent_Identity=25.8823529411765, Blast_Score=69, Evalue=3e-13, Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=26.4822134387352, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI87082160, Length=185, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17561402, Length=255, Percent_Identity=31.3725490196078, Blast_Score=110, Evalue=7e-25, Organism=Caenorhabditis elegans, GI17560676, Length=243, Percent_Identity=29.2181069958848, Blast_Score=92, Evalue=3e-19, Organism=Caenorhabditis elegans, GI25147288, Length=243, Percent_Identity=28.3950617283951, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI115534694, Length=246, Percent_Identity=28.4552845528455, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI71994604, Length=251, Percent_Identity=27.4900398406374, Blast_Score=85, Evalue=5e-17, Organism=Caenorhabditis elegans, GI17562910, Length=255, Percent_Identity=30.1960784313725, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17538182, Length=195, Percent_Identity=32.8205128205128, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI71994600, Length=249, Percent_Identity=28.1124497991968, Blast_Score=82, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17560150, Length=237, Percent_Identity=28.6919831223629, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17551412, Length=243, Percent_Identity=28.8065843621399, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17560220, Length=241, Percent_Identity=29.8755186721992, Blast_Score=77, Evalue=1e-14, Organism=Caenorhabditis elegans, GI17560332, Length=238, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17562906, Length=237, Percent_Identity=29.1139240506329, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI17559104, Length=248, Percent_Identity=29.4354838709677, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17562990, Length=234, Percent_Identity=28.6324786324786, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17561272, Length=194, Percent_Identity=27.319587628866, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI193204405, Length=238, Percent_Identity=28.9915966386555, Blast_Score=72, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17538486, Length=239, Percent_Identity=28.0334728033473, Blast_Score=72, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17555706, Length=241, Percent_Identity=26.1410788381743, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI17531453, Length=237, Percent_Identity=28.6919831223629, Blast_Score=72, Evalue=4e-13, Organism=Caenorhabditis elegans, GI17565030, Length=243, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17536651, Length=253, Percent_Identity=26.4822134387352, Blast_Score=69, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17562908, Length=238, Percent_Identity=28.9915966386555, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17508651, Length=248, Percent_Identity=27.0161290322581, Blast_Score=67, Evalue=7e-12, Organism=Caenorhabditis elegans, GI72000259, Length=241, Percent_Identity=30.2904564315353, Blast_Score=67, Evalue=1e-11, Organism=Caenorhabditis elegans, GI17538480, Length=238, Percent_Identity=27.3109243697479, Blast_Score=66, Evalue=1e-11, Organism=Caenorhabditis elegans, GI17544670, Length=245, Percent_Identity=25.3061224489796, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17563726, Length=234, Percent_Identity=26.4957264957265, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17536025, Length=250, Percent_Identity=25.6, Blast_Score=65, Evalue=2e-11, Organism=Saccharomyces cerevisiae, GI6324126, Length=249, Percent_Identity=27.710843373494, Blast_Score=70, Evalue=3e-13, Organism=Drosophila melanogaster, GI21355319, Length=241, Percent_Identity=33.6099585062241, Blast_Score=115, Evalue=4e-26, Organism=Drosophila melanogaster, GI23397609, Length=245, Percent_Identity=33.8775510204082, Blast_Score=109, Evalue=2e-24, Organism=Drosophila melanogaster, GI24639444, Length=245, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=2e-21, Organism=Drosophila melanogaster, GI21357041, Length=240, Percent_Identity=30, Blast_Score=87, Evalue=1e-17, Organism=Drosophila melanogaster, GI28571526, Length=242, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=2e-16, Organism=Drosophila melanogaster, GI24644339, Length=245, Percent_Identity=28.1632653061224, Blast_Score=75, Evalue=5e-14, Organism=Drosophila melanogaster, GI17737361, Length=256, Percent_Identity=28.515625, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI18859837, Length=187, Percent_Identity=28.3422459893048, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24643142, Length=237, Percent_Identity=29.535864978903, Blast_Score=66, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.69 [C]
Molecular weight: Translated: 28017; Mature: 27886
Theoretical pI: Translated: 6.51; Mature: 6.51
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSASSDRNHETRAIVTGGAQGIGFAVAEALADEGCRALALIGRSQEKGDKAVAHFKKAGV CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCC DAIFISADVSKVADCKRAVATALAHFGTLNALVNAAATSARGSLVETSEELFDQIFATNV CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC RGPFFLMQGLVAHLLERKAPGSIVNVLSMSAHCGQSFLTPYSTSKGALMTLTKNVANAYR CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHH FDRIRCNAVLPGWMDTEGEDIVQKKWHDAPDDWLAKAEAAQPMGQLVKPDQLARLISYMV HHHEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH SPQSGVMTGSLVDYDQSVAGSSPE CCCCCCEECCHHCCCHHCCCCCCC >Mature Secondary Structure SASSDRNHETRAIVTGGAQGIGFAVAEALADEGCRALALIGRSQEKGDKAVAHFKKAGV CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCC DAIFISADVSKVADCKRAVATALAHFGTLNALVNAAATSARGSLVETSEELFDQIFATNV CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC RGPFFLMQGLVAHLLERKAPGSIVNVLSMSAHCGQSFLTPYSTSKGALMTLTKNVANAYR CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHH FDRIRCNAVLPGWMDTEGEDIVQKKWHDAPDDWLAKAEAAQPMGQLVKPDQLARLISYMV HHHEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH SPQSGVMTGSLVDYDQSVAGSSPE CCCCCCEECCHHCCCHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NAD+; NADH; NADP+; NADPH [C]
Metal ions: Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.02 {NADP+}} 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} [C]
Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]
Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]
General reaction: Redox reaction [C]
Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 10360571 [H]