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The map label for this gene is iolD [H]

Identifier: 13474159

GI number: 13474159

Start: 3948083

End: 3950002

Strand: Reverse

Name: iolD [H]

Synonym: mll4981

Alternate gene names: 13474159

Gene position: 3950002-3948083 (Counterclockwise)

Preceding gene: 13474160

Following gene: 13474158

Centisome position: 56.14

GC content: 65.83

Gene sequence:

>1920_bases
GTGGCTTGCCGCGCGCGGCCGCAAGGCGGCATAAGAAGGCGCGGCACAAGGACTGCGGAGGAAACCATCATGAGCAAGAC
AATCCGCCTGACGATGGCGCAGGCGCTGACCCGCTTCCTGTCCCGCCAGATGACCGAGATCGACGGCAAGAAAATGCCGA
TCTTCGGCGGTGTCTGGGCGATCTTCGGCCACGGTAACGTCGCTGGCATGGGCGAGGCGCTCTACCAGGTGCGCGACGAA
CTGCCGACCTTCCGCGCCCACAACGAACAGGCGATGGCGCATGCGGCGATCGCCTATGCCAAGGCCAATTTCCGCCGCCG
CTTCATGGCCGCGACCTCGTCGATCGGTCCCGGCGCGCTCAACATGGTGACGGCGGCGGCACTTGCGCATGTAAACAGAT
TGCCTGTCCTGTTTTTGCCCGGCGACGTCTTCGCCAACCGCATTCCCGATCCGGTGCTGCAGCAGGCCGAGGACTTTTCC
GACGGCACGGCGACGGTCAATGACTGCTTCCGTCCGGTGTCGCGCTATTTCGACCGCATCACGCGGCCCGAGCAGATCAT
TCCGGCGCTGAGCCGTGCCATGCAGGTGCTGACCGATCCGGCCGATTGCGGCCCGGTGACGCTGTCGCTGTGCCAGGATG
TGCAGGCCGAGGCCTATGATTATCCCGAGAGCTTCTTCGCCGAACGGGTCTGGCACCAGCGCCGGCCGCGCCCGGACCGC
CACGAACTCGCCGCCGCTGTGACGGCACTCAAGGGTGCGAAGAAGCCGCTGGTCATCGCCGGCGGCGGCGTTCTCTATTC
GCAGGCCTCCGATGCGCTGGCCAAATTCGTCGAAGGCGCCGGCATCCCGGTCTGCGAGACGCAAGGCGGCAAATCCTCGC
TGCCGGACGATCATCCGCTCAACATGGCGGCGGTCGGCGTGACCGGCACGTCTGCCGCCAACCGGCTGGCGGAAGAAGCC
GATGTCGTGCTCGCCATCGGCACGCGCCTGCAGGATTTCACCACCGGCTCATGGGCGCTGTTCAAGAATTCCGGCAAGAC
CATCATCGGGCTGAACGTCCAACCTTTCGACGCCGGCAAACACCGCGCCCTGCCGCTGGTCGCGGACGCGGCCGAGGGTA
TCGCCGAACTTGGCGCGGCGCTGAAAGGCTGGAAGGCGCCTGCCGGCTGGACCGACAATGCCGCCAAGGGCAAGAAGGCC
TGGCAGGCCGACGCGGCCAAGGTGACGGCGTCGACCAACGCCGCCTTCCCGTCCGACGCGCAGGTGATCGGCGGCGTGCA
GCGGGCCATGGGCTCCGGCGTGACGCTGCTGCATGCCGCCGGCGGTCTGCCCGGCGAATTGCACAAGCTTTGGCAGGCCG
GCACGCCGGGTTCCTACCACGCCGAATACGGTTTCTCGACCATGGGCTACGAGATTGCCGGCGGACTCGGCACCAAGATG
GCCAAGCCCGGCGAAGAGGTGGTCGTCATGATCGGCGACGGCTCCTATCTGATGCTGAATTCGGAGATCGCCACCTCTGT
GATGCTGGGTTTGAAGCTCATCATCGTGCTGCTCGACAACCGTGGCTATGGCTGCATCAATCGGCTGCAGATGGCGACCG
GCGGCGCCAATTTCAACAATCTCCTGAAGGATTCGCGCCACGAAATCCTGCCCGACATCGACTTCGCCGCGCATGCGGCG
AGCATGGGCGCGATCGCCGAGAAGGTGCCGTCGATCGCCGGCCTGGAAAACGCGCTGCAGAAGGCCAAGACAAACGACCG
CACCACGGTGCTGGTCATCGACACCGACCCGCTGGTCTCCACCGATGCCGGCGGTCATTGGTGGGATGTGGCGGTGCCGG
AAGTCTCCGCGCGGCCGCAGGTCATCGCGGCGCGCAAGGCCTATGACGAAAAGCGCCAGATGCAGAGCGTCGGCGATTGA

Upstream 100 bases:

>100_bases
CGATCTTCGTGCACGCGGCCGAAGAGTGGCTGGCCGGCAGGATGTCGGATGACGAGGCTGTCGCCGACATGGCCTCGCGC
TTTGAACAGTTGACCGAGGC

Downstream 100 bases:

>100_bases
TGCCGGCGCTGGCAGCCCCCACCTCCCCCTTGTGGGGAGGTCGGACCGAAGGTCCGGGTGGGGGTCGGCGCCGCACCCCC
ACCCCGCTGCTTCGCAGCGA

Product: acetolactate synthase, iolD

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MACRARPQGGIRRRGTRTAEETIMSKTIRLTMAQALTRFLSRQMTEIDGKKMPIFGGVWAIFGHGNVAGMGEALYQVRDE
LPTFRAHNEQAMAHAAIAYAKANFRRRFMAATSSIGPGALNMVTAAALAHVNRLPVLFLPGDVFANRIPDPVLQQAEDFS
DGTATVNDCFRPVSRYFDRITRPEQIIPALSRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFAERVWHQRRPRPDR
HELAAAVTALKGAKKPLVIAGGGVLYSQASDALAKFVEGAGIPVCETQGGKSSLPDDHPLNMAAVGVTGTSAANRLAEEA
DVVLAIGTRLQDFTTGSWALFKNSGKTIIGLNVQPFDAGKHRALPLVADAAEGIAELGAALKGWKAPAGWTDNAAKGKKA
WQADAAKVTASTNAAFPSDAQVIGGVQRAMGSGVTLLHAAGGLPGELHKLWQAGTPGSYHAEYGFSTMGYEIAGGLGTKM
AKPGEEVVVMIGDGSYLMLNSEIATSVMLGLKLIIVLLDNRGYGCINRLQMATGGANFNNLLKDSRHEILPDIDFAAHAA
SMGAIAEKVPSIAGLENALQKAKTNDRTTVLVIDTDPLVSTDAGGHWWDVAVPEVSARPQVIAARKAYDEKRQMQSVGD

Sequences:

>Translated_639_residues
MACRARPQGGIRRRGTRTAEETIMSKTIRLTMAQALTRFLSRQMTEIDGKKMPIFGGVWAIFGHGNVAGMGEALYQVRDE
LPTFRAHNEQAMAHAAIAYAKANFRRRFMAATSSIGPGALNMVTAAALAHVNRLPVLFLPGDVFANRIPDPVLQQAEDFS
DGTATVNDCFRPVSRYFDRITRPEQIIPALSRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFAERVWHQRRPRPDR
HELAAAVTALKGAKKPLVIAGGGVLYSQASDALAKFVEGAGIPVCETQGGKSSLPDDHPLNMAAVGVTGTSAANRLAEEA
DVVLAIGTRLQDFTTGSWALFKNSGKTIIGLNVQPFDAGKHRALPLVADAAEGIAELGAALKGWKAPAGWTDNAAKGKKA
WQADAAKVTASTNAAFPSDAQVIGGVQRAMGSGVTLLHAAGGLPGELHKLWQAGTPGSYHAEYGFSTMGYEIAGGLGTKM
AKPGEEVVVMIGDGSYLMLNSEIATSVMLGLKLIIVLLDNRGYGCINRLQMATGGANFNNLLKDSRHEILPDIDFAAHAA
SMGAIAEKVPSIAGLENALQKAKTNDRTTVLVIDTDPLVSTDAGGHWWDVAVPEVSARPQVIAARKAYDEKRQMQSVGD
>Mature_638_residues
ACRARPQGGIRRRGTRTAEETIMSKTIRLTMAQALTRFLSRQMTEIDGKKMPIFGGVWAIFGHGNVAGMGEALYQVRDEL
PTFRAHNEQAMAHAAIAYAKANFRRRFMAATSSIGPGALNMVTAAALAHVNRLPVLFLPGDVFANRIPDPVLQQAEDFSD
GTATVNDCFRPVSRYFDRITRPEQIIPALSRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFAERVWHQRRPRPDRH
ELAAAVTALKGAKKPLVIAGGGVLYSQASDALAKFVEGAGIPVCETQGGKSSLPDDHPLNMAAVGVTGTSAANRLAEEAD
VVLAIGTRLQDFTTGSWALFKNSGKTIIGLNVQPFDAGKHRALPLVADAAEGIAELGAALKGWKAPAGWTDNAAKGKKAW
QADAAKVTASTNAAFPSDAQVIGGVQRAMGSGVTLLHAAGGLPGELHKLWQAGTPGSYHAEYGFSTMGYEIAGGLGTKMA
KPGEEVVVMIGDGSYLMLNSEIATSVMLGLKLIIVLLDNRGYGCINRLQMATGGANFNNLLKDSRHEILPDIDFAAHAAS
MGAIAEKVPSIAGLENALQKAKTNDRTTVLVIDTDPLVSTDAGGHWWDVAVPEVSARPQVIAARKAYDEKRQMQSVGD

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=551, Percent_Identity=26.1343012704174, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1790104, Length=509, Percent_Identity=23.7721021611002, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786717, Length=437, Percent_Identity=24.9427917620137, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1788716, Length=503, Percent_Identity=24.6520874751491, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787096, Length=507, Percent_Identity=23.4714003944773, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531301, Length=534, Percent_Identity=26.4044943820225, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=534, Percent_Identity=26.4044943820225, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=628, Percent_Identity=23.4076433121019, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 68051; Mature: 67920

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACRARPQGGIRRRGTRTAEETIMSKTIRLTMAQALTRFLSRQMTEIDGKKMPIFGGVWA
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEE
IFGHGNVAGMGEALYQVRDELPTFRAHNEQAMAHAAIAYAKANFRRRFMAATSSIGPGAL
EEECCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NMVTAAALAHVNRLPVLFLPGDVFANRIPDPVLQQAEDFSDGTATVNDCFRPVSRYFDRI
HHHHHHHHHHHHCCCEEEECCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
TRPEQIIPALSRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFAERVWHQRRPRPDR
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCH
HELAAAVTALKGAKKPLVIAGGGVLYSQASDALAKFVEGAGIPVCETQGGKSSLPDDHPL
HHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
NMAAVGVTGTSAANRLAEEADVVLAIGTRLQDFTTGSWALFKNSGKTIIGLNVQPFDAGK
CEEEEECCCCHHHHHHHHHCCEEEEECCCHHHCCCCCEEEEECCCCEEEEEEECCCCCCC
HRALPLVADAAEGIAELGAALKGWKAPAGWTDNAAKGKKAWQADAAKVTASTNAAFPSDA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHEEEECCCCCCCCCH
QVIGGVQRAMGSGVTLLHAAGGLPGELHKLWQAGTPGSYHAEYGFSTMGYEIAGGLGTKM
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCC
AKPGEEVVVMIGDGSYLMLNSEIATSVMLGLKLIIVLLDNRGYGCINRLQMATGGANFNN
CCCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCHHH
LLKDSRHEILPDIDFAAHAASMGAIAEKVPSIAGLENALQKAKTNDRTTVLVIDTDPLVS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
TDAGGHWWDVAVPEVSARPQVIAARKAYDEKRQMQSVGD
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ACRARPQGGIRRRGTRTAEETIMSKTIRLTMAQALTRFLSRQMTEIDGKKMPIFGGVWA
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEE
IFGHGNVAGMGEALYQVRDELPTFRAHNEQAMAHAAIAYAKANFRRRFMAATSSIGPGAL
EEECCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NMVTAAALAHVNRLPVLFLPGDVFANRIPDPVLQQAEDFSDGTATVNDCFRPVSRYFDRI
HHHHHHHHHHHHCCCEEEECCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
TRPEQIIPALSRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFAERVWHQRRPRPDR
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCH
HELAAAVTALKGAKKPLVIAGGGVLYSQASDALAKFVEGAGIPVCETQGGKSSLPDDHPL
HHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
NMAAVGVTGTSAANRLAEEADVVLAIGTRLQDFTTGSWALFKNSGKTIIGLNVQPFDAGK
CEEEEECCCCHHHHHHHHHCCEEEEECCCHHHCCCCCEEEEECCCCEEEEEEECCCCCCC
HRALPLVADAAEGIAELGAALKGWKAPAGWTDNAAKGKKAWQADAAKVTASTNAAFPSDA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHEEEECCCCCCCCCH
QVIGGVQRAMGSGVTLLHAAGGLPGELHKLWQAGTPGSYHAEYGFSTMGYEIAGGLGTKM
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCC
AKPGEEVVVMIGDGSYLMLNSEIATSVMLGLKLIIVLLDNRGYGCINRLQMATGGANFNN
CCCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCHHH
LLKDSRHEILPDIDFAAHAASMGAIAEKVPSIAGLENALQKAKTNDRTTVLVIDTDPLVS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
TDAGGHWWDVAVPEVSARPQVIAARKAYDEKRQMQSVGD
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA