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The map label for this gene is truA

Identifier: 13474062

GI number: 13474062

Start: 3856345

End: 3857091

Strand: Reverse

Name: truA

Synonym: mll4852

Alternate gene names: 13474062

Gene position: 3857091-3856345 (Counterclockwise)

Preceding gene: 13474063

Following gene: 13474059

Centisome position: 54.82

GC content: 68.41

Gene sequence:

>747_bases
ATGCCGCGTTTTCGCCTCGACATCGAATATGACGGCAGCCTGTTCGCCGGCTGGCAGCATCAGGCCGACCAGCCTTCGGT
GCAGCAGGCGATCGAGCAGGCGATCGAGAAATTCTGCGGCGAGGCGGTGCGGTTGCGCGCCGCCGGCCGCACCGATGCCG
GCGTGCATGCGAGCGCCCAGGTGGCGCATGTCGACCTCGCCAAGCCATGGCCCGGCGACAAGGTGCGCGATGCCGTCAAC
GCCCATCTGCAGGCGGCCGGCGCGCATGTCGCCATCCTGAAAGCGGCGATCGTCCCGGACGATTTCGACGCCCGCTTCTC
TGCCACCGGGCGCCACTATCTCTATCGCATCCTCAACCGGCGTGCGCCCGCGGCACTGGAAAAGGGCAAGGTGTGGTGGG
TGCCGAAGCGGCTCGATGCCGGCGCCATGCACGAGGCCGCGAAACTCCTGCTCGGCCGGCATGACTTCACCACCTTCCGT
TCGACCCAGTGCCAGGCCAACAGCCCGGTTCGGACGCTGGAACGCCTCGATGTTAGCCGGGCGGGCGACATGATCGAGGT
GAGGGCTTCGGCCCGTTCCTTCCTGCACAACCAGGTGCGCTCCATGGTCGGCTCGCTCAAGCGCGTCGGCGATGGCGGCT
GGACCGAGGCCGAGCTCAAGGCGGCGCTCGAAGCGCGCGACCGCGCCGCCTGCGGCCAGGTGGCGCCGCCCGACGGGCTT
TTTCTCATCGGCGTCGATTATCCCTGA

Upstream 100 bases:

>100_bases
AACGGTGACCTGCTCTACCTCTTTGTTTTAACGCAATTCCGGACGGAAAACCGTTTCACACTTTTCCTGGAATTGCTCAA
GGCGAGAGCTCTAGCCGCAA

Downstream 100 bases:

>100_bases
ATAGGGTCGGGGGGCTTCTGTTCAGAGCCCGGCAGGCTCTAACCCCGGGAACGGTCCGGAAAGGCTTTGAGCCCCGATAT
CGTCCGGCACGGAGATGCCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MPRFRLDIEYDGSLFAGWQHQADQPSVQQAIEQAIEKFCGEAVRLRAAGRTDAGVHASAQVAHVDLAKPWPGDKVRDAVN
AHLQAAGAHVAILKAAIVPDDFDARFSATGRHYLYRILNRRAPAALEKGKVWWVPKRLDAGAMHEAAKLLLGRHDFTTFR
STQCQANSPVRTLERLDVSRAGDMIEVRASARSFLHNQVRSMVGSLKRVGDGGWTEAELKAALEARDRAACGQVAPPDGL
FLIGVDYP

Sequences:

>Translated_248_residues
MPRFRLDIEYDGSLFAGWQHQADQPSVQQAIEQAIEKFCGEAVRLRAAGRTDAGVHASAQVAHVDLAKPWPGDKVRDAVN
AHLQAAGAHVAILKAAIVPDDFDARFSATGRHYLYRILNRRAPAALEKGKVWWVPKRLDAGAMHEAAKLLLGRHDFTTFR
STQCQANSPVRTLERLDVSRAGDMIEVRASARSFLHNQVRSMVGSLKRVGDGGWTEAELKAALEARDRAACGQVAPPDGL
FLIGVDYP
>Mature_247_residues
PRFRLDIEYDGSLFAGWQHQADQPSVQQAIEQAIEKFCGEAVRLRAAGRTDAGVHASAQVAHVDLAKPWPGDKVRDAVNA
HLQAAGAHVAILKAAIVPDDFDARFSATGRHYLYRILNRRAPAALEKGKVWWVPKRLDAGAMHEAAKLLLGRHDFTTFRS
TQCQANSPVRTLERLDVSRAGDMIEVRASARSFLHNQVRSMVGSLKRVGDGGWTEAELKAALEARDRAACGQVAPPDGLF
LIGVDYP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=36.8613138686131, Blast_Score=137, Evalue=9e-33,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=42.1052631578947, Blast_Score=184, Evalue=4e-48,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=28.0528052805281, Blast_Score=94, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_RHILO (Q98D55)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105630.1
- ProteinModelPortal:   Q98D55
- SMR:   Q98D55
- GeneID:   1228291
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4852
- NMPDR:   fig|266835.1.peg.3734
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27181; Mature: 27050

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFRLDIEYDGSLFAGWQHQADQPSVQQAIEQAIEKFCGEAVRLRAAGRTDAGVHASAQ
CCCEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCCCCCCE
VAHVDLAKPWPGDKVRDAVNAHLQAAGAHVAILKAAIVPDDFDARFSATGRHYLYRILNR
EEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHC
RAPAALEKGKVWWVPKRLDAGAMHEAAKLLLGRHDFTTFRSTQCQANSPVRTLERLDVSR
CCCCHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHH
AGDMIEVRASARSFLHNQVRSMVGSLKRVGDGGWTEAELKAALEARDRAACGQVAPPDGL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
FLIGVDYP
EEEEECCC
>Mature Secondary Structure 
PRFRLDIEYDGSLFAGWQHQADQPSVQQAIEQAIEKFCGEAVRLRAAGRTDAGVHASAQ
CCEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCCCCCCE
VAHVDLAKPWPGDKVRDAVNAHLQAAGAHVAILKAAIVPDDFDARFSATGRHYLYRILNR
EEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHC
RAPAALEKGKVWWVPKRLDAGAMHEAAKLLLGRHDFTTFRSTQCQANSPVRTLERLDVSR
CCCCHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHH
AGDMIEVRASARSFLHNQVRSMVGSLKRVGDGGWTEAELKAALEARDRAACGQVAPPDGL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
FLIGVDYP
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968