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The map label for this gene is ycdT [H]

Identifier: 13474053

GI number: 13474053

Start: 3848132

End: 3848893

Strand: Reverse

Name: ycdT [H]

Synonym: mll4839

Alternate gene names: 13474053

Gene position: 3848893-3848132 (Counterclockwise)

Preceding gene: 13474055

Following gene: 13474052

Centisome position: 54.7

GC content: 65.22

Gene sequence:

>762_bases
ATGAACAGCATCATTCTGAAAGCCGCCGCTATCGCTTTCGCTTCCGTCGCCGCCTCGCTTCTTCTGACGCTGATCATCGT
TCCGGCCCTGGGTTTCCCGATCAACCGAACGATCTGGCTGACCTCGACGCTCTGCCCGCTGGTGCTTGCATGGGTGGCCT
GTGCCAGCACCTTCTGGCAGAGCGACAGGCTGAAGAACGCGCACCGCGAACTGGCCCGGGCACACGCCCAGCTTGCCGCC
GCACATCGCCGCCTGTCGGAGAAAGCCAGCCGCGACGACATGACCGGCATGCTCAACCGGGAAAGTTTCTTTGCCGCGCT
GGACGGTTCCCGGCGCAAGTCCGACCGCGGCGCGCTGCTGATCATCGACGCCGATCATTTCAAGAAGATCAATGACAGTT
TCGGCCACCTGACCGGTGACGACGCACTGCTGCTGATCGCCAGCGCCATCGAACGCGGCGTGCGCAGCGGCGACGTGCTT
GGCCGCATCGGCGGCGAGGAGTTCGGCGCGTTCCTGGTCGGCGCCACCGAGCAGGAGGCCAAGCGTGTCGCCGAACGCAT
TCGCCGCGAAGTCGAACTGATCCGCTTCCGGCCGGTCGACGAACGGACCATTCCGCTGACCGTCAGCATCGGCGGCACCG
TCTGCGGCGAGGATGTCAACGTATCGGAACTGATGCGCGCCGCGGACCGGCGCCTCTACCAGGCCAAGCATCGGGGCCGC
AACCTGACGATCCTCGATACCGACATTTCCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
TTTAGTGGCAGAAATGGCCTCACGATTCAAATTTTAAGCAACCGGGAAAGGTGGCCTTCAGGGCTTGCTGGCACAGAGAC
AGTCCTGTGGGGAGCCTGCG

Downstream 100 bases:

>100_bases
GCCGGCCGGTCCGGCACACCCGCCCGTTAAGGAATTTCTAACCCTGTTTGCATTCGGTTGTACCGCCAGCCCGCCTTTCA
CCACCTCGAGGCCGGGCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNSIILKAAAIAFASVAASLLLTLIIVPALGFPINRTIWLTSTLCPLVLAWVACASTFWQSDRLKNAHRELARAHAQLAA
AHRRLSEKASRDDMTGMLNRESFFAALDGSRRKSDRGALLIIDADHFKKINDSFGHLTGDDALLLIASAIERGVRSGDVL
GRIGGEEFGAFLVGATEQEAKRVAERIRREVELIRFRPVDERTIPLTVSIGGTVCGEDVNVSELMRAADRRLYQAKHRGR
NLTILDTDISAAA

Sequences:

>Translated_253_residues
MNSIILKAAAIAFASVAASLLLTLIIVPALGFPINRTIWLTSTLCPLVLAWVACASTFWQSDRLKNAHRELARAHAQLAA
AHRRLSEKASRDDMTGMLNRESFFAALDGSRRKSDRGALLIIDADHFKKINDSFGHLTGDDALLLIASAIERGVRSGDVL
GRIGGEEFGAFLVGATEQEAKRVAERIRREVELIRFRPVDERTIPLTVSIGGTVCGEDVNVSELMRAADRRLYQAKHRGR
NLTILDTDISAAA
>Mature_253_residues
MNSIILKAAAIAFASVAASLLLTLIIVPALGFPINRTIWLTSTLCPLVLAWVACASTFWQSDRLKNAHRELARAHAQLAA
AHRRLSEKASRDDMTGMLNRESFFAALDGSRRKSDRGALLIIDADHFKKINDSFGHLTGDDALLLIASAIERGVRSGDVL
GRIGGEEFGAFLVGATEQEAKRVAERIRREVELIRFRPVDERTIPLTVSIGGTVCGEDVNVSELMRAADRRLYQAKHRGR
NLTILDTDISAAA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=40.4624277456647, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=34.5029239766082, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI145693134, Length=175, Percent_Identity=38.2857142857143, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=38.7878787878788, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=34.3373493975904, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=35.8024691358025, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=176, Percent_Identity=32.9545454545455, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=35.3658536585366, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=35.5932203389831, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=32, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=34.1772151898734, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=29.8136645962733, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787541, Length=163, Percent_Identity=30.6748466257669, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=119, Percent_Identity=31.0924369747899, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 27657; Mature: 27657

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIILKAAAIAFASVAASLLLTLIIVPALGFPINRTIWLTSTLCPLVLAWVACASTFWQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
SDRLKNAHRELARAHAQLAAAHRRLSEKASRDDMTGMLNRESFFAALDGSRRKSDRGALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHCCCCCCCCCCEEE
IIDADHFKKINDSFGHLTGDDALLLIASAIERGVRSGDVLGRIGGEEFGAFLVGATEQEA
EEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHCEEEECCCHHHH
KRVAERIRREVELIRFRPVDERTIPLTVSIGGTVCGEDVNVSELMRAADRRLYQAKHRGR
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHCCC
NLTILDTDISAAA
EEEEEECCCCCCC
>Mature Secondary Structure
MNSIILKAAAIAFASVAASLLLTLIIVPALGFPINRTIWLTSTLCPLVLAWVACASTFWQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
SDRLKNAHRELARAHAQLAAAHRRLSEKASRDDMTGMLNRESFFAALDGSRRKSDRGALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHCCCCCCCCCCEEE
IIDADHFKKINDSFGHLTGDDALLLIASAIERGVRSGDVLGRIGGEEFGAFLVGATEQEA
EEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHCEEEECCCHHHH
KRVAERIRREVELIRFRPVDERTIPLTVSIGGTVCGEDVNVSELMRAADRRLYQAKHRGR
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHCCC
NLTILDTDISAAA
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]