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The map label for this gene is gfo [H]

Identifier: 13474003

GI number: 13474003

Start: 3796845

End: 3797900

Strand: Direct

Name: gfo [H]

Synonym: mlr4777

Alternate gene names: 13474003

Gene position: 3796845-3797900 (Clockwise)

Preceding gene: 13474002

Following gene: 13474004

Centisome position: 53.96

GC content: 63.26

Gene sequence:

>1056_bases
ATGGCTCAACCTCTTCGCGTCGTCGTCGCCGGGCTCGGCAATATGGGCCGCAGCCATGCGCTGGCCTATCACACCAATCC
TGGCTTCGAGATCGCCGCCCTCATCAACCGCTCCGACGTGCCGCTGCCCGACGGGCTGTCCGGCTATGGCATCAGCCGGT
CCTTCGACGAGGCGCTGCGCGAGGAAAAGCCCGACGTCGCCTGCATCGCCACCTATTCCGACAGCCATGCCGACTATGCG
GTCAGGGCCTTCGAGGCCGGCTGCCACGTCTTTGTCGAAAAGCCGCTGGCAACGACGGTGGCCGACGCAAAACGCGTTGT
CGCGGCCGCCAAGGCCAACGGCAAAAAGCTGGTGATCGGCTATATCTTGCGCCACCACCCGTCCTGGATCCGGCTGATCG
CCGAGGCGCGCAAGCTCGGCGGCCCCTATGTCTTCCGCATGAACCTCAACCAGCAATCCTCCGGCCATACCTGGGAGACG
CACAAGCAATTGATGCAGACGACGTCGCCGATCGTCGACTGCGGCGTCCACTATCTCGACGTTATGCTGCAGATCACCGA
CGCCAGGCCGGTCGAGGTGCGCGGCATGGGCGTGCGGTTGACGCAGGAGGTTGCGCCCTCGATGTACAATTACGGCCACC
TGCAGGTGCTGTTCGATGACGGCTCGGTCGGCTGGTATGAAGCCGGCTGGGGTCCGATGATTTCCGAAACCGCCTTCTTC
GTGAAGGACGTCATTTCGCCGAATGGCTGCGTGTCGATTGTCATGAAGGAAGGTATCAAATCCGACGACATCGACACCCA
CACCAAGACCTCGACCATCCGCCTCCACAGTGCGGCGACCGGCCCGGATGGCAAGTTCGTCAAGGCGGACGAGATGCTGT
CCATGGCAGGCGAACCTGGCCACCAGGACCTCTGCGACCTCGAACAGGCCTTCGTGTTGAAGGCGGTCCGCGAGGACCTG
GATCTCACTCGCCACATGGACGACGCGGTCAAGTCGCTCGCCGTCTGCCTTGCCGCCGACGAGAGTGTGCGCAGCGGCGC
AGCCGTCAAACTCTAA

Upstream 100 bases:

>100_bases
CACTGTGAAAGTGAACAAGCAGCTTTGCGAGTTGCGGATGAGCGCGAGCCCCATGTGATGGTGGCTCTCAAGGCGCGCAG
CCAAGAAAAGGCGAACTGAA

Downstream 100 bases:

>100_bases
AGCTAGGAACAATATTGTGGGCTCGCTGAAGATCGAAAATGTGAAGAAGGCCTTCGGGCCGGTCGAGGTGCTGAAGGGCA
TCAACCTCGAAGTGACCGAT

Product: glucose-fructose oxidoreductase

Products: NA

Alternate protein names: GFOR [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MAQPLRVVVAGLGNMGRSHALAYHTNPGFEIAALINRSDVPLPDGLSGYGISRSFDEALREEKPDVACIATYSDSHADYA
VRAFEAGCHVFVEKPLATTVADAKRVVAAAKANGKKLVIGYILRHHPSWIRLIAEARKLGGPYVFRMNLNQQSSGHTWET
HKQLMQTTSPIVDCGVHYLDVMLQITDARPVEVRGMGVRLTQEVAPSMYNYGHLQVLFDDGSVGWYEAGWGPMISETAFF
VKDVISPNGCVSIVMKEGIKSDDIDTHTKTSTIRLHSAATGPDGKFVKADEMLSMAGEPGHQDLCDLEQAFVLKAVREDL
DLTRHMDDAVKSLAVCLAADESVRSGAAVKL

Sequences:

>Translated_351_residues
MAQPLRVVVAGLGNMGRSHALAYHTNPGFEIAALINRSDVPLPDGLSGYGISRSFDEALREEKPDVACIATYSDSHADYA
VRAFEAGCHVFVEKPLATTVADAKRVVAAAKANGKKLVIGYILRHHPSWIRLIAEARKLGGPYVFRMNLNQQSSGHTWET
HKQLMQTTSPIVDCGVHYLDVMLQITDARPVEVRGMGVRLTQEVAPSMYNYGHLQVLFDDGSVGWYEAGWGPMISETAFF
VKDVISPNGCVSIVMKEGIKSDDIDTHTKTSTIRLHSAATGPDGKFVKADEMLSMAGEPGHQDLCDLEQAFVLKAVREDL
DLTRHMDDAVKSLAVCLAADESVRSGAAVKL
>Mature_350_residues
AQPLRVVVAGLGNMGRSHALAYHTNPGFEIAALINRSDVPLPDGLSGYGISRSFDEALREEKPDVACIATYSDSHADYAV
RAFEAGCHVFVEKPLATTVADAKRVVAAAKANGKKLVIGYILRHHPSWIRLIAEARKLGGPYVFRMNLNQQSSGHTWETH
KQLMQTTSPIVDCGVHYLDVMLQITDARPVEVRGMGVRLTQEVAPSMYNYGHLQVLFDDGSVGWYEAGWGPMISETAFFV
KDVISPNGCVSIVMKEGIKSDDIDTHTKTSTIRLHSAATGPDGKFVKADEMLSMAGEPGHQDLCDLEQAFVLKAVREDLD
LTRHMDDAVKSLAVCLAADESVRSGAAVKL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=264, Percent_Identity=26.1363636363636, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008354
- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.99.28 [H]

Molecular weight: Translated: 38206; Mature: 38075

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPLRVVVAGLGNMGRSHALAYHTNPGFEIAALINRSDVPLPDGLSGYGISRSFDEALR
CCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
EEKPDVACIATYSDSHADYAVRAFEAGCHVFVEKPLATTVADAKRVVAAAKANGKKLVIG
HCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEE
YILRHHPSWIRLIAEARKLGGPYVFRMNLNQQSSGHTWETHKQLMQTTSPIVDCGVHYLD
HHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
VMLQITDARPVEVRGMGVRLTQEVAPSMYNYGHLQVLFDDGSVGWYEAGWGPMISETAFF
HHHEECCCCCEEEECCCEEEHHHHCHHHCCCCEEEEEEECCCCCEEECCCCCCHHHHHHH
VKDVISPNGCVSIVMKEGIKSDDIDTHTKTSTIRLHSAATGPDGKFVKADEMLSMAGEPG
HHHHCCCCCCEEHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHCCCCC
HQDLCDLEQAFVLKAVREDLDLTRHMDDAVKSLAVCLAADESVRSGAAVKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure 
AQPLRVVVAGLGNMGRSHALAYHTNPGFEIAALINRSDVPLPDGLSGYGISRSFDEALR
CCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
EEKPDVACIATYSDSHADYAVRAFEAGCHVFVEKPLATTVADAKRVVAAAKANGKKLVIG
HCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEE
YILRHHPSWIRLIAEARKLGGPYVFRMNLNQQSSGHTWETHKQLMQTTSPIVDCGVHYLD
HHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
VMLQITDARPVEVRGMGVRLTQEVAPSMYNYGHLQVLFDDGSVGWYEAGWGPMISETAFF
HHHEECCCCCEEEECCCEEEHHHHCHHHCCCCEEEEEEECCCCCEEECCCCCCHHHHHHH
VKDVISPNGCVSIVMKEGIKSDDIDTHTKTSTIRLHSAATGPDGKFVKADEMLSMAGEPG
HHHHCCCCCCEEHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHCCCCC
HQDLCDLEQAFVLKAVREDLDLTRHMDDAVKSLAVCLAADESVRSGAAVKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1537789; 8472911; 8994968 [H]