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The map label for this gene is gshB

Identifier: 13473969

GI number: 13473969

Start: 3756359

End: 3757300

Strand: Reverse

Name: gshB

Synonym: mll4735

Alternate gene names: 13473969

Gene position: 3757300-3756359 (Counterclockwise)

Preceding gene: 13473979

Following gene: 13473967

Centisome position: 53.4

GC content: 64.01

Gene sequence:

>942_bases
ATGAAACTGAAAATCGCCGTCCAGATGGACCACATCTCCACCGTGTCGATCGCCGGGGACACTTCGTTCGCGCTGTCGCT
GGAAGCGCAGCGGCGCGGCCATCAATTGTTCCACTACACGCCCGACCGGCTGTCGCTGCGCGACGGCAAGGTGTTTGCGC
GCATCGAGGAAATGCAGGTGCGCGACGAGAAGGGCAGCCACTATTCGCTGGGCGAGAAAGTGCGCACCGACCTGTCGGAG
ATGGACGTGGTCCTGCTGCGCCAGGATCCGCCCTTCGACATGAACTACATCACCACCACGCATATCCTCGAGCGCATCCA
CCCGAAGACCCTGGTGGTCAACGACCCGGCCTGGGTGCGCAACAGCCCGGAAAAGATCTTCGTCACCGAGTTTGCCGATT
TGATGCCGGACACGCTGATCACCAAGGATCCGCTGGAAGTCGCCGCCTTCCGCAAGGAGTTCGGCGACATCATCGTCAAG
CCGCTCTACGGCAATGGCGGCGCCGGCATCTTCCATCTGCACGAGGCCGACCGCAACCTCGCCTCGCTGCTCGAAATGTT
CGGCCAGCTGTTCCGCGAGCCCTATATCGTGCAGCGCTACCTCAAGGACGTGCGCAAGGGCGACAAGCGCATCATCCTAA
TCGACGGCGAGCCGGTCGGCGCCATCAACCGCGTGCCGGCCGAGCACGATTCCCGCTCCAACATGCATGTCGGCGGCCGC
GCCGAGAAGACCGAGCTGACGGAGCGCGAGCGCGAGATCTGCGCCCGCATCGGGCCGTCCCTCAGGGAACGCGGCTTCAT
CCTGGTCGGCATCGACGTCATCGGCGACTACATGACCGAGATCAACGTCACCTCGCCGACCGGCGTGCGCGAGGTGCAGC
GTTTTGGCGGCGCCGACATCGCCAGCCTGTTCTGGGACGCGGTCGAGGGGAAGCGGAAGTAG

Upstream 100 bases:

>100_bases
TGGGGGTTGTGTCCTGCTGTTGCCGATCTTCAGACGTCGCGATTGGCAGAAGGCGCGGCGACCCTTATATCGCTCTCGTC
GACTGCGCTGGGGACCCGCC

Downstream 100 bases:

>100_bases
CGTTTTTCGGTTCGTATTAACCATGTTCCGCCTTTTCAACCGGGCTCAATCCGGCTGCAACCAGAGCGATTTCCTTGTTC
TCGTAATGTTCTTGATTTGA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKLKIAVQMDHISTVSIAGDTSFALSLEAQRRGHQLFHYTPDRLSLRDGKVFARIEEMQVRDEKGSHYSLGEKVRTDLSE
MDVVLLRQDPPFDMNYITTTHILERIHPKTLVVNDPAWVRNSPEKIFVTEFADLMPDTLITKDPLEVAAFRKEFGDIIVK
PLYGNGGAGIFHLHEADRNLASLLEMFGQLFREPYIVQRYLKDVRKGDKRIILIDGEPVGAINRVPAEHDSRSNMHVGGR
AEKTELTEREREICARIGPSLRERGFILVGIDVIGDYMTEINVTSPTGVREVQRFGGADIASLFWDAVEGKRK

Sequences:

>Translated_313_residues
MKLKIAVQMDHISTVSIAGDTSFALSLEAQRRGHQLFHYTPDRLSLRDGKVFARIEEMQVRDEKGSHYSLGEKVRTDLSE
MDVVLLRQDPPFDMNYITTTHILERIHPKTLVVNDPAWVRNSPEKIFVTEFADLMPDTLITKDPLEVAAFRKEFGDIIVK
PLYGNGGAGIFHLHEADRNLASLLEMFGQLFREPYIVQRYLKDVRKGDKRIILIDGEPVGAINRVPAEHDSRSNMHVGGR
AEKTELTEREREICARIGPSLRERGFILVGIDVIGDYMTEINVTSPTGVREVQRFGGADIASLFWDAVEGKRK
>Mature_313_residues
MKLKIAVQMDHISTVSIAGDTSFALSLEAQRRGHQLFHYTPDRLSLRDGKVFARIEEMQVRDEKGSHYSLGEKVRTDLSE
MDVVLLRQDPPFDMNYITTTHILERIHPKTLVVNDPAWVRNSPEKIFVTEFADLMPDTLITKDPLEVAAFRKEFGDIIVK
PLYGNGGAGIFHLHEADRNLASLLEMFGQLFREPYIVQRYLKDVRKGDKRIILIDGEPVGAINRVPAEHDSRSNMHVGGR
AEKTELTEREREICARIGPSLRERGFILVGIDVIGDYMTEINVTSPTGVREVQRFGGADIASLFWDAVEGKRK

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=311, Percent_Identity=40.8360128617363, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1787076, Length=173, Percent_Identity=30.635838150289, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_RHILO (Q98DE8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105537.1
- ProteinModelPortal:   Q98DE8
- SMR:   Q98DE8
- GeneID:   1228198
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4735
- NMPDR:   fig|266835.1.peg.3641
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- ProtClustDB:   PRK05246
- BRENDA:   6.3.2.3
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 35483; Mature: 35483

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKIAVQMDHISTVSIAGDTSFALSLEAQRRGHQLFHYTPDRLSLRDGKVFARIEEMQV
CEEEEEEEECCCEEEEEECCCEEEEEEEHHHCCCEEEEECCCCCEECCCHHEEEHHHHHH
RDEKGSHYSLGEKVRTDLSEMDVVLLRQDPPFDMNYITTTHILERIHPKTLVVNDPAWVR
HCCCCCCCCCHHHHHHCHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHC
NSPEKIFVTEFADLMPDTLITKDPLEVAAFRKEFGDIIVKPLYGNGGAGIFHLHEADRNL
CCCCEEEEEHHHHHCCCHHCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEHHCCHH
ASLLEMFGQLFREPYIVQRYLKDVRKGDKRIILIDGEPVGAINRVPAEHDSRSNMHVGGR
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCC
AEKTELTEREREICARIGPSLRERGFILVGIDVIGDYMTEINVTSPTGVREVQRFGGADI
CHHHHHHHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHCCCHH
ASLFWDAVEGKRK
HHHHHHHHCCCCC
>Mature Secondary Structure
MKLKIAVQMDHISTVSIAGDTSFALSLEAQRRGHQLFHYTPDRLSLRDGKVFARIEEMQV
CEEEEEEEECCCEEEEEECCCEEEEEEEHHHCCCEEEEECCCCCEECCCHHEEEHHHHHH
RDEKGSHYSLGEKVRTDLSEMDVVLLRQDPPFDMNYITTTHILERIHPKTLVVNDPAWVR
HCCCCCCCCCHHHHHHCHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHC
NSPEKIFVTEFADLMPDTLITKDPLEVAAFRKEFGDIIVKPLYGNGGAGIFHLHEADRNL
CCCCEEEEEHHHHHCCCHHCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEHHCCHH
ASLLEMFGQLFREPYIVQRYLKDVRKGDKRIILIDGEPVGAINRVPAEHDSRSNMHVGGR
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCC
AEKTELTEREREICARIGPSLRERGFILVGIDVIGDYMTEINVTSPTGVREVQRFGGADI
CHHHHHHHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHCCCHH
ASLFWDAVEGKRK
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968