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The map label for this gene is glcB

Identifier: 13473913

GI number: 13473913

Start: 3698567

End: 3700732

Strand: Direct

Name: glcB

Synonym: mlr4664

Alternate gene names: 13473913

Gene position: 3698567-3700732 (Clockwise)

Preceding gene: 13473912

Following gene: 13473914

Centisome position: 52.57

GC content: 65.6

Gene sequence:

>2166_bases
ATGACCGATCGCATCGAGATCGCCGGATTGCGGATTGCCGGCGAGCTGCATGATTTTGTGGCTGGCGAGGCCTTGCCCGG
CACCGGCATCGCCGCCGATGCGTTCTGGAGCGGTTTTTCGGCCATCGTCCACGATCTCGCGCCGAAGAACCGGGCGCTGC
TCAAAAAACGTGACGCCATGCAGGAAAGGCTCGACGGCTGGTATCGCGACAATGGCGCGCCGGTCGACATGGAGGTCTAC
AAGAGCTTCCTGAAAGAGATCGGCTATCTCGTTCCCGAAGGGCCGGCCTTCAGCGTGTCGACGGACAATGTCGATCCCGA
AATCGCCGTCGTTGCCGGGCCGCAGCTCGTCGTGCCGGTGATGAATGCGCGCTATGCGCTCAATGCCGCCAATGCGCGCT
GGGGCTCGCTCTACGACGCCCTCTATGGCACCGATGCCATTCCCGAGACCGGCGGCGCGGAAAAGGGCAAGGGCTTCAAC
CCGGCGCGCGGCGCCAAGGTCATCGCCTGGGCCAAGGATTTTCTCGACCAGTCGGTGCCGCTCACCTCGGGCAAATGGGC
TGGGGTGAACGGCCTGTCAGTGGCGCATGGCGCGCTGAAGCTTGGCGCCGGCGCCGGCGGCACCACGCTGGCCGACCCCA
GGCAATTCGCCGGCTATCGCGGCGATGCCGCCAACCCCGATGCCGTGCTGCTGGTGAAGAACGGGTTGCACATCGAGATC
GTCGTCGACCGCAACAACCAGATCGGCCGCACCGACCCCGCCGGCATTGCCGACGTCATCCTGGAATCGGCGCTGACCAC
CATCCAGGACTGCGAGGATTCGGTCGCGGCGGTGGATGCGCAGGACAAGGTCGTCGTCTACCGCAACTGGCTCGGCCTGA
TGAAAGGCGACCTCGCCGAAGAGATCTCCAAGGCTGGCCGGAGTTTCGTCCGCAAGCTCAACGCCGACCGCGCCTACACG
GCGCCCGCCGGCGGCCAGATCACCGTTCCCGGCCGCTCGCTGATGCTGGTCAGGAATGTCGGCCACCTGATGACCAATCC
GGCAATCCTGGACCGCGACGGCAACGAAGTGCCGGAAGGCATCATGGATGCCGCGCTGACGGCGCTGATCGCGCTGCACG
ATGTCGGGCCGGAGGGCCGCCGCGCCAATTCCCGCGCCGGCTCGATGTATGTGGTGAAGCCCAAGATGCACGGGCCGGAG
GAAGTCGCCTTCGCCGTCGAGATCTTCGACCGCGTCGAGGCGCTGCTCGGGATGCCCAGGAATACCATCAAGATGGGCAT
CATGGACGAGGAGCGGCGCACCACCGTCAACCTCAAGGAAGCGATCCGTGCCGCGCGCGAGCGCGTGGTGTTCATCAACA
CCGGCTTCCTCGACCGCACCGGCGACGAGATCCACACCTCGATGGAAGCCGGCCCGATGATCCGCAAGGGCGACATGAAG
CAGGCCGCCTGGATTTCCGCCTATGAGGCGTGGAATGTCGACACCGGGCTTGAATGCGGGCTGGCCGGCCACGCCCAGAT
CGGCAAGGGCATGTGGGCGATGCCGGATCTGATGGCGGCGATGCTGGAACAGAAGATCGCCCACCCCAAGGCCGGCGCCA
ACACCGCCTGGGTGCCGTCGCCGACGGCGGCGACGCTGCACGCCACGCACTATCACAAGGTCGACGTGCATGCCGTGCAG
GCGGCGCTGAAGAGCCGGCCGAAGGCGAAACTCGACGACATCTTGTCGGTGCCGGTCGCCGTGCGGCCGAACTGGACGCC
CGACGAGATCCAACGCGAACTCGACAACAACGCGCAAGGCATTCTCGGCTATGTCGTGCGCTGGATCGACCAGGGCGTCG
GCTGCTCGAAAGTGCCCGACATCAACGATGTCGGCCTGATGGAAGACCGCGCCACGCTGCGCATCTCCTCGCAGCACATC
GCCAACTGGCTTCGCCACAAAGTGTGTTCGGAGATCCAGGTGCGCGATTCCCTGCAGCGCATGGCGGCAATCGTCGACCG
CCAGAATGTCGGCGATCCGCTCTACCGGCCGATGGCGCCGGATTTCGACAAATCCATCGCCTTCCAGGCCGCCTGCGACC
TGGTGTTCAAGGGAACCAGCCAGCCCAACGGCTATACGGAGCCGGTGCTGCACGCGCGGCGGCTGGAGCTGAAGGCGCAG
AGATAG

Upstream 100 bases:

>100_bases
CGAACGAGGCCAAAATGCCCGACCTTCACCCTAAGCTGAATTGACCCTGCAACCTCAACATGCCAAACGCCCCGCAAAGC
TTTTCATCTGGAGTAGCCTG

Downstream 100 bases:

>100_bases
GAGATCGGGTAGCCAGCTAAGCATCTGCCGCGTCGAACCGCAGAAAGGAGAATTTCCTTAACGATGCGAAATAGCTGTTG
ATAGTTGATCGCTGACAAAT

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTDRIEIAGLRIAGELHDFVAGEALPGTGIAADAFWSGFSAIVHDLAPKNRALLKKRDAMQERLDGWYRDNGAPVDMEVY
KSFLKEIGYLVPEGPAFSVSTDNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPETGGAEKGKGFN
PARGAKVIAWAKDFLDQSVPLTSGKWAGVNGLSVAHGALKLGAGAGGTTLADPRQFAGYRGDAANPDAVLLVKNGLHIEI
VVDRNNQIGRTDPAGIADVILESALTTIQDCEDSVAAVDAQDKVVVYRNWLGLMKGDLAEEISKAGRSFVRKLNADRAYT
APAGGQITVPGRSLMLVRNVGHLMTNPAILDRDGNEVPEGIMDAALTALIALHDVGPEGRRANSRAGSMYVVKPKMHGPE
EVAFAVEIFDRVEALLGMPRNTIKMGIMDEERRTTVNLKEAIRAARERVVFINTGFLDRTGDEIHTSMEAGPMIRKGDMK
QAAWISAYEAWNVDTGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVHAVQ
AALKSRPKAKLDDILSVPVAVRPNWTPDEIQRELDNNAQGILGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQHI
ANWLRHKVCSEIQVRDSLQRMAAIVDRQNVGDPLYRPMAPDFDKSIAFQAACDLVFKGTSQPNGYTEPVLHARRLELKAQ
R

Sequences:

>Translated_721_residues
MTDRIEIAGLRIAGELHDFVAGEALPGTGIAADAFWSGFSAIVHDLAPKNRALLKKRDAMQERLDGWYRDNGAPVDMEVY
KSFLKEIGYLVPEGPAFSVSTDNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPETGGAEKGKGFN
PARGAKVIAWAKDFLDQSVPLTSGKWAGVNGLSVAHGALKLGAGAGGTTLADPRQFAGYRGDAANPDAVLLVKNGLHIEI
VVDRNNQIGRTDPAGIADVILESALTTIQDCEDSVAAVDAQDKVVVYRNWLGLMKGDLAEEISKAGRSFVRKLNADRAYT
APAGGQITVPGRSLMLVRNVGHLMTNPAILDRDGNEVPEGIMDAALTALIALHDVGPEGRRANSRAGSMYVVKPKMHGPE
EVAFAVEIFDRVEALLGMPRNTIKMGIMDEERRTTVNLKEAIRAARERVVFINTGFLDRTGDEIHTSMEAGPMIRKGDMK
QAAWISAYEAWNVDTGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVHAVQ
AALKSRPKAKLDDILSVPVAVRPNWTPDEIQRELDNNAQGILGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQHI
ANWLRHKVCSEIQVRDSLQRMAAIVDRQNVGDPLYRPMAPDFDKSIAFQAACDLVFKGTSQPNGYTEPVLHARRLELKAQ
R
>Mature_720_residues
TDRIEIAGLRIAGELHDFVAGEALPGTGIAADAFWSGFSAIVHDLAPKNRALLKKRDAMQERLDGWYRDNGAPVDMEVYK
SFLKEIGYLVPEGPAFSVSTDNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPETGGAEKGKGFNP
ARGAKVIAWAKDFLDQSVPLTSGKWAGVNGLSVAHGALKLGAGAGGTTLADPRQFAGYRGDAANPDAVLLVKNGLHIEIV
VDRNNQIGRTDPAGIADVILESALTTIQDCEDSVAAVDAQDKVVVYRNWLGLMKGDLAEEISKAGRSFVRKLNADRAYTA
PAGGQITVPGRSLMLVRNVGHLMTNPAILDRDGNEVPEGIMDAALTALIALHDVGPEGRRANSRAGSMYVVKPKMHGPEE
VAFAVEIFDRVEALLGMPRNTIKMGIMDEERRTTVNLKEAIRAARERVVFINTGFLDRTGDEIHTSMEAGPMIRKGDMKQ
AAWISAYEAWNVDTGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVHAVQA
ALKSRPKAKLDDILSVPVAVRPNWTPDEIQRELDNNAQGILGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQHIA
NWLRHKVCSEIQVRDSLQRMAAIVDRQNVGDPLYRPMAPDFDKSIAFQAACDLVFKGTSQPNGYTEPVLHARRLELKAQR

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily

Homologues:

Organism=Escherichia coli, GI1789348, Length=725, Percent_Identity=60.551724137931, Blast_Score=894, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MASZ_RHILO (Q98DK4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105481.1
- ProteinModelPortal:   Q98DK4
- SMR:   Q98DK4
- GeneID:   1228142
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4664
- NMPDR:   fig|266835.1.peg.3585
- HOGENOM:   HBG350005
- OMA:   PKMHGPD
- ProtClustDB:   PRK02999
- BRENDA:   2.3.3.9
- GO:   GO:0005737
- HAMAP:   MF_00641
- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253
- PANTHER:   PTHR21631:SF1
- TIGRFAMs:   TIGR01345

Pfam domain/function: PF01274 Malate_synthase; SSF51645 Malat_synth_like

EC number: =2.3.3.9

Molecular weight: Translated: 78060; Mature: 77929

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: ACT_SITE 338-338 ACT_SITE 629-629

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRIEIAGLRIAGELHDFVAGEALPGTGIAADAFWSGFSAIVHDLAPKNRALLKKRDAM
CCCCEEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QERLDGWYRDNGAPVDMEVYKSFLKEIGYLVPEGPAFSVSTDNVDPEIAVVAGPQLVVPV
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECCEEEEEE
MNARYALNAANARWGSLYDALYGTDAIPETGGAEKGKGFNPARGAKVIAWAKDFLDQSVP
CCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCC
LTSGKWAGVNGLSVAHGALKLGAGAGGTTLADPRQFAGYRGDAANPDAVLLVKNGLHIEI
CCCCCCCCCCCCHHHHHHHEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCEEEE
VVDRNNQIGRTDPAGIADVILESALTTIQDCEDSVAAVDAQDKVVVYRNWLGLMKGDLAE
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHH
EISKAGRSFVRKLNADRAYTAPAGGQITVPGRSLMLVRNVGHLMTNPAILDRDGNEVPEG
HHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCEEEEHHHHHHHCCCEEECCCCCCCHHH
IMDAALTALIALHDVGPEGRRANSRAGSMYVVKPKMHGPEEVAFAVEIFDRVEALLGMPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
NTIKMGIMDEERRTTVNLKEAIRAARERVVFINTGFLDRTGDEIHTSMEAGPMIRKGDMK
HHEEECCCCCCHHHEECHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCH
QAAWISAYEAWNVDTGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPS
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PTAATLHATHYHKVDVHAVQAALKSRPKAKLDDILSVPVAVRPNWTPDEIQRELDNNAQG
CCCEEEEECEEEEHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHHHHHHHHCCCHHH
ILGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQHIANWLRHKVCSEIQVRDSLQR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
MAAIVDRQNVGDPLYRPMAPDFDKSIAFQAACDLVFKGTSQPNGYTEPVLHARRLELKAQ
HHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHCCC
R
C
>Mature Secondary Structure 
TDRIEIAGLRIAGELHDFVAGEALPGTGIAADAFWSGFSAIVHDLAPKNRALLKKRDAM
CCCEEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QERLDGWYRDNGAPVDMEVYKSFLKEIGYLVPEGPAFSVSTDNVDPEIAVVAGPQLVVPV
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECCEEEEEE
MNARYALNAANARWGSLYDALYGTDAIPETGGAEKGKGFNPARGAKVIAWAKDFLDQSVP
CCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCC
LTSGKWAGVNGLSVAHGALKLGAGAGGTTLADPRQFAGYRGDAANPDAVLLVKNGLHIEI
CCCCCCCCCCCCHHHHHHHEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCEEEE
VVDRNNQIGRTDPAGIADVILESALTTIQDCEDSVAAVDAQDKVVVYRNWLGLMKGDLAE
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHH
EISKAGRSFVRKLNADRAYTAPAGGQITVPGRSLMLVRNVGHLMTNPAILDRDGNEVPEG
HHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCEEEEHHHHHHHCCCEEECCCCCCCHHH
IMDAALTALIALHDVGPEGRRANSRAGSMYVVKPKMHGPEEVAFAVEIFDRVEALLGMPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
NTIKMGIMDEERRTTVNLKEAIRAARERVVFINTGFLDRTGDEIHTSMEAGPMIRKGDMK
HHEEECCCCCCHHHEECHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCH
QAAWISAYEAWNVDTGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPS
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PTAATLHATHYHKVDVHAVQAALKSRPKAKLDDILSVPVAVRPNWTPDEIQRELDNNAQG
CCCEEEEECEEEEHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHHHHHHHHCCCHHH
ILGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQHIANWLRHKVCSEIQVRDSLQR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
MAAIVDRQNVGDPLYRPMAPDFDKSIAFQAACDLVFKGTSQPNGYTEPVLHARRLELKAQ
HHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968