Click here to switch to the map view.

The map label for this gene is mepA [H]

Identifier: 13473893

GI number: 13473893

Start: 3676619

End: 3677806

Strand: Direct

Name: mepA [H]

Synonym: mlr4639

Alternate gene names: 13473893

Gene position: 3676619-3677806 (Clockwise)

Preceding gene: 13473892

Following gene: 13473894

Centisome position: 52.25

GC content: 67.34

Gene sequence:

>1188_bases
ATGCGGTGGGGATCCGGTTTCATGAAGACAGCGATGCGGCCATCATGTTGTTTTGGCGCAATTCCCAAGGGAAAGCGCTA
CGCGCTTTTCCCGGGAAAACCGCTTCACACTTTTCCTGGAATTGCTTTGGGCAAGAAATCACTGCTGGCAGCAGTGCTCG
GTTTGCTTGGCCTCGCCGCCCAGCCGGCGCTCTCCGAGGAACGGGCAAAAGACCTGTTCGGCGCTGAGAAACTGCCGACC
GCGACCGCCCCGCAATCCTTCGGCTTCTATTCCAAGGGCTGCTTCGCCGGCGGTGTCGCCATCCCCATGGACGGCCCGAC
CTGGCAGGTCATGCGGCCCTCGCGCAACCGCCGCTGGGGCCACCCGGCGATGATCGCGCTGATCGAGAAACTGTCGCGCG
ACGCCGACGCCGACGGCTGGCCTGGCCTGTTGATCGGCGACATTTCGCAGCCGCGCGGCGGCCCGATGATGACAGGCCAT
GCCTCGCACCAGATCGGGCTCGATGCCGACATCTGGCTGACGCCGATGCCTGACCGCACCATGACCAGGGCGCAGCGCGA
ATCGATGAGCGCCACCTTGATGGTCGACGAGAAGACACATCTGGTGAAGGACGCGCTGTGGACGCCGCAGCACACGGCGC
TCTTGAAGCGGGCGGCGAGCTACCCCGAGGTGGAACGCATCCTGGTCAATCCCGGCATCAAGAAAAAGCTCTGCGACACC
GTCAAGGGCGATCGTAGCTGGCTGCGCAAGATCCGGCCCTTCTGGGGCCACGACTATCATTTCCACATGCGCATCGGCTG
CCAGCGGGGCTCGCCCGACTGCAAGGGGCAGGAGGCGACGCCGGATGATGACGGCTGCGGCAAGCCGCTGGCCTGGTGGT
TCACGCAGGAGCCCTGGCGGCCCAACAAGAACCCCGACGCGCCGAAGGCCCGCGACCTGATGACGATGGCCAACCTGCCC
AAGGAATGCCGCGCCGTGCTCGACGCGCCGGGCCCGGCCTCGGCGGAGGCGGCGACCTATCACGGCGGCGCCGTGCCGGT
CGCGGTCGCGGCGCCCGAGCCGGAAGCGTCGCCGCAGCCCGCCACCACGGCCAGCGGTGGCGGCGATTTGCCGAGCGCGG
CAAATGCATTCGCCGCGACCCCCAGAATGGGCGCGCCGGTGCCACGGCCACGACCGCCCGCGAAGTGA

Upstream 100 bases:

>100_bases
TGGCGCGACGGCGAGGCGCGCAGCCCGATGAAACGCTGATTGCGCGAAGGAGTAGGGCAATCGAAGCAGGCTGCGTTGCG
TGCTATGATTCGGCCTTGGG

Downstream 100 bases:

>100_bases
TTGCCGCTGCGCCACCGGTCGGCTCAATCGGTTTAGCGAGCTGTTTTCGGGAAATAGTGGCCGTTTGGCCGCAGAAGCTC
TCTCGGCGCGCGGAGGGGTT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRWGSGFMKTAMRPSCCFGAIPKGKRYALFPGKPLHTFPGIALGKKSLLAAVLGLLGLAAQPALSEERAKDLFGAEKLPT
ATAPQSFGFYSKGCFAGGVAIPMDGPTWQVMRPSRNRRWGHPAMIALIEKLSRDADADGWPGLLIGDISQPRGGPMMTGH
ASHQIGLDADIWLTPMPDRTMTRAQRESMSATLMVDEKTHLVKDALWTPQHTALLKRAASYPEVERILVNPGIKKKLCDT
VKGDRSWLRKIRPFWGHDYHFHMRIGCQRGSPDCKGQEATPDDDGCGKPLAWWFTQEPWRPNKNPDAPKARDLMTMANLP
KECRAVLDAPGPASAEAATYHGGAVPVAVAAPEPEASPQPATTASGGGDLPSAANAFAATPRMGAPVPRPRPPAK

Sequences:

>Translated_395_residues
MRWGSGFMKTAMRPSCCFGAIPKGKRYALFPGKPLHTFPGIALGKKSLLAAVLGLLGLAAQPALSEERAKDLFGAEKLPT
ATAPQSFGFYSKGCFAGGVAIPMDGPTWQVMRPSRNRRWGHPAMIALIEKLSRDADADGWPGLLIGDISQPRGGPMMTGH
ASHQIGLDADIWLTPMPDRTMTRAQRESMSATLMVDEKTHLVKDALWTPQHTALLKRAASYPEVERILVNPGIKKKLCDT
VKGDRSWLRKIRPFWGHDYHFHMRIGCQRGSPDCKGQEATPDDDGCGKPLAWWFTQEPWRPNKNPDAPKARDLMTMANLP
KECRAVLDAPGPASAEAATYHGGAVPVAVAAPEPEASPQPATTASGGGDLPSAANAFAATPRMGAPVPRPRPPAK
>Mature_395_residues
MRWGSGFMKTAMRPSCCFGAIPKGKRYALFPGKPLHTFPGIALGKKSLLAAVLGLLGLAAQPALSEERAKDLFGAEKLPT
ATAPQSFGFYSKGCFAGGVAIPMDGPTWQVMRPSRNRRWGHPAMIALIEKLSRDADADGWPGLLIGDISQPRGGPMMTGH
ASHQIGLDADIWLTPMPDRTMTRAQRESMSATLMVDEKTHLVKDALWTPQHTALLKRAASYPEVERILVNPGIKKKLCDT
VKGDRSWLRKIRPFWGHDYHFHMRIGCQRGSPDCKGQEATPDDDGCGKPLAWWFTQEPWRPNKNPDAPKARDLMTMANLP
KECRAVLDAPGPASAEAATYHGGAVPVAVAAPEPEASPQPATTASGGGDLPSAANAFAATPRMGAPVPRPRPPAK

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=250, Percent_Identity=39.6, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 42600; Mature: 42600

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWGSGFMKTAMRPSCCFGAIPKGKRYALFPGKPLHTFPGIALGKKSLLAAVLGLLGLAA
CCCCCHHHHHHCCCCHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
QPALSEERAKDLFGAEKLPTATAPQSFGFYSKGCFAGGVAIPMDGPTWQVMRPSRNRRWG
CCCHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCC
HPAMIALIEKLSRDADADGWPGLLIGDISQPRGGPMMTGHASHQIGLDADIWLTPMPDRT
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCEEEECCCCHH
MTRAQRESMSATLMVDEKTHLVKDALWTPQHTALLKRAASYPEVERILVNPGIKKKLCDT
HHHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHEEECCCHHHHHHHH
VKGDRSWLRKIRPFWGHDYHFHMRIGCQRGSPDCKGQEATPDDDGCGKPLAWWFTQEPWR
HCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PNKNPDAPKARDLMTMANLPKECRAVLDAPGPASAEAATYHGGAVPVAVAAPEPEASPQP
CCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCC
ATTASGGGDLPSAANAFAATPRMGAPVPRPRPPAK
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRWGSGFMKTAMRPSCCFGAIPKGKRYALFPGKPLHTFPGIALGKKSLLAAVLGLLGLAA
CCCCCHHHHHHCCCCHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
QPALSEERAKDLFGAEKLPTATAPQSFGFYSKGCFAGGVAIPMDGPTWQVMRPSRNRRWG
CCCHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCC
HPAMIALIEKLSRDADADGWPGLLIGDISQPRGGPMMTGHASHQIGLDADIWLTPMPDRT
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCEEEECCCCHH
MTRAQRESMSATLMVDEKTHLVKDALWTPQHTALLKRAASYPEVERILVNPGIKKKLCDT
HHHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHEEECCCHHHHHHHH
VKGDRSWLRKIRPFWGHDYHFHMRIGCQRGSPDCKGQEATPDDDGCGKPLAWWFTQEPWR
HCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PNKNPDAPKARDLMTMANLPKECRAVLDAPGPASAEAATYHGGAVPVAVAAPEPEASPQP
CCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCC
ATTASGGGDLPSAANAFAATPRMGAPVPRPRPPAK
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]