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The map label for this gene is cysK [H]

Identifier: 13473875

GI number: 13473875

Start: 3662780

End: 3663760

Strand: Reverse

Name: cysK [H]

Synonym: mll4616

Alternate gene names: 13473875

Gene position: 3663760-3662780 (Counterclockwise)

Preceding gene: 13473876

Following gene: 13473874

Centisome position: 52.07

GC content: 65.85

Gene sequence:

>981_bases
ATGAACAAGCCCGTCACCTCCGCCCGCGTCCCCGGCCGCGGCCGCATCTACAGTTCCATCACCGACACGATCGGCGACAC
GCCCCTGGTCCGGCTCGACAAATTCGCCAAGGAGAAGGGGGTTGTCGCCAATCTCGTGGCCAAGCTCGAATTCTTCAACC
CGATCGCCTCGGTCAAGGACCGCATCGGCGTGGCGATGATCGAGGCGCTGGAGGCGGCCGGCAAGATCTCGCCCGGCAAG
ACGACGCTGATCGAACCGACCTCGGGCAACACCGGCATCGCGCTTGCCTTCGCCGCCGCCGCCAAGGGCTACAAGCTCAT
CCTGACCATGCCCGAAACGATGTCGGTCGAGCGCCGCAAGATGCTGGCGCTGCTCGGCGCCGAACTGGTGCTGACCGAAG
GGCCGAAAGGCATGAAGGGCGCCATCGCCAAGGCCGACGAGCTGGCCGCGACAATCCCCAATGCCGTTATCCCGCAGCAG
TTCGAAAACCCCGCCAACCCGGAAATCCACCGCAAGACGACAGCGCAGGAGATCTGGAACGACACGCAGGGCGAGGTCGA
CATCTTCGTCGCCGGCATCGGCACGGGCGGCACCATCACCGGCGTCGGCCAGGTGCTGAAGAAGCTCAAGCCCTCGCTGC
ATGTCGTCGCCGTCGAGCCGGAAGCCTCGCCGGTGCTGTCGGGCGGCCAGCCCGGCCCGCACAAGATCCAGGGCATCGGC
GCCGGCTTCGCACCAAAAATCCTCGACACCACGATCTATGACGAGATCATCAAGGTTTCCAACGAGGATTCCGTCGCCAA
TGCCCGCCTCGTCGCCCGTCTCGAAGGGGTGCCGGTCGGCATCTCGTCGGGTGCGGCGCTGCAGGCGGCAATCGTCGTCG
GCTCGCGGCCGGAGAACAAGGGCAAGACCCTGGTCGTGGTCATCCCCGACTTCGCCGAGCGCTACCTGTCGACGATCTTG
TTCGAGGGGCTGGGGGCTTAG

Upstream 100 bases:

>100_bases
CCCACTCGGATATTTCCGGCCGACAGCTTATGTTGCGCGGCTGAAAGCCCTATCTTGCCGGCTGCAATGCCACTTGCCTG
AAATCCTGGGAGCGAAACCA

Downstream 100 bases:

>100_bases
GCGTTCGTACCTCGAAGATTGGCCTCAGCGGCCCCTCCCGGTCGTCATCCCAGGGCGAAGCAGGAGCGAAGCTCCGTCGC
GTAGAACCCTGGGATCCATG

Product: cysteine synthase, cytosolic O-acetylserine(thiol)lyase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNKPVTSARVPGRGRIYSSITDTIGDTPLVRLDKFAKEKGVVANLVAKLEFFNPIASVKDRIGVAMIEALEAAGKISPGK
TTLIEPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLALLGAELVLTEGPKGMKGAIAKADELAATIPNAVIPQQ
FENPANPEIHRKTTAQEIWNDTQGEVDIFVAGIGTGGTITGVGQVLKKLKPSLHVVAVEPEASPVLSGGQPGPHKIQGIG
AGFAPKILDTTIYDEIIKVSNEDSVANARLVARLEGVPVGISSGAALQAAIVVGSRPENKGKTLVVVIPDFAERYLSTIL
FEGLGA

Sequences:

>Translated_326_residues
MNKPVTSARVPGRGRIYSSITDTIGDTPLVRLDKFAKEKGVVANLVAKLEFFNPIASVKDRIGVAMIEALEAAGKISPGK
TTLIEPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLALLGAELVLTEGPKGMKGAIAKADELAATIPNAVIPQQ
FENPANPEIHRKTTAQEIWNDTQGEVDIFVAGIGTGGTITGVGQVLKKLKPSLHVVAVEPEASPVLSGGQPGPHKIQGIG
AGFAPKILDTTIYDEIIKVSNEDSVANARLVARLEGVPVGISSGAALQAAIVVGSRPENKGKTLVVVIPDFAERYLSTIL
FEGLGA
>Mature_326_residues
MNKPVTSARVPGRGRIYSSITDTIGDTPLVRLDKFAKEKGVVANLVAKLEFFNPIASVKDRIGVAMIEALEAAGKISPGK
TTLIEPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLALLGAELVLTEGPKGMKGAIAKADELAATIPNAVIPQQ
FENPANPEIHRKTTAQEIWNDTQGEVDIFVAGIGTGGTITGVGQVLKKLKPSLHVVAVEPEASPVLSGGQPGPHKIQGIG
AGFAPKILDTTIYDEIIKVSNEDSVANARLVARLEGVPVGISSGAALQAAIVVGSRPENKGKTLVVVIPDFAERYLSTIL
FEGLGA

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=317, Percent_Identity=40.6940063091483, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI295821200, Length=317, Percent_Identity=40.6940063091483, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4557415, Length=317, Percent_Identity=40.6940063091483, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=53.9184952978056, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=39.4736842105263, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=52.4752475247525, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=50, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI115535073, Length=309, Percent_Identity=51.7799352750809, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=50.5084745762712, Blast_Score=290, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17561720, Length=303, Percent_Identity=48.1848184818482, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=48.5849056603774, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=324, Percent_Identity=40.7407407407407, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=38.2262996941896, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=44.6280991735537, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=321, Percent_Identity=38.3177570093458, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321449, Length=332, Percent_Identity=31.9277108433735, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24643623, Length=323, Percent_Identity=37.7708978328173, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI20129101, Length=323, Percent_Identity=37.7708978328173, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34057; Mature: 34057

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPVTSARVPGRGRIYSSITDTIGDTPLVRLDKFAKEKGVVANLVAKLEFFNPIASVKD
CCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHH
RIGVAMIEALEAAGKISPGKTTLIEPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRK
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHH
MLALLGAELVLTEGPKGMKGAIAKADELAATIPNAVIPQQFENPANPEIHRKTTAQEIWN
HHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHC
DTQGEVDIFVAGIGTGGTITGVGQVLKKLKPSLHVVAVEPEASPVLSGGQPGPHKIQGIG
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECC
AGFAPKILDTTIYDEIIKVSNEDSVANARLVARLEGVPVGISSGAALQAAIVVGSRPENK
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCCCCCCCCEEEEEEECCCCCCC
GKTLVVVIPDFAERYLSTILFEGLGA
CCEEEEECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKPVTSARVPGRGRIYSSITDTIGDTPLVRLDKFAKEKGVVANLVAKLEFFNPIASVKD
CCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHH
RIGVAMIEALEAAGKISPGKTTLIEPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRK
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHH
MLALLGAELVLTEGPKGMKGAIAKADELAATIPNAVIPQQFENPANPEIHRKTTAQEIWN
HHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHC
DTQGEVDIFVAGIGTGGTITGVGQVLKKLKPSLHVVAVEPEASPVLSGGQPGPHKIQGIG
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECC
AGFAPKILDTTIYDEIIKVSNEDSVANARLVARLEGVPVGISSGAALQAAIVVGSRPENK
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCCCCCCCCEEEEEEECCCCCCC
GKTLVVVIPDFAERYLSTILFEGLGA
CCEEEEECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]