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The map label for this gene is priA [H]

Identifier: 13473463

GI number: 13473463

Start: 3251672

End: 3253855

Strand: Reverse

Name: priA [H]

Synonym: mll4071

Alternate gene names: 13473463

Gene position: 3253855-3251672 (Counterclockwise)

Preceding gene: 13473465

Following gene: 13473462

Centisome position: 46.25

GC content: 67.77

Gene sequence:

>2184_bases
ATGATCGAAGATTCGCCCTTTGTCGCAGCCGCGCCGGTTCTGGTGCCGATGCCTGCCGAACGCCCTTACACCTATGCCGT
GCCGGCCGGCATGCGCGTCGTGCCGGGGTCAATCGTGCGCGTGCCGCTCGGGCCGCGCCAGGTCGCCGGGATCGTCTGGG
ACGGCGCGGTCGAGACTGTCGATGCGAAAAAGCTGCGCTCGATCGAACAGGTTTTCGACTGCCCGCCGATCGACCGTTCG
ATGCGCCGTTTCGTCGACTGGGTGGCGCAATACACGCTGTCGGCGCCCGGCATGGTGGCGCGCATGCTGCTCAGAGCGCC
GGAGGCGTTCGACCCGGAACCCTGGATCGAGGGGCTGCAGCGCACGCTTATCATTCCCGACCGGATGACGGATGCGCGGG
CGCGTGTGCTGGAGACGGCCGAAGGTGGGTTGGCCTGGACGCGATCGGGCCTTGCCCATGCGGCCGGTGTGTCGTCGACG
GTGATCGAGGGGCTGAAGGCGCAAGGCGTGTTCGAGACGGTGATGATCCCGCCGCGCCCGGTGGTGGCAGCACCTGACCC
GAGCTATGCGGTGCCCGAGCTGATGCCGGACCAGAGCGACGCCGCATCGATGCTGCGCGCCAATGTCGCGGCCGGCGCCT
TCAATGTCGCGCTGCTCGATGGCGTCACCGGGTCCGGCAAGACGGAGGTGTATTTCGAGGCGGTGGCGGCCGCGCTCGAC
CAGGGCAAGCAGGTGCTGATCCTTTTGCCGGAAATCGCGTTGACCCACGCTTTCCTCGAACGCTTCCAGCAGCGCTTCGG
CGCCAAGCCGGGCGAATGGCATTCCGACCTGCCGCCCAGGATGCGCGAAAAAGTCTGGCGGCAAGTGGCTGAGGGCACGG
TGCGCGTCGTTGCCGGTGCGCGCTCGGCGCTGTTCCTGCCGTTCAAGGAGCTTGGCCTGATCGTCGTCGATGAGGAGCAC
GACCCCGCCTACAAGCAGGAGGACCGTGTCTTCTACAATGCGCGCGACATGGCGGTGGTGCGCGGCCATATCGGTGGCTT
CCCGGTGGTGCTGGCGTCGGCGACGCCATCGGTCGAAAGCCGGGTCAATGCGAGCCAAGGCAAGTACAACAGGGCCATCC
TTTCCGCCCGCTTCGCCGAGGCGGCGCTGCCGCAGCTGAAGTCGATCGACATGCGACGCGCGCCACCGGCGCGTGGTGGC
TTCCTGTCGCCCGTGCTGATCGACCATATGCGCAAGACGCTGGAGAAGAAGGAACAGTCGCTCCTGTTCCTCAACCGGCG
CGGCTATGCGCCGCTGACGCTGTGCCGCGTCTGCGGCCACCGTTTCGGCTGCCCGGTCTGTTCGGCCTGGCTGGTCGAGC
ATCGCTTTCGCGGCCAGCTGGTCTGCCATCATTGCGGGCACAATGAGCGCCGCCCCGAAGCCTGCCCTGAATGCGGCACG
CTCGATCATCTGGTCGCCTGCGGCCCGGGCGTCGAGCGCATCGCCGAGGAGGTCGTCACGCATTTCCCCGATGCGCGCAC
CATCGTCTTGTCATCCGACCTGATGGGCGGGGTGCGGCGGCTGCGGCTGGAACTGGAGGCGATCGCCGATGGCGAAGCCG
ATATCGTCATCGGCACGCAGCTCGTCGCCAAGGGCCACAATTTTCCCAACATGACGCTGGTCGGCGTCGTCGATGCCGAT
CTCGGCCTCGCCAATGGCGATCCGCGGGCCGCCGAGCGCACCTTCCAACTGCTCAGCCAGGTGACCGGCCGCGCAGGCCG
CACCGGCAAGAAGAGCCTCGGCCTGCTGCAGACGTTCCAGCCCGACCATCCGGTGATGCGGGCGATCGTCTCGGGCGATG
CCGAGGCTTTTTACGAACGAGAGATCGCCGAGCGCGAACGGGCGGCCCTACCGCCGTTCGGCCGGTTGGCCGGCGTCATC
GTAAGCGCGGTGACGCGGGCGGAAGCGGAAGGTCATGCACGGGGCCTGCGCCGCGCGGCGCCGGAGGCCTCGGACCTGTT
CGTGCTGGGTCCGGCCGAGGCGCCGCTGTCCCTGCTCGGCGGCCGCCATCGCTTCCGCCTGCTGATCCAGGGCGAACGGC
GCGCAGACATGCAAGGGTTTATCCGCGCCATGCTGGCCAATGGGCCGAAGCAGCGCGGTTCGGTCCGGGTGCAGGTCGAT
ATCGACCCGCAGAGCTTTTTGTAA

Upstream 100 bases:

>100_bases
AAAATTTCATGGCGGGTCTCCTGAAGATTCTTATGTGCATGCCATGCACATTCGGGGTCGGCCTTTGATCTAGACCAAAG
TCAGGGCAAGGTCACGCCTC

Downstream 100 bases:

>100_bases
GGTCGCGATATCGGCGATACGCTGCTGGCCAGCGAGAAATGGAAGATCACATGAAAACCCTTGGCCTTCTGGGCGGCATG
AGCTGGGAGTCGACCGCCAT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 727; Mature: 727

Protein sequence:

>727_residues
MIEDSPFVAAAPVLVPMPAERPYTYAVPAGMRVVPGSIVRVPLGPRQVAGIVWDGAVETVDAKKLRSIEQVFDCPPIDRS
MRRFVDWVAQYTLSAPGMVARMLLRAPEAFDPEPWIEGLQRTLIIPDRMTDARARVLETAEGGLAWTRSGLAHAAGVSST
VIEGLKAQGVFETVMIPPRPVVAAPDPSYAVPELMPDQSDAASMLRANVAAGAFNVALLDGVTGSGKTEVYFEAVAAALD
QGKQVLILLPEIALTHAFLERFQQRFGAKPGEWHSDLPPRMREKVWRQVAEGTVRVVAGARSALFLPFKELGLIVVDEEH
DPAYKQEDRVFYNARDMAVVRGHIGGFPVVLASATPSVESRVNASQGKYNRAILSARFAEAALPQLKSIDMRRAPPARGG
FLSPVLIDHMRKTLEKKEQSLLFLNRRGYAPLTLCRVCGHRFGCPVCSAWLVEHRFRGQLVCHHCGHNERRPEACPECGT
LDHLVACGPGVERIAEEVVTHFPDARTIVLSSDLMGGVRRLRLELEAIADGEADIVIGTQLVAKGHNFPNMTLVGVVDAD
LGLANGDPRAAERTFQLLSQVTGRAGRTGKKSLGLLQTFQPDHPVMRAIVSGDAEAFYEREIAERERAALPPFGRLAGVI
VSAVTRAEAEGHARGLRRAAPEASDLFVLGPAEAPLSLLGGRHRFRLLIQGERRADMQGFIRAMLANGPKQRGSVRVQVD
IDPQSFL

Sequences:

>Translated_727_residues
MIEDSPFVAAAPVLVPMPAERPYTYAVPAGMRVVPGSIVRVPLGPRQVAGIVWDGAVETVDAKKLRSIEQVFDCPPIDRS
MRRFVDWVAQYTLSAPGMVARMLLRAPEAFDPEPWIEGLQRTLIIPDRMTDARARVLETAEGGLAWTRSGLAHAAGVSST
VIEGLKAQGVFETVMIPPRPVVAAPDPSYAVPELMPDQSDAASMLRANVAAGAFNVALLDGVTGSGKTEVYFEAVAAALD
QGKQVLILLPEIALTHAFLERFQQRFGAKPGEWHSDLPPRMREKVWRQVAEGTVRVVAGARSALFLPFKELGLIVVDEEH
DPAYKQEDRVFYNARDMAVVRGHIGGFPVVLASATPSVESRVNASQGKYNRAILSARFAEAALPQLKSIDMRRAPPARGG
FLSPVLIDHMRKTLEKKEQSLLFLNRRGYAPLTLCRVCGHRFGCPVCSAWLVEHRFRGQLVCHHCGHNERRPEACPECGT
LDHLVACGPGVERIAEEVVTHFPDARTIVLSSDLMGGVRRLRLELEAIADGEADIVIGTQLVAKGHNFPNMTLVGVVDAD
LGLANGDPRAAERTFQLLSQVTGRAGRTGKKSLGLLQTFQPDHPVMRAIVSGDAEAFYEREIAERERAALPPFGRLAGVI
VSAVTRAEAEGHARGLRRAAPEASDLFVLGPAEAPLSLLGGRHRFRLLIQGERRADMQGFIRAMLANGPKQRGSVRVQVD
IDPQSFL
>Mature_727_residues
MIEDSPFVAAAPVLVPMPAERPYTYAVPAGMRVVPGSIVRVPLGPRQVAGIVWDGAVETVDAKKLRSIEQVFDCPPIDRS
MRRFVDWVAQYTLSAPGMVARMLLRAPEAFDPEPWIEGLQRTLIIPDRMTDARARVLETAEGGLAWTRSGLAHAAGVSST
VIEGLKAQGVFETVMIPPRPVVAAPDPSYAVPELMPDQSDAASMLRANVAAGAFNVALLDGVTGSGKTEVYFEAVAAALD
QGKQVLILLPEIALTHAFLERFQQRFGAKPGEWHSDLPPRMREKVWRQVAEGTVRVVAGARSALFLPFKELGLIVVDEEH
DPAYKQEDRVFYNARDMAVVRGHIGGFPVVLASATPSVESRVNASQGKYNRAILSARFAEAALPQLKSIDMRRAPPARGG
FLSPVLIDHMRKTLEKKEQSLLFLNRRGYAPLTLCRVCGHRFGCPVCSAWLVEHRFRGQLVCHHCGHNERRPEACPECGT
LDHLVACGPGVERIAEEVVTHFPDARTIVLSSDLMGGVRRLRLELEAIADGEADIVIGTQLVAKGHNFPNMTLVGVVDAD
LGLANGDPRAAERTFQLLSQVTGRAGRTGKKSLGLLQTFQPDHPVMRAIVSGDAEAFYEREIAERERAALPPFGRLAGVI
VSAVTRAEAEGHARGLRRAAPEASDLFVLGPAEAPLSLLGGRHRFRLLIQGERRADMQGFIRAMLANGPKQRGSVRVQVD
IDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=737, Percent_Identity=35.5495251017639, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1787357, Length=145, Percent_Identity=31.0344827586207, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 79163; Mature: 79163

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEDSPFVAAAPVLVPMPAERPYTYAVPAGMRVVPGSIVRVPLGPRQVAGIVWDGAVETV
CCCCCCCEEECCEEEECCCCCCEEEECCCCCEECCCCEEEECCCHHHHHEEEECCCHHHH
DAKKLRSIEQVFDCPPIDRSMRRFVDWVAQYTLSAPGMVARMLLRAPEAFDPEPWIEGLQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHH
RTLIIPDRMTDARARVLETAEGGLAWTRSGLAHAAGVSSTVIEGLKAQGVFETVMIPPRP
HEEECCCCCCHHHHHHHHHCCCCCHHHHCCHHHHCCCHHHHHHHHHCCCCEEEEECCCCC
VVAAPDPSYAVPELMPDQSDAASMLRANVAAGAFNVALLDGVTGSGKTEVYFEAVAAALD
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
QGKQVLILLPEIALTHAFLERFQQRFGAKPGEWHSDLPPRMREKVWRQVAEGTVRVVAGA
CCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECC
RSALFLPFKELGLIVVDEEHDPAYKQEDRVFYNARDMAVVRGHIGGFPVVLASATPSVES
CCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCEEEEECCCCCHHH
RVNASQGKYNRAILSARFAEAALPQLKSIDMRRAPPARGGFLSPVLIDHMRKTLEKKEQS
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLFLNRRGYAPLTLCRVCGHRFGCPVCSAWLVEHRFRGQLVCHHCGHNERRPEACPECGT
EEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
LDHLVACGPGVERIAEEVVTHFPDARTIVLSSDLMGGVRRLRLELEAIADGEADIVIGTQ
HHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
LVAKGHNFPNMTLVGVVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGKKSLGLLQTFQ
HHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHCC
PDHPVMRAIVSGDAEAFYEREIAERERAALPPFGRLAGVIVSAVTRAEAEGHARGLRRAA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEASDLFVLGPAEAPLSLLGGRHRFRLLIQGERRADMQGFIRAMLANGPKQRGSVRVQVD
CCCCCEEEECCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHCCCEEEEEE
IDPQSFL
CCHHHCC
>Mature Secondary Structure
MIEDSPFVAAAPVLVPMPAERPYTYAVPAGMRVVPGSIVRVPLGPRQVAGIVWDGAVETV
CCCCCCCEEECCEEEECCCCCCEEEECCCCCEECCCCEEEECCCHHHHHEEEECCCHHHH
DAKKLRSIEQVFDCPPIDRSMRRFVDWVAQYTLSAPGMVARMLLRAPEAFDPEPWIEGLQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHH
RTLIIPDRMTDARARVLETAEGGLAWTRSGLAHAAGVSSTVIEGLKAQGVFETVMIPPRP
HEEECCCCCCHHHHHHHHHCCCCCHHHHCCHHHHCCCHHHHHHHHHCCCCEEEEECCCCC
VVAAPDPSYAVPELMPDQSDAASMLRANVAAGAFNVALLDGVTGSGKTEVYFEAVAAALD
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
QGKQVLILLPEIALTHAFLERFQQRFGAKPGEWHSDLPPRMREKVWRQVAEGTVRVVAGA
CCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECC
RSALFLPFKELGLIVVDEEHDPAYKQEDRVFYNARDMAVVRGHIGGFPVVLASATPSVES
CCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCEEEEECCCCCHHH
RVNASQGKYNRAILSARFAEAALPQLKSIDMRRAPPARGGFLSPVLIDHMRKTLEKKEQS
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLFLNRRGYAPLTLCRVCGHRFGCPVCSAWLVEHRFRGQLVCHHCGHNERRPEACPECGT
EEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
LDHLVACGPGVERIAEEVVTHFPDARTIVLSSDLMGGVRRLRLELEAIADGEADIVIGTQ
HHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
LVAKGHNFPNMTLVGVVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGKKSLGLLQTFQ
HHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHCC
PDHPVMRAIVSGDAEAFYEREIAERERAALPPFGRLAGVIVSAVTRAEAEGHARGLRRAA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEASDLFVLGPAEAPLSLLGGRHRFRLLIQGERRADMQGFIRAMLANGPKQRGSVRVQVD
CCCCCEEEECCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHCCCEEEEEE
IDPQSFL
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]