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The map label for this gene is livJ [C]

Identifier: 13473388

GI number: 13473388

Start: 3175194

End: 3176309

Strand: Reverse

Name: livJ [C]

Synonym: mll3970

Alternate gene names: 13473388

Gene position: 3176309-3175194 (Counterclockwise)

Preceding gene: 13473389

Following gene: 13473387

Centisome position: 45.14

GC content: 63.53

Gene sequence:

>1116_bases
ATGAAAAAGACACTTTTGTCCGCCGTCGCCCTGACCGCGCTGGTCGCGTTCGGCGGCAACGCGTGGGCTGATGTAATATT
CGGCGTTGCCGGTCCGATCACCGGTCCGAACGCGGCCTTCGGCGCACAGCTGCAGAAGGGCGCAGAGGCGGCGGTCGCCG
AGATCAATGCCAAGGGCGGCATCAACGGCGAGCAGATCAAGCTCGAAGTCGGCGACGACGTCTCCGATCCGAAGCAAGGC
ATCTCGGTCGCCAACAAGTTCGTCGGCGACGGCGTCAAGTTCGTGATCGGCCACTTCAACTCGGGCGTCTCGATCCCGGC
CTCGGAAGTCTACGCCGAAAACAACATCGTCGAAATCACCCCGGCCGCGACCAACCCGAAGTTCACCGAGCGCGGCCTGT
GGAACGTGTTCCGCACCTGCGGACGCGACGATCAGCAGGGCAGCATCGCCGGCGCCTACATCGCCGCGAACTTCAAGGAT
GCCAAGGTCGCTGTCGTGCACGACAAGACCCCTTATGGCCAGGGCCTCGCCGATGAAACCAAGAAGGCGATGAACGCCGC
CGGCGTCAAGGAAGTGATGTATGAAGGCGTCAATGTCGGCGACAAGGACTTCTCGGCGCTCATCGCCAAGATGAAGGAAG
CCGGCGTCACCCTGATCTATTGGGGCGGCCTGCACACCGAAGCCGGCCTGATCATCCGTCAGTCGGCGGACCAGGGCCTC
AAGGCACCGATGATGTCGGGTGACGGCATCGTGACGGACGAACTCGCCGCGATCGCGGGCGACGCTGTCGCCGGCACGCT
CAACACGTTCGGACCCGATCCGCGCCTGATCCCGGCCAACAAGGAACTCGTCGAGAAGTTCCGTGCGCAGGGCTTCGAGC
CGGAAGCCTATACGCTCTACGCCTATGCCGCCGTGCAGGTGGTCGCCGAGGCGGCTGCCGCCGCCAAGACGAACGACCCG
CAGGCCGTTGCCAAGGCGATGCATGAAAGCGGCCCGTTCCCGAGCGTGCTGGGCGATCTCGCCTATGACGCCAAGGGCGA
CCCGAAGCTGCCGGGCTACATCATGTACGAGTGGAAGAAGAAGGACGACGGCAAGTATTCCTGGTTCCCGAAATAA

Upstream 100 bases:

>100_bases
TGACAAGTGCCTGAAAATCGGCAAACTATGCTTTCTGCGATAAGGGTGGGCATCGCATGAAAGCTTCATCCACGCCCACT
CCGTTAATGGGAGCGTTTAA

Downstream 100 bases:

>100_bases
GCTGCTCTGGCAGGTCTGAAATCCAAGATGCCCGGCGCAACGCGCCGGGCATTTTCTTTGGCGCATTCCGGTGGCCGATG
AGCCTCTTTGTGGGCTCCTC

Product: periplasmic binding protein of ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKKTLLSAVALTALVAFGGNAWADVIFGVAGPITGPNAAFGAQLQKGAEAAVAEINAKGGINGEQIKLEVGDDVSDPKQG
ISVANKFVGDGVKFVIGHFNSGVSIPASEVYAENNIVEITPAATNPKFTERGLWNVFRTCGRDDQQGSIAGAYIAANFKD
AKVAVVHDKTPYGQGLADETKKAMNAAGVKEVMYEGVNVGDKDFSALIAKMKEAGVTLIYWGGLHTEAGLIIRQSADQGL
KAPMMSGDGIVTDELAAIAGDAVAGTLNTFGPDPRLIPANKELVEKFRAQGFEPEAYTLYAYAAVQVVAEAAAAAKTNDP
QAVAKAMHESGPFPSVLGDLAYDAKGDPKLPGYIMYEWKKKDDGKYSWFPK

Sequences:

>Translated_371_residues
MKKTLLSAVALTALVAFGGNAWADVIFGVAGPITGPNAAFGAQLQKGAEAAVAEINAKGGINGEQIKLEVGDDVSDPKQG
ISVANKFVGDGVKFVIGHFNSGVSIPASEVYAENNIVEITPAATNPKFTERGLWNVFRTCGRDDQQGSIAGAYIAANFKD
AKVAVVHDKTPYGQGLADETKKAMNAAGVKEVMYEGVNVGDKDFSALIAKMKEAGVTLIYWGGLHTEAGLIIRQSADQGL
KAPMMSGDGIVTDELAAIAGDAVAGTLNTFGPDPRLIPANKELVEKFRAQGFEPEAYTLYAYAAVQVVAEAAAAAKTNDP
QAVAKAMHESGPFPSVLGDLAYDAKGDPKLPGYIMYEWKKKDDGKYSWFPK
>Mature_371_residues
MKKTLLSAVALTALVAFGGNAWADVIFGVAGPITGPNAAFGAQLQKGAEAAVAEINAKGGINGEQIKLEVGDDVSDPKQG
ISVANKFVGDGVKFVIGHFNSGVSIPASEVYAENNIVEITPAATNPKFTERGLWNVFRTCGRDDQQGSIAGAYIAANFKD
AKVAVVHDKTPYGQGLADETKKAMNAAGVKEVMYEGVNVGDKDFSALIAKMKEAGVTLIYWGGLHTEAGLIIRQSADQGL
KAPMMSGDGIVTDELAAIAGDAVAGTLNTFGPDPRLIPANKELVEKFRAQGFEPEAYTLYAYAAVQVVAEAAAAAKTNDP
QAVAKAMHESGPFPSVLGDLAYDAKGDPKLPGYIMYEWKKKDDGKYSWFPK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=346, Percent_Identity=39.5953757225434, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789867, Length=360, Percent_Identity=38.6111111111111, Blast_Score=248, Evalue=4e-67,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 38992; Mature: 38992

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLLSAVALTALVAFGGNAWADVIFGVAGPITGPNAAFGAQLQKGAEAAVAEINAKGG
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHEECCCCC
INGEQIKLEVGDDVSDPKQGISVANKFVGDGVKFVIGHFNSGVSIPASEVYAENNIVEIT
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCCCEEEEE
PAATNPKFTERGLWNVFRTCGRDDQQGSIAGAYIAANFKDAKVAVVHDKTPYGQGLADET
ECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCHHHH
KKAMNAAGVKEVMYEGVNVGDKDFSALIAKMKEAGVTLIYWGGLHTEAGLIIRQSADQGL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCC
KAPMMSGDGIVTDELAAIAGDAVAGTLNTFGPDPRLIPANKELVEKFRAQGFEPEAYTLY
CCCCCCCCCCCHHHHHHHHCCHHHCCHHCCCCCCCEECCCHHHHHHHHHCCCCCCCEEEH
AYAAVQVVAEAAAAAKTNDPQAVAKAMHESGPFPSVLGDLAYDAKGDPKLPGYIMYEWKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEE
KDDGKYSWFPK
CCCCCCCCCCC
>Mature Secondary Structure
MKKTLLSAVALTALVAFGGNAWADVIFGVAGPITGPNAAFGAQLQKGAEAAVAEINAKGG
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHEECCCCC
INGEQIKLEVGDDVSDPKQGISVANKFVGDGVKFVIGHFNSGVSIPASEVYAENNIVEIT
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCCCEEEEE
PAATNPKFTERGLWNVFRTCGRDDQQGSIAGAYIAANFKDAKVAVVHDKTPYGQGLADET
ECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCHHHH
KKAMNAAGVKEVMYEGVNVGDKDFSALIAKMKEAGVTLIYWGGLHTEAGLIIRQSADQGL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCC
KAPMMSGDGIVTDELAAIAGDAVAGTLNTFGPDPRLIPANKELVEKFRAQGFEPEAYTLY
CCCCCCCCCCCHHHHHHHHCCHHHCCHHCCCCCCCEECCCHHHHHHHHHCCCCCCCEEEH
AYAAVQVVAEAAAAAKTNDPQAVAKAMHESGPFPSVLGDLAYDAKGDPKLPGYIMYEWKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEE
KDDGKYSWFPK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA