Click here to switch to the map view.

The map label for this gene is tolB

Identifier: 13473330

GI number: 13473330

Start: 3125395

End: 3126705

Strand: Reverse

Name: tolB

Synonym: mll3888

Alternate gene names: 13473330

Gene position: 3126705-3125395 (Counterclockwise)

Preceding gene: 13473331

Following gene: 13473329

Centisome position: 44.44

GC content: 64.0

Gene sequence:

>1311_bases
ATGAGATCCTTCCTCAAGCCGCTCCTGACGATTGCAGCCATGGCGCTGGGCATGACGGCCGTAATCCCCATGCCGGCATG
GGCGCTCGTCGAGCTCAACGTCAACAAGGGCAATGTCGAGCCGCTGCCAATCGCCATCACGGATTTCCAGGGCGGCGATG
CGCTTGGCGCGCAGATTTCCCAGATCGTCACGGCTGACCTGAAACGCTCCGGCCTGTTCGCGCCGATCGACAAGAGCGCC
TTCATCGAGAAGATCTCGAACCCGGATGCCGCCCCCCGCTTCGACGACTGGAAGGTGATTAACGCGCAGGCGCTGGTCAC
CGGCAGCGTCAGCAAGGAAGCTGACGGCCGCATCCGTGCGCAGTACCGGCTTTGGGATACTTTTGCCGGCCAGCAGATGT
CGGGTGAGCAGTTCTTCGCCAACGACGCCAACCAGCGCCGCGTCGCCCACATCATCGCCGACGCCATCTATGAGCGGCTG
ACCGGTGAGAAGGGCTATTTCGACACGCGCGTGGTGTTCATCGACGAGTCCGGCGCCAAGAATGCGCGCAAGAAGCGCCT
GGCTATCATGGACCAGGATGGCGCCAACGTCCGCTATCTCTCGGACGGACGCTCGATCGTGCTGACGCCGCGTTTCTCGC
CGAACCGGCAGGAAATCACCTACATGTCCTATGAGAGCGGCCAGCCGCGGGTCTATCTCCTGCAGATCGAGACCGGGCAG
CGCGAACTGGTCGGCAATTTCCCCGGCATGACCTTTGCACCGCGCTTCTCGCCCGACGGCCAGAAGGTGATCATGAGCCT
GCTGCGCGACGACGGCAATTCCAACATCTTCGCCATGGACCTGCGCAGCCGCTCGACGACGCGGCTGACCAACTCGACCG
CCATCGACACCTCGCCCTCCTATTCGCCCGACGGCAGCAAGGTGGTGTTCACCTCCGACCGCGGCGGTCGCGCGCAGATC
TATGTCATGGGCGCCGACGGCTCGGGCCAGACCCGCATCTCCTTCGGCGACGGCGTCTATTCGACGCCGGTGTGGTCGCC
GCGCGGCGACCTCATCGCCTTCACCAAGCAGACCGGCGGCGAATTCCAGATCGGTGTCATGAAGACCGACGGTTCCGGCG
AGCGCATCCTGTCGTCCGGTTTCCAGCAGGAAGGACCAACCTGGGCGCCGAACGGCCGCGTGCTGATGTTCTTCCGCGAC
TCCAATGGCGGGCCGAAACTGGTTTCGGTCGACCTAACCGGCCGCAACGAGCAGCCGATCCCGACGGCGAACTTCGCGTC
GGATCCCGCTTGGTCGCCCTTGTTGGAATAG

Upstream 100 bases:

>100_bases
GGACATCTGGAACGACATCCGGGTCACTTTCGATCCAAGGGAGATGCTTGGCCTCTAGGCCTGGCGCCCACCCAAATCAA
AAGAAGAGAAGCGAGAAGAT

Downstream 100 bases:

>100_bases
CGTCGGCCGAATTGCCACACATGCTGAAAAGGGGCCGTTTCGGCCCCTTTTCCACGCCTTCGTCGCGTTTTGGCCGCATT
TCCGCCTAGGGTCCGCGCCA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRSFLKPLLTIAAMALGMTAVIPMPAWALVELNVNKGNVEPLPIAITDFQGGDALGAQISQIVTADLKRSGLFAPIDKSA
FIEKISNPDAAPRFDDWKVINAQALVTGSVSKEADGRIRAQYRLWDTFAGQQMSGEQFFANDANQRRVAHIIADAIYERL
TGEKGYFDTRVVFIDESGAKNARKKRLAIMDQDGANVRYLSDGRSIVLTPRFSPNRQEITYMSYESGQPRVYLLQIETGQ
RELVGNFPGMTFAPRFSPDGQKVIMSLLRDDGNSNIFAMDLRSRSTTRLTNSTAIDTSPSYSPDGSKVVFTSDRGGRAQI
YVMGADGSGQTRISFGDGVYSTPVWSPRGDLIAFTKQTGGEFQIGVMKTDGSGERILSSGFQQEGPTWAPNGRVLMFFRD
SNGGPKLVSVDLTGRNEQPIPTANFASDPAWSPLLE

Sequences:

>Translated_436_residues
MRSFLKPLLTIAAMALGMTAVIPMPAWALVELNVNKGNVEPLPIAITDFQGGDALGAQISQIVTADLKRSGLFAPIDKSA
FIEKISNPDAAPRFDDWKVINAQALVTGSVSKEADGRIRAQYRLWDTFAGQQMSGEQFFANDANQRRVAHIIADAIYERL
TGEKGYFDTRVVFIDESGAKNARKKRLAIMDQDGANVRYLSDGRSIVLTPRFSPNRQEITYMSYESGQPRVYLLQIETGQ
RELVGNFPGMTFAPRFSPDGQKVIMSLLRDDGNSNIFAMDLRSRSTTRLTNSTAIDTSPSYSPDGSKVVFTSDRGGRAQI
YVMGADGSGQTRISFGDGVYSTPVWSPRGDLIAFTKQTGGEFQIGVMKTDGSGERILSSGFQQEGPTWAPNGRVLMFFRD
SNGGPKLVSVDLTGRNEQPIPTANFASDPAWSPLLE
>Mature_436_residues
MRSFLKPLLTIAAMALGMTAVIPMPAWALVELNVNKGNVEPLPIAITDFQGGDALGAQISQIVTADLKRSGLFAPIDKSA
FIEKISNPDAAPRFDDWKVINAQALVTGSVSKEADGRIRAQYRLWDTFAGQQMSGEQFFANDANQRRVAHIIADAIYERL
TGEKGYFDTRVVFIDESGAKNARKKRLAIMDQDGANVRYLSDGRSIVLTPRFSPNRQEITYMSYESGQPRVYLLQIETGQ
RELVGNFPGMTFAPRFSPDGQKVIMSLLRDDGNSNIFAMDLRSRSTTRLTNSTAIDTSPSYSPDGSKVVFTSDRGGRAQI
YVMGADGSGQTRISFGDGVYSTPVWSPRGDLIAFTKQTGGEFQIGVMKTDGSGERILSSGFQQEGPTWAPNGRVLMFFRD
SNGGPKLVSVDLTGRNEQPIPTANFASDPAWSPLLE

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=415, Percent_Identity=29.6385542168675, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_RHILO (Q98F84)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104897.1
- ProteinModelPortal:   Q98F84
- SMR:   Q98F84
- GeneID:   1227558
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3888
- NMPDR:   fig|266835.1.peg.3001
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK05137
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 47681; Mature: 47681

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFLKPLLTIAAMALGMTAVIPMPAWALVELNVNKGNVEPLPIAITDFQGGDALGAQIS
CHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHH
QIVTADLKRSGLFAPIDKSAFIEKISNPDAAPRFDDWKVINAQALVTGSVSKEADGRIRA
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEEE
QYRLWDTFAGQQMSGEQFFANDANQRRVAHIIADAIYERLTGEKGYFDTRVVFIDESGAK
EEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
NARKKRLAIMDQDGANVRYLSDGRSIVLTPRFSPNRQEITYMSYESGQPRVYLLQIETGQ
CHHHCEEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEECCCC
RELVGNFPGMTFAPRFSPDGQKVIMSLLRDDGNSNIFAMDLRSRSTTRLTNSTAIDTSPS
HHHHCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCEEECCCCC
YSPDGSKVVFTSDRGGRAQIYVMGADGSGQTRISFGDGVYSTPVWSPRGDLIAFTKQTGG
CCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCC
EFQIGVMKTDGSGERILSSGFQQEGPTWAPNGRVLMFFRDSNGGPKLVSVDLTGRNEQPI
EEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCC
PTANFASDPAWSPLLE
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRSFLKPLLTIAAMALGMTAVIPMPAWALVELNVNKGNVEPLPIAITDFQGGDALGAQIS
CHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHH
QIVTADLKRSGLFAPIDKSAFIEKISNPDAAPRFDDWKVINAQALVTGSVSKEADGRIRA
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEEE
QYRLWDTFAGQQMSGEQFFANDANQRRVAHIIADAIYERLTGEKGYFDTRVVFIDESGAK
EEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
NARKKRLAIMDQDGANVRYLSDGRSIVLTPRFSPNRQEITYMSYESGQPRVYLLQIETGQ
CHHHCEEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEECCCC
RELVGNFPGMTFAPRFSPDGQKVIMSLLRDDGNSNIFAMDLRSRSTTRLTNSTAIDTSPS
HHHHCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCEEECCCCC
YSPDGSKVVFTSDRGGRAQIYVMGADGSGQTRISFGDGVYSTPVWSPRGDLIAFTKQTGG
CCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCC
EFQIGVMKTDGSGERILSSGFQQEGPTWAPNGRVLMFFRDSNGGPKLVSVDLTGRNEQPI
EEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCC
PTANFASDPAWSPLLE
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968