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The map label for this gene is tilS

Identifier: 13473327

GI number: 13473327

Start: 3121908

End: 3123272

Strand: Reverse

Name: tilS

Synonym: mll3884

Alternate gene names: 13473327

Gene position: 3123272-3121908 (Counterclockwise)

Preceding gene: 13473328

Following gene: 13473326

Centisome position: 44.39

GC content: 68.06

Gene sequence:

>1365_bases
ATGCTCGACACCGAGCCTGACCTTTCGACCAGACTTTTTTCGCATATCGATTTTACCCACGGCGCCGTTGCGGCCGTGTC
CGGCGGGAGCGACTCGACCGCCCTGCTTCTCCTTCTCAAGCACCATTTCGACCGAACCGGATCGTCCGCGAAGCTGCTGG
CGGTGACAATCGACCATGGATTGCGTCAGGGTTCGGCAGCCGAGGCGCAAGCCGTGGCGAAGCTGTGCGCGGAGCGCGGC
ATTGCCCACCGCACCTTGACGTGGACCGGGCGCAAGCCATCGACCGGCCTGCCGGCGGCGGCGCGGGACGCGCGCTACCA
TCTGCTGGCCGAGGCGGCGCGCGCGGAAGGCATCGGCCTGATCGTCACCGGTCATACCGCCGACGACCAAGCCGAGACGG
TGTTGATGCGGCATGCACGGGACCGGGAGCTTGCCGACCTGGCGGGTCGTGGGCTTGCCGGTATGGCACCGGCCACCCTC
TATGATTGGCGCGAGTGGATCGTTCGGCCGCTTCTGGGCACGCGGCGCTCGGCGCTTCGCGATTTCCTGCGGCGCGAACA
TGTCGGCTGGGCCGAGGACCCGACCAATATCGACGAAGCCTTCGAACGGCCGCGCGTACGCGCGGCACTTGCCGGCGAAA
GTGCCGGGCACATGGAGAATACGCTGCGGCTCGCCGGCCAGGCGGCCGTCGAGCGCGGGCAGCTTGGCGCCAGCGCCGCC
AATCTGATCCGTCGCATTGCCAGCCAGCCGAGCACGGGCCTCATCCGGCTCGATCCTGCTCTGCTGATCGCGGACGACCA
GGCGGCCATCTATGCGTTGCGCATCTTGCTGGCCACCGCCGGAGGCGCTGCCTTCCTGCCGGACCAGGCGCGCAGCGCGG
CGCTGTTCGTCCGGCTGAAGGCCGGATTTCTTTGCGCCACATTGTCGCGGACGGTGGTCGATTTCCGGCACGCCGGTCTC
TTCCTGCGCCGCGAGGCGCGGGGTTTGCCGCCCGCCGCCACCGCTGTCGACAACACCATCTGGGACGGCCGCCGCCACAT
CACGTTGAACGACATGTCCGGCGCATTATTGATCGCCCCGTTGGGTGCGGCAGCAGCGAGGCGGCTGGCGATCGATGATG
GAGAAACCCCGGCAAGCCTGATACGCGCCGCACTGGCCGCGGAACCGACATTGCTGCAAGCCTTTGAAAACCTTGGTTTG
CCGCAGGGCGAGCCGCGCTTACCGGTGTTCGCGGCGCGACCGGTCGTGTCGCCTTTTGCCCGCTTCCTGCCGTCCTTCGA
TCTGGCGCCGGCGCGGGCCGTCGCCGAACTGATCGGCGCGTCACCGCTTCCCGCTTCGCCATTGGGCGGCCACAGTGCCG
ATTGA

Upstream 100 bases:

>100_bases
AAGGAACGCGTCAAGCAGGAGAAGGCCCTGGCTGCGTGCTGATTTTTCCCAGGCATGTCTTTGTCCCAGGACCGCGACCA
CTTTCGGACGACATGCGTTG

Downstream 100 bases:

>100_bases
TGGTGGTCGAAAGCTTAACCCACACATGCTGTTCTGCTTGGCAAGGGGAGACGCTCTCCCTATGTTAGGCCCAAGTTTCC
TCTCATGATGCTGTCCGTGC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MLDTEPDLSTRLFSHIDFTHGAVAAVSGGSDSTALLLLLKHHFDRTGSSAKLLAVTIDHGLRQGSAAEAQAVAKLCAERG
IAHRTLTWTGRKPSTGLPAAARDARYHLLAEAARAEGIGLIVTGHTADDQAETVLMRHARDRELADLAGRGLAGMAPATL
YDWREWIVRPLLGTRRSALRDFLRREHVGWAEDPTNIDEAFERPRVRAALAGESAGHMENTLRLAGQAAVERGQLGASAA
NLIRRIASQPSTGLIRLDPALLIADDQAAIYALRILLATAGGAAFLPDQARSAALFVRLKAGFLCATLSRTVVDFRHAGL
FLRREARGLPPAATAVDNTIWDGRRHITLNDMSGALLIAPLGAAAARRLAIDDGETPASLIRAALAAEPTLLQAFENLGL
PQGEPRLPVFAARPVVSPFARFLPSFDLAPARAVAELIGASPLPASPLGGHSAD

Sequences:

>Translated_454_residues
MLDTEPDLSTRLFSHIDFTHGAVAAVSGGSDSTALLLLLKHHFDRTGSSAKLLAVTIDHGLRQGSAAEAQAVAKLCAERG
IAHRTLTWTGRKPSTGLPAAARDARYHLLAEAARAEGIGLIVTGHTADDQAETVLMRHARDRELADLAGRGLAGMAPATL
YDWREWIVRPLLGTRRSALRDFLRREHVGWAEDPTNIDEAFERPRVRAALAGESAGHMENTLRLAGQAAVERGQLGASAA
NLIRRIASQPSTGLIRLDPALLIADDQAAIYALRILLATAGGAAFLPDQARSAALFVRLKAGFLCATLSRTVVDFRHAGL
FLRREARGLPPAATAVDNTIWDGRRHITLNDMSGALLIAPLGAAAARRLAIDDGETPASLIRAALAAEPTLLQAFENLGL
PQGEPRLPVFAARPVVSPFARFLPSFDLAPARAVAELIGASPLPASPLGGHSAD
>Mature_454_residues
MLDTEPDLSTRLFSHIDFTHGAVAAVSGGSDSTALLLLLKHHFDRTGSSAKLLAVTIDHGLRQGSAAEAQAVAKLCAERG
IAHRTLTWTGRKPSTGLPAAARDARYHLLAEAARAEGIGLIVTGHTADDQAETVLMRHARDRELADLAGRGLAGMAPATL
YDWREWIVRPLLGTRRSALRDFLRREHVGWAEDPTNIDEAFERPRVRAALAGESAGHMENTLRLAGQAAVERGQLGASAA
NLIRRIASQPSTGLIRLDPALLIADDQAAIYALRILLATAGGAAFLPDQARSAALFVRLKAGFLCATLSRTVVDFRHAGL
FLRREARGLPPAATAVDNTIWDGRRHITLNDMSGALLIAPLGAAAARRLAIDDGETPASLIRAALAAEPTLLQAFENLGL
PQGEPRLPVFAARPVVSPFARFLPSFDLAPARAVAELIGASPLPASPLGGHSAD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_RHILO (Q98F87)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104894.1
- ProteinModelPortal:   Q98F87
- SMR:   Q98F87
- GeneID:   1227555
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3884
- NMPDR:   fig|266835.1.peg.2998
- HOGENOM:   HBG354873
- OMA:   DDQRETL
- ProtClustDB:   CLSK506008
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 48159; Mature: 48159

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTEPDLSTRLFSHIDFTHGAVAAVSGGSDSTALLLLLKHHFDRTGSSAKLLAVTIDHG
CCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
LRQGSAAEAQAVAKLCAERGIAHRTLTWTGRKPSTGLPAAARDARYHLLAEAARAEGIGL
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IVTGHTADDQAETVLMRHARDRELADLAGRGLAGMAPATLYDWREWIVRPLLGTRRSALR
EEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DFLRREHVGWAEDPTNIDEAFERPRVRAALAGESAGHMENTLRLAGQAAVERGQLGASAA
HHHHHHCCCCCCCCCCHHHHHHCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
NLIRRIASQPSTGLIRLDPALLIADDQAAIYALRILLATAGGAAFLPDQARSAALFVRLK
HHHHHHHCCCCCCEEEECCEEEEECCHHHHHHHHHHHHHCCCCEECCCHHCCEEEEEEEH
AGFLCATLSRTVVDFRHAGLFLRREARGLPPAATAVDNTIWDGRRHITLNDMSGALLIAP
HCHHHHHHHHHHHHHHHHCHHEEHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEE
LGAAAARRLAIDDGETPASLIRAALAAEPTLLQAFENLGLPQGEPRLPVFAARPVVSPFA
CCHHHHHHEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHH
RFLPSFDLAPARAVAELIGASPLPASPLGGHSAD
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDTEPDLSTRLFSHIDFTHGAVAAVSGGSDSTALLLLLKHHFDRTGSSAKLLAVTIDHG
CCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
LRQGSAAEAQAVAKLCAERGIAHRTLTWTGRKPSTGLPAAARDARYHLLAEAARAEGIGL
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IVTGHTADDQAETVLMRHARDRELADLAGRGLAGMAPATLYDWREWIVRPLLGTRRSALR
EEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DFLRREHVGWAEDPTNIDEAFERPRVRAALAGESAGHMENTLRLAGQAAVERGQLGASAA
HHHHHHCCCCCCCCCCHHHHHHCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
NLIRRIASQPSTGLIRLDPALLIADDQAAIYALRILLATAGGAAFLPDQARSAALFVRLK
HHHHHHHCCCCCCEEEECCEEEEECCHHHHHHHHHHHHHCCCCEECCCHHCCEEEEEEEH
AGFLCATLSRTVVDFRHAGLFLRREARGLPPAATAVDNTIWDGRRHITLNDMSGALLIAP
HCHHHHHHHHHHHHHHHHCHHEEHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEE
LGAAAARRLAIDDGETPASLIRAALAAEPTLLQAFENLGLPQGEPRLPVFAARPVVSPFA
CCHHHHHHEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHH
RFLPSFDLAPARAVAELIGASPLPASPLGGHSAD
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968