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The map label for this gene is thiN [H]

Identifier: 13473313

GI number: 13473313

Start: 3104211

End: 3104855

Strand: Direct

Name: thiN [H]

Synonym: mlr3869

Alternate gene names: 13473313

Gene position: 3104211-3104855 (Clockwise)

Preceding gene: 13473307

Following gene: 13473314

Centisome position: 44.12

GC content: 68.84

Gene sequence:

>645_bases
ATGAGCACATTCACCATCCTGCTTGGCGGCGATCTCATCCGCACGCCCCGGCTCGACCGCCAGGTCGAAGGCTCGCGCGT
CATCGCCGCCGACGCCGGCATCGGCCATGCCCGGCTGCTCGGCCTTGTGCCGGAATTGTGGGTGGGCGATTTCGATTCCG
TGCCGACCGATCTGCCCGCCGACCTTGCCGCGGTGCCCCGCCGGACTTTCCCGGCGGAGAAGGACAAGACCGACGGCGAA
CTGGCGATCGCCGCAGCACTCGAGCGCGGCGCGACCCGCCTTGTGCTGGCGGGTGCGTTCGGCGGCAAGCGCGCCGACCA
CGCCTTCCTGCATCTGGCGCTCGCCCTGCGCCTGGCCGAGGCCGGAACGGAGGTGCTGCTGACCAGTGGCGCCCAGGAAG
GTGTCCCGCTGCTGCTGGGAACGGCCGGCTTCGACTATGCCGACGGCACGCTGTTTTCGATCCTCGGCTTTTCCGAACTT
GCCGGCCTGACCGTCACCGGCGCCAAATGGCCGTTGAACCAGGTCGAGGTGGCCTTCGGCTCGTCGCTGACCATTTCCAA
CGAGGTCCGGGGTCGCCTGGACATCGCGCTCGGCCACGGCCGCGCGCTGCTGCTCGCGCATCCGTATCCACTACCTGAAA
GCTGA

Upstream 100 bases:

>100_bases
CGTTAGAGCGTTTCGACACATAGGCCGGGCCGAGGAACCGCGCAAGTGGCAGTTTGCCGGCCTTCCGTTTCCGCTGCCGG
GCTGGTACAGGCCACCGGAT

Downstream 100 bases:

>100_bases
CATGGCCCCGCCCCTTCTCAATCTCGACGGGATAAGACTGACCTTTGGCGGCACACCGCTGCTCGACGGCGCCGCGTTGA
CCGCTTCGGCCGGCGACAAG

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSTFTILLGGDLIRTPRLDRQVEGSRVIAADAGIGHARLLGLVPELWVGDFDSVPTDLPADLAAVPRRTFPAEKDKTDGE
LAIAAALERGATRLVLAGAFGGKRADHAFLHLALALRLAEAGTEVLLTSGAQEGVPLLLGTAGFDYADGTLFSILGFSEL
AGLTVTGAKWPLNQVEVAFGSSLTISNEVRGRLDIALGHGRALLLAHPYPLPES

Sequences:

>Translated_214_residues
MSTFTILLGGDLIRTPRLDRQVEGSRVIAADAGIGHARLLGLVPELWVGDFDSVPTDLPADLAAVPRRTFPAEKDKTDGE
LAIAAALERGATRLVLAGAFGGKRADHAFLHLALALRLAEAGTEVLLTSGAQEGVPLLLGTAGFDYADGTLFSILGFSEL
AGLTVTGAKWPLNQVEVAFGSSLTISNEVRGRLDIALGHGRALLLAHPYPLPES
>Mature_213_residues
STFTILLGGDLIRTPRLDRQVEGSRVIAADAGIGHARLLGLVPELWVGDFDSVPTDLPADLAAVPRRTFPAEKDKTDGEL
AIAAALERGATRLVLAGAFGGKRADHAFLHLALALRLAEAGTEVLLTSGAQEGVPLLLGTAGFDYADGTLFSILGFSELA
GLTVTGAKWPLNQVEVAFGSSLTISNEVRGRLDIALGHGRALLLAHPYPLPES

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 22397; Mature: 22266

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFTILLGGDLIRTPRLDRQVEGSRVIAADAGIGHARLLGLVPELWVGDFDSVPTDLPA
CCEEEEEECCCEEECCCCCCCCCCCEEEEECCCCCHHHHHHHCHHHHCCCCCCCCCCCCC
DLAAVPRRTFPAEKDKTDGELAIAAALERGATRLVLAGAFGGKRADHAFLHLALALRLAE
HHHHCCCCCCCCCCCCCCCCEEEEEHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
AGTEVLLTSGAQEGVPLLLGTAGFDYADGTLFSILGFSELAGLTVTGAKWPLNQVEVAFG
CCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEECC
SSLTISNEVRGRLDIALGHGRALLLAHPYPLPES
CCEEECCCCCCEEEEEECCCCEEEEECCCCCCCC
>Mature Secondary Structure 
STFTILLGGDLIRTPRLDRQVEGSRVIAADAGIGHARLLGLVPELWVGDFDSVPTDLPA
CEEEEEECCCEEECCCCCCCCCCCEEEEECCCCCHHHHHHHCHHHHCCCCCCCCCCCCC
DLAAVPRRTFPAEKDKTDGELAIAAALERGATRLVLAGAFGGKRADHAFLHLALALRLAE
HHHHCCCCCCCCCCCCCCCCEEEEEHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
AGTEVLLTSGAQEGVPLLLGTAGFDYADGTLFSILGFSELAGLTVTGAKWPLNQVEVAFG
CCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEECC
SSLTISNEVRGRLDIALGHGRALLLAHPYPLPES
CCEEECCCCCCEEEEEECCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]