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The map label for this gene is rpoH2 [H]

Identifier: 13473307

GI number: 13473307

Start: 3097723

End: 3098586

Strand: Direct

Name: rpoH2 [H]

Synonym: mlr3862

Alternate gene names: 13473307

Gene position: 3097723-3098586 (Clockwise)

Preceding gene: 13473303

Following gene: 13473313

Centisome position: 44.03

GC content: 62.73

Gene sequence:

>864_bases
ATGATGGAAGACACGGCAGGACGGATCATGGTCCGCGCGGCGATGAAAGCTCCCTATCTCGAACGCGACGAGGAACATCG
GCTGGCCTTGCTCTGGAAGCAAGACAACGACCAGAACGCATTGCACAGCATCACTGTCGCCCATATGCGGCTGGTGATTT
CCATGGCCTCGAAATTCCGCCATTACGGCCTGCCGCTCGGCGACCTGATCCAGGAAGGCCATGTTGGCCTGCTCGAGGCC
GCCGCCCGCTTCGAGCCCGAACGCGAAGTACGGTTTTCGACCTATGCGACGTGGTGGATCCGCGCCTCGATGCAGGACTA
CATCCTGCGCAACTGGTCGATCGTGCGCGGCGGAACCAGTTCCGCGCAGAAAGCCCTGTTCTTCAACCTGCGCCGCCTGC
GCGCCCGGCTGGCGAATGGCGCCGAGCCGCTTTCCAACACGACCCTCTATCGCGAAGTATCGGTGGCGCTTGGCGTCTCC
GAGGCTGACGTGGCGATGATGGATTCACGGCTGTCGGCGCCCGACTCCTCGCTCAACATGCCGCTTGCCGACGATGCGGG
TGCCACCGAGCGGATGGACTTCCTGGTTTCCGATGATCCCCTGCCTGACGAGATTGTCGGCGACAAGATCGATGTCGCGC
GCCGTTCACTCTGGTTGAAAGAAGCCCTTCACGCGCTCAACGCGCGGGAACTCAGGATCATCGAGGAACGCCGGCTGAAC
GACGAGGGCGCCACGCTCGAGGCACTCGGCGAGACGCTCGGCATTTCCAAGGAACGCGTCCGCCAGATCGAGGCCCGCGC
CATGGAAAAGCTCAAGGTGGCGCTGGTGAAACAGAACCCGGAATTTCTGGCGACAGCCGCGTGA

Upstream 100 bases:

>100_bases
ATGGCAGTTTTGGCTGTTCTAGGGCGCGACGCCGCGATTTCAACCGGCGCCCTGCCAAGTTCGTAACGTCGGTTGTATCG
CCTCAGCCAGGGAGCGCCTC

Downstream 100 bases:

>100_bases
CTCCCGCGTAACAGCGCTTACTTGTCGGTGACGATCTTGACCTTGTCGCCGGCCGAAACCGCGGCGCCCGGCGACAACGC
GTTGAGCACCCGGAACAGGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MMEDTAGRIMVRAAMKAPYLERDEEHRLALLWKQDNDQNALHSITVAHMRLVISMASKFRHYGLPLGDLIQEGHVGLLEA
AARFEPEREVRFSTYATWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLRRLRARLANGAEPLSNTTLYREVSVALGVS
EADVAMMDSRLSAPDSSLNMPLADDAGATERMDFLVSDDPLPDEIVGDKIDVARRSLWLKEALHALNARELRIIEERRLN
DEGATLEALGETLGISKERVRQIEARAMEKLKVALVKQNPEFLATAA

Sequences:

>Translated_287_residues
MMEDTAGRIMVRAAMKAPYLERDEEHRLALLWKQDNDQNALHSITVAHMRLVISMASKFRHYGLPLGDLIQEGHVGLLEA
AARFEPEREVRFSTYATWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLRRLRARLANGAEPLSNTTLYREVSVALGVS
EADVAMMDSRLSAPDSSLNMPLADDAGATERMDFLVSDDPLPDEIVGDKIDVARRSLWLKEALHALNARELRIIEERRLN
DEGATLEALGETLGISKERVRQIEARAMEKLKVALVKQNPEFLATAA
>Mature_287_residues
MMEDTAGRIMVRAAMKAPYLERDEEHRLALLWKQDNDQNALHSITVAHMRLVISMASKFRHYGLPLGDLIQEGHVGLLEA
AARFEPEREVRFSTYATWWIRASMQDYILRNWSIVRGGTSSAQKALFFNLRRLRARLANGAEPLSNTTLYREVSVALGVS
EADVAMMDSRLSAPDSSLNMPLADDAGATERMDFLVSDDPLPDEIVGDKIDVARRSLWLKEALHALNARELRIIEERRLN
DEGATLEALGETLGISKERVRQIEARAMEKLKVALVKQNPEFLATAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=271, Percent_Identity=38.0073800738007, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789098, Length=237, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=30.2040816326531, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32298; Mature: 32298

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEDTAGRIMVRAAMKAPYLERDEEHRLALLWKQDNDQNALHSITVAHMRLVISMASKFR
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HYGLPLGDLIQEGHVGLLEAAARFEPEREVRFSTYATWWIRASMQDYILRNWSIVRGGTS
HCCCCHHHHHHCCCCHHHHHHHHCCCCHHEEHHHEEEEEEHHHHHHHHHHCCEEEECCCC
SAQKALFFNLRRLRARLANGAEPLSNTTLYREVSVALGVSEADVAMMDSRLSAPDSSLNM
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
PLADDAGATERMDFLVSDDPLPDEIVGDKIDVARRSLWLKEALHALNARELRIIEERRLN
CCCCCCCCHHHHEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DEGATLEALGETLGISKERVRQIEARAMEKLKVALVKQNPEFLATAA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECC
>Mature Secondary Structure
MMEDTAGRIMVRAAMKAPYLERDEEHRLALLWKQDNDQNALHSITVAHMRLVISMASKFR
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HYGLPLGDLIQEGHVGLLEAAARFEPEREVRFSTYATWWIRASMQDYILRNWSIVRGGTS
HCCCCHHHHHHCCCCHHHHHHHHCCCCHHEEHHHEEEEEEHHHHHHHHHHCCEEEECCCC
SAQKALFFNLRRLRARLANGAEPLSNTTLYREVSVALGVSEADVAMMDSRLSAPDSSLNM
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
PLADDAGATERMDFLVSDDPLPDEIVGDKIDVARRSLWLKEALHALNARELRIIEERRLN
CCCCCCCCHHHHEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DEGATLEALGETLGISKERVRQIEARAMEKLKVALVKQNPEFLATAA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]