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The map label for this gene is ispG

Identifier: 13473254

GI number: 13473254

Start: 3047431

End: 3048684

Strand: Reverse

Name: ispG

Synonym: mll3792

Alternate gene names: 13473254

Gene position: 3048684-3047431 (Counterclockwise)

Preceding gene: 13473255

Following gene: 13473253

Centisome position: 43.33

GC content: 64.67

Gene sequence:

>1254_bases
ATGACCGGATATTTTTCCTTTCCCTTCCCCCGCCGCACCTCGGTCGGCGTCGATGTCGGCGGCGTGGCCGTCGGCGGCGG
CGCGCCTGTCGTGGTGCAGTCAATGACCAACACCGACACGGCCGATATCGATCAGACCGTCGCGCAGGTCGCGGCACTGC
ACCGCGCCGGCTCCGAGATCGTGCGCATAACCGTCGACCGCGACGAGAGCGCCGCCGCCGTGCCGCGCATTCACGAAAGG
CTTCAGCGGCTCGGTATCAATGTGCCGCTGGTCGGCGACTTCCACTATATCGGCCACAAGCTGCTCGCCGATCATCCGGC
CTGTGCCGAGGCACTGGCCAAATACCGCATCAATCCGGGCAATGTCGGCTTCAAGGACAAGAAGGACCGGCAGTTCACCG
ACATCGTCGAAATGGCGATCAAACACGACAAGCCTGTGCGCATCGGCGTCAACTGGGGTTCGCTTGACCAGGAGCTTCTG
ACCCGACTGATGGACGACAACCAGGACAAGGGCTTTCCGCTGACGGCGCAGGAAGTGACGCGCGAGGCGATCGTGCAGTC
CGCCATCCTGTCGGCCGAGATGGCCGAGGAGATCGGCCTCGGCCGCGACAAGATCATCCTGTCGGCCAAGGTCAGCGGCG
TGCAGGACCTGATTGCCGTCTACACCGAGCTTGCCACCCGCTCCGACCATGCGCTGCATCTCGGCCTCACCGAGGCCGGC
ATGGGCTCGAAAGGCATCGTCGCCTCGTCCGCCGCCATGGGCATCCTCTTGCAGCAAGGCATCGGCGACACCATCCGCAT
CTCGCTGACGCCGGAGCCGAATGGCGACCGCACGCGCGAGGTGCAGGTGGCGCAGGAACTGCTGCAGACCATGGGTTTCA
GGCAGTTCGTGCCGATCGTCGCCGCTTGTCCCGGCTGCGGCCGCACGACCTCCACCGTGTTCCAGGAGCTTGCCCAGAAC
ATCCAGGCTGATCTGCGCAAGAACATGCCGGTGTGGCGCGAAAAATATCCCGGCGTCGAGAACCTCAAGGTCGCGGTGAT
GGGCTGCATCGTCAACGGCCCGGGCGAATCCAAGCATGCCGATATCGGCATTTCGCTGCCCGGCACCGGCGAGACGCCGA
CGGCGCCTGTCTTCGTCGACGGCAAGAAGGCGGCGACGCTGCGCGGAACGTCGATCGCGGCGGATTTCGAGAAAATGGTC
ACCGACTATATCGAGCAGAGGTTTGGGCGCGGCGGCAAAGCCGCGGCAGAGTAG

Upstream 100 bases:

>100_bases
TGCTCCTCCACCTCATGCCCGGACTTCAGCGCATCGGCCGGGAGCGCACCTTGTGCGTCCAAGCGGACGCACGGCGCCCC
AGAGACAAAAGGCTGCCGGC

Downstream 100 bases:

>100_bases
ACGAAATGCAGCGTTTCCTCAGGGTGACGCTGGTTCTGCTTTGCGGGCTGGCCTGGTCGACGCTTGCCCAGGCGGATCCG
CCGCAATCGGCCAAGCAGCG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTGYFSFPFPRRTSVGVDVGGVAVGGGAPVVVQSMTNTDTADIDQTVAQVAALHRAGSEIVRITVDRDESAAAVPRIHER
LQRLGINVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDRQFTDIVEMAIKHDKPVRIGVNWGSLDQELL
TRLMDDNQDKGFPLTAQEVTREAIVQSAILSAEMAEEIGLGRDKIILSAKVSGVQDLIAVYTELATRSDHALHLGLTEAG
MGSKGIVASSAAMGILLQQGIGDTIRISLTPEPNGDRTREVQVAQELLQTMGFRQFVPIVAACPGCGRTTSTVFQELAQN
IQADLRKNMPVWREKYPGVENLKVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFVDGKKAATLRGTSIAADFEKMV
TDYIEQRFGRGGKAAAE

Sequences:

>Translated_417_residues
MTGYFSFPFPRRTSVGVDVGGVAVGGGAPVVVQSMTNTDTADIDQTVAQVAALHRAGSEIVRITVDRDESAAAVPRIHER
LQRLGINVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDRQFTDIVEMAIKHDKPVRIGVNWGSLDQELL
TRLMDDNQDKGFPLTAQEVTREAIVQSAILSAEMAEEIGLGRDKIILSAKVSGVQDLIAVYTELATRSDHALHLGLTEAG
MGSKGIVASSAAMGILLQQGIGDTIRISLTPEPNGDRTREVQVAQELLQTMGFRQFVPIVAACPGCGRTTSTVFQELAQN
IQADLRKNMPVWREKYPGVENLKVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFVDGKKAATLRGTSIAADFEKMV
TDYIEQRFGRGGKAAAE
>Mature_416_residues
TGYFSFPFPRRTSVGVDVGGVAVGGGAPVVVQSMTNTDTADIDQTVAQVAALHRAGSEIVRITVDRDESAAAVPRIHERL
QRLGINVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDRQFTDIVEMAIKHDKPVRIGVNWGSLDQELLT
RLMDDNQDKGFPLTAQEVTREAIVQSAILSAEMAEEIGLGRDKIILSAKVSGVQDLIAVYTELATRSDHALHLGLTEAGM
GSKGIVASSAAMGILLQQGIGDTIRISLTPEPNGDRTREVQVAQELLQTMGFRQFVPIVAACPGCGRTTSTVFQELAQNI
QADLRKNMPVWREKYPGVENLKVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFVDGKKAATLRGTSIAADFEKMVT
DYIEQRFGRGGKAAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=398, Percent_Identity=32.9145728643216, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_RHILO (Q98FG0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104821.1
- ProteinModelPortal:   Q98FG0
- GeneID:   1227482
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3792
- NMPDR:   fig|266835.1.peg.2925
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 44706; Mature: 44574

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGYFSFPFPRRTSVGVDVGGVAVGGGAPVVVQSMTNTDTADIDQTVAQVAALHRAGSEI
CCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEE
VRITVDRDESAAAVPRIHERLQRLGINVPLVGDFHYIGHKLLADHPACAEALAKYRINPG
EEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCHHHHHHHHHHCCCCC
NVGFKDKKDRQFTDIVEMAIKHDKPVRIGVNWGSLDQELLTRLMDDNQDKGFPLTAQEVT
CCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
REAIVQSAILSAEMAEEIGLGRDKIILSAKVSGVQDLIAVYTELATRSDHALHLGLTEAG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECHHCC
MGSKGIVASSAAMGILLQQGIGDTIRISLTPEPNGDRTREVQVAQELLQTMGFRQFVPIV
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
AACPGCGRTTSTVFQELAQNIQADLRKNMPVWREKYPGVENLKVAVMGCIVNGPGESKHA
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEEEEECCCCCCCCC
DIGISLPGTGETPTAPVFVDGKKAATLRGTSIAADFEKMVTDYIEQRFGRGGKAAAE
EEEEECCCCCCCCCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TGYFSFPFPRRTSVGVDVGGVAVGGGAPVVVQSMTNTDTADIDQTVAQVAALHRAGSEI
CCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEE
VRITVDRDESAAAVPRIHERLQRLGINVPLVGDFHYIGHKLLADHPACAEALAKYRINPG
EEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCHHHHHHHHHHCCCCC
NVGFKDKKDRQFTDIVEMAIKHDKPVRIGVNWGSLDQELLTRLMDDNQDKGFPLTAQEVT
CCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
REAIVQSAILSAEMAEEIGLGRDKIILSAKVSGVQDLIAVYTELATRSDHALHLGLTEAG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECHHCC
MGSKGIVASSAAMGILLQQGIGDTIRISLTPEPNGDRTREVQVAQELLQTMGFRQFVPIV
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
AACPGCGRTTSTVFQELAQNIQADLRKNMPVWREKYPGVENLKVAVMGCIVNGPGESKHA
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEEEEECCCCCCCCC
DIGISLPGTGETPTAPVFVDGKKAATLRGTSIAADFEKMVTDYIEQRFGRGGKAAAE
EEEEECCCCCCCCCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968