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The map label for this gene is pgk

Identifier: 13473223

GI number: 13473223

Start: 3021881

End: 3023077

Strand: Direct

Name: pgk

Synonym: mlr3753

Alternate gene names: 13473223

Gene position: 3021881-3023077 (Clockwise)

Preceding gene: 13473222

Following gene: 13473224

Centisome position: 42.95

GC content: 66.67

Gene sequence:

>1197_bases
ATGGCCGCCTTCAAGACACTGGACGATATCGGCAACATCAGCGGCAAGCGCGTGCTGGTGCGCGTCGACCTCAACGTTCC
CGTCGCCGACGGCAAGGTCACCGACGCCACCCGCATCGAACGCATCGCGCCGACCATCGCCGAACTGTCGGGCAAGGGCG
CCAAGGTCATCCTGCTCGCCCATTTCGGCCGGCCCAAGGATGGGCCATCCCCGGAATTCTCGCTGGAGCCGATCGCCAGG
GCGACGGCCGAGGTGCTCGGCCGTCCCGTCGGCTTTGCCTCGGATTGCGTCGGCGACATGGCGGGAAGTGCTGTCGCGGC
CATGAACAAGGGTGACGTGCTGCTCTTCGAAAATACCCGTTTCTACAAGGCCGAGGAGAAGAACGACCCGGCCTTCAGCG
AGCGGCTCGCCGCCAATGGCGACATTTTCGTCAACGACGCCTTTTCCGCGGCGCACCGCGCCCACTCTTCCACAGAGGGG
CTGGCGCGTCTGTTGCCTTCCTTTGCTGGCCGCACCATGCAGGCCGAGCTCGAAGCGCTGGAGAAGGGCCTGGGCAACCC
GGTCCGCCCGGTCGTGGCGATCGTCGGCGGCGCCAAGGTCTCGACCAAGATCGACCTGCTGATGAACCTGGTGAAGAAGG
TCGACGCGCTGGTCATCGGCGGCGGCATGGCCAACACCTTCCTTGCCGCGCGCGGCACCGATGTCGGCAAGTCGCTGTGC
GAGCATGATCTTGCCCCCACCGCCAAGCAGATCATGATCGAGGCCGCCGAGGCCGGCTGCGCCATCATCCTGCCGGTCGA
CGGTGTCGTCGCCAAGGAATTCAAGGCGGGCGCCGCCTGCGAGACCGTCGCCATCTCCGACGTGCCGGCCGATGGCATGA
TCCTGGATGTCGGCGAAAAGACCGTGAAGACGATCGGCGAATGGATCGACCGCGCCGCGACACTGGTCTGGAACGGCCCG
CTTGGCGCCTTCGAGATCGAGCCGTTCGATCACGCCACGGTGGCGGCGGCAAAACACGCGGCCGCGCGCACCAAGGCCGG
CAAGCTGGTCTCCGTTGCGGGTGGCGGCGACACGGTGGCGGCGCTCAACCACGCCGGCGTCGCCGACGACTTCACCTATG
TCTCTACCGCCGGCGGCGCCTTCCTGGAATGGATGGAAGGCAAGCCTCTGCCAGGCGTCGACGTGCTGAAGCGCTGA

Upstream 100 bases:

>100_bases
CTGCCCGCCTGAAAATTTCGCATCGCGTCGCGGCCAAAGCCTTGCATCGTCACCGATGCGGGGTATGGTCCCGGCGATTT
TCCCGAGGAAAGGAACCCGC

Downstream 100 bases:

>100_bases
GGCGCGATCAACACAAAGAGGCGCCCGGGACTTTCAATCGATTCGAGCTTTCCGATGCCATTCCTTTGGCGGTAGATTTC
CGTTAAGCGCGGAACGAACC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAAFKTLDDIGNISGKRVLVRVDLNVPVADGKVTDATRIERIAPTIAELSGKGAKVILLAHFGRPKDGPSPEFSLEPIAR
ATAEVLGRPVGFASDCVGDMAGSAVAAMNKGDVLLFENTRFYKAEEKNDPAFSERLAANGDIFVNDAFSAAHRAHSSTEG
LARLLPSFAGRTMQAELEALEKGLGNPVRPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFLAARGTDVGKSLC
EHDLAPTAKQIMIEAAEAGCAIILPVDGVVAKEFKAGAACETVAISDVPADGMILDVGEKTVKTIGEWIDRAATLVWNGP
LGAFEIEPFDHATVAAAKHAAARTKAGKLVSVAGGGDTVAALNHAGVADDFTYVSTAGGAFLEWMEGKPLPGVDVLKR

Sequences:

>Translated_398_residues
MAAFKTLDDIGNISGKRVLVRVDLNVPVADGKVTDATRIERIAPTIAELSGKGAKVILLAHFGRPKDGPSPEFSLEPIAR
ATAEVLGRPVGFASDCVGDMAGSAVAAMNKGDVLLFENTRFYKAEEKNDPAFSERLAANGDIFVNDAFSAAHRAHSSTEG
LARLLPSFAGRTMQAELEALEKGLGNPVRPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFLAARGTDVGKSLC
EHDLAPTAKQIMIEAAEAGCAIILPVDGVVAKEFKAGAACETVAISDVPADGMILDVGEKTVKTIGEWIDRAATLVWNGP
LGAFEIEPFDHATVAAAKHAAARTKAGKLVSVAGGGDTVAALNHAGVADDFTYVSTAGGAFLEWMEGKPLPGVDVLKR
>Mature_397_residues
AAFKTLDDIGNISGKRVLVRVDLNVPVADGKVTDATRIERIAPTIAELSGKGAKVILLAHFGRPKDGPSPEFSLEPIARA
TAEVLGRPVGFASDCVGDMAGSAVAAMNKGDVLLFENTRFYKAEEKNDPAFSERLAANGDIFVNDAFSAAHRAHSSTEGL
ARLLPSFAGRTMQAELEALEKGLGNPVRPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFLAARGTDVGKSLCE
HDLAPTAKQIMIEAAEAGCAIILPVDGVVAKEFKAGAACETVAISDVPADGMILDVGEKTVKTIGEWIDRAATLVWNGPL
GAFEIEPFDHATVAAAKHAAARTKAGKLVSVAGGGDTVAALNHAGVADDFTYVSTAGGAFLEWMEGKPLPGVDVLKR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=41.8491484184915, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=40.8759124087591, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1789294, Length=389, Percent_Identity=45.5012853470437, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17508823, Length=403, Percent_Identity=42.6799007444169, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6319857, Length=403, Percent_Identity=39.454094292804, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24581203, Length=404, Percent_Identity=41.0891089108911, Blast_Score=264, Evalue=8e-71,
Organism=Drosophila melanogaster, GI17136394, Length=403, Percent_Identity=40.1985111662531, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_RHILO (Q98FJ1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104790.1
- ProteinModelPortal:   Q98FJ1
- SMR:   Q98FJ1
- GeneID:   1227451
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr3753
- NMPDR:   fig|266835.1.peg.2894
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41305; Mature: 41174

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 120-120 BINDING 153-153 BINDING 203-203 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFKTLDDIGNISGKRVLVRVDLNVPVADGKVTDATRIERIAPTIAELSGKGAKVILLA
CCCHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
HFGRPKDGPSPEFSLEPIARATAEVLGRPVGFASDCVGDMAGSAVAAMNKGDVLLFENTR
ECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCEEEEECCE
FYKAEEKNDPAFSERLAANGDIFVNDAFSAAHRAHSSTEGLARLLPSFAGRTMQAELEAL
EEECCCCCCCHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
EKGLGNPVRPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFLAARGTDVGKSLC
HHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHEECCCHHHHHHH
EHDLAPTAKQIMIEAAEAGCAIILPVDGVVAKEFKAGAACETVAISDVPADGMILDVGEK
HHCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCEEEECCCCCCCEEEECCHH
TVKTIGEWIDRAATLVWNGPLGAFEIEPFDHATVAAAKHAAARTKAGKLVSVAGGGDTVA
HHHHHHHHHHHHEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEE
ALNHAGVADDFTYVSTAGGAFLEWMEGKPLPGVDVLKR
EECCCCCCCCCCEEECCCCHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
AAFKTLDDIGNISGKRVLVRVDLNVPVADGKVTDATRIERIAPTIAELSGKGAKVILLA
CCHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
HFGRPKDGPSPEFSLEPIARATAEVLGRPVGFASDCVGDMAGSAVAAMNKGDVLLFENTR
ECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCEEEEECCE
FYKAEEKNDPAFSERLAANGDIFVNDAFSAAHRAHSSTEGLARLLPSFAGRTMQAELEAL
EEECCCCCCCHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
EKGLGNPVRPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFLAARGTDVGKSLC
HHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHEECCCHHHHHHH
EHDLAPTAKQIMIEAAEAGCAIILPVDGVVAKEFKAGAACETVAISDVPADGMILDVGEK
HHCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCEEEECCCCCCCEEEECCHH
TVKTIGEWIDRAATLVWNGPLGAFEIEPFDHATVAAAKHAAARTKAGKLVSVAGGGDTVA
HHHHHHHHHHHHEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEE
ALNHAGVADDFTYVSTAGGAFLEWMEGKPLPGVDVLKR
EECCCCCCCCCCEEECCCCHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968