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The map label for this gene is ubiE [H]

Identifier: 13473166

GI number: 13473166

Start: 2965741

End: 2966535

Strand: Reverse

Name: ubiE [H]

Synonym: mll3672

Alternate gene names: 13473166

Gene position: 2966535-2965741 (Counterclockwise)

Preceding gene: 13473171

Following gene: 13473165

Centisome position: 42.16

GC content: 65.41

Gene sequence:

>795_bases
ATGATCGAAACGGCAGATGCCGAGTATGACGACACCGCCATCCGCTTCCTCGAGGCGCTGTGGGGCGATGGCTACCTGTC
TCCGGGCGGACCTGAAGAGGTCGACCGGGTCGTCGAAGGGCTTTCGCTCGAGGGCAAGACGGTTCTCGATATCGGCTGCG
GCTCCGGCGGCATCACCTTGCATCTGGTCGAGCGCCATGGCGCTGCCCGCGCCACCGGTTTCGATGTCGAACAGCCAGTG
ATCGAGGCGGCCAGGCGGCGAGCTGCCGGGCGCGGGCTCTCGGACCGCGCCAGCTTCGTCCAGGCGCCACCCGGAGCGCT
GCCTTTCGCCGATCGCTCCTTCGATGCCGTTTTTTCCAAGGACGCGCTGCTGCATGTGCCAGACAAGGACGGCGTGTTCG
CCGAAATCTTCCGCGTGCTGAAGCCGGGTGGCGTCTTTGCCGCCTCCAACTGGATGATCGGGCATGACAGTGAACCGTCG
CCCGAGATGAAGGCCTATATCGCCGCCGAAGGGCTGTCCTTCGCCATGGCCTCGCCGGCGCGCTATGCGCACGCGATGCG
CAACGCCGGCTTTGCCGATGTCACCGTCAGGGACCGCAACCCCTGGTATCGCGAGGTGGCGCGCGGCGAGCTGGAAAGGC
TGAAAGGACCGCTCTATACGCCGGTCGCCGCCGTGGTCGGCGCTGCCTATGTCGACAAGAACATCCGAACCTGGGAGGCC
ATGCAGAAGGTGCTTGACAGTGGCGAGCACCGTCCCACTCATCTGCGCGGTTGGAAGCCGGATGCGATTGGATAG

Upstream 100 bases:

>100_bases
ACTGAATGAATGTTCAACAAATGATGTTGCGTTTTCCCAGACATGCGCTAGGCTTTGCCGGCGTTCGAACTGAGCATTCA
TCGGTTGGGATCGAGGGCTG

Downstream 100 bases:

>100_bases
CCGGCGATGCTGGAGACGATCACACCCATGAAAACGGCAGACGCCAAGGATGCCCTCCTTGAACCGGCGGGGAAAGCCGA
GACCGAAAAGCGCGGACGCA

Product: methyl transferase-like protein

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIETADAEYDDTAIRFLEALWGDGYLSPGGPEEVDRVVEGLSLEGKTVLDIGCGSGGITLHLVERHGAARATGFDVEQPV
IEAARRRAAGRGLSDRASFVQAPPGALPFADRSFDAVFSKDALLHVPDKDGVFAEIFRVLKPGGVFAASNWMIGHDSEPS
PEMKAYIAAEGLSFAMASPARYAHAMRNAGFADVTVRDRNPWYREVARGELERLKGPLYTPVAAVVGAAYVDKNIRTWEA
MQKVLDSGEHRPTHLRGWKPDAIG

Sequences:

>Translated_264_residues
MIETADAEYDDTAIRFLEALWGDGYLSPGGPEEVDRVVEGLSLEGKTVLDIGCGSGGITLHLVERHGAARATGFDVEQPV
IEAARRRAAGRGLSDRASFVQAPPGALPFADRSFDAVFSKDALLHVPDKDGVFAEIFRVLKPGGVFAASNWMIGHDSEPS
PEMKAYIAAEGLSFAMASPARYAHAMRNAGFADVTVRDRNPWYREVARGELERLKGPLYTPVAAVVGAAYVDKNIRTWEA
MQKVLDSGEHRPTHLRGWKPDAIG
>Mature_264_residues
MIETADAEYDDTAIRFLEALWGDGYLSPGGPEEVDRVVEGLSLEGKTVLDIGCGSGGITLHLVERHGAARATGFDVEQPV
IEAARRRAAGRGLSDRASFVQAPPGALPFADRSFDAVFSKDALLHVPDKDGVFAEIFRVLKPGGVFAASNWMIGHDSEPS
PEMKAYIAAEGLSFAMASPARYAHAMRNAGFADVTVRDRNPWYREVARGELERLKGPLYTPVAAVVGAAYVDKNIRTWEA
MQKVLDSGEHRPTHLRGWKPDAIG

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=101, Percent_Identity=33.6633663366337, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561380, Length=212, Percent_Identity=29.2452830188679, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25143945, Length=151, Percent_Identity=31.7880794701987, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323635, Length=172, Percent_Identity=33.1395348837209, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28605; Mature: 28605

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETADAEYDDTAIRFLEALWGDGYLSPGGPEEVDRVVEGLSLEGKTVLDIGCGSGGITL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
HLVERHGAARATGFDVEQPVIEAARRRAAGRGLSDRASFVQAPPGALPFADRSFDAVFSK
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCHHHHHCC
DALLHVPDKDGVFAEIFRVLKPGGVFAASNWMIGHDSEPSPEMKAYIAAEGLSFAMASPA
CCEEECCCCCCHHHHHHHHHCCCCEEEECCEEECCCCCCCHHHHEEHHHCCCHHHHCCHH
RYAHAMRNAGFADVTVRDRNPWYREVARGELERLKGPLYTPVAAVVGAAYVDKNIRTWEA
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
MQKVLDSGEHRPTHLRGWKPDAIG
HHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIETADAEYDDTAIRFLEALWGDGYLSPGGPEEVDRVVEGLSLEGKTVLDIGCGSGGITL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
HLVERHGAARATGFDVEQPVIEAARRRAAGRGLSDRASFVQAPPGALPFADRSFDAVFSK
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCHHHHHCC
DALLHVPDKDGVFAEIFRVLKPGGVFAASNWMIGHDSEPSPEMKAYIAAEGLSFAMASPA
CCEEECCCCCCHHHHHHHHHCCCCEEEECCEEECCCCCCCHHHHEEHHHCCCHHHHCCHH
RYAHAMRNAGFADVTVRDRNPWYREVARGELERLKGPLYTPVAAVVGAAYVDKNIRTWEA
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
MQKVLDSGEHRPTHLRGWKPDAIG
HHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]