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The map label for this gene is prfA

Identifier: 13472970

GI number: 13472970

Start: 2771149

End: 2772228

Strand: Reverse

Name: prfA

Synonym: mll3435

Alternate gene names: 13472970

Gene position: 2772228-2771149 (Counterclockwise)

Preceding gene: 13472971

Following gene: 13472969

Centisome position: 39.4

GC content: 63.7

Gene sequence:

>1080_bases
ATGGTCAATCTGCCCCGCGATCGTATGGATCAAGTCGTCAAGCGTTTCGAGATGCTCGAAGCGCAGATGTCGGCCGGCCC
GGCGCCGGACGCCTATGTGAAGATGGCATCGGAATATGCCGAGCTGCAGGACATGGTGGCCAAGGTCAGGCAATTGCGCT
CGGCCGAGCATGAGCAGGCCGACCTCGAGGCGATGCTGGCCGACAAAGGTACCGATGCCGAGATGCGGGCGCTGGCCGAG
GCGGATCTGCCTGACGTGGAGGAGCGCATCGAGGCGCTGCAGAAGGACATCCAGATCCTGCTTCTGCCCAAGGATGCCGC
CGACGACAAGAACGCCATCCTCGAAATCCGTGCCGGCACCGGCGGCGATGAAGCCGCTCTTTTCGCCGGCGACCTGTTTC
GTATGTATGAGCGCTATGCCGCCGAACGCGGCTGGCGGTTCGAGACGGTGTCGGCCAGCGACGGCGATGCCGGCGGGTTC
AAGGAAATCATCGCCACCGTCTCGGGCAAGGGCGTGTTTGCCCATTTGAAATTCGAATCCGGCGTGCATCGCGTGCAACG
CGTGCCGGCGACCGAGGCCAGTGGGCGCATTCACACCTCGGCGGCGACCGTCGCCGTGCTGCCGGAAGCGGAAGAGGTCG
ACATTGAAATCCGCGCAGAAGACATCCGCATCGACACGATGCGCGCTTCGGGCTCCGGCGGCCAGCACGTCAACACCACC
GATTCGGCGGTGCGCATCACCCATTTGCCGACCGGCATCATGGTGGTGCAGGCGGAGAAGTCGCAGCACCAGAACCGGGC
CAAGGCCATGCAGATCCTGCGTGCCCGGCTCTATGATCTGGAGCGCAGCAAGGCGGACGAGGAACGCTCGGAATCGCGCA
AATCGCAGGTCGGCTCCGGTGACCGCTCCGAGCGCATCCGCACCTATAATTTCCCGCAGGGCCGCGTCACCGACCATCGC
ATCAACCTGACGCTCTACAAGCTCGACCGGGTGATGATGGGCGAACTCGACGAGATCGTCGACGCGCTGATCGCCGATCA
CCAGTCGAAGCTGTTGGCCGATATCGGCCTTGATGGCTGA

Upstream 100 bases:

>100_bases
TTCGACGACAATCTGATGGCGCCGGCGCAGGGGCTGCCGATGCGGGCGCTGCTGCAGGCCTTCGCCGACGACCGCTCGAT
TCCGTTGTAGCGCCCCCATC

Downstream 100 bases:

>100_bases
CCCTCTGCCCGAGGCGCTGGGGCCGCTGCTGCGGGAAGCGCGGGCCAGGCTTGTGGCGGCCGGGGTCGGCGATCCGGCGC
TCGATGCGCGGCTGATCGTC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MVNLPRDRMDQVVKRFEMLEAQMSAGPAPDAYVKMASEYAELQDMVAKVRQLRSAEHEQADLEAMLADKGTDAEMRALAE
ADLPDVEERIEALQKDIQILLLPKDAADDKNAILEIRAGTGGDEAALFAGDLFRMYERYAAERGWRFETVSASDGDAGGF
KEIIATVSGKGVFAHLKFESGVHRVQRVPATEASGRIHTSAATVAVLPEAEEVDIEIRAEDIRIDTMRASGSGGQHVNTT
DSAVRITHLPTGIMVVQAEKSQHQNRAKAMQILRARLYDLERSKADEERSESRKSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMMGELDEIVDALIADHQSKLLADIGLDG

Sequences:

>Translated_359_residues
MVNLPRDRMDQVVKRFEMLEAQMSAGPAPDAYVKMASEYAELQDMVAKVRQLRSAEHEQADLEAMLADKGTDAEMRALAE
ADLPDVEERIEALQKDIQILLLPKDAADDKNAILEIRAGTGGDEAALFAGDLFRMYERYAAERGWRFETVSASDGDAGGF
KEIIATVSGKGVFAHLKFESGVHRVQRVPATEASGRIHTSAATVAVLPEAEEVDIEIRAEDIRIDTMRASGSGGQHVNTT
DSAVRITHLPTGIMVVQAEKSQHQNRAKAMQILRARLYDLERSKADEERSESRKSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMMGELDEIVDALIADHQSKLLADIGLDG
>Mature_359_residues
MVNLPRDRMDQVVKRFEMLEAQMSAGPAPDAYVKMASEYAELQDMVAKVRQLRSAEHEQADLEAMLADKGTDAEMRALAE
ADLPDVEERIEALQKDIQILLLPKDAADDKNAILEIRAGTGGDEAALFAGDLFRMYERYAAERGWRFETVSASDGDAGGF
KEIIATVSGKGVFAHLKFESGVHRVQRVPATEASGRIHTSAATVAVLPEAEEVDIEIRAEDIRIDTMRASGSGGQHVNTT
DSAVRITHLPTGIMVVQAEKSQHQNRAKAMQILRARLYDLERSKADEERSESRKSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMMGELDEIVDALIADHQSKLLADIGLDG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=47.9099678456592, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI34577120, Length=347, Percent_Identity=38.328530259366, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=47.1153846153846, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1787462, Length=326, Percent_Identity=57.361963190184, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=34.8973607038123, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=307, Percent_Identity=39.7394136807818, Blast_Score=204, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6321295, Length=336, Percent_Identity=42.8571428571429, Blast_Score=249, Evalue=7e-67,
Organism=Drosophila melanogaster, GI19921226, Length=349, Percent_Identity=39.541547277937, Blast_Score=231, Evalue=6e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_RHILO (Q98G93)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104537.1
- ProteinModelPortal:   Q98G93
- SMR:   Q98G93
- GeneID:   1227198
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3435
- NMPDR:   fig|266835.1.peg.2641
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39746; Mature: 39746

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLPRDRMDQVVKRFEMLEAQMSAGPAPDAYVKMASEYAELQDMVAKVRQLRSAEHEQA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DLEAMLADKGTDAEMRALAEADLPDVEERIEALQKDIQILLLPKDAADDKNAILEIRAGT
HHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCC
GGDEAALFAGDLFRMYERYAAERGWRFETVSASDGDAGGFKEIIATVSGKGVFAHLKFES
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEHH
GVHRVQRVPATEASGRIHTSAATVAVLPEAEEVDIEIRAEDIRIDTMRASGSGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCCEEEEEECEEEEEEEEECCCCCCCCCCC
DSAVRITHLPTGIMVVQAEKSQHQNRAKAMQILRARLYDLERSKADEERSESRKSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMMGELDEIVDALIADHQSKLLADIGLDG
CCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNLPRDRMDQVVKRFEMLEAQMSAGPAPDAYVKMASEYAELQDMVAKVRQLRSAEHEQA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DLEAMLADKGTDAEMRALAEADLPDVEERIEALQKDIQILLLPKDAADDKNAILEIRAGT
HHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCC
GGDEAALFAGDLFRMYERYAAERGWRFETVSASDGDAGGFKEIIATVSGKGVFAHLKFES
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEHH
GVHRVQRVPATEASGRIHTSAATVAVLPEAEEVDIEIRAEDIRIDTMRASGSGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCCEEEEEECEEEEEEEEECCCCCCCCCCC
DSAVRITHLPTGIMVVQAEKSQHQNRAKAMQILRARLYDLERSKADEERSESRKSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMMGELDEIVDALIADHQSKLLADIGLDG
CCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968