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The map label for this gene is hemK

Identifier: 13472969

GI number: 13472969

Start: 2770284

End: 2771156

Strand: Reverse

Name: hemK

Synonym: mll3434

Alternate gene names: 13472969

Gene position: 2771156-2770284 (Counterclockwise)

Preceding gene: 13472970

Following gene: 13472968

Centisome position: 39.38

GC content: 65.29

Gene sequence:

>873_bases
ATGGCTGACCCTCTGCCCGAGGCGCTGGGGCCGCTGCTGCGGGAAGCGCGGGCCAGGCTTGTGGCGGCCGGGGTCGGCGA
TCCGGCGCTCGATGCGCGGCTGATCGTCGAGCATTTTTCCGGCACGACGCGCACACAGGCCATTGCCGACCCTGAACGCA
CGATCGACAGCAACGCCATCGCCGCGATCGATGCGGCCCTGGGACGCCGCGCGGGTGGCGAACCGGTGCACCGCATCCTC
GGCTACCGCGAATTCTACGGTTTGCGCCTGTCGCTGTCGCCGGAAACGCTCGAGCCGAGGCCGGATACCGAAACGCTGGT
CGAGGCGGTGCTGCCCTTCGTCAAGGCGATGGCCGCGCGAGAGGGGACCTGCCGCATCCTTGATCTCGGTACCGGGACGG
GCGCCATCGCGCTGGCGCTGCTGAGCGCCGTGCCGGCCGCGACCGCCACCGGGGTCGACATTTCCGCCGGCGCGCTGGCG
ACGGCGGCCCGCAATGCCGGGGAACTCGGGCTCGGCGGCCGGTTCACGACGGTGCAGTCGGACTGGTTCGAAAAAGTTTC
CGGCCGATACCATGTAATTGCCGCAAACCCTCCCTATATACCGACAAGAGACATTGGAAATCTGCAGGACGAGGTCCGCG
ATTTCGACCCACGCCTGGCCCTGGATGGCGGCGTGGATGGGCTGAACCCCTACAGGATCATTGCCGCCGAGGCGGCACGG
TTTCTAGAAGCCGAAAGCAGGATTGCGGTCGAAATCGGCCATACGCAACGCGATGAGGTTACGGATATATTCAAGGCGGC
TGGTTATGCATCGGTTGCTGCTTTGCGTGATCTCGGCGGAAACGACAGGGTTCTTGTCTTTCAATGGGGATAA

Upstream 100 bases:

>100_bases
CTCTACAAGCTCGACCGGGTGATGATGGGCGAACTCGACGAGATCGTCGACGCGCTGATCGCCGATCACCAGTCGAAGCT
GTTGGCCGATATCGGCCTTG

Downstream 100 bases:

>100_bases
GCCTTGACGCAGTGCGAAAAAACCGCTTGGCAATACCCGGGAATGCGGCTAGGGTCGCCTCAACCGGATGAGACGAAGCA
GGCAGTGCTCTTAGCGATTC

Product: protoporphyrinogen oxidase, hemK protein

Products: NA

Alternate protein names: M.MloHemKP

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MADPLPEALGPLLREARARLVAAGVGDPALDARLIVEHFSGTTRTQAIADPERTIDSNAIAAIDAALGRRAGGEPVHRIL
GYREFYGLRLSLSPETLEPRPDTETLVEAVLPFVKAMAAREGTCRILDLGTGTGAIALALLSAVPAATATGVDISAGALA
TAARNAGELGLGGRFTTVQSDWFEKVSGRYHVIAANPPYIPTRDIGNLQDEVRDFDPRLALDGGVDGLNPYRIIAAEAAR
FLEAESRIAVEIGHTQRDEVTDIFKAAGYASVAALRDLGGNDRVLVFQWG

Sequences:

>Translated_290_residues
MADPLPEALGPLLREARARLVAAGVGDPALDARLIVEHFSGTTRTQAIADPERTIDSNAIAAIDAALGRRAGGEPVHRIL
GYREFYGLRLSLSPETLEPRPDTETLVEAVLPFVKAMAAREGTCRILDLGTGTGAIALALLSAVPAATATGVDISAGALA
TAARNAGELGLGGRFTTVQSDWFEKVSGRYHVIAANPPYIPTRDIGNLQDEVRDFDPRLALDGGVDGLNPYRIIAAEAAR
FLEAESRIAVEIGHTQRDEVTDIFKAAGYASVAALRDLGGNDRVLVFQWG
>Mature_289_residues
ADPLPEALGPLLREARARLVAAGVGDPALDARLIVEHFSGTTRTQAIADPERTIDSNAIAAIDAALGRRAGGEPVHRILG
YREFYGLRLSLSPETLEPRPDTETLVEAVLPFVKAMAAREGTCRILDLGTGTGAIALALLSAVPAATATGVDISAGALAT
AARNAGELGLGGRFTTVQSDWFEKVSGRYHVIAANPPYIPTRDIGNLQDEVRDFDPRLALDGGVDGLNPYRIIAAEAARF
LEAESRIAVEIGHTQRDEVTDIFKAAGYASVAALRDLGGNDRVLVFQWG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=276, Percent_Identity=31.1594202898551, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1787463, Length=273, Percent_Identity=40.2930402930403, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082085, Length=132, Percent_Identity=38.6363636363636, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324266, Length=172, Percent_Identity=31.3953488372093, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582226, Length=245, Percent_Identity=31.0204081632653, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_RHILO (Q98G94)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104536.1
- ProteinModelPortal:   Q98G94
- SMR:   Q98G94
- GeneID:   1227197
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3434
- NMPDR:   fig|266835.1.peg.2640
- HOGENOM:   HBG732041
- OMA:   DPRPETE
- ProtClustDB:   PRK09328
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 30689; Mature: 30558

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 153-153 BINDING 182-182 BINDING 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPLPEALGPLLREARARLVAAGVGDPALDARLIVEHFSGTTRTQAIADPERTIDSNAI
CCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHH
AAIDAALGRRAGGEPVHRILGYREFYGLRLSLSPETLEPRPDTETLVEAVLPFVKAMAAR
HHHHHHHHCCCCCCHHHHHHCHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGTCRILDLGTGTGAIALALLSAVPAATATGVDISAGALATAARNAGELGLGGRFTTVQS
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECHH
DWFEKVSGRYHVIAANPPYIPTRDIGNLQDEVRDFDPRLALDGGVDGLNPYRIIAAEAAR
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHH
FLEAESRIAVEIGHTQRDEVTDIFKAAGYASVAALRDLGGNDRVLVFQWG
HHHHCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
ADPLPEALGPLLREARARLVAAGVGDPALDARLIVEHFSGTTRTQAIADPERTIDSNAI
CCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHH
AAIDAALGRRAGGEPVHRILGYREFYGLRLSLSPETLEPRPDTETLVEAVLPFVKAMAAR
HHHHHHHHCCCCCCHHHHHHCHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGTCRILDLGTGTGAIALALLSAVPAATATGVDISAGALATAARNAGELGLGGRFTTVQS
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECHH
DWFEKVSGRYHVIAANPPYIPTRDIGNLQDEVRDFDPRLALDGGVDGLNPYRIIAAEAAR
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHH
FLEAESRIAVEIGHTQRDEVTDIFKAAGYASVAALRDLGGNDRVLVFQWG
HHHHCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968