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The map label for this gene is gatA [H]

Identifier: 13472921

GI number: 13472921

Start: 2708116

End: 2709411

Strand: Reverse

Name: gatA [H]

Synonym: mll3370

Alternate gene names: 13472921

Gene position: 2709411-2708116 (Counterclockwise)

Preceding gene: 13472923

Following gene: 13472915

Centisome position: 38.51

GC content: 65.74

Gene sequence:

>1296_bases
ATGCAATCCATCCGCGACCGTCTCGAAATCATCCTGTCACGTCTTGCCAATCGTGCGGCCGACGAAAAGGTCTTCACCAA
GCTCTATATCGAGGCCGCGCGGGCCGCGGCTGACGCCAGCGACGCCCGCAACCGAGCCGGGGTGACGCTCGGACCGCTCG
ACGGCGTCATCGTCTCGATAAAGGATCTGTTCGACGTCGCCAGCGAAGTGACCACGGCCGGCTCGCTGATGCGCAAGACA
GCCGCACCGGCTATGCGCGATGCCGCTATCGTCAGCCGGCTTCGGCAAGCCGGCGCGGTCATCATCGGCAAGACCAACAT
GACAGAATTCGCCTTCACGGCGATCGGCGACAACATGCATTACGGCACGCCCGGCAACGCCATCGATGCCAGCCGTATTC
CCGGCGGTTCGTCCTCAGGCGCCGGCGTTTCCGTCGGTGAGGGGACGAGCGACATCTCCATCGGATCCGATACCGGTGGC
TCGATCCGCATTCCGGCATCGCTCAATAGTGTCGTCGGTTTCAAGCCGACGGCGCGGCGGGTGCCGCTGATCGGCGCCTT
TCCGCTATCTGGCACTTTGGACTCGATCGGGCCGCTTGCCCGAACCGTCGCGCAATGTGCCGCCACAGACGCGGTGATGG
CCGGCGAGGCGCCGGCCGAACTGGCGGCCATATCGCTCGCGGGACTTCGAGTCGGTGTGCCGCGTGGTGTTCTTTTTGGC
GACACGGAAGGTGAAGTTGCCGCGGCGTTCGAGCATTGTCTCGGCAAGATCGAACAGGCCGGCGCGCGCATGGCCGATCT
GGTAATCGACGACATGATCGCAGAGTTCAGGACCGCCACCAAGCGTGGCTCGATCGCTGCCATGGAAGGGGCCGAAGTGC
ATGCCGACTGGCTGGCGACAGGGGCATCGGTGCCGGTCGACCCGCATGTCAGCGGGCCTTTGTCGCGGGCGGCAATGCTG
CCGGTGCCCGTCTATATCAGGGTCCTGCGCCGCCGCAGCGCTCTCATTGCCGCCATGGACGACCGGTTGGCGTCGGTCGA
TGTGCTGGCCCTGCCGACCACGCCGGTGACGGCGCCGACCATCGTTTCCCTGGCCGAGGATGCCGAACTGCGTGACCATG
TCGAAGGCCTGCTGCTGCGCAACACGCAGGTGGCCAACCAGTTCGACCTCTGCGCGATCTCGCTGCCGATGCCCGACATG
GCGCTGCCGGCCGGACTGATGCTGGTGGCGCGCAACGGCCATGACCGGCGTCTGCTGCGGATAGCCGCCGAAGTGGAACG
GCTGCTTGGTGGCTGA

Upstream 100 bases:

>100_bases
GCTGGCAGGTGCAAAGGCACGCTGGCCGGGTTATCCCTTCCGCAAGCCTATCGACCGCTTGCCTAAAAGACGTTGCATCC
TGGCGGAGTCCGACATCGAC

Downstream 100 bases:

>100_bases
CCAGTCGTGAATCGCAATCGCGGATCAGGGCGATGAACAGTGCTTGCGTTCGCTGTCCTTGGCGGTGCGTACGATACGGG
TCGCGACCAGCCATGGCAGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQSIRDRLEIILSRLANRAADEKVFTKLYIEAARAAADASDARNRAGVTLGPLDGVIVSIKDLFDVASEVTTAGSLMRKT
AAPAMRDAAIVSRLRQAGAVIIGKTNMTEFAFTAIGDNMHYGTPGNAIDASRIPGGSSSGAGVSVGEGTSDISIGSDTGG
SIRIPASLNSVVGFKPTARRVPLIGAFPLSGTLDSIGPLARTVAQCAATDAVMAGEAPAELAAISLAGLRVGVPRGVLFG
DTEGEVAAAFEHCLGKIEQAGARMADLVIDDMIAEFRTATKRGSIAAMEGAEVHADWLATGASVPVDPHVSGPLSRAAML
PVPVYIRVLRRRSALIAAMDDRLASVDVLALPTTPVTAPTIVSLAEDAELRDHVEGLLLRNTQVANQFDLCAISLPMPDM
ALPAGLMLVARNGHDRRLLRIAAEVERLLGG

Sequences:

>Translated_431_residues
MQSIRDRLEIILSRLANRAADEKVFTKLYIEAARAAADASDARNRAGVTLGPLDGVIVSIKDLFDVASEVTTAGSLMRKT
AAPAMRDAAIVSRLRQAGAVIIGKTNMTEFAFTAIGDNMHYGTPGNAIDASRIPGGSSSGAGVSVGEGTSDISIGSDTGG
SIRIPASLNSVVGFKPTARRVPLIGAFPLSGTLDSIGPLARTVAQCAATDAVMAGEAPAELAAISLAGLRVGVPRGVLFG
DTEGEVAAAFEHCLGKIEQAGARMADLVIDDMIAEFRTATKRGSIAAMEGAEVHADWLATGASVPVDPHVSGPLSRAAML
PVPVYIRVLRRRSALIAAMDDRLASVDVLALPTTPVTAPTIVSLAEDAELRDHVEGLLLRNTQVANQFDLCAISLPMPDM
ALPAGLMLVARNGHDRRLLRIAAEVERLLGG
>Mature_431_residues
MQSIRDRLEIILSRLANRAADEKVFTKLYIEAARAAADASDARNRAGVTLGPLDGVIVSIKDLFDVASEVTTAGSLMRKT
AAPAMRDAAIVSRLRQAGAVIIGKTNMTEFAFTAIGDNMHYGTPGNAIDASRIPGGSSSGAGVSVGEGTSDISIGSDTGG
SIRIPASLNSVVGFKPTARRVPLIGAFPLSGTLDSIGPLARTVAQCAATDAVMAGEAPAELAAISLAGLRVGVPRGVLFG
DTEGEVAAAFEHCLGKIEQAGARMADLVIDDMIAEFRTATKRGSIAAMEGAEVHADWLATGASVPVDPHVSGPLSRAAML
PVPVYIRVLRRRSALIAAMDDRLASVDVLALPTTPVTAPTIVSLAEDAELRDHVEGLLLRNTQVANQFDLCAISLPMPDM
ALPAGLMLVARNGHDRRLLRIAAEVERLLGG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=471, Percent_Identity=26.1146496815287, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI195972892, Length=168, Percent_Identity=37.5, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=450, Percent_Identity=28, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=215, Percent_Identity=35.8139534883721, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=159, Percent_Identity=38.9937106918239, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=156, Percent_Identity=32.0512820512821, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=132, Percent_Identity=35.6060606060606, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=184, Percent_Identity=31.5217391304348, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=385, Percent_Identity=27.2727272727273, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323950, Length=208, Percent_Identity=32.6923076923077, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=183, Percent_Identity=38.2513661202186, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=29.2841648590022, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24652985, Length=217, Percent_Identity=35.0230414746544, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922090, Length=217, Percent_Identity=35.0230414746544, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652981, Length=217, Percent_Identity=35.0230414746544, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652983, Length=217, Percent_Identity=35.0230414746544, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550774, Length=203, Percent_Identity=30.0492610837438, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648435, Length=203, Percent_Identity=30.0492610837438, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648437, Length=203, Percent_Identity=30.0492610837438, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648441, Length=203, Percent_Identity=30.0492610837438, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648439, Length=203, Percent_Identity=30.0492610837438, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI161078093, Length=425, Percent_Identity=26.5882352941176, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356731, Length=155, Percent_Identity=34.8387096774194, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644968, Length=224, Percent_Identity=29.9107142857143, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 44751; Mature: 44751

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIRDRLEIILSRLANRAADEKVFTKLYIEAARAAADASDARNRAGVTLGPLDGVIVSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEECCCCCHHHHH
KDLFDVASEVTTAGSLMRKTAAPAMRDAAIVSRLRQAGAVIIGKTNMTEFAFTAIGDNMH
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCC
YGTPGNAIDASRIPGGSSSGAGVSVGEGTSDISIGSDTGGSIRIPASLNSVVGFKPTARR
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
VPLIGAFPLSGTLDSIGPLARTVAQCAATDAVMAGEAPAELAAISLAGLRVGVPRGVLFG
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCEEEC
DTEGEVAAAFEHCLGKIEQAGARMADLVIDDMIAEFRTATKRGSIAAMEGAEVHADWLAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHC
GASVPVDPHVSGPLSRAAMLPVPVYIRVLRRRSALIAAMDDRLASVDVLALPTTPVTAPT
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHCCEEEEEECCCCCCCHH
IVSLAEDAELRDHVEGLLLRNTQVANQFDLCAISLPMPDMALPAGLMLVARNGHDRRLLR
HHHHHHCHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHH
IAAEVERLLGG
HHHHHHHHHCC
>Mature Secondary Structure
MQSIRDRLEIILSRLANRAADEKVFTKLYIEAARAAADASDARNRAGVTLGPLDGVIVSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEECCCCCHHHHH
KDLFDVASEVTTAGSLMRKTAAPAMRDAAIVSRLRQAGAVIIGKTNMTEFAFTAIGDNMH
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCC
YGTPGNAIDASRIPGGSSSGAGVSVGEGTSDISIGSDTGGSIRIPASLNSVVGFKPTARR
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
VPLIGAFPLSGTLDSIGPLARTVAQCAATDAVMAGEAPAELAAISLAGLRVGVPRGVLFG
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCEEEC
DTEGEVAAAFEHCLGKIEQAGARMADLVIDDMIAEFRTATKRGSIAAMEGAEVHADWLAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHC
GASVPVDPHVSGPLSRAAMLPVPVYIRVLRRRSALIAAMDDRLASVDVLALPTTPVTAPT
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHCCEEEEEECCCCCCCHH
IVSLAEDAELRDHVEGLLLRNTQVANQFDLCAISLPMPDMALPAGLMLVARNGHDRRLLR
HHHHHHCHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHH
IAAEVERLLGG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA