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The map label for this gene is grpE

Identifier: 13472808

GI number: 13472808

Start: 2574141

End: 2574773

Strand: Reverse

Name: grpE

Synonym: mll3220

Alternate gene names: 13472808

Gene position: 2574773-2574141 (Counterclockwise)

Preceding gene: 13472811

Following gene: 13472807

Centisome position: 36.59

GC content: 67.14

Gene sequence:

>633_bases
ATGAGCGACCAGGCAAAAGACGAACGCGCGCCCAGTGAAGCCGAGGCGGCCGAGGCCAATGCCGAGCGCACGGAAGGCAG
CATCGACGGCGACTATGAAGCGCTTGTGCGACTGCTGAAGGAAAATGAAGAGCTGAAGGACCGCGCGCTGCGCGTTGCGG
CCGAGATGGAGAACCTGCGCCGCCGCACCGCGCGCGACGTGCACGACGCGCGCACCTATGCGGTTGCGAATTTCGCCCGC
GACATGCTGTCGGTGTCGGACAATCTGCGCCGCGCGCTGGACGCCATTCCGGCGGAGGCCAAGGCCTCGGGCGACGCCGG
CTTCAAGGCGCTGATCGAAGGCGTCGATCTGACCGAGCGCGCCATGCTGTCGGCGCTGGAACGGCATGGGGTCAAGAAGC
TCGCGCCGGAAGGCGAGAAGTTCGATCCCAATTTCCACCAGGCCATGTTCGAAGTGCCCAATCCCGATGTCCCGGCCAAC
ACCGTGGTCCAGGTCGTGCAGCCGGGCTATTCGATCGGCGAGCGCGTGCTGCGTCCGGCCATGGTCGGCGTCGCCAAGGG
CGGCCCGAAGATTGCCGCCGAGGCGCCGGTCGAGCCGGGGCCGGTGAATGAGCAGGCGGAGAAGGATGCGTAG

Upstream 100 bases:

>100_bases
CCGGCCAAAGGCTCAATGGTCGCTTGCCCCTTGATTTTTGGCTGCCAAACCTCGATATCGGGCACAAAATCCATACCATT
CGAGATGACGGGAAACGGCG

Downstream 100 bases:

>100_bases
TTAGGGGTTAGGCAGTAGGCAGTAGGCAGTAGGCAGTAGGGAATAAGGCAGTCGTATGAATCGCGCGGCTTCGTGCTATT
CATACGCCCCAATTCCCAAT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSDQAKDERAPSEAEAAEANAERTEGSIDGDYEALVRLLKENEELKDRALRVAAEMENLRRRTARDVHDARTYAVANFAR
DMLSVSDNLRRALDAIPAEAKASGDAGFKALIEGVDLTERAMLSALERHGVKKLAPEGEKFDPNFHQAMFEVPNPDVPAN
TVVQVVQPGYSIGERVLRPAMVGVAKGGPKIAAEAPVEPGPVNEQAEKDA

Sequences:

>Translated_210_residues
MSDQAKDERAPSEAEAAEANAERTEGSIDGDYEALVRLLKENEELKDRALRVAAEMENLRRRTARDVHDARTYAVANFAR
DMLSVSDNLRRALDAIPAEAKASGDAGFKALIEGVDLTERAMLSALERHGVKKLAPEGEKFDPNFHQAMFEVPNPDVPAN
TVVQVVQPGYSIGERVLRPAMVGVAKGGPKIAAEAPVEPGPVNEQAEKDA
>Mature_209_residues
SDQAKDERAPSEAEAAEANAERTEGSIDGDYEALVRLLKENEELKDRALRVAAEMENLRRRTARDVHDARTYAVANFARD
MLSVSDNLRRALDAIPAEAKASGDAGFKALIEGVDLTERAMLSALERHGVKKLAPEGEKFDPNFHQAMFEVPNPDVPANT
VVQVVQPGYSIGERVLRPAMVGVAKGGPKIAAEAPVEPGPVNEQAEKDA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=148, Percent_Identity=39.8648648648649, Blast_Score=107, Evalue=5e-24,
Organism=Homo sapiens, GI40255109, Length=156, Percent_Identity=35.8974358974359, Blast_Score=94, Evalue=6e-20,
Organism=Escherichia coli, GI1788967, Length=142, Percent_Identity=40.1408450704225, Blast_Score=106, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=162, Percent_Identity=37.6543209876543, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324806, Length=188, Percent_Identity=39.8936170212766, Blast_Score=126, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24653432, Length=172, Percent_Identity=38.3720930232558, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_RHILO (Q98GQ5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104375.1
- ProteinModelPortal:   Q98GQ5
- SMR:   Q98GQ5
- GeneID:   1227036
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3220
- NMPDR:   fig|266835.1.peg.2479
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14141
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22742; Mature: 22610

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQAKDERAPSEAEAAEANAERTEGSIDGDYEALVRLLKENEELKDRALRVAAEMENLR
CCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RRTARDVHDARTYAVANFARDMLSVSDNLRRALDAIPAEAKASGDAGFKALIEGVDLTER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHCCCHHHH
AMLSALERHGVKKLAPEGEKFDPNFHQAMFEVPNPDVPANTVVQVVQPGYSIGERVLRPA
HHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
MVGVAKGGPKIAAEAPVEPGPVNEQAEKDA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDQAKDERAPSEAEAAEANAERTEGSIDGDYEALVRLLKENEELKDRALRVAAEMENLR
CCCCCHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RRTARDVHDARTYAVANFARDMLSVSDNLRRALDAIPAEAKASGDAGFKALIEGVDLTER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHCCCHHHH
AMLSALERHGVKKLAPEGEKFDPNFHQAMFEVPNPDVPANTVVQVVQPGYSIGERVLRPA
HHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
MVGVAKGGPKIAAEAPVEPGPVNEQAEKDA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968