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The map label for this gene is ysgA [H]

Identifier: 13472801

GI number: 13472801

Start: 2567044

End: 2567868

Strand: Direct

Name: ysgA [H]

Synonym: mlr3210

Alternate gene names: 13472801

Gene position: 2567044-2567868 (Clockwise)

Preceding gene: 13472800

Following gene: 13472802

Centisome position: 36.48

GC content: 64.61

Gene sequence:

>825_bases
GTGAAGGAAGTCACCAGCCTCGCCAATCCACTGATCAAGGACATCAAGGCGCTTGCCCTGAAGAAATTCCGCGACCAGCA
GAACGCCTTCATGGCCGAGGGGCTGAAACTCGTCATTGATGCGCTCGACCTGGGCTGGCAGATCAGGACGCTAGTGTTCG
CCAAGGCCGGACGCGGCAACCCAGCGGTGGAAAAGGTCGCGGCGCGCACGGTTGCCGCCGGCGGCACGGTGCTCGAAGTC
TCGGAAAAAGTGCTTGTCGCCATCACCCGCCGCGACAATCCGCAAATGGTGGTCGGCGTCTTCTCGCAGAAATTCCTGGC
CCTGAAGGACATCCGCGCCGACAATGGCGATGTCTGGGTGGCGCTCGACCGGGTGCGCGATCCCGGCAATCTCGGCACCG
TCATCCGCACCGTCGATGCCGTCGGCGCCAAGGGTGTCATCCTGGTCGGCGACACCACCGATCCATTCTCCGTGGAGACG
GTGCGCGCCACCATGGGTTCGATCTTCGCCGTGCCGGTGGCCAAGGCAACGACCGAGGCCTTCCTTGCCTGGCGCGGCGG
CTTTTCGGGCCTTGTCGCCGGCACGCATCTGAAGGGTGCGGTCGACTACCGCTCGGTCGATTTTTCCCGTGGGCCGGTGC
TGCTGATGATGGGCAATGAGCAACAGGGCCTGCCTGAAAGCCTGGCGGCGAGTTGCGACCGGCTGCTCAGGATCCCGCAA
GCGGGCCGCGCCGATTCGCTCAATCTCGCCGTCGCCACCGGCATCATGCTGTTCGAGATCCGGCGGGGCGCGCTGAAGCT
CGAACCAATCGCCGACCAGCAATGA

Upstream 100 bases:

>100_bases
GATCATCCGCGAAAAGTCCGCCGGCAGGGCGCTTTCGACCTCATTGTTTTCGCGTTGGGTGGCCTGAATGAACGAACGGC
ATGTCGGCGCACCCGGCCAG

Downstream 100 bases:

>100_bases
AGGTTGCCCGCCCGTGAGATCATGGTCCCCCTACGCGCTGCTCGTCGTCGCGGCCATCGCGCTCGATCAGTGGATCAAAC
ATCTGGTCGAGACCGGCCTG

Product: rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKEVTSLANPLIKDIKALALKKFRDQQNAFMAEGLKLVIDALDLGWQIRTLVFAKAGRGNPAVEKVAARTVAAGGTVLEV
SEKVLVAITRRDNPQMVVGVFSQKFLALKDIRADNGDVWVALDRVRDPGNLGTVIRTVDAVGAKGVILVGDTTDPFSVET
VRATMGSIFAVPVAKATTEAFLAWRGGFSGLVAGTHLKGAVDYRSVDFSRGPVLLMMGNEQQGLPESLAASCDRLLRIPQ
AGRADSLNLAVATGIMLFEIRRGALKLEPIADQQ

Sequences:

>Translated_274_residues
MKEVTSLANPLIKDIKALALKKFRDQQNAFMAEGLKLVIDALDLGWQIRTLVFAKAGRGNPAVEKVAARTVAAGGTVLEV
SEKVLVAITRRDNPQMVVGVFSQKFLALKDIRADNGDVWVALDRVRDPGNLGTVIRTVDAVGAKGVILVGDTTDPFSVET
VRATMGSIFAVPVAKATTEAFLAWRGGFSGLVAGTHLKGAVDYRSVDFSRGPVLLMMGNEQQGLPESLAASCDRLLRIPQ
AGRADSLNLAVATGIMLFEIRRGALKLEPIADQQ
>Mature_274_residues
MKEVTSLANPLIKDIKALALKKFRDQQNAFMAEGLKLVIDALDLGWQIRTLVFAKAGRGNPAVEKVAARTVAAGGTVLEV
SEKVLVAITRRDNPQMVVGVFSQKFLALKDIRADNGDVWVALDRVRDPGNLGTVIRTVDAVGAKGVILVGDTTDPFSVET
VRATMGSIFAVPVAKATTEAFLAWRGGFSGLVAGTHLKGAVDYRSVDFSRGPVLLMMGNEQQGLPESLAASCDRLLRIPQ
AGRADSLNLAVATGIMLFEIRRGALKLEPIADQQ

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=147, Percent_Identity=31.9727891156463, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790623, Length=149, Percent_Identity=32.8859060402685, Blast_Score=71, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24666840, Length=267, Percent_Identity=25.4681647940075, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29293; Mature: 29293

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVTSLANPLIKDIKALALKKFRDQQNAFMAEGLKLVIDALDLGWQIRTLVFAKAGRGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCC
PAVEKVAARTVAAGGTVLEVSEKVLVAITRRDNPQMVVGVFSQKFLALKDIRADNGDVWV
HHHHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEEE
ALDRVRDPGNLGTVIRTVDAVGAKGVILVGDTTDPFSVETVRATMGSIFAVPVAKATTEA
EEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCEEEEECHHHHHHH
FLAWRGGFSGLVAGTHLKGAVDYRSVDFSRGPVLLMMGNEQQGLPESLAASCDRLLRIPQ
HHHCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
AGRADSLNLAVATGIMLFEIRRGALKLEPIADQQ
CCCCCCCCHHHHHHHHHEEECCCCEEECCCCCCC
>Mature Secondary Structure
MKEVTSLANPLIKDIKALALKKFRDQQNAFMAEGLKLVIDALDLGWQIRTLVFAKAGRGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCC
PAVEKVAARTVAAGGTVLEVSEKVLVAITRRDNPQMVVGVFSQKFLALKDIRADNGDVWV
HHHHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEEE
ALDRVRDPGNLGTVIRTVDAVGAKGVILVGDTTDPFSVETVRATMGSIFAVPVAKATTEA
EEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCEEEEECHHHHHHH
FLAWRGGFSGLVAGTHLKGAVDYRSVDFSRGPVLLMMGNEQQGLPESLAASCDRLLRIPQ
HHHCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
AGRADSLNLAVATGIMLFEIRRGALKLEPIADQQ
CCCCCCCCHHHHHHHHHEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]